FastQCFastQC Report
Mon 30 Nov 2020
S33.raw.5721.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.5721.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length12-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCATCGTCCAAATGTGCTTACAATCGCAAAAATCGTGGAAAATTCGAGCAAACCGGGTCTGTAGA311.11111111111111No Hit
CAGTGCTGTAGC311.11111111111111No Hit
ATTGTGAGGGCCGATGTTGAATGTGAACCACACCTAAACGCCAAGTT27.4074074074074066No Hit
TTTAACCATAGAGAGATACACAGTCCAACAACGTGTTAAAGTTATCCAGACTTATTATGAAAATGGGCGTTCAAA27.4074074074074066No Hit
GTGAACCTTTACT27.4074074074074066No Hit
GCAAAAATCGTGGAAAAATTCGAGCAAACCGGGTCTGTAGA13.7037037037037033No Hit
TTTATTTTTATT13.7037037037037033No Hit
ACTTCGTGCACGATATGCATCGTCCAAATGTGCGTACAATCGCAAAAATC13.7037037037037033No Hit
CGTGCACGATATGCATCGTCCAAATGTGCTTACAATCGCAAAAATCGTGGAAAATTCGAGCAAACCGGGTCT13.7037037037037033No Hit
CCCGTCCGGCGCA13.7037037037037033No Hit
ATGCGGACATTC13.7037037037037033No Hit
CATCGTCCAAATGTGCGTACAATCGCAAAAATCGTGGAAAAATTCGAGCAAACCGGGTCTGTAGAAG13.7037037037037033No Hit
TAAATTGTGAGGGCCGATGTTGAATGTGAACCACACCT13.7037037037037033No Hit
ATTCGAGCAAACCGGGTCTGTAGAAGATG13.7037037037037033No Hit
AAAATTCGAGCAAACCGGGTCTGTAGAAG13.7037037037037033No Hit
ATGCATCGTCCAAATGTGCGTACAATCGCAAAAATCGTGAAAAAATTCGAGCAAACCGGGTCTGTAGAAGATG13.7037037037037033No Hit
GAGCAAACCGGGTCTGTAGAA13.7037037037037033No Hit
TCGTGCACGATATGCATCGTCCAAATGTGCGTACAATCGCAAAAATCGTGGAAAAATTCGAGCAAACCGGGTCTG13.7037037037037033No Hit
ATCGCAAAAATCGTGGAAAAATTCGAGCAAACCGGGTCTGTAGA13.7037037037037033No Hit
TTCGTGCACGATATGCATCGTCCAAATGTGCGTACAATCGCAAAAATCGTGGAAAAATTCGAGCAAACCGGGTC13.7037037037037033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)