FastQCFastQC Report
Mon 30 Nov 2020
S33.raw.3449.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.3449.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153
Sequences flagged as poor quality0
Sequence length11-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATGATGACA95.88235294117647No Hit
TGGACCATGAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAGGGC63.9215686274509802No Hit
TGGACCATGAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAA63.9215686274509802No Hit
GTATGGACCATGAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAGGGC63.9215686274509802No Hit
ATGGGAATAAAAACGACAGTCAAGGAGAGCACAAGGTTAGTTAGAACAGGAGGA42.6143790849673203No Hit
CATGAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCT42.6143790849673203No Hit
TGGACCATGAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCT42.6143790849673203No Hit
GAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCT42.6143790849673203No Hit
AAGGGCAGTAATGTTAAGGGGGGACGT31.9607843137254901No Hit
TCAAGGAGAGCACAAAGTTAGTTAGAACA31.9607843137254901No Hit
ACCGGAAGAACTCCTAAGGGCAGTAATGTTAAGGGGGGACGTCACATTGG31.9607843137254901No Hit
ATAAAAACGACAGTCAAGGAGAGCACAAGGTTAGTTAGAACAGGAGGACC31.9607843137254901No Hit
TAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAGGGC31.9607843137254901No Hit
GTAAGCTCCACGTAGAGAACACGTCATGTCACCTAGATGATCAACACATCAT31.9607843137254901No Hit
TTTGTTTTAAAATAGAACCCGGTATGGACCATGAGA21.3071895424836601No Hit
ATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAGGGCAGTAATGTTAAGGGGGGACGTCACAT21.3071895424836601No Hit
TGGACCATGAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAGGGCAGTAAT21.3071895424836601No Hit
TATTGGATGGCCTACCGGAAGAACTCCT21.3071895424836601No Hit
ATTATGATGAC21.3071895424836601No Hit
AATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCT21.3071895424836601No Hit
AAAAACGACAGTCAAGGAGAGCACAAGGTTAGTTAGAACAGGAGGACC21.3071895424836601No Hit
GTATGGACCATGAGATAATTAAGGAATATTTATTGGATGGCCTACCGGAA21.3071895424836601No Hit
CATGAGAGAATTAAGGAATATTTATTGGATGGCCT21.3071895424836601No Hit
AAGGGCAGTAATGTTAAGGGGGGACGTC21.3071895424836601No Hit
AGGTGTAAGCTCCACGTAGAGAACACGTCATGTCACCTAGATG21.3071895424836601No Hit
CATGAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAGGACAGTAATGTTAAGGGG21.3071895424836601No Hit
GAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTT21.3071895424836601No Hit
GAACCCGGTATGGACCATGAGAGAATTAAGGAATATTTATTGGATGGCCT21.3071895424836601No Hit
ACCCTTTGTTTTAAAATAGAACCCGGTATGGACCATG21.3071895424836601No Hit
CAAGATATACAAACCCTTTGTTTTAAAATAGAACCCGGTATGGACC21.3071895424836601No Hit
GAGAGAATTAAGGAATATTTATTGGATGGTCTACCGGAAGAACTCCTAAGGGC21.3071895424836601No Hit
TGGACCATGAGATAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAGGGCAGTAATGTA21.3071895424836601No Hit
ATATACAAACCCTTTGTTTTAAAATAGAACCCGGTATGGACCATGAGAGA21.3071895424836601No Hit
GAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAGGGCAGTAATGTTAAGGGGGG10.6535947712418301No Hit
GCTCCACGTAGACAACACGTCATGTCACC10.6535947712418301No Hit
AATATTTATTGGATGGCCTACCGGAAGAACTCCTA10.6535947712418301No Hit
AAAAACGACAGTCAAGGAGAACACAAAGTTAGTTAGAACAGGAGGA10.6535947712418301No Hit
CCCTTTGTTTTAAAATAGAACCCGGTATGGACCATGAGAG10.6535947712418301No Hit
AAGGGCAGTAATGTTAAGGGGGGACGTCACATTGGACGACGTCATAACAAATATAAAGAAAATCAAT10.6535947712418301No Hit
CATGAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAGGAC10.6535947712418301No Hit
ATAGAACCCGGTATGGACCATGAGAGAATTAAGGAATATTTATTGGATGGCCT10.6535947712418301No Hit
GTATGGACCATGAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAAGGC10.6535947712418301No Hit
ATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAGGGCAGTAATGTTAAGGGGGGACGT10.6535947712418301No Hit
AGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAGGGCAGTAATGTTAAGGGG10.6535947712418301No Hit
TCCACGTAGAGAACACGTCATGTCACCTAGATGATCAACACATCATCTAGTGATAACCACCCTAATCGGTAACGA10.6535947712418301No Hit
CGACAGTCAAGGAGAACACAAAGTTAGTTAGAACAGGAGGAC10.6535947712418301No Hit
TTCAAGATATACAAACCCTTTGTTTTAAAATAGAACCCGGTATGGACCATGAGA10.6535947712418301No Hit
TGGGAATAAAAACGACAGTCAAGGAGAGCACAAGGTTAGTTAGAACAGGAGGA10.6535947712418301No Hit
ATGGCCTACCGGAAGAACTCCT10.6535947712418301No Hit
AAGATATACAAACCCTTTGTTTTAAAATAGAACCCGGTATGGACCATGAGAG10.6535947712418301No Hit
TGGACCATGAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAGGG10.6535947712418301No Hit
AAGGAGAGCACAAGGTTAGTTAGAACAGGAGGACC10.6535947712418301No Hit
CCATGAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCT10.6535947712418301No Hit
TTAAGGAATATTTATTGGATGGCCTACCGGAAGAA10.6535947712418301No Hit
CAAGATATACAAACCCTTTGTTTTAAAATAGAACCCGGTATGGACCATGAGAGA10.6535947712418301No Hit
AGGAATATTTATTGGATGGCCTACCGGAAGAACTCCT10.6535947712418301No Hit
TAGAACCCGGTATGGACCATGAGAGAATTAAGGAATATTTATTGGAAGGCCT10.6535947712418301No Hit
GAGAATTAAGGAATATTTATTGGATGGCCTACCGGAA10.6535947712418301No Hit
CAAACCCTTTGTTTTAAAATAGAACCCGGTATGGACCATGAGA10.6535947712418301No Hit
GTAACGATAAGATGGGAATAAAAACGACAGTCAAGGAGAGCACAAGGTTAGTTAGAACAGGAGGACC10.6535947712418301No Hit
AGCTCCACGTAGAGAACACGTCATGTCACCTAGATGATCAACACATCATC10.6535947712418301No Hit
TAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCC10.6535947712418301No Hit
ACGACAGTCAAGGAGAGCACAAGGTTAGTTAGAACAGGAGGACC10.6535947712418301No Hit
ACGTAGACAACACGTCATGTCACCTAGATGATCAACACATCATCTAGTGA10.6535947712418301No Hit
CCTAAGGGCAGTAATGTTAAGGGGGGACGTCACATTGGA10.6535947712418301No Hit
ACAATCGCAGGAAAAGAGTGTGAACTAAA10.6535947712418301No Hit
ATTCAAGATATACAAACCCTTTGTTTTAAAATAGAACCCGGTATGGACCATGAGAGAATTAA10.6535947712418301No Hit
GAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAAGG10.6535947712418301No Hit
ATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAGG10.6535947712418301No Hit
CATGAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAGGGCAGTAATGTTAAGGGG10.6535947712418301No Hit
AAGCTCCACGTAGAGAACACGTCATGTCACCTAGATG10.6535947712418301No Hit
GACCATGAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAGGAC10.6535947712418301No Hit
AAGCTCCACGTAGACAACACGTCATGTCACCTAGATGATCAACACATCAT10.6535947712418301No Hit
AGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCT10.6535947712418301No Hit
AAGGACGAGAA10.6535947712418301No Hit
AGGGCAGTAATGTTAAGGGGGGACGTCACATTGGACGATGTCATATTT10.6535947712418301No Hit
CCCTTTGTTTTAAAATAGAACCCGGTATGGACCATGAGA10.6535947712418301No Hit
AATAAAAACGACAGTCAAGGAGAGCACAAGGTTAGTTAGAACAGG10.6535947712418301No Hit
CTTTGTTTTAAAATAGAACCCGGTATGGACCATGAGAGAATTAAGGAATA10.6535947712418301No Hit
GAAGAACTCCTAAGGGC10.6535947712418301No Hit
GAGAACACGTCATGTCACCTAGATGATCAACACATCATCTAGTGATAACC10.6535947712418301No Hit
ACGACAGTCAAGGAGAGCACAAGGTTAGTTAGAACAGGAGGACCT10.6535947712418301No Hit
CTTTGTTTTAAAATAGAACCCGGTATGGACCATGAGAGAATTAAGGAATATTTATTGGATGGCCT10.6535947712418301No Hit
GACCATGAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTC10.6535947712418301No Hit
TGGACCATGAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAGGGCA10.6535947712418301No Hit
GACCATGAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCT10.6535947712418301No Hit
CTACCGGAAGAACTCC10.6535947712418301No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)