FastQCFastQC Report
Mon 30 Nov 2020
S34.15-35nt.2812.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.2812.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872
Sequences flagged as poor quality0
Sequence length15-35
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCCGTCTGCGCACTGGATGAAACCGT1756.093314763231198No Hit
TTCTTGAAGTCCCGAGTTATCGCTTTCTG1434.979108635097493No Hit
TCCCGAGTTATCGCTTTCTGTAATTGTCTC792.7506963788300838No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACATC652.2632311977715878No Hit
TACTTAATTGTGAACTCATGCCGGGGAA642.2284122562674096No Hit
TAACTCTTCGTCCGACTCGACATCCATA592.054317548746518No Hit
TGCTTCCAGTAGACTTCCTTCGGTTGACCT551.915041782729805No Hit
TCTTGAAGTCCCGAGTTATCGCTTTCTG541.8802228412256268No Hit
TCCATAATTGGCGGTTTGCTGGTAGATA511.7757660167130918No Hit
TTCAACGGCGCAGCAGGGTGGTTCCGAAC491.7061281337047354No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACA461.6016713091922006No Hit
TCGTGTATAACGTGGACGTGGTCGTGGT361.2534818941504178No Hit
TAGATATGGGCACATCTGCACTGGTTGGAT351.2186629526462396No Hit
TCGTGTATAACGTGGACGTGGTCGTGGTG351.2186629526462396No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGAGT331.149025069637883No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGAG311.0793871866295264No Hit
TCCTCCCATACTTGCGCTTGCTGATCGGG270.9401114206128134No Hit
TCCGGGCACAGTTTCTTCACAGGGGACGTC250.8704735376044569No Hit
TCATCTCCAGATTCGCATGCTGACGCGAC240.8356545961002786No Hit
TGCCCCGTCTGCGCACTGGATGAAACC240.8356545961002786No Hit
TACTTAATTGTGAACTCATGCCGGGGAAT230.8008356545961003No Hit
TGCCCCGTCTGCGCACTGGATGAAACCG230.8008356545961003No Hit
TGCTTGCTTCCAGTAGACTTCCTTCGGT220.766016713091922No Hit
TAATTGGCGGTTTGCTGGTAGATATGGGC220.766016713091922No Hit
TTTGCTGGTAGATATGGGCACATCTGCACTG220.766016713091922No Hit
TGCTGATCGGGAGTCAAATGCACTTGCACT200.6963788300835655No Hit
TCTCCATAATCCATTAGTGACCAAGCCGCC180.6267409470752089No Hit
TTCTGTAATTGTCTCAACAGTTGCCCCGTC180.6267409470752089No Hit
TATGGGCACATCTGCACTGGTTGGATT170.5919220055710306No Hit
TGCTGGTAGATATGGGCACATCTGCACT160.5571030640668524No Hit
TTGCCCCGTCTGCGCACTGGATGAAACCG160.5571030640668524No Hit
TTCACAGGGGCCGTCGGTCTGGGACACTC160.5571030640668524No Hit
TGACCAAGCCGCCAATTCAATTGCTCTGTT160.5571030640668524No Hit
TTGCCCCGTCTGCGCACTGGATGAAACCGT140.48746518105849584No Hit
TGAAGTCCCGAGTTATCGCTTTCTGTAATT140.48746518105849584No Hit
TATGGGCACATCTGCACTGGTTGGATTG130.45264623955431754No Hit
TGAAATATGAGTTTCAACGGCGCAGCAGGG130.45264623955431754No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACAT130.45264623955431754No Hit
TGTCTCAACAGTTGCCCCGTCTGCGCACT130.45264623955431754No Hit
TCTGTAATTGTCTCAACAGTTGCCCCGTC130.45264623955431754No Hit
TAGTAATTGTTCTCCCGTATTTCTGGGGC120.4178272980501393No Hit
TTGGCGGTTTGCTGGTAGATATGGGCAC120.4178272980501393No Hit
TGCTTGCTTCCAGTAGACTTCCTTCGGTT110.383008356545961No Hit
TCACAGGGGCCGTCGGTCTGGGACACTC110.383008356545961No Hit
TGCTTCCAGTAGACTTCCTTCGGTTGACC110.383008356545961No Hit
TCGTCCGACTCGACATCCATAATTGGCGGT110.383008356545961No Hit
TTCGAGCTTCATTACCAAATCGTGGGAT100.34818941504178275No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGA100.34818941504178275No Hit
TCCGGGCACAGTTTCTTCACAGGGGCCGTC100.34818941504178275No Hit
TCGACATCCATAATTGGCGGTTTGCTGGT100.34818941504178275No Hit
TTCGTCCGACTCGACATCCATAATTGGCGGT90.31337047353760444No Hit
TGCTGGTAGATATGGGCACATCTGCACTG90.31337047353760444No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTGA90.31337047353760444No Hit
TATGAGCTTCAACGGCGCAGCAGGGTGG90.31337047353760444No Hit
TTGTCTCAACAGTTGCCCCGTCTGCGCAC90.31337047353760444No Hit
TCATCTCCAGATTCGCATGCTGACGCGACC90.31337047353760444No Hit
TTCGAGCTTCATTACCAAATCGTGGGATC90.31337047353760444No Hit
TATGGGCACATCTGCACTGGTTGGATTGAT90.31337047353760444No Hit
TCTACTTGCCTGCCTATTTTAGCCCCACA90.31337047353760444No Hit
TTTGCTGGTAGATATGGGCACATCTGCACT80.2785515320334262No Hit
TCACAGGGGACGTCGGTCTGGGACACTC80.2785515320334262No Hit
TTGTAGATCGTCTGGAACTTGGTAAGAGA80.2785515320334262No Hit
TCTTCACAGGGGACGTCGGTCTGGGACACT80.2785515320334262No Hit
TCGGAAGAAGAGCATCCCATACTTGTTGAGT80.2785515320334262No Hit
TAAGTCTTCGTCCGACTCGACATCCATA80.2785515320334262No Hit
TTCCTCCCATACTTGCGCTTGCTGATCGG80.2785515320334262No Hit
TGCTGGTAGATATGGGCACATCTGCACTGG80.2785515320334262No Hit
TCTTCACAGGGGACGTCGGTCTGGGACACTC80.2785515320334262No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTG80.2785515320334262No Hit
TCCGAACGAACTCGAGTAATTGTTCTCCCG70.24373259052924792No Hit
TGCGCTTGCTGATCGGGAGTCAAATGCACT70.24373259052924792No Hit
TACTTAATTGTGAACTCATGCCGGGGA70.24373259052924792No Hit
TCTCAACAGTTGCCCCGTCTGCGCACTGG70.24373259052924792No Hit
TCGACATCCATAATTGGCGGTTTGCTGGTA70.24373259052924792No Hit
TAGATATGGGCACATCTGCACTGGTTGGA70.24373259052924792No Hit
TCTTCACAGGGGCCGTCGGTCTGGGACACTC70.24373259052924792No Hit
TCCGGGCACAGTTTCTTCACAGGGGACG70.24373259052924792No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTT70.24373259052924792No Hit
ACTGCCCATCTCGCCTTTCTTGAAGTCCCG60.20891364902506965No Hit
TATCGCTTTCTGTAATTGTCTCAACAGTT60.20891364902506965No Hit
TCCTTCGGTTGACCTACACATTTCGAGTA60.20891364902506965No Hit
TCTTGAAGTCCCGAGTTATCGCTTTCTGT60.20891364902506965No Hit
TTGTCTCAACAGTTGCCCCGTCTGCGCACT60.20891364902506965No Hit
TCGGTCTGGGACACTCTTGGAAGGTCAGC60.20891364902506965No Hit
TACTTAATTGTGAATTCATGCCGGGGAA60.20891364902506965No Hit
TCCAAGTTGCTTGCTTCCAGTAGACTTCCT60.20891364902506965No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACT60.20891364902506965No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCG60.20891364902506965No Hit
TAGTGACCAAGCCGCCAATTCAAT60.20891364902506965No Hit
TCCGGGCACAGTTTCTTCACAGGGGCCGT60.20891364902506965No Hit
TGTTGTAGATCGTCTGGAACTTGGTAAGA60.20891364902506965No Hit
TTCTTCACAGGGGCCGTCGGTCTGGGACA60.20891364902506965No Hit
TGTTCCTCCCATACTTGCGCTTGCTGATCGG50.17409470752089137No Hit
TCTGCACTGGTTGGATTGATAGGAACAGTC50.17409470752089137No Hit
TCTCCATAATCCATTAGTGACCAAGCCGCCT50.17409470752089137No Hit
TCTTCACAGGGGCCGTCGGTCTGGGACACT50.17409470752089137No Hit
TATATTGTTGTAGATCGTCTGGAACTTGGT50.17409470752089137No Hit
TTCATCTCCAGATTCGCATGCTGACGCGAC50.17409470752089137No Hit
TATTGTTGTAGATCGTCTGGAACTTGGT50.17409470752089137No Hit
TTCGAGCTTCATTACCAAATCGTGGGATCGA50.17409470752089137No Hit
TAATTGTCTCAACAGTTGCCCCGTCTGC50.17409470752089137No Hit
TATAACGTGGACGTGGTCGTGGTGTTCTG50.17409470752089137No Hit
TGAAAAGCCATGAAGGACCAATTGGTATAGT50.17409470752089137No Hit
TGTCTCAACAGTTGCCCCGTCTGCGCACTG50.17409470752089137No Hit
TCCCGAGTTATCGCTTTCTGTAATTGTCTCAAC50.17409470752089137No Hit
TCACAGGGGCCGTCGGTCTGGGACACTCT50.17409470752089137No Hit
TGAAATATGAGCTTCAACGGCGCAGCAGGG50.17409470752089137No Hit
TCCTTCGGTTGACCTACACATTTCGAGTAA50.17409470752089137No Hit
TCCTTCGGTTGACCTACACATTTCGAGT50.17409470752089137No Hit
CTTCCTTCGGTTGACCTACACATTTCGAGTA50.17409470752089137No Hit
TGGGCACATCTGCACTGGTTGGATTGATAG50.17409470752089137No Hit
TACAGTTTGAAGAATTCTAGCACCACAAT50.17409470752089137No Hit
TGTTGTAGATCGTCTGGAACTTGGTAAGAG50.17409470752089137No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTC50.17409470752089137No Hit
TACTTGCGCTTGCTGATCGGGAGTCAAAT40.1392757660167131No Hit
TATAACGTGGACGTGGTCGTGGTGTTCTGC40.1392757660167131No Hit
TCTTCGTCCGACTCGACATCCATAATTGGC40.1392757660167131No Hit
TCCGAACGAACTCGAGTAATTGTTCTCCC40.1392757660167131No Hit
TAGTGGGGGCAGCAGACCTATAGTCACGC40.1392757660167131No Hit
TAGATATGGGCACATCTGCACTGGTTGG40.1392757660167131No Hit
TGCTCGATGAAATAGTGGGGGCAGCAGACCT40.1392757660167131No Hit
TTCCTCTTCGCCTTCTTCATTAGAGG40.1392757660167131No Hit
ACTGCCCATCTCGCCTTTCTTGAAGTCCCGA40.1392757660167131No Hit
TTGCTGGTAGATATGGGCACATCTGCACT40.1392757660167131No Hit
TTCGGCCCATAGCATTGACATTTTCTTCATC40.1392757660167131No Hit
TTTGCTGGTAGATATGGGCACATCTGCAC40.1392757660167131No Hit
TTCTCCGGGCACAGTTTCTTCACAGGGGCC40.1392757660167131No Hit
TAATTGGCGGTTTGCTGGTAGATATGGGCACA40.1392757660167131No Hit
TTAAACTGCCCATCTCGCCTTTCTTGAAG40.1392757660167131No Hit
TTCACAGGGGACGTCGGTCTGGGACACTC40.1392757660167131No Hit
TCCGAACGAACTCGAGTAATTGTTCTCCCGT40.1392757660167131No Hit
TAAACTGCCCATCTCGCCTTTCTTGAAGT40.1392757660167131No Hit
TCGCTTTCTGTAATTGTCTCAACAGTT40.1392757660167131No Hit
TCGTGTATAACGTGGACGTGGTCGTGG40.1392757660167131No Hit
TGGTTGGATTGATAGGAACAGTCGTGTAT40.1392757660167131No Hit
TCGTGTATAACGTGGATGTGGTCGTGGT40.1392757660167131No Hit
ATACTTAATTGTGAACTCATGCCGGGGAA40.1392757660167131No Hit
CTCGACATCCATAATTGGCGGTTTGCTGGT40.1392757660167131No Hit
CCCCGTCTGCGCACTGGATGAAACCGT40.1392757660167131No Hit
TTGTGAACTCATGCCGGGGAATAACTCTTC40.1392757660167131No Hit
TTCTGAAATTGTCTCAACAGTTGCCCCGTC40.1392757660167131No Hit
TGAGTACAGTTTGAAGAATTCTAGCACCAC40.1392757660167131No Hit
TCCATAATCCATTAGTGACCAAGCCGCCAAT40.1392757660167131No Hit
TATTGACCCAACTAGACTAGTAGAACTATT40.1392757660167131No Hit
TGTAGATCGTCTGGAACTTGGTAAGAGAT40.1392757660167131No Hit
TCGCATGCTGACGCGACCACGCTGCTGC40.1392757660167131No Hit
AGTTTGAAGAATTCTAGCACCACGATGAA40.1392757660167131No Hit
TACTTAATTGTGAATTCATGCCGGGGAAT30.10445682451253482No Hit
TTGGATTGATAGGAACAGTCGTGTATAACGT30.10445682451253482No Hit
CTCGACATCCATAATTGGCGGTTTGCTG30.10445682451253482No Hit
CATACTTAATTGTGAATTCATGCCGGGG30.10445682451253482No Hit
TAGGAACAGTCGTGTATAACGTGGACGTG30.10445682451253482No Hit
TCCGGGCACAGTTTCTTCACAGGGGACGT30.10445682451253482No Hit
TTGACGGCATACTTAATTGTGAACTCATGC30.10445682451253482No Hit
TCAACGGCGCAGCAGGGTGGTTCCGAACGA30.10445682451253482No Hit
TAGTGGGGGCAGCAGACCTATAGTCACGCC30.10445682451253482No Hit
TTCACAGGGGCCGTCGGTCTGGGACACTCTT30.10445682451253482No Hit
TCGGAAGAAGAGCATCCCATACTTGTTGAG30.10445682451253482No Hit
TATGTATTTGATGCGCTATTTCGGCCCAT30.10445682451253482No Hit
TGCGTAGTAATCTCCATAATCCATTAGTG30.10445682451253482No Hit
TCCATAATCCATTAGTGACCAAGCCGCCAA30.10445682451253482No Hit
TGTTGAGTACAGTTTGAAGAATTCTAGCAC30.10445682451253482No Hit
TTCCAGTAGACTTCCTTCGGTTGACCTA30.10445682451253482No Hit
TTGCTTCCAGTAGACTTCCTTCGGTTGACT30.10445682451253482No Hit
TGGTAGATATGGGCACATCTGCACTGGTT30.10445682451253482No Hit
TCTTGGTCCTTTTTCCAAATAGAGCACGG30.10445682451253482No Hit
TGAAACCGTGCACAATGTCTTCCAAGTTGC30.10445682451253482No Hit
TCTGCACTGGTTGGATTGATAGGAACAGT30.10445682451253482No Hit
TGCCCCGTCTGCGCACTGGA30.10445682451253482No Hit
TGCGCTTGCTGATCGGGAGTCAAATGC30.10445682451253482No Hit
TTCTTCACAGGGGCCGTCGGTCTGGGAC30.10445682451253482No Hit
TCCGAACGAACTCTAGTAATTGTTCTCCCG30.10445682451253482No Hit
TTTGCTGGTAGATATGGGCACATCTGCACTGC30.10445682451253482No Hit
TCTGGGACACTCTTGGAAGGTCAGCTTCCT30.10445682451253482No Hit
TCACAGGGGCCGTCGGTCTGGGACACTCTT30.10445682451253482No Hit
TAATTGTGAACTCATGCCGGGGAATAACT30.10445682451253482No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTGCATT30.10445682451253482No Hit
TTAGTGACCAAGCCGCCAATTCAATTGCTCT30.10445682451253482No Hit
TCCATAATTGGCGGTTTGCTGGTAGATATG30.10445682451253482No Hit
TTCAACGGCGCAGCAGGGTGGTTCCGAACT30.10445682451253482No Hit
TGGTCCAGTGAGGCGTTTGAAATATGAG30.10445682451253482No Hit
TTAATTGTGAACTCATGCCGGGGAATAAC30.10445682451253482No Hit
TAATGATATTGACCCAACTAGACTAGTAGA30.10445682451253482No Hit
CTCGACATCCATAATTGGCGGTTTGCTGGTA30.10445682451253482No Hit
TGACATTTTCTTCATCTCCAGATTCGCAT30.10445682451253482No Hit
TTGGTATAGTCGGCTTCTTTCATAATTAT30.10445682451253482No Hit
TGTCTCAACAGTTGCCCCGTCTGCGCAC30.10445682451253482No Hit
TGTAGATCGTCTGGAACTTGGTAAGAG30.10445682451253482No Hit
TGCTGATCGGGAGTCAAATGCACTTGCACTT30.10445682451253482No Hit
TCCAGATTCGCATGCTGACGCGACCACGC30.10445682451253482No Hit
TCCATTAGTGACCAAGCCGCCAATTCAAT30.10445682451253482No Hit
AGTTTCTTCACAGGGGACGTCGGTCTGG30.10445682451253482No Hit
TTCATCTCCAGATTCGCATGCTGACGCGACC30.10445682451253482No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTTT30.10445682451253482No Hit
TTCTTCACAGGGGCCGTCGGTCTGGGACAC30.10445682451253482No Hit
TTCTTGAAGTCCCGAGTTATCGCTTTCT30.10445682451253482No Hit
TTCTTCACAGGGGCCGTCGGTCTGGG30.10445682451253482No Hit
TTGGTCCAGTGAGGCGTTTGAAATATGAGC30.10445682451253482No Hit
TGGTGTTCTGCGATGACGGTAAATGCGT30.10445682451253482No Hit
TGTATAACGTGGACGTGGTCGTGGTGTT30.10445682451253482No Hit
TTAATTGTGAACTCATGCCGGGGAATAACT30.10445682451253482No Hit
TGAGTACAGTTTGAAGAATTCTAGCACCA30.10445682451253482No Hit
TAATTGGCGGTTTGCTGGTAGATATGGG30.10445682451253482No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTGAA30.10445682451253482No Hit
TCAAATGCACTTGCACTTTGGCTCCGGAT30.10445682451253482No Hit
CCGGGGAATAACTCTTCGTCCGACTCGACA30.10445682451253482No Hit
TACTTAATTGTGAATTCATGCCGGGGA30.10445682451253482No Hit
TAAACTGCCCATCTCGCCTTTCTTGAAGTC30.10445682451253482No Hit
TGTAAAACGTTTGCTTTGACAGGTATCCT30.10445682451253482No Hit
ACAGTTTGAAGAATTCTAGCACCACGATG30.10445682451253482No Hit
TTAAACTGCCCATCTCGCCTTTCTTGAAGT30.10445682451253482No Hit
TTCGTCCGACTCGACATCCATAATTGGCGG30.10445682451253482No Hit
TTCGCATGCTGACGCGACCACGCTGCTGC30.10445682451253482No Hit
TTGTTGTAGATCGTCTGGAACTTGGTAAG30.10445682451253482No Hit
TTAGTGACCAAGCCGCCAATTCAATTGCTC30.10445682451253482No Hit
TTTTCTTCATCTCCAGATTCGCATGCTGAC30.10445682451253482No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTGCACC30.10445682451253482No Hit
TCGTGTATAACGTGGACGTGGTCG30.10445682451253482No Hit
TCTCCATAATCCATTAGTGACCAAGCCGCCAAT30.10445682451253482No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)