FastQCFastQC Report
Mon 30 Nov 2020
S33.raw.2997.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.2997.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2192
Sequences flagged as poor quality0
Sequence length11-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTTGAAGTCCCGAGTTATCGCTTTCTG1185.383211678832116No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGT1125.109489051094891No Hit
TACTTAATTGTGAACTCATGCCGGGGAA894.060218978102189No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACATC602.737226277372263No Hit
TCCCGAGTTATCGCTTTCTGTAATTGTCTC542.4635036496350367No Hit
TCCATAATTGGCGGTTTGCTGGTAGATA502.281021897810219No Hit
TCTTGAAGTCCCGAGTTATCGCTTTCTG462.0985401459854014No Hit
TAACTCTTCGTCCGACTCGACATCCATA381.7335766423357664No Hit
TACTTAATTGTGAACTCATGCCGGGGAAT311.4142335766423357No Hit
TCCGGGCACAGTTTCTTCACAGGGGACGTC291.322992700729927No Hit
TGCTTCCAGTAGACTTCCTTCGGTTGACCT271.2317518248175183No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGAG251.1405109489051095No Hit
TGCTGATCGGGAGTCAAATGCACTTGCACT251.1405109489051095No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACA241.094890510948905No Hit
TCACAGGGGACGTCGGTCTGGGACACTC221.0036496350364963No Hit
TAATTGGCGGTTTGCTGGTAGATATGGGC221.0036496350364963No Hit
TTCACAGGGGCCGTCGGTCTGGGACACTC200.9124087591240875No Hit
TCACAGGGGCCGTCGGTCTGGGACACTC190.8667883211678832No Hit
TTGCCCCGTCTGCGCACTGGATGAAACCGT180.8211678832116789No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTGA170.7755474452554745No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGAGT160.7299270072992701No Hit
TCTTGAAGTCCCGAGTTATCGCTTTCTGT150.6843065693430658No Hit
TTCACAGGGGACGTCGGTCTGGGACACTC140.6386861313868614No Hit
TTCAACGGCGCAGCAGGGTGGTTCCGAAC140.6386861313868614No Hit
TCGTGTATAACGTGGACGTGGTCGTGGT140.6386861313868614No Hit
TGCTTGCTTCCAGTAGACTTCCTTCGGTT130.5930656934306568No Hit
TTGGCTTTCGAAGGAGACGGATGTGTGCC130.5930656934306568No Hit
TAGATATGGGCACATCTGCACTGGTTGGAT120.5474452554744526No Hit
TACTTAATTGTGAATTCATGCCGGGGAA120.5474452554744526No Hit
TCCTCCCATACTTGCGCTTGCTGATCGGG120.5474452554744526No Hit
TGAAGTCCCGAGTTATCGCTTTCTGTAATT110.5018248175182481No Hit
TTGTCTCAACAGTTGCCCCGTCTGCGCAC110.5018248175182481No Hit
TCCGGGCACAGTTTCTTCACAGGGGCCGTC110.5018248175182481No Hit
TGAGTACAGTTTGAAGAATTCTAGCACCAC110.5018248175182481No Hit
TCTTCACAGGGGCCGTCGGTCTGGGACACTC110.5018248175182481No Hit
TCCGAACGAACTCGAGTAATTGTTCTCCCG100.45620437956204374No Hit
TATTGTTGTAGATCGTCTGGAACTTGGT100.45620437956204374No Hit
TCGGTCTGGGACACTCTTGGAAGGTCAGC100.45620437956204374No Hit
TTCGAGCTTCATTACCAAATCGTGGGATC100.45620437956204374No Hit
TGCTGATCGGGAGTCAAATGCACTTGCACTT90.41058394160583944No Hit
TGGGCACATCTGCACTGGTTGGATTGATA90.41058394160583944No Hit
TGCTTGCTTCCAGTAGACTTCCTTCGGT90.41058394160583944No Hit
TACTTAATTGTGAATTCATGCCGGGGA90.41058394160583944No Hit
TCTCCATAATCCATTAGTGACCAAGCCGCC80.36496350364963503No Hit
TGCCCCGTCTGCGCACTGGATGAAACCG80.36496350364963503No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTG80.36496350364963503No Hit
TTAGTGACCAAGCCGCCAATTCAATTGCT70.3193430656934307No Hit
TTTGAAGAATTCTAGCACCACGATGAAAT70.3193430656934307No Hit
TTGCCCCGTCTGCGCACTGGATGAAACCG70.3193430656934307No Hit
TGAAACCGTGCACAATGTCTTCCAAGTTGC70.3193430656934307No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACAT70.3193430656934307No Hit
TCCAAGTTGCTTGCTTCCAGTAGACTTCCT70.3193430656934307No Hit
TCGACATCCATAATTGGCGGTTTGCTGGT70.3193430656934307No Hit
TTCTGTAATTGTCTCAACAGTTGCCCCGTC70.3193430656934307No Hit
TCAATGGGGAGACTACTTTGAATGAAAAGTC60.2737226277372263No Hit
TCATCTCCAGATTCGCATGCTGACGCGAC60.2737226277372263No Hit
TAATTGGCGGTTTGCTGGTAGATATGGGCACA60.2737226277372263No Hit
TGAAATATGAGTTTCAACGGCGCAGCAGGG60.2737226277372263No Hit
TGAAATAGTGGGGGCAGCAGACCTATAGTCACGCCAGT60.2737226277372263No Hit
TTGTCTCAACAGTTGCCCCGTCTGCGCACT60.2737226277372263No Hit
TGACGGTAAATGCGTTATTAATAACTTTTCGTACTCCTCGTGTATATATT60.2737226277372263No Hit
TAGATATGGGCACATCTGCACTGGTTGGA60.2737226277372263No Hit
TCTGTAATTGTCTCAACAGTTGCCCCGTC60.2737226277372263No Hit
TGCTGGTAGATATGGGCACATCTGCACTG50.22810218978102187No Hit
TTCACAGGGGCCGTCGGTCTGGGACACTCT50.22810218978102187No Hit
TTCGAGCTTCATTACCAAATCGTGGGAT50.22810218978102187No Hit
TTAATTGTGGTGGTCTAGAACTAGGAATT50.22810218978102187No Hit
TGTCTCAACAGTTGCCCCGTCTGCGCACT50.22810218978102187No Hit
TCCATAATCCATTAGTGACCAAGCCGCCAAT50.22810218978102187No Hit
TACAGGGTCAATTGTTGCGTAGTAATCTCC50.22810218978102187No Hit
TAGTCGGCTTCTTTCATAATTATTTGATGC40.18248175182481752No Hit
TTTGCTGGTAGATATGGGCACATCTGCACT40.18248175182481752No Hit
TCTGCACTGGTTGGATTGATAGGAACAGTC40.18248175182481752No Hit
TCTTCGTCCGACTCGACATCCATAATTGGC40.18248175182481752No Hit
TCGGCCCATAACATGGACATTTTCTTCATCTCCAGA40.18248175182481752No Hit
TCTTCACAGGGGCCGTCGGTCTGGGACACT40.18248175182481752No Hit
TACTTGCGCTTGCTGATCGGGAGTCAAA40.18248175182481752No Hit
TGAAGTCCCGAGTTATCGCTTTCTGTAAT40.18248175182481752No Hit
TACTTAATTGTGAACTCATGCCGGGGAATAACTCTTCGTCCGACTCGACA40.18248175182481752No Hit
AGTTTCTTCACAGGGGCCGTCGGTCTGGG40.18248175182481752No Hit
TGTAGATCGTCTGGAACTTGGTAAGAGA40.18248175182481752No Hit
TCGATGAAATAGTGGGGGCAGCAGACCT40.18248175182481752No Hit
TATGAGCTTCAACGGCGCAGCAGGGTGG40.18248175182481752No Hit
TGCACTTTGGCTCCGGATGCTTTCTC40.18248175182481752No Hit
TTCCTCCCATACTTGCGCTTGCTGATCGG40.18248175182481752No Hit
TATGGGCACATCTGCACTGGTTGGATT40.18248175182481752No Hit
TGTCTCAACAGTTGCCCCGTCTGCGCACTG40.18248175182481752No Hit
TGAGTACAGTTTGAAGAATTCTAGCACC40.18248175182481752No Hit
TTGGTCCAGTGAGGCGTTTGAAATATGAGC40.18248175182481752No Hit
TCACAGGGGCCGTCGGTCTGGGACACTCT40.18248175182481752No Hit
TCCGGGCACAGTTTCTTCACAGGGGACG40.18248175182481752No Hit
TGACCAAGCCGCCAATTCAATTGCTCTGTT40.18248175182481752No Hit
TTCGTCCGACTCGACATCCATAATTGGCG40.18248175182481752No Hit
GAGTAATTGTTCTCCCGTATTTCTGGGGC40.18248175182481752No Hit
TATGAGCTTCAACGGCGCAGCAGGGTG40.18248175182481752No Hit
CATACTTGCGCTTGCTGATCGGGAGTCAAATGCACTTGCACTTTGGCTCCGGATGCTTTCTCCGGGC40.18248175182481752No Hit
TGTTGTAGATCGTCTGGAACTTGGTAAGA40.18248175182481752No Hit
TTTGCTGGTAGATATGGGCACATCTGCACTG40.18248175182481752No Hit
TTGAAATATGAGCTTCAACGGCGCAGCAGGG30.13686131386861314No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTA30.13686131386861314No Hit
TCCGGGCACAGTTTCTTCACAGGGGACGT30.13686131386861314No Hit
TAGATATGGGCACATCTGCACTGGTTGG30.13686131386861314No Hit
TTCTGTAATTGTCTCAACAGTTGCCCCGT30.13686131386861314No Hit
TTAAATAACCTTCTGTTCATAAATTTTGGCAACTCTACCAATAACGGATTGGAGTGGTGGTAG30.13686131386861314No Hit
TGCTTCCAGTAGACTTCCTTCGGTTG30.13686131386861314No Hit
TTCACAGGGGCCGTCGGTCTGGGACACTCC30.13686131386861314No Hit
TCTGTTGTTGAGTACAGTTTGAAGAATTCTAGCACCACAATGAAATAAAGCAAACTTCGATTTGGTCCA30.13686131386861314No Hit
TCACAGGGGACGTCGGTCTGGGACACTCT30.13686131386861314No Hit
TAGAGATGGGCACATCTGCACTGGTTGGAT30.13686131386861314No Hit
TCTGGGACACTCTTGGAAGGTCAGCTTCCTCT30.13686131386861314No Hit
TAGATATGGGCACATCTGCACTGGTTG30.13686131386861314No Hit
TAGGAGGTGAACTCTCAAGGGATTCGTATGCATTCAAATCCAAATGAAATAGATTGTTTCGAACCAATCTACATA30.13686131386861314No Hit
TTCCTCTTCGCCTTCTTCATAGTTGTCGGA30.13686131386861314No Hit
TGAGTACAGTTTGAAGAATTCTAGCACCACAATGAA30.13686131386861314No Hit
TTCTTCACAGGGGCCGTCGGTCTGGGAC30.13686131386861314No Hit
TGCTCGATGAAATAGTGGGGGCAGCAGAC30.13686131386861314No Hit
AGTTTCTTCACAGGGGCCGTCGGTCTGG30.13686131386861314No Hit
TCGGTCTGGGACACTCTTGGAAGGTCA30.13686131386861314No Hit
TGATAACTTGATCCTCTTGAAATTTAACTCTAATGGAAC30.13686131386861314No Hit
TGCACTGGTTGGATTGATAGGAACAGTC30.13686131386861314No Hit
TTATCGCTTTCTGTAATTGTCTCAACAGTTGCCCCGTCTGCGCACTGGATGAAACCGTGCACAATGTCTTCCAAG30.13686131386861314No Hit
TCCAGATTCGCATGCTGACGCGACCACGCT30.13686131386861314No Hit
TCTGGGACACTCTTGGAAGGTCAGCTTCCT30.13686131386861314No Hit
TGTGGGGGCCTCGATCTTGTAATTTTATGT30.13686131386861314No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGA30.13686131386861314No Hit
ACAGTTTGAAGAATTCTAGCACCACAATG30.13686131386861314No Hit
TTAAGCACACAGTAAGTTTATAAAAAGACTTGAACAATATCCT30.13686131386861314No Hit
TGTTCTCCCGTATTTCTGGGGCGCTTAAACTGCCCATCTCGCCT30.13686131386861314No Hit
TCATCTCCAGATTCGCATGCTGACGCGACC30.13686131386861314No Hit
TTCTGAAATTGTCTCAACAGTTGCCCCGTC30.13686131386861314No Hit
TTCATCTCCAGATTCGCATGCTGACGCGACC30.13686131386861314No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCG30.13686131386861314No Hit
TGAAATATGAGTTTCAACGGCGCAGCAGG30.13686131386861314No Hit
TCATCGAGATCCCAAAACTAAAATTAAGCACACAGTAAGTTTATAAAAAGACTTGAACAATATCCT30.13686131386861314No Hit
CCCCGTCTGCGCACTGGATGAAACCGTGC30.13686131386861314No Hit
TACAGGGTCAATTGTTGCATAGTAATCTCC30.13686131386861314No Hit
TGTATAACGTGGACGTGGTCGTGGTGTT30.13686131386861314No Hit
TCGGACCCTTTACTACCTAATACATAGAAGT30.13686131386861314No Hit
AAACAGGTACCTACATCTAATTACAAACTAAACTAAATAACTAAACATTAAATCCTAAATAAATATTGCATCCAA30.13686131386861314No Hit
TTGGTTCTATATTCGGTTTTCCGGCAAACTATCTTGTTTCCAGGCTGTATTGGCTTGACT30.13686131386861314No Hit
TCATCTCCAGATTCGCATGCTGACGCGACCACGCTGCTGCTCGA30.13686131386861314No Hit
ACGGACCCTTTACTACCTAATACATAGAAGT30.13686131386861314No Hit
TTAGATTGATAATTCCCTGAGGATGTTCCTCCC30.13686131386861314No Hit
TATGGGCACATCTGCACTGGTTGGA30.13686131386861314No Hit
TACAATTCGAGCTTCATTACCAAATCGTGGGATCGAT30.13686131386861314No Hit
TAAGCACACAGTAAGTTTATAAAAAGACTTG30.13686131386861314No Hit
TGCTCTGTTGTTGAGTACAGTTTGAAGAATTCTAGCACCACGATGAAATA30.13686131386861314No Hit
TCAGCTCGTTGCAAGAACAGTACCGCATTGGC30.13686131386861314No Hit
TGTAAAACGTTTGCTTTGACAGGTATCCT30.13686131386861314No Hit
TCTGGGACACTCTTGGAAGGTCAGCTTCCTC30.13686131386861314No Hit
TACTTAATTGTGAACTCATGCCGGGGAATA30.13686131386861314No Hit
TTCGCATGCTGACGCGACCACGCTGCTGC30.13686131386861314No Hit
TGAAGTCCCGAGTTATCGCTTTCTGTAATTGTCTCAACAGTTGCCCCGTC30.13686131386861314No Hit
TTCCAGTAGACTTCCTTCGGTTGACCTACA30.13686131386861314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)