FastQCFastQC Report
Mon 30 Nov 2020
S33.raw.4554.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.4554.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093
Sequences flagged as poor quality0
Sequence length12-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCTCGGTACTGACTCGACAAGCGCC12511.436413540713632No Hit
TGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAA585.3064958828911255No Hit
TCTATATTGTCGAATAACCCACCTCTTGG504.574565416285453No Hit
TTGGGCTCGGTACTGACTCGACAAGCGCCT433.9341262580054894No Hit
TTAACGACCCGCTCTGTTCCATATCGATA333.019213174748399No Hit
TTTGTTAACTGGCCGACTTCCCCCGCCT312.8362305580969807No Hit
TATTGTCGAATAACCCACCTCTTGG292.6532479414455628No Hit
TAACGACCCGCTCTGTTCCATATCGATAT282.5617566331198534No Hit
CACGTCGTGTACGACGCGAGACCGCGGAG201.8298261665141813No Hit
TTAACGACCCGCTCTGTTCCATATCGATAT191.738334858188472No Hit
TCGGTACTGACTCGACAAGCGCCTCGTAC181.646843549862763No Hit
TATACGTCGTGTACGACGCGAGACCGCGGA181.646843549862763No Hit
TGGGCTCGGTACTGACTCGACAAGCGCCT171.555352241537054No Hit
TAAAAGGACCGATTCGGGCAGTGAGTGTC161.463860933211345No Hit
TAACACAAGCCCGATCGCTCCGATACATG151.3723696248856359No Hit
TGAATTGCCGTGGCTCGGAAGTTGGGCTC141.2808783165599267No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGAC131.1893870082342177No Hit
TGAAAGTGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAA131.1893870082342177No Hit
TCGGTACTGACTCGACAAGCGCCTCGTACT131.1893870082342177No Hit
AAAAGTCACCGTCGTCCTCATGACTGCCT100.9149130832570906No Hit
TGGGCTCGGTACTGACTCGACAAGCGCC90.8234217749313815No Hit
TACAATTTCTGCATCGTTATAGCACTGAT80.7319304666056725No Hit
TCTACATTGTCGAATAACCCACCTCTTGGT80.7319304666056725No Hit
TACTGACTCGACAAGCGCCTCGTACTTGT70.6404391582799633No Hit
TATTGTCGAATAACCCACCTCTTGGTG70.6404391582799633No Hit
TCAAAACTCCCTCTTCGAACGTAACACAAG60.5489478499542544No Hit
TCTATATTGTCGAATAACCCACCTCTTGGT60.5489478499542544No Hit
AAGTGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAA50.4574565416285453No Hit
TGCCGAAGCCATAAAAGGACCGATTCGGGC50.4574565416285453No Hit
TATTGTCGAATAACCCACCTCTTGGT50.4574565416285453No Hit
TAGGGAATTTTTGAATGGCTGCTATTTT50.4574565416285453No Hit
GTGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAA50.4574565416285453No Hit
CACGACGCATTTTCGTAGTTCTCCCCAAGC50.4574565416285453No Hit
TCTGAGAAAAAGTCACCGTCGTCCTCATGAC40.36596523330283626No Hit
TTCCAACGTCTCGTTCCATCGGATC40.36596523330283626No Hit
TAGACGTTAACGGCGCTGAGGGGTGTTG40.36596523330283626No Hit
CGCGAGACCGCGGAGGCACTCTTTGAAGG40.36596523330283626No Hit
TTGGGCTCGGTACTGACTCGACAAGCGCCC40.36596523330283626No Hit
TCCCAAACTTCGACTCGGACCAGTGCCGG40.36596523330283626No Hit
TATTTTTTTTGGGCGGATTCTTTCGGGG40.36596523330283626No Hit
TGAATTGCCGTGGCTCGGAAGTTGGGCTCT40.36596523330283626No Hit
TCCGAATCGATATTGTCGAATAACCCACCT40.36596523330283626No Hit
TTTGTTAACTGGCCGACTTCCCCCGCCTGA30.2744739249771272No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGCC30.2744739249771272No Hit
ATCTATATTGTCGAATAACCCACCTCTTG30.2744739249771272No Hit
TTGAATTGCCGTGGCTCGGAAGTTGGGCTC30.2744739249771272No Hit
TCGAGAAATTGGGACACTGTAATATTGGGT30.2744739249771272No Hit
TACACGTCGTGTACGACGCGAGACCGCGGA30.2744739249771272No Hit
TTTACGGTAGTAGCCTAGTAATCCGAGAAA30.2744739249771272No Hit
TGAGGACCAGGGGCTGTGTGAGGGCCTAA30.2744739249771272No Hit
TTGGGGATCCCGTAAGAAATACACGTCGTG30.2744739249771272No Hit
TTGGGCTCGGTACTGACTCGACAAGCGCCTCGTACTTGTCGATTAAC30.2744739249771272No Hit
TTGATTCCTCATTGACTTCAATTTGATGAAAGCCTGACGCAAGGTCTAGG30.2744739249771272No Hit
TATATTGTCGAATAACCCACCTCTTGG30.2744739249771272No Hit
TACTGACTCGACAAGCGCCTCGTACT30.2744739249771272No Hit
TAACGACCCGCTCTGTTCCATATCGATATT30.2744739249771272No Hit
TTTTCGCCGCTGCAGCCGAATACGGTAGGTACGCCCTCCGATTTGAACGTACATAACTTTTT30.2744739249771272No Hit
TTTGTCCGGAGGCATCTATTTTTTTGGGA30.2744739249771272No Hit
TTAACGACCCGCTCTGTTCCATATCGATATTGTCGAATAACCCACCTCTTGGTG30.2744739249771272No Hit
TAACGACCCGCTCTGTTCCATATCGATA30.2744739249771272No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGC30.2744739249771272No Hit
CCCCGCCTGAAAGTGGTCCATTAAGGCCTTGTTGTAGACC30.2744739249771272No Hit
TTACGGTAGTAGCCTAGTAATCCGAGAAATGATTTCATCTGTGTTTGTGTTCGTGGAATAGGGAAT30.2744739249771272No Hit
TAACAGGAGTACGGCCCACAGTAGCGTC30.2744739249771272No Hit
TTGTTAACTGGCCGACTTCCCCCGCCT30.2744739249771272No Hit
TCGGTAGTTAATTCGAAGGGTTTCGAGAA30.2744739249771272No Hit
TGATGGCAGGAGATTGAACGTTTGTTTCG30.2744739249771272No Hit
TACTGGAAGTGCGGAGAACTTTAGTCCCA30.2744739249771272No Hit
TGCAATATAGACGTTAACGGCGCTGAGGG20.18298261665141813No Hit
TCGATTAACGAACTCCACACTTCTTTGGG20.18298261665141813No Hit
GGATGAAGACGCAGCGGCATGTTGAACTG20.18298261665141813No Hit
TTGGGCGGATTCTTTCGGGGGTGACAATA20.18298261665141813No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGA20.18298261665141813No Hit
TCGATATTGTCGAATAACCCACCTCTTGG20.18298261665141813No Hit
TTAGAGTTTCAAATGCTGTTGGTTCTGCT20.18298261665141813No Hit
TACATTGTCGAATAACCCACCTCTTGGT20.18298261665141813No Hit
ACGTCTTGCTTTCGACGCGCTAGAGTGGCTGCATGAAGGAAGATTGATGGCAGGAGATTGAACGTTTGTTTCGG20.18298261665141813No Hit
TTAACGACCCGCTCTGTTCCATATCGATAA20.18298261665141813No Hit
TCTTGCTTTCGACGCGCTAGAGTGGCTGC20.18298261665141813No Hit
TTCGGTGTCCACACGAGAAACAGAAACGC20.18298261665141813No Hit
ACCACCCAAAGAGATGAGGACCAGGGGCTGT20.18298261665141813No Hit
TAAGAAATACACGTCGTGTACGACGCGAGACCGCGGAGGCACTC20.18298261665141813No Hit
TCAAAACTCCCTCTTCGAACGTAACACAAGC20.18298261665141813No Hit
TCCCCAGGGGAGCGGCGAATATTAAGTTTTCGTGGTTAAG20.18298261665141813No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGC20.18298261665141813No Hit
TAAAAGGACCGATTCGGGCAGTGAGTGTCT20.18298261665141813No Hit
TCGTGTACGACGCGAGACCGCGGAGGCACTCTTTGAAGGAGTGCCAC20.18298261665141813No Hit
TCGTGGATGACGTGGACGTGGTCGTTGTG20.18298261665141813No Hit
TTAGACAGAATAATAACAGGAGTACGGCC20.18298261665141813No Hit
TGCCTCCTGCCACCAATCCTTCTTGTTTT20.18298261665141813No Hit
TGCCACGACGCATTTTCGTAGTTCTCCCC20.18298261665141813No Hit
CATTGTCGAATAACCCACCTCTTGGT20.18298261665141813No Hit
TCCGGCGCGCTAGAGTGGCTGCATGAAGGAA20.18298261665141813No Hit
TCAAAACTCCCTCTTCGAACGTAACACAAGCCCGATCGCTCCGATACATG20.18298261665141813No Hit
TATAGACGTTAACGGCGCTGAGGGGTGTT20.18298261665141813No Hit
TCAAAACTCCTCTCGATTTGCGGGGAGGAGGACTCAAAACTCCCTCTTCGAACGTAACACAAGCCCGATCGCT20.18298261665141813No Hit
TTAACGACCCGCTCTGTTCCATATCGATATT20.18298261665141813No Hit
TATAGACGTTAACGGCGCTGAGGGGTGT20.18298261665141813No Hit
TATACGTCGTGTACGACGCGAGACCGCGGAC20.18298261665141813No Hit
TATACGTCGTGTACGACGCGAGACCGCGGAA20.18298261665141813No Hit
TTTTGTTAACTGGCCGACTTCCCCCGCCT20.18298261665141813No Hit
TCATGACTGCCTCCTGCCACCAATCCTTC20.18298261665141813No Hit
TATTAATGAGACCGCGGAGGACTATCTC20.18298261665141813No Hit
TTCGACTGTTAGGTGGGCAAAATTTGGGA20.18298261665141813No Hit
CGGAAATTGGGCTCGGTACTGACTCGACAAGCGCCTCGTACTTGTCGATTA20.18298261665141813No Hit
TTCAAATGCTGTTGGTTCTGCTTGCGGCTC20.18298261665141813No Hit
TTAACGACCCGCTCTGTTCCATATCGAT20.18298261665141813No Hit
AACGACCCGCTCTGTTCCATATCGATATTGTC20.18298261665141813No Hit
TGCGGCTCATTCCAAAGAAGAATTCTTTT20.18298261665141813No Hit
TCCTCATGACTGCCTCCTGCCACCAATCC20.18298261665141813No Hit
TGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCA20.18298261665141813No Hit
TATCGCCTATTGTTTTTTCGTTTAGTTTC20.18298261665141813No Hit
AAAGTGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAA20.18298261665141813No Hit
TACACGTCGTGTACGACGCGAGACCGCGGAGGCACTCTT20.18298261665141813No Hit
CTCCCTCTTCGAACGTAACACAAGCCCGATC20.18298261665141813No Hit
TTACGGTAGTAGCCTAGTAATCCGAGAAAT20.18298261665141813No Hit
TCTTGCTTTCGACGCGCTAGAGTGGCTG20.18298261665141813No Hit
TCGGATCCTATTGCATATGCGGATGCATC20.18298261665141813No Hit
TGCGAGACCGCGGAGGCACTCTTTGAAGCAG20.18298261665141813No Hit
TCGTACTTGTCGATTAACGAACTCCACACT20.18298261665141813No Hit
TTATTAATGAGACCGCGGAGGACTATCTC20.18298261665141813No Hit
TTCGAGAAATTGGGACACTGTAATATTGGG20.18298261665141813No Hit
TCGTCCTCATGACTGCCTCCTGCCACCAATC20.18298261665141813No Hit
TATATTGTCGAATAACCCACCTCTTGGT20.18298261665141813No Hit
TTAATTCGAAGGGTTTCGAGAAATTGGGAC20.18298261665141813No Hit
TCCCTCTTCGAACGTAACACAAGCCCGATC20.18298261665141813No Hit
TAACACAAGCCCGATCGCTCCGATACATGG20.18298261665141813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)