FastQCFastQC Report
Mon 16 Nov 2020
S34.15-35nt.16656.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.16656.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185
Sequences flagged as poor quality0
Sequence length16-34
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTCAGACTAGAGACAACCCGAGAGC63328.97025171624714No Hit
TTCCTGTCAGACTAGAGACAACCCGAGAGC1979.016018306636155No Hit
TTTTCCTGTCAGACTAGAGACAACCCGAG924.2105263157894735No Hit
TCCTGTCAGACTAGAGACAACCCGAGAG532.4256292906178487No Hit
TCAGTTAGTACACCACACCGGGAGACGAC522.379862700228833No Hit
TAGGTTCTTGAGAATATCGGCACGGAGC482.196796338672769No Hit
TCAGACTAGAGACAACCCGAGAGCTCAAT462.1052631578947367No Hit
TTTTCCTGTCAGACTAGAGACAACCCGAGA431.9679633867276887No Hit
TTGGAGGAAAACTACGGCTCAACCCGGGC371.6933638443935928No Hit
TTTCCTGTCAGACTAGAGACAACCCGAGA341.5560640732265447No Hit
TTCCTGTCAGACTAGAGACAACCCGAGAG301.3729977116704806No Hit
TTTCCTGTCAGACTAGAGACAACCCGAGAG281.2814645308924484No Hit
TCAGACTAGAGACAACCCGAGAGCTCAATA251.1441647597254003No Hit
TCAGACTAGAGACAACCCGAGAGCTCAATAT221.0068649885583525No Hit
TTTCCTGTCAGACTAGAGACAACCCGAGAGC190.8695652173913043No Hit
TTCTTGAGAATATCGGCACGGAGCTCGC160.7322654462242564No Hit
TTTGGAGGAAAACTACGGCTCAACCCGGGC150.6864988558352403No Hit
TTCGTAATCCGCCTCACCTGTCCCTAACT150.6864988558352403No Hit
TAGGTTCTTGAGAAAATCGGCACGGAGC140.6407322654462242No Hit
TTTTCCTGTCAGACTAGAGACAACCCGAGAG130.5949656750572082No Hit
TGCCCATGTTGCCTCCCGATTAGTACTCGAA130.5949656750572082No Hit
CCTGTCAGACTAGAGACAACCCGAGAGC120.5491990846681922No Hit
TCAGACTAGAGACAACCCGAGAGCTCAA110.5034324942791762No Hit
TCCCCTGGCTGTACCAATTGCCTGACAAAT110.5034324942791762No Hit
CTTTTCCTGTCAGACTAGAGACAACCCGAG100.4576659038901602No Hit
TCCTGTCAGACTAGAGACAACCCGAGA100.4576659038901602No Hit
TTCCTGTCAGACTAGAGACAACCCGAGA90.41189931350114417No Hit
TTCTTGAGAATATCGGCACGGAGCTCGCA90.41189931350114417No Hit
TTAACGGTTTGGTCACCACACTTGAATGCC80.3661327231121282No Hit
TTGGAGGAAAACTACGGCTCAACCCGTGCT80.3661327231121282No Hit
TCTTGAGAAAATCGGCACGGAGCTCGCATC80.3661327231121282No Hit
TCCAGAGTTTGCCCATGTTGCCTCCCAATT70.3203661327231121No Hit
TCTTGAGAATATCGGCACGGAGCTCGCATC70.3203661327231121No Hit
TTGGTCAAGCAGGGCAGGATTAGATAGG70.3203661327231121No Hit
TGCATACGCCCTTCTCTATACTGCCGAAAC70.3203661327231121No Hit
TCATAGAACCTGAACCACGGTAAACCAATG70.3203661327231121No Hit
TTTCCTGTCAGACTAGAGACAACCCGAG70.3203661327231121No Hit
TTGGAGGAAAACTACGGCTCAACCCGGGCT70.3203661327231121No Hit
TCCAAGGACCCATCTCTCACGTGATTAGCA60.2745995423340961No Hit
TCGTAATCCGCCTCACCTGTCCCTAAC60.2745995423340961No Hit
TTGACGCCTTCATTCCCTCTGGCAGCGCACC60.2745995423340961No Hit
TCACAAGTGTCCTGCATTCCTCTCTTGAGA60.2745995423340961No Hit
TTCTTGAGAAAATCGGCACGGAGCTCGC60.2745995423340961No Hit
TGACGCCTTCATTCCCTCTGGCAGCGCACC60.2745995423340961No Hit
TAGGTTCTTGAGAAAATCGGCACGGAGCT60.2745995423340961No Hit
TCGTAATCCGCCTCACCTGTCCCTAACTG60.2745995423340961No Hit
TAGTACACCACACCGGGAGACGACATGTC60.2745995423340961No Hit
TTGGAGGAAAACTACGGCTCAACCCGTGC50.2288329519450801No Hit
TTGCCCATGTTGCCTCCCGATTAGTACTC50.2288329519450801No Hit
TCGATACAATCTCTCAACCTGTTGCTTT50.2288329519450801No Hit
TATTTGGTCAAGCAGGGCAGGATTAGAT50.2288329519450801No Hit
TCCAAGGACCCATCTCTCACGTGATTAGC50.2288329519450801No Hit
TCAGATTCGTAATCCGCCTCACCTGTCCC50.2288329519450801No Hit
TTTGGAGGAAAACTACGGCTCAACCCGTGC50.2288329519450801No Hit
TATGGGTATTTGGTCAAGCAGGGCAGGAT50.2288329519450801No Hit
TATGACAGACCAGAAACATTGCCCAAACTA40.1830663615560641No Hit
TGCCACCACTGGTGCATACGCCCTTCTC40.1830663615560641No Hit
TGTCAGACTAGAGACAACCCGAGAGCTCAAT40.1830663615560641No Hit
TACGCCCTTCTCTATACTGCCGAAACTGGT40.1830663615560641No Hit
GTTCTTGAGAATATCGGCACGGAGCTCGC40.1830663615560641No Hit
TCCAGAGTTTGCCCATGTTGCCTCCCGATT40.1830663615560641No Hit
TCCTGTCAGACTAGAGACAACCCGAGAGCTCAA40.1830663615560641No Hit
TGTAATCATAGAACCTGAACCACGGTAAACC40.1830663615560641No Hit
TTTTCCTGTCAGACTAGAGACAACCCGAGAGC40.1830663615560641No Hit
TGTTTGACGCCTTCATTCCCTCTGGCAGC40.1830663615560641No Hit
TCATTCCCTCTGGCAGCGCACCTTTTCCT40.1830663615560641No Hit
TGTTTGACGCCTTCATTCCCTCTGGCA40.1830663615560641No Hit
TTTGCCCATGTTGCCTCCCGATTAGTACTCG40.1830663615560641No Hit
TTGGTCAAGCAGGGCAGGATTAGATAGGA40.1830663615560641No Hit
TGCATACGCCCTTCTCTATACTGCCGAAACT40.1830663615560641No Hit
TCCTGTCAGACTAGAGACAACCCGAGAGCTCA30.13729977116704806No Hit
CACCTTTTCCTGTCAGACTAGAGACAACC30.13729977116704806No Hit
AACGGTTTGGTCACCACACTTGAATGCCC30.13729977116704806No Hit
TCCGCCTCACCTGTCCCTAACTG30.13729977116704806No Hit
TTCCTGTCAGACTAGAGACAACCCGAG30.13729977116704806No Hit
TTGGTCGTCACCTCCGAGATCTAGTAGG30.13729977116704806No Hit
CGTAATCCGCCTCACCTGTCCCTAACTGG30.13729977116704806No Hit
TAAGAAACCTGGTGTGTGGGTGGT30.13729977116704806No Hit
TTGAGAAAATCGGCACGGAGCTCGCATC30.13729977116704806No Hit
TGCCCATGTTGCCTCCCGATTAGTACTC30.13729977116704806No Hit
TTGAGAAAATCGGCACGGAGCTCGCATCG30.13729977116704806No Hit
TCTTGAGAATATCGGCACGGAGCTCGCA30.13729977116704806No Hit
GCATTCCTCTCTTGAGA30.13729977116704806No Hit
TAGGTTCTTGAGAATATCGGCACGGAGCT30.13729977116704806No Hit
TGACAGAATGTTTGACGCCTTCATTCCCTC30.13729977116704806No Hit
TCATTGAAACATCTGTCACCTAGTGGTA30.13729977116704806No Hit
TACGCAAAATCTTCTGGGCTAACTTTGGCC30.13729977116704806No Hit
GACACCCTCGTGTTCTCTTAACTCCTTGAAA30.13729977116704806No Hit
CACCTTCACAAGCTTGTCTCCTTCTGGG30.13729977116704806No Hit
TTCGTAATCCGCCTCACCTGTCCCTAACTG30.13729977116704806No Hit
TCCAGAAGAGAATGAACCCCAAGCTTGAA30.13729977116704806No Hit
TGTGTACCACCTTCACAAGCTTGTCTCCTT30.13729977116704806No Hit
TATGACAGACCAGAAACATTGCCCTAACTA30.13729977116704806No Hit
TTCTTGAGAATATCGGCACGGAGCTC30.13729977116704806No Hit
TGACGCCTTCATTCCCTCTGGCAGCGCA30.13729977116704806No Hit
TATCAGTTAGTACACCACACCGGGAGAC30.13729977116704806No Hit
TAAGAAACCTGGTGTGTGGGTGGTAGATT30.13729977116704806No Hit
GCAGACTATCAGTTAGTACACCACACCGGG30.13729977116704806No Hit
TTTGCCCATGTTGCCTCCCGATTAGTACTC30.13729977116704806No Hit
GCCGGGGTGCTCAGTCTTTAGGGATT30.13729977116704806No Hit
CCTGTCAGACTAGAGACAACCCGAGAG30.13729977116704806No Hit
TGACGCCTTCATTCCCTCTGGCAGCGCACCC30.13729977116704806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)