FastQCFastQC Report
Mon 30 Nov 2020
S34.15-35nt.12121.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.12121.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130
Sequences flagged as poor quality0
Sequence length15-33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTAACACAAGAATAAGAGACAAGCCT7154.61538461538461No Hit
ATAATATTTACTGTCAATAAAGAGTT43.076923076923077No Hit
TTGTATTCGCTTCCGTGTCTGTTCGTTC43.076923076923077No Hit
TACTAACACAAGAATAAGAGACAAGCCTCCAC32.307692307692308No Hit
AAGGAAAGGGACGGGCTATTGTATTCGCTT32.307692307692308No Hit
TTTACTGTCAATAAAGA21.5384615384615385No Hit
AAGGAAAGGGACGGGCT21.5384615384615385No Hit
TAACACAAGAATAAGAGACAAGCCTCCAC21.5384615384615385No Hit
TGACTCCACCACAAGAAGAAGAGACAAGC21.5384615384615385No Hit
TATACTAACACAAGAATAAGAGACAAGC21.5384615384615385No Hit
CTTTGATTGTATAATATTTACTGTC21.5384615384615385No Hit
AACACAAGAATAAGAGACAAGCCTCCACC21.5384615384615385No Hit
ACTAACACAAGAATAAGAGACAAGCCT21.5384615384615385No Hit
TTAGCCCGTCCCTATTTATAACTTCAAGGA21.5384615384615385No Hit
AAAGAAAGGAATGACAACGCCGGCGCC10.7692307692307693No Hit
TATACTAACACAAGAATAAGAGACAAGCC10.7692307692307693No Hit
TACTAACACAAGAATAAGAGACAAGCCA10.7692307692307693No Hit
TTTCGCCCTTTGATTGTATAATATTTACTGT10.7692307692307693No Hit
ACAAGAAGAAGAGACAAGCCCTAACCCT10.7692307692307693No Hit
ATAAGGAAAGGGACGG10.7692307692307693No Hit
AAAAAGGAAAGGGACGGGCTA10.7692307692307693No Hit
GTGACGGGCTATTGTATTCGCTTC10.7692307692307693No Hit
AAAAGGAAAGGGACGGGCT10.7692307692307693No Hit
TGACTCCACCACAAGAAGAAGAGACAAG10.7692307692307693No Hit
AAAGGAAAGGGACGGGCTATTGTATTCGCTT10.7692307692307693No Hit
TGCTTTTTAGCCCGTCCCTATTTATAACTT10.7692307692307693No Hit
TAATGTGTTTGTCCTAGTCCACC10.7692307692307693No Hit
ATAAAAAGGAAAGGGACGGGCT10.7692307692307693No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCCCT10.7692307692307693No Hit
TAAAAAGGAAAGGGACGGGCTATTGTATT10.7692307692307693No Hit
TACTAACACAAGAATAAGAGACAAGCCTCCACC10.7692307692307693No Hit
ACAAGAAGAAGAGACAAGC10.7692307692307693No Hit
AGTTATAAAAAGGAAAGGGACGGGCTATT10.7692307692307693No Hit
TCGCCCTTTGATTGTATAATATTTACTGTC10.7692307692307693No Hit
TTCGCCCTTTGATTGTATAATATTTACTGT10.7692307692307693No Hit
TATTAGATCAAAACC10.7692307692307693No Hit
ATTGTATAATATTTACTG10.7692307692307693No Hit
AAGGGTTGAATGGTAGAGATTGCCTAAGGCAAT10.7692307692307693No Hit
TACTAACACAAGAATAAGAGACAAGCCTCCA10.7692307692307693No Hit
AAGTTATAAAAAGGAAAGGGACGGGCTATTGT10.7692307692307693No Hit
TACTAACACAAGAATAAGAGACAAGCC10.7692307692307693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)