FastQCFastQC Report
Mon 30 Nov 2020
S33.raw.31032.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.31032.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94
Sequences flagged as poor quality0
Sequence length11-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGGTGTGGTTCACATTCAACATCGGCCCTCACAATTTAGCCACC33.1914893617021276No Hit
TTACCAAGCCGCGCCTTACCACCCTAGCTACCTGC33.1914893617021276No Hit
AAAAAGTAGCTCGGTGCGGGGTTCGAACCCG33.1914893617021276No Hit
AAAATCGGAAAAAAACTTGGCGTTTAGGTGTGGTTCACATTCAACATCGGCCCTCACAATTTAGCC22.127659574468085No Hit
AAGCTGCCCGTCCCGTACTTTGTAACAGA22.127659574468085No Hit
TAACGGAGAAAAAAAAGTAGCCCGGTGCGGG22.127659574468085No Hit
ACGGAGAAAAAAAAGTAGCCCGGTGCGGG22.127659574468085No Hit
TTACCAAGCCGCGCCTTACCACCCGAG22.127659574468085No Hit
TCGGAAAAAAACTTGGCGTTTAGGTGTGGT22.127659574468085No Hit
TGAAAGGGTTGGCAGTTGGAAATGTAAC22.127659574468085No Hit
TTGGCAGTTGGAAATGTAACGTCTGATTCGG22.127659574468085No Hit
GCATTTTGATTTGAACGCCCATTTTCATAATAAGTCTGGATAACTTTAACACGTTGTTGGACTGTGT22.127659574468085No Hit
TACCAAGCCGCGCCTTACCACCCGAGCTAC22.127659574468085No Hit
TCGGAAAAAAACTTGGCGTTTAGGTGTGG22.127659574468085No Hit
TACTTTGTAACGGAGAGAAAAAAAAGTAGCCCGGTGCGGGGTTCGAACCC22.127659574468085No Hit
TTGGAAATGTAACGTCTGATTCGGACTCATCTTACTCAATTCCTCAAATGACGTCTAGCGTCGGTCCT22.127659574468085No Hit
TACAAGCTGCCCGTCCCGTACTTTGTAACAG22.127659574468085No Hit
TGTTACCAAGCCGCGCCTTACCACCCTAGCAC22.127659574468085No Hit
TACAAGCTGCCCGTCCCGTACTTTGTAACG11.0638297872340425No Hit
AGCTGCCCGTCCCGTACTTTGTAACTGAGA11.0638297872340425No Hit
TTAGTGTTACCAAGCCGCGCCTTACCACCC11.0638297872340425No Hit
AAAAAACAAGCTGCCCGTCCCGTACTTT11.0638297872340425No Hit
CGAACCCGTGACCTTAGGGTTACCAAGCCGCGCCTTACCACCCGAG11.0638297872340425No Hit
CCTTAGGGTTACCAAGCCGCGCCTTACCACCCG11.0638297872340425No Hit
TGTCAAAAAAAAAATCGGAAAAAAACTTGGC11.0638297872340425No Hit
GGTTCTGGGCC11.0638297872340425No Hit
CATATGTATAACAAGCTGCCCGTCCCGTACTTTGTAACGGA11.0638297872340425No Hit
TAAACAAGCTGCCCGTCCCG11.0638297872340425No Hit
TTACCAAGCCGCGCCTTACCACCCTAG11.0638297872340425No Hit
CAAGCTGCCCGTCCCATACTTTGTAACGG11.0638297872340425No Hit
ACATTCAACATCGGCCCTCACAATTTAGCCACCCTTTATATCAGA11.0638297872340425No Hit
CAAGCCGCGCCTTACCACCCGA11.0638297872340425No Hit
TGTAACGGAGAGAAAAAAAAGTAGCCCGGTGCGGG11.0638297872340425No Hit
GTTACCAAGCCGCGCCTTACCACCCTAGCTACCT11.0638297872340425No Hit
TACAAGCTGCCCGTCCCGTACTTTGTAACAGA11.0638297872340425No Hit
AAAAGTAGCTCGGTGCGGGGTTCGAACCC11.0638297872340425No Hit
TACTGAACAAGCTGCCCGTCCCGTACTTTGTAACG11.0638297872340425No Hit
GTTTTCTTGGCTGTAC11.0638297872340425No Hit
TTCTACAGACCCGGTTTGCTCGAATTTTTCCACGATTTTTGCGATTGTACGCACATTTG11.0638297872340425No Hit
TTACAAGCTGCCCGTCCCGTACTTTGTAA11.0638297872340425No Hit
AAAAAAAAGTAGCTCGGTGCGGGGTTCGAAC11.0638297872340425No Hit
TAGACAAGCTGCCCGTCCCGTACTT11.0638297872340425No Hit
GCTGCCCGTCCCGTACTTTGTA11.0638297872340425No Hit
ACGCACATTTGGACGATGCATATCGTGCACGAAGTGTACGATATGCATTT11.0638297872340425No Hit
AACGTCTGATTCGGACTCATCTTACTCAATTCCTCAAATGACGTCTAGCGTCGGTCCTTGTGTACT11.0638297872340425No Hit
TTAACAGACCTCAGGGTTACCAAGCCGCGCCTTACCACCCA11.0638297872340425No Hit
TTTGGCAGTTGGAAATGTAACGTCTGATT11.0638297872340425No Hit
ACCAAGCCGCGCCTTACCACCCTAGCTACCTGCA11.0638297872340425No Hit
TTACCAAGCCGCGCCTTACCACCCTAGCTACCTG11.0638297872340425No Hit
TTGGTACTGAAAGGGTTGGCAGTTGGAAATGTAACGTCTGATTC11.0638297872340425No Hit
TACACAAGCTGCCCGTCCCGTACTTTGTAAC11.0638297872340425No Hit
ACCAAGCCGCGCCTTACCACCCTAGCTACCT11.0638297872340425No Hit
TTAAGGTTACCAAGCCGCGCCTTACCACCC11.0638297872340425No Hit
AACAAGCTGCCCGTCCCGTACTTTGT11.0638297872340425No Hit
TCCTCAAATGACGTCTAGCGTCGGTCCTTG11.0638297872340425No Hit
TACCAAGCCGCGCCTTACCACCCTCGCTAC11.0638297872340425No Hit
TACCAAGCCGCGCCTTACCACCCG11.0638297872340425No Hit
TTTGATTTGAACGCCCATTTTCATAATAAGTCTGGATAACTTTAACACGTTGTTGGATTGTGTATCTCTCT11.0638297872340425No Hit
ACCAAGCCGCGCCTTACCACCCTA11.0638297872340425No Hit
AAAAAAAATCGGAAAAAAACTTGGCGTTTAGGTGTGGT11.0638297872340425No Hit
TTAGCCACCCTTTATTACCCCATTGCTTGTGAGAGT11.0638297872340425No Hit
AAAAAAAGTAGCCCGGTGCGGG11.0638297872340425No Hit
TACCAAGCCGCGCCTTACCACCCGAGC11.0638297872340425No Hit
GACCCGGTTTGCTCGAAATTTTCCACGATTTTTGCGATTGTACGCACATTTGGACGATGCATATCGTGCACGAAG11.0638297872340425No Hit
AACGGAGAAAAAAAAGTAGCCCGGTGCGGG11.0638297872340425No Hit
ACAAGCTGCCCGTCCCG11.0638297872340425No Hit
CAAGCCGCGCCTTACCACCCTAGCTACCTGC11.0638297872340425No Hit
TAAAACAAGCTGCCCGTCCCGTACT11.0638297872340425No Hit
ATTCGGAAAAAAACTTGGCGTTTAGGTGTGGTTCACATTCAACATC11.0638297872340425No Hit
TGTTGCAGGTTGAACACAAAGAGATCCGAGTTTTCTTTAGCCAGTAGACT11.0638297872340425No Hit
AAAGTAGCCCGGTGCGGGGTTCGAACCCGTGACCTTAGGGTTACCAAGCCGCGCCTTACCACA11.0638297872340425No Hit
TGCTGATGGTGAACAATGACGGGTTCTGG11.0638297872340425No Hit
AGCTGCCCGTCCCGTACTTTGTAAC11.0638297872340425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)