FastQCFastQC Report
Mon 30 Nov 2020
S34.15-35nt.2812.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.2812.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61
Sequences flagged as poor quality0
Sequence length15-34
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTGAAGAAACTGTGCCCGGAGA914.754098360655737No Hit
CCCCTGTGAAGAAACTGTGCCCGGAGA69.836065573770492No Hit
GTCCGACGGCCCCTG58.19672131147541No Hit
CCTGTGAAGAAACTGTGCCCGGAGAA46.557377049180328No Hit
TAGGCAGGCAAGTAGAATGGTATAAAGCT34.918032786885246No Hit
TCATCGCAGAACACCACGACCACGTCCACG34.918032786885246No Hit
TTCATCGAGCAGCAGCGTGGTCGCGTCAGC34.918032786885246No Hit
CATCGCAGAACACCACGACCACGTCCACG23.278688524590164No Hit
TCTTAGGCAGGCAAGTAGAATGGTATAAAG23.278688524590164No Hit
CCTGTGAAGAAACTGTGCCCGGAG23.278688524590164No Hit
CCCCTGTGAAGAAACTGTGCCCGGAGAA23.278688524590164No Hit
TCCGGAGCCAAAGTGCAAGTGCATTTGACT11.639344262295082No Hit
CCCCTGTGAAGAAACTGTGCCCGGAGAAAGC11.639344262295082No Hit
CCTGTGAAGAAACTGTGCCCGGAGAAAGC11.639344262295082No Hit
TATCGCGTAGTCCTCTTGAAAGTATTTCT11.639344262295082No Hit
CCCCTGTGAAGAAACTGTGCCCGGAG11.639344262295082No Hit
AGACCGACGGCCCCTGT11.639344262295082No Hit
CCTGTGAAGAAACTGTGCCCGGA11.639344262295082No Hit
GAGATTACTACGCAACAATTGACCCTG11.639344262295082No Hit
AAGAAACTGTGCCCGGAGA11.639344262295082No Hit
TATTCCCCGGCATGAATTCACAATTAAGT11.639344262295082No Hit
CCTGTGAAGAAACTGTGCCCGGAGAAT11.639344262295082No Hit
AGGAAGTCTACTGGAAGCAAGCAACT11.639344262295082No Hit
CCCTGTGAAGAAACTGTGCCCGGAGA11.639344262295082No Hit
CTGTGAAGAAACTGTGCCCGGAGAAAGC11.639344262295082No Hit
GACTATAGGTCTGCTGCCCCCACTATT11.639344262295082No Hit
CCTGTGAAGAAACTGTGCCCGGAGAACC11.639344262295082No Hit
AACACCACGACCACGTCCACGTTATACACGACTG11.639344262295082No Hit
TCCCCTGTGAAGAAACTGTGCCCGGAG11.639344262295082No Hit
GACAGTGCAGATGTG11.639344262295082No Hit
CCGACGGCCCCTGTGAAGAAACTGTGCC11.639344262295082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)