FastQCFastQC Report
Mon 30 Nov 2020
S34.15-35nt.1532.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.1532.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099
Sequences flagged as poor quality0
Sequence length15-35
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGGTAGCTCGGGTGGTAAGGCGCGGC23711.291090995712244No Hit
TGTAGGTAGCTCGGGTGGTAAGGCGCGG1537.289185326345879No Hit
TAGGTAGCTCGGGTGGTAAGGCGCGGCT1004.76417341591234No Hit
TGTAGGTAGCTCGGGTGGTAAGGCGCGGCT633.001429252024774No Hit
TGTAGGTAGCTCGGGTGGTAAGGCGCG422.0009528346831824No Hit
TGCAGGTAGCTCGGGTGGTAAGGCGCGGC411.953311100524059No Hit
TGCAGGTAGCTCGGGTGGTAAGGCGCGG351.6674606955693185No Hit
TAGCTCGGGTGGTAAGGCGCGGCTTGGTAACC301.4292520247737017No Hit
TCGGGTGGTAAGGCGCGGCTTGGTAACC251.191043353978085No Hit
CTCGGGTGGTAAGGCGCGGCTTGGTAACC221.0481181515007145No Hit
TTACAAAGTACGGGACGGGCAGCTTGTT221.0481181515007145No Hit
TGCAGGTAGCTCGGGTGGTAAGGCGCGGCT211.0004764173415912No Hit
TGCAGGTAGCTAGGGTGGTAAGGCGCGGC200.9528346831824679No Hit
TGTAGGTAGCTCGGGTGGTAAGGCGC160.7622677465459743No Hit
TAGGTAGCTCGGGTGGTAAGGCGCGGC160.7622677465459743No Hit
TCACGGGTTCGAACCCCGCACCGGGCT160.7622677465459743No Hit
CACGGGTTCGAACCCCGCACCGGGC160.7622677465459743No Hit
GTAGCTCGGGTGGTAAGGCGCGGCTTGGTAACCCT150.7146260123868509No Hit
CACGGGTTCGAACCCCGCACCGGGCTAC140.6669842782277274No Hit
TGTAGGTAGCTCGGGTGGTAAGGCG140.6669842782277274No Hit
GTAGGTAGCTCGGGTGGTAAGGCGCGGCT130.6193425440686041No Hit
TAGGTAGCTCGGGTGGTAAGGCGCGG130.6193425440686041No Hit
TAAGGCGCGGCTTGGTAACCC130.6193425440686041No Hit
AGATGTGTAGGTAGCTCGGGTGGTAAGGCGCGG130.6193425440686041No Hit
CACGGGTTCGAACCCCGCACCGGGCT130.6193425440686041No Hit
TAGGTAGCTCGGGTGGTAAGGCGCGGCTTGGTA120.5717008099094807No Hit
AGGTAGCTCGGGTGGTAAGGCGCGGCTTGGTAACC120.5717008099094807No Hit
TCATGAGCTCTAGGATACCTACTCCAAGT120.5717008099094807No Hit
TAGCTCGGGTGGTAAGGCGCGGCTTGGTAACCC110.5240590757503573No Hit
TGCAGGTAGCTCGGGTGGTAAGGCGCG110.5240590757503573No Hit
GTAGGTAGCTCGGGTGGTAAGGCGCGGC110.5240590757503573No Hit
TGCAGGTAGCTCGGGTGGTAAGGCGC110.5240590757503573No Hit
TCACGGGTTCGAACCCCGCACCGGGC100.47641734159123394No Hit
GTAGCTCGGGTGGTAAGGCGCGGCTTGGTAACCC100.47641734159123394No Hit
TGTAGGTAGCTCGGGTGGTAA90.4287756074321105No Hit
CACGGGTTCGAACCCCGCACCGGGCTACT90.4287756074321105No Hit
TCACGGGTTCGAACCCCGCACCGGGCTACTT90.4287756074321105No Hit
TAAGGCGCGGCTTGGTAACCCTAAGGTCAC90.4287756074321105No Hit
TGTAGGTAGCTCGGGTGGTAAGG80.38113387327298714No Hit
AGCTCGGGTGGTAAGGCGCGGCTTGGTAACC80.38113387327298714No Hit
TATGTAGCTCGGGTGGTAAGGCGCGGCT80.38113387327298714No Hit
AGCTCGGGTGGTAAGGCGCGGCTTGGTAACCC80.38113387327298714No Hit
GTAGGTAGCTCGGGTGGTAAGGCGCGG80.38113387327298714No Hit
GGTAGCTCGGGTGGTAAGGCGCGGCTTGGTAACC80.38113387327298714No Hit
CGTTACAAAGTACGGGACGGGCAGCTTG70.3334921391138637No Hit
TCACGGGTTCGAACCCCGCACCGGGCTACT70.3334921391138637No Hit
TCGGGTGGTAAGGCGCGGCTTGGTAACCC70.3334921391138637No Hit
TAGGTAGCTCGGGTGGTAAGGCGCGGCTT70.3334921391138637No Hit
CAGGTAGCTCGGGTGGTAAGGCGCGGCT70.3334921391138637No Hit
TCAGATGAGACAGCGGGAAAGGTACAAGT70.3334921391138637No Hit
TAAGGCGCGGCTTGGTAACCCTAAGGTACG60.28585040495474034No Hit
CAAAGTACGGGACGGGCAGCTT60.28585040495474034No Hit
TACAGATGTGCAGGTAGCTAGGGTGGTA60.28585040495474034No Hit
TGTAGGTAGCTCGGGTGGTAAGGCGCGGCTTGGT60.28585040495474034No Hit
TAGGTAGCTCGGGTGGTAAGGCGCGGCTTGGTAAC60.28585040495474034No Hit
TCACGGGTTCGAACCCCGCACCGGGCTAC60.28585040495474034No Hit
TGCAGGTAGCTAGGGTGGTAAGGCGC60.28585040495474034No Hit
TACAAGCTGCCCGTCCCGTACTTTGTAACG60.28585040495474034No Hit
TAGCTCGGGTGGTAAGGCGCGGCTTGGTA60.28585040495474034No Hit
CGCACCGGGCTACTTTTT50.23820867079561697No Hit
TAGGTAGCTCGGGTGGTAAGGCGCGGCTTG50.23820867079561697No Hit
GGTAGCTCGGGTGGTAAGGCGCGGCTTGGTAACCC50.23820867079561697No Hit
CGTTACAAAGTACGGGACGGGCAGCTT50.23820867079561697No Hit
TGCAGGTAGCTAGGGTGGTAAGGCGCGG50.23820867079561697No Hit
TCCTTGCCCGAACCCAGGAAAATTGTCAAAAC50.23820867079561697No Hit
TGCAGGTAGCTCGGGTGGTAAGGCGCGGT50.23820867079561697No Hit
TAGGTAGCTCGGGTGGTAAGGC50.23820867079561697No Hit
CGTTACAAAGTACGGGACGGGCAG50.23820867079561697No Hit
GTAGCTCGGGTGGTAAGGCGCGGCTTGGTAACC50.23820867079561697No Hit
ACGGGTTCGAACCCCGCACCGGGCTACTT50.23820867079561697No Hit
GTAGGTAGCTCGGGTGGTAAGGCGC50.23820867079561697No Hit
TGTAGCTCGGGTGGTAAGGCGCGGCTTGG50.23820867079561697No Hit
TAGGTAGCTCGGGTGGTAAGGCG50.23820867079561697No Hit
TCGGGTGGTAAGGCGCGGCTTGGTA40.19056693663649357No Hit
CACGGGTTCGAACCCCGCACCGGGCTACTT40.19056693663649357No Hit
TCGGGTGGTAGGGCGCGGCTTGGTAACCC40.19056693663649357No Hit
TGAAGGTAGCTCGGGTGGTAAGGCGCGGC40.19056693663649357No Hit
CTCGGGTGGTAAGGCGCGGCT40.19056693663649357No Hit
TAACCCTAAGGTCACGGGTTCGAACCC40.19056693663649357No Hit
TGTAGGTAGCTCGGGTGGTAAG40.19056693663649357No Hit
TGCAGGTAGCTCGGGTGGTAAGGCGCGGA40.19056693663649357No Hit
TGCAGGTAGCTCGGGTGGTAAGGCG40.19056693663649357No Hit
TTCAGATGAGACAGCGGGAAAGGTACAAGT40.19056693663649357No Hit
AGGTAGCTCGGGTGGTAAGGCGCGGCT40.19056693663649357No Hit
CAAGTATCAGCACAGTATTTTGGCCAGCAACTC40.19056693663649357No Hit
TGGTAAGGCGCGGCTTGGTAACCCTAAGGT40.19056693663649357No Hit
GCACAGTATTTTGGCCAGCAACTC40.19056693663649357No Hit
GTAAGGCGCGGCTTGGTAACCCT40.19056693663649357No Hit
ACGGGTTCGAACCCCGCACCGGGCTACT40.19056693663649357No Hit
GCTCGGGTGGTAAGGCGCGGCTTGGTAACC40.19056693663649357No Hit
TAGGTAGCTCGGGTGGTAAGGCGCGGCTTGG40.19056693663649357No Hit
TGTAGCTCGGGTGGTAAGGCGCGGCTTGGTC40.19056693663649357No Hit
ACGGGTTCGAACCCCGCACCGGGCTAC40.19056693663649357No Hit
TACAGGTAGCTCGGGTGGTAAGGCGCGG40.19056693663649357No Hit
TCACTTTCGATTTCTTTCCCGTGCTCCGT40.19056693663649357No Hit
TGTTTGAGGCAAACGGTAGGAGATAGCGC30.14292520247737017No Hit
TACAGATGTGTAGGTAGCTCGGGTGGTA30.14292520247737017No Hit
TGTAGGTAGCTCGGGTGGTAAGGCGCGGCTTGGTA30.14292520247737017No Hit
TGCAGGTAGCTCGGGTGGTAAGGCGCGGCTAAA30.14292520247737017No Hit
CCGTTACAAAGTACGGGACGGGCAGCTT30.14292520247737017No Hit
GATGTGCAGGTAGCTCGGG30.14292520247737017No Hit
TAAGGCGCGGCTTGGTAACCCTAAGGTACGG30.14292520247737017No Hit
AAGCTGCCCGTCCCGTACTTTGTAACG30.14292520247737017No Hit
TGCAGGTAGCTCAGGTGGTAAGGCGCG30.14292520247737017No Hit
GCAGGTAGCTCGGGTGGTAAGGCGCGGC30.14292520247737017No Hit
GATGTGCAGGTAGCTCGGGTGGTAAGGC30.14292520247737017No Hit
GTAGCTCGGGTGGTAAGGCGCGGCT30.14292520247737017No Hit
AGCACAGTATTTTGGCCAGCAACTC30.14292520247737017No Hit
CCGCACCGGGCTACT30.14292520247737017No Hit
ACGAGCTGCCCGTCCCGTACTTTGTAAC30.14292520247737017No Hit
TAACCCTAAGGTCACGGGTTCGAACCCCGC30.14292520247737017No Hit
CACCGGGCTACTTTTT30.14292520247737017No Hit
AGGCGCGGCTTGGTAACCCTAAG30.14292520247737017No Hit
TCGGGTGGTAAGGCGCGGCTTGGTAACCCT30.14292520247737017No Hit
CCGCACCGGGCTACTTTT30.14292520247737017No Hit
AGGCGCGGCTTGGTAAC30.14292520247737017No Hit
TCGGGTGGTAATGCGCGGCTTGGTAACCC30.14292520247737017No Hit
TTCATGAGCTCTAGGATACCTACTCCCAGT30.14292520247737017No Hit
GGTAAGGCGCGGCTTGGTAAC30.14292520247737017No Hit
AGTACGGGACGGGCAGCTTGT30.14292520247737017No Hit
CAGGTAGCTCAGGTGGTAAGGCGCGGC30.14292520247737017No Hit
GTAAGGCGCGGCTTGGTAAC30.14292520247737017No Hit
TCCGGACATTGTTTGAGGCGAACGGTAG30.14292520247737017No Hit
AGGTAGCTCGGGTGGTAAGGCGCGGCTTGGTA30.14292520247737017No Hit
TCCGGACATTGTTTGAGGCGAACGGTAGG30.14292520247737017No Hit
AAGGCGCGGCTTGGTAACCC30.14292520247737017No Hit
AGATGTGTAGGTAGCTCGGGTGGTAAGGCGCGGCT30.14292520247737017No Hit
AAGCTGCCCGTCCCGTACTCTGTAACGGA30.14292520247737017No Hit
CCGCACCGGGCTACTTTTT30.14292520247737017No Hit
CTCGGGTGGTAAGGCGCGGCTTGGTAAC30.14292520247737017No Hit
TAGTGTGGTAAGGCGCGGCTTGGTAACCC30.14292520247737017No Hit
TGTAGGTAGCTCGGGTGGTAAGGCGCGGCTT30.14292520247737017No Hit
ACAAGCTGCCCGTCCCG30.14292520247737017No Hit
GTCACGGGTTCGAACCCCGCACCGGGC30.14292520247737017No Hit
TACAAAGTACGGGACGGGCAGCTTGTGTT30.14292520247737017No Hit
TGTAGGTAGCTCGGGTGGTAAGGC30.14292520247737017No Hit
TGCAGGTAGCTCTGGTGGTAAGGCGCGGC30.14292520247737017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)