FastQCFastQC Report
Mon 16 Nov 2020
S34.15-35nt.28821.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.28821.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length15-30
%GC19

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAA1035.714285714285715No Hit
AAAAAAAAAAAAAAAAAAA310.714285714285714No Hit
ACTGGATTGAAAAAAAAAAAAAAAAA27.142857142857142No Hit
TAAAAAAAAAAAAAAAAAAAAA27.142857142857142No Hit
GTAAGTTCTGCCCCT27.142857142857142No Hit
AGACCTGAAGATCAAGGATGACCTCTTGCC27.142857142857142No Hit
AAAAAAAAAAAAAAAAAAAAA13.571428571428571No Hit
CAGGCTTAACTTTGCATGTTGATGGCTCC13.571428571428571No Hit
TTAAAAAAAAAAAAAAAAAAAAA13.571428571428571No Hit
TGGACTTGTATGTACGGGGCTGAGT13.571428571428571No Hit
AGATGGGTGTTCACCCAGACATTGGGCC13.571428571428571No Hit
AAAAAAAAAAAAAAAAAAAAAA13.571428571428571No Hit
TGAAGATCAAGGATGACCTCTTGCCAACC13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)