FastQCFastQC Report
Mon 30 Nov 2020
S34.15-35nt.2829.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.2829.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594
Sequences flagged as poor quality0
Sequence length15-35
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCCACTCTCGTTGCTCCTCTGGCATA528.754208754208754No Hit
TACCCACTCTCGTTGCTCCTCTGGCATAGCC366.0606060606060606No Hit
TACCCACTCTCGTTGCTCCTCTGGCATAGC294.882154882154882No Hit
TGTTCTACCCACTCTCGTTGCTCCTCTGGC274.545454545454546No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCAAG244.040404040404041No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCA233.872053872053872No Hit
ATAGGACGGAGCACCTGACCCTGCCAGG172.861952861952862No Hit
TCTCGTTGCTCCTCTGGCATAGCCCCACAG142.356902356902357No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGCA132.1885521885521886No Hit
TGTGTTGTTCTACCCACTCTCGTTGCTCC132.1885521885521886No Hit
TTCTACCCACTCTCGTTGCTCCTCTGGC122.0202020202020203No Hit
TACCCACTCTCGTTGCTCCTCTGGCATAG111.8518518518518516No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCAA101.6835016835016834No Hit
TCTACCCACTCTCGTTGCTCCTCTGGCATAG101.6835016835016834No Hit
GAAAGAAACAGTTAAGGGAGGACCACTC91.5151515151515151No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCAC71.1784511784511784No Hit
CCACTCTCGTTGCTCCTCTGGCATAGCCCC71.1784511784511784No Hit
TTCTACCCACTCTCGTTGCTCCTCTGGCATA61.0101010101010102No Hit
TACCCACTCTCGTTGCTCCTCTGGCATAGCCC61.0101010101010102No Hit
TCCATGCTATCCTGGGTTCCCCACACTGGC61.0101010101010102No Hit
TGTTGTTCTACCCACTCTCGTTGCTCCTC50.8417508417508417No Hit
TAGGACGGAGCACCTGACCCTGCCAGG50.8417508417508417No Hit
TGGTCAATCGCTAAAAGAACGCTGTGTTGT50.8417508417508417No Hit
TCTCGTTGCTCCTCTGGCATAGCCCCACA50.8417508417508417No Hit
TGTGTTGTTCTACCCACTCTCGTTGCT50.8417508417508417No Hit
TTCTACCCACTCTCGTTGCTCCTCTG50.8417508417508417No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCATA40.6734006734006733No Hit
TGATTGTGGTCAATCCCATGCACCCTCCCC40.6734006734006733No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGC40.6734006734006733No Hit
CGCTGTGTTGTTCTACCTACTCTCGTTGCTCCTCT40.6734006734006733No Hit
CATGCTATCCTGGGTTCCCCA30.5050505050505051No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCAT30.5050505050505051No Hit
ATTGCAACGATAGGTAGGAAATCCTCATGGAT30.5050505050505051No Hit
TAACGCGTACTCCGTTAGTAGAGCTCC30.5050505050505051No Hit
TGTGTTGTTCTACCCACTCTCGTTGCTC30.5050505050505051No Hit
TACTCCGTTAGTAGAGCTCCATGCTATCCT30.5050505050505051No Hit
TCTACCCACTCTCGTTGCTCCTCTGGCATAGCC30.5050505050505051No Hit
ACCCACTCTCGTTGCTCCTCTGGCATAGCCC30.5050505050505051No Hit
TGTTGTTCTACCCACTCTCGTTGCTCCT30.5050505050505051No Hit
TGTTCTACCCACTCTCGTTGCTCCT30.5050505050505051No Hit
TAACAATGGTCAATCGCTAAAAGAACTCCGT30.5050505050505051No Hit
ATAGGACGGAGCACCTGACCCTGCC30.5050505050505051No Hit
ACCCACTCTCGTTGCTCCTCTGGCATAGCC30.5050505050505051No Hit
AATAGGACGGAGCACCTGACCCTGCCAGG30.5050505050505051No Hit
TGTGTTGTTTTACCCACTCTCGTTGCTCCT30.5050505050505051No Hit
AACAATGGTCAATCGCTAAAAGAACGCTGT30.5050505050505051No Hit
GTTGTTCTACCCACTCTCGTTGCTCCTCTGGC30.5050505050505051No Hit
TTGTTCTACCCACTCTCGTTGCTCCTCTG30.5050505050505051No Hit
ACGCGTACTCCGTTAGTA30.5050505050505051No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCAAA30.5050505050505051No Hit
TCCTGGGAAAGAAACAGTTAAGGGAGGAC30.5050505050505051No Hit
TGTGTTGTTTTACCCACTCTCGTTGCT20.33670033670033667No Hit
TGTTGTTCTACCCACTCTCGTTGCTCCTCT20.33670033670033667No Hit
TCCATGCTATCCTGGGTTCCCCACACTGG20.33670033670033667No Hit
TACCCACTCTCGTTGCTCCTCT20.33670033670033667No Hit
CATGCACCCTCCCCAAAGTCAGCCAGATT20.33670033670033667No Hit
TGTTCTACCCACTCTCGTTGCTCCTCTGGG20.33670033670033667No Hit
AATAGGACGGAGCACCTGACCCTGCCAGGC20.33670033670033667No Hit
TAGGGCTGGAAAGGATGCGACATACGC20.33670033670033667No Hit
GTACTCCGTTAGTAGAGCTCCA20.33670033670033667No Hit
TAACAATGGTCAATCGCTAAAAGAACGCT20.33670033670033667No Hit
ACTCTCGTTGCTCCTCTGGCATAGCCCCACA20.33670033670033667No Hit
TGTGTTGTTCTATCCACTCTCGTTGCTCC20.33670033670033667No Hit
TCCGTGTTGTTCTACCCACTCTCGTTGCTC20.33670033670033667No Hit
TATAACAATGGTCAATCGCTAAAAGAACGCT20.33670033670033667No Hit
TGCAACGATAGGTAGGAAATCCTCATGGAT20.33670033670033667No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCATC20.33670033670033667No Hit
GTTCTACCCACTCTCGTTGCTCCTCTGGCATA20.33670033670033667No Hit
TGTTCTACCCACTCTCGTTGCTCCTCTGGCA20.33670033670033667No Hit
ACGCGTACTCCGTTAGTAGAGC20.33670033670033667No Hit
TGTTGTTCTACCCACTCTCGT20.33670033670033667No Hit
CTACCCACTCTCGTTGCTCCTCTGGCATAGCC20.33670033670033667No Hit
ACTCTCGTTGCTCCTCTGGCATAGCCCCAC20.33670033670033667No Hit
TGTTCTACCCACTCTCGTTGCTCCTCTGG20.33670033670033667No Hit
CCACTCTCGTTGCTCCTCTGGCATAG20.33670033670033667No Hit
TCAACCCACTCTCGTTGCTCCT20.33670033670033667No Hit
TACTCCAATTTAATTACAATAGTCAGTCAGT20.33670033670033667No Hit
TTCGAAGATCTCCGTGGGCTCCTCTCAGC20.33670033670033667No Hit
TTACCCACTCTCGTTGCTCCTCTGGCATAGC20.33670033670033667No Hit
TAAAAGAACGCTGTGTTGCTCTACCCACTC20.33670033670033667No Hit
TAAAACTGTGGTGAGAATTGCAACGATAGGTAGGA20.33670033670033667No Hit
TACCCACTCTCGTTGCTCCTCTGGC20.33670033670033667No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCAAC20.33670033670033667No Hit
TTGTGGTCAATCCCATGCACCCTCCCCA20.33670033670033667No Hit
CGCGTACTCCGTTAGTAGAGCTCCATGCTA20.33670033670033667No Hit
TGTGTTGTTTTACCCACTCTCGTTGCTCC20.33670033670033667No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCAAGT20.33670033670033667No Hit
TTCTACCCACTCTCGTTGCTCCT20.33670033670033667No Hit
ACAATGGTCAATCGCTAAAAGAACGCTGT20.33670033670033667No Hit
TATCCTGGGTTCCCCGCACTGGCAA20.33670033670033667No Hit
TCCTGGGAAAGAAACAGTTAAGGGAGGACC10.16835016835016833No Hit
CTCGTTGCTCCTCTGGCATAGCCC10.16835016835016833No Hit
TCCTGGGAAAGAAACAGTTAAGGGAGG10.16835016835016833No Hit
TAACAATGGTCAATCGCTAAAAGAACTCC10.16835016835016833No Hit
TCCGTTAGTAGAGCTCCATGCTATCCT10.16835016835016833No Hit
TACTCCGTTAGTAGAGCTCCATGCTATCC10.16835016835016833No Hit
TTCTACCCACTCTCGTTGCTCCTCTGGCATAGCC10.16835016835016833No Hit
CACCCATGGACAAACAGAGCTGGAACAAA10.16835016835016833No Hit
ACGCTGTGTTGTTCTACCCACTCTCGTTGCTCCT10.16835016835016833No Hit
TACCCACTCTCGTTGCTCCTCTGGCATAGCCCCA10.16835016835016833No Hit
TAGGACGGAGCACATGACCCTGCCAGGCAA10.16835016835016833No Hit
TTCACATTGTTGCTTGCTTGGTTTGTTCGC10.16835016835016833No Hit
TCTACCCACTCTCGTTGCTCCTCTGGCATAGC10.16835016835016833No Hit
TCCATGCTATCCTGGGTTCCCCACAC10.16835016835016833No Hit
CCCACTCTCGTTGCTCCTCTGGCATAGCCCC10.16835016835016833No Hit
TTCTACCCACTCTCGTTGCTCCTCTGGCATAG10.16835016835016833No Hit
TAGGACGGAGAACCTGACCCTGCCA10.16835016835016833No Hit
TCTAACGCGTACTCCGTTAGTAGAGCTCC10.16835016835016833No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGCAA10.16835016835016833No Hit
TTCTACCCACTCTCGTTGCTCCTCTGG10.16835016835016833No Hit
TCCATGCTATCCTGGGTTCCCC10.16835016835016833No Hit
AACAATGGTCAATCGCTAAAAGAACTCCGT10.16835016835016833No Hit
TGTGTTGTTCTACCCACTCTCGTTGCTCCA10.16835016835016833No Hit
CTACCCACTCTCGTTGCTCCTCTGGCATAGC10.16835016835016833No Hit
TCTACCCACTCTCGTTGC10.16835016835016833No Hit
TCCATGCTATCCTGGGTTCCCCAC10.16835016835016833No Hit
TAGGACGGAGCACCCGACCCTGCCAGGCAAG10.16835016835016833No Hit
ACCCACTCTCGTTGCTCCTCTGGCATAGCCCC10.16835016835016833No Hit
TGTGTTGTTCTACCCACTCTCGTTGCTCCT10.16835016835016833No Hit
TACCCACTCTCGTTGCTCCTCTG10.16835016835016833No Hit
AGGACGGAGCACCTGACCCTGCCAGGCA10.16835016835016833No Hit
TGCACCCTCCCCAAAGTCAGCCAGATTATGTT10.16835016835016833No Hit
TGTGGTGAGAATTGCAACGATAGGTAGGA10.16835016835016833No Hit
TGCACCCTCCCCAAAGTCAGCCAGATTATGT10.16835016835016833No Hit
TACCCACTCTCGTTGCTCCT10.16835016835016833No Hit
CATGGATAAATGGAAGACCCCAGAGAAAGCCC10.16835016835016833No Hit
TGGTCAATCCCATGCACCCTCCCCAAAGTC10.16835016835016833No Hit
CGCGTACTCCGTTAGTAGAGCTCCATGCTATC10.16835016835016833No Hit
CATCGTCAAGATCCTGCTCCCATTCACCC10.16835016835016833No Hit
CCCACTCTCGTTGCTCCTC10.16835016835016833No Hit
CGCGTACTCCGTTAGTAGAGCTCC10.16835016835016833No Hit
TAGGACGGAGCACCTGACCCTGCCA10.16835016835016833No Hit
CCACTCTCGTTGCTCCTCTGGCATAGCC10.16835016835016833No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGCAAGT10.16835016835016833No Hit
CACTCTCGTTGCTCCTCTGGCATAGCCCCAC10.16835016835016833No Hit
ACTCTCGTTGCTCCTCTGGCATAGCCCCA10.16835016835016833No Hit
CCCACTCTCGTTGCTCCTCTGGCATAGCCC10.16835016835016833No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCACA10.16835016835016833No Hit
TTCGCGACATGGACCGAGGACCAAAAGAAAG10.16835016835016833No Hit
TCCGTGTTGTTCTACCCACTCTCGTTGCTCC10.16835016835016833No Hit
ATGCAGGAGGAGAAC10.16835016835016833No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGCAAG10.16835016835016833No Hit
TCTCGTTGCTCCTCTGGCATAGCCCC10.16835016835016833No Hit
CTGCCAGGCAAGTACCTACCTTTTGTTAGT10.16835016835016833No Hit
TTCTAACGCGTACTCCGTTAGTAGAGCTCC10.16835016835016833No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCATT10.16835016835016833No Hit
TAACAATGGTCAATCGCTAAAAGAACGCTGT10.16835016835016833No Hit
TGTGTTGTTCTACCAACTCTCGTTGCTCC10.16835016835016833No Hit
TCCATGCTATCCTGGGTTCCC10.16835016835016833No Hit
TCTAACGCGTACTCCGTTAGTAGAGCTCCAT10.16835016835016833No Hit
GCTGTGTTGTTCTACCCACTCTCGTTGCTC10.16835016835016833No Hit
ATAGGACGGAGCACCTGACCCTGCCAG10.16835016835016833No Hit
TAAAAGAACGCTGTGTTGTTCTACCCACTCTCG10.16835016835016833No Hit
ACAGTTAAGGGAGGACCAC10.16835016835016833No Hit
TGGTCAATCGCTAAAAGAACGCTGTGTCG10.16835016835016833No Hit
ATAGGACGGAGCACCTGACCCTGCCA10.16835016835016833No Hit
TCGTTGCTCCTCTGGCATAGCCCCACAGG10.16835016835016833No Hit
TACCCACTCTCGTTGCTCC10.16835016835016833No Hit
ACGCGTACTCCGTTAGTAGAGCTCCATGCTATCC10.16835016835016833No Hit
TAGGACGGAGCACCTGACCCTGCCAGGC10.16835016835016833No Hit
AGGACGGAGCACCTGACCCTGCCAGGCAC10.16835016835016833No Hit
CATCGTCAAGATCCTGCTCCCATTCACCT10.16835016835016833No Hit
TGAGGAACTAGGAGTTCACCCAGACATCGGGCC10.16835016835016833No Hit
TCAATCGCTAAAAGAACGCTGTGTTGTTTT10.16835016835016833No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCC10.16835016835016833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)