FastQCFastQC Report
Mon 16 Nov 2020
S34.15-35nt.28821.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.28821.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276
Sequences flagged as poor quality0
Sequence length15-33
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGAAGAACTCTCGTGCTTGCGCTGGG196.884057971014493No Hit
TAATCGCTGTACATCTCTCTGAACCGTGG124.3478260869565215No Hit
TTTCTTTACCAAGTACCCCTGAACCTTCA103.6231884057971016No Hit
TAGAACTCGTGAATCGCAGTGGGCTGCGG82.898550724637681No Hit
TAGTCTCTTCGCAGGAGCTGGACCGCCT82.898550724637681No Hit
TCGCTGTACATCTCTCTGAACCGTGGGA72.536231884057971No Hit
TTACCAAGTACCCCTGAACCTTCAGGCCCA62.1739130434782608No Hit
TACTAGTCTCTTCGCAGGAGCTGGACCGCC62.1739130434782608No Hit
TCTTTGAAGAACTCTCGTGCTTGCGCTGG62.1739130434782608No Hit
TGAAGAACTCTCGTGCTTGCGCTGGGGT51.8115942028985508No Hit
TGCGATATGGTTTTTCAAGGGAGATGGGC51.8115942028985508No Hit
TTGAAGAACTCTCGTGCTTGCGCTGGG51.8115942028985508No Hit
TACTCCAACCTCCTGAGAACATCTACGCATC41.4492753623188406No Hit
TCTTCCAACTTGACCTCCTCTGGTACTTGA41.4492753623188406No Hit
TACGTCTGTATGACTCAGCCCCGTACATA41.4492753623188406No Hit
TTACCAAGTACCCCTGAACCTTCAGGCCC41.4492753623188406No Hit
TACCAAGTACCCCTGAACCTTCAGGCCCA41.4492753623188406No Hit
TAGAACTCGTGAATCGCAGTGGGCTGCGGT31.0869565217391304No Hit
CATCTAGGGGCAGAACTTTTTTCATTAGA31.0869565217391304No Hit
TGAACACCCATCTCCTCTAGCAGCTTCCCGGC31.0869565217391304No Hit
TCAGCATCTACATCGATAACTACCCCGTC31.0869565217391304No Hit
TTTACCAAGTACCCCTGAACCTTCAGGCCCA31.0869565217391304No Hit
TACTAGTCTCTTCGCAGGAGCTGGACCGCCT31.0869565217391304No Hit
TGATCTTCCAACTTGACCTCCTCTGGTACT31.0869565217391304No Hit
TGAACACCCATCTCCTCTAGCAGCTTCCCGG31.0869565217391304No Hit
TTGAAGAACTCTCGTGCTTGCGCTGGGGT31.0869565217391304No Hit
TTGCTGTACATCTCTCTGAACCGTGGGA31.0869565217391304No Hit
TTTGAAGAACTCTCGTGCTTGCGCTGGGG31.0869565217391304No Hit
TCTACATCGATAACTACCCCGTCATCTGG31.0869565217391304No Hit
TACCAAGTACCCCTGAACCTTCAGGCCC31.0869565217391304No Hit
TAGAACTCGTGAATCGCAGTGGCTGC31.0869565217391304No Hit
TGATCTTCCAACTTGACCTCCTCTGGTACTT31.0869565217391304No Hit
TGATTAACCTACCATTCTGAGACAGTTGAT31.0869565217391304No Hit
TCCAATAGAACTCGTGAATCGCAGTGGGC31.0869565217391304No Hit
TAGAACTCGTGAATCGCAGTGGGCTGC31.0869565217391304No Hit
TCATGGTACCGCTCGGCTCAACGATCTT20.7246376811594203No Hit
ATAGAACTCGTGAATCGCAGTGGGCTGC20.7246376811594203No Hit
TCTTTTCCAGGCCTTGCATCAATGATGCT20.7246376811594203No Hit
TGAAGAACTCTCGTGCTTGCGCTGGGG20.7246376811594203No Hit
TGCGCTAGGTACTAGTCTCTTCGCAGG20.7246376811594203No Hit
TGAACACCCATCTCCTCTAGCAGCTTCCCGC20.7246376811594203No Hit
TTAGCAGTCATGGTACCGCTCGGCTCAACG20.7246376811594203No Hit
TGGGTGAACACCCATCTCCTCTAGCAGCTTC20.7246376811594203No Hit
TACCAAGTACCCCTGAACCTTCAGGCCCAAT20.7246376811594203No Hit
TTCACGATATCAACTTGTCCCTTTGACACCGG20.7246376811594203No Hit
TTTCTTTACCAAGTACCCCTGAACCTTCAGGCC20.7246376811594203No Hit
TTCGAGCAAACATCTAGGGGCAGAACTT20.7246376811594203No Hit
TTGAAGAACTCTCGTGCTTGCGCTGGGG20.7246376811594203No Hit
TGAACATCGACATGT20.7246376811594203No Hit
TTTGAAGAACTCTCGTGCTTGCGCTGG20.7246376811594203No Hit
TCTTTGAAGAACTCTCGTGCTTGCGCTGGG20.7246376811594203No Hit
TGAAGAACTCTCGTGCTTGCGCTGG20.7246376811594203No Hit
TCCAGATGTCGAGATGAACAACTCTTTGTC20.7246376811594203No Hit
TGTCCCTTTGACACCGGACTTAATGCGTC20.7246376811594203No Hit
TCCGATACCCAGGTGATCTTCTTCTGAT10.36231884057971014No Hit
TTTGAAGAACTCTCGTGCTTGCGCCGGG10.36231884057971014No Hit
TTTCTTTACCAAGTACCACTGAACCTTCA10.36231884057971014No Hit
TCCAACCTCCTGAGAACATCTACGCATCTT10.36231884057971014No Hit
AGGTTGCATCTTTTCCAGGCCTTGCAT10.36231884057971014No Hit
TGAAGAACTCTCGTGCTTGCGCTGGG10.36231884057971014No Hit
TGCGCTAGGTACTAGTCTCTTCGCAG10.36231884057971014No Hit
TTTGTATCCAGATGTCGAGATGAACAACT10.36231884057971014No Hit
TCATTAGAGGACCAAAGGCTGTCTCATCT10.36231884057971014No Hit
TCAGCATCTACATCGATAACTACCCCGTCA10.36231884057971014No Hit
TAGAACTCGTGAATCGCAGTGGGCTG10.36231884057971014No Hit
CTGGACCGCCTCTACTCTCGACAGCATAGGT10.36231884057971014No Hit
TAATGCGTCTGGTTTATTGTTGTAAAAGTT10.36231884057971014No Hit
TCACGATATCAACTTGTCCCTTTGACACCG10.36231884057971014No Hit
TACTCCAACCTCCGGAGAACATCTACGCATC10.36231884057971014No Hit
GGGCAGAACTTTTTTCATTAGAGGACC10.36231884057971014No Hit
TGTATCCAGATGTCGAGATGAACAACTCT10.36231884057971014No Hit
TCCAACCTCCTGAGAACATCTACGCATCTTT10.36231884057971014No Hit
GAACACCCATCTCCTCTAGCAGCTTCCCGGC10.36231884057971014No Hit
TTTGTATCCAGATGTCGAGATGAACAACTC10.36231884057971014No Hit
CTTCCAACTTGACCTCCTCTGGTACTTGA10.36231884057971014No Hit
TCCAACCTCCTGAGAACATCTACGCATCT10.36231884057971014No Hit
TCGAGATGAACAACTCTTTGTCGGAG10.36231884057971014No Hit
ACTAGTCTCTTCGCAGGAGCTGGACCGCCT10.36231884057971014No Hit
TGTATCCAGATGTCGAGATGAACAACTCTTT10.36231884057971014No Hit
TATGACTCAGCCCCGTACATACAAGTCCAT10.36231884057971014No Hit
TTCATACGTCTGTATGACTCAGCCCCGT10.36231884057971014No Hit
TTTAACTTGCCCTGCTTCTCTCACAGACACAG10.36231884057971014No Hit
TTTGGACCGCCTCTA10.36231884057971014No Hit
ATCGCTGTACATCTCTCTGAACCGTGG10.36231884057971014No Hit
TGTCGCAAACCCTCTTTCTCCTCCAATAGGTC10.36231884057971014No Hit
TCTGGTCGTAAACAAAAAGTCCGTCTCCC10.36231884057971014No Hit
TAAGACTTTCACGATATCAACTTGTCCCTT10.36231884057971014No Hit
TCGAGCAAACATCTAGGGGCAGAACTTTT10.36231884057971014No Hit
GAGGACCAAAGGCTGTCTCATCTGCGATAGG10.36231884057971014No Hit
TAGAGGACCAAAGGCTGTCTCATCTGCGA10.36231884057971014No Hit
TTCACGATATCAACTTGTCCCTTTGACACCG10.36231884057971014No Hit
TGTCAGTGGCGAGCCTGATTCATACGTC10.36231884057971014No Hit
TCCCACCACCATCTACTCCAACCTCCTGAG10.36231884057971014No Hit
TGTTAGCAGTCATGGTACCGCTCGGCT10.36231884057971014No Hit
TTCAAGGGAGATGGGCTATTCACTAGGCA10.36231884057971014No Hit
TGTCGAGATGAACAACTCTTTGTCGGAGCC10.36231884057971014No Hit
CTCCAACCTCCGGAGAACATCTAC10.36231884057971014No Hit
ATAGAACTCGTGAATCGCAGTGGGC10.36231884057971014No Hit
TGCGATATGGTTTTTCAAGGGAGATGG10.36231884057971014No Hit
TACTCCAACCTCCTGAGAACATCTACGCAT10.36231884057971014No Hit
TGATTGTTAGCAGTCATGGTACCGCTCGGC10.36231884057971014No Hit
TTTGTCAAGTGGCGAGCCTGATTCATACGT10.36231884057971014No Hit
TCATTTATTCCTAGGACCTCTTTAGCTT10.36231884057971014No Hit
TCCAGATGTCGAGATGAACAACTCTTTGT10.36231884057971014No Hit
ATAGAACTCGTGAATCGCAGTGGGCTGCGGT10.36231884057971014No Hit
TACGTCTGTATGACTCAGCCCCGTACATAC10.36231884057971014No Hit
TTCCAACTTGACCTCCTCTGGTACTTGAGGC10.36231884057971014No Hit
TAGTCTCTTCGCAGGAGCTGGACCGCC10.36231884057971014No Hit
TCCAACTTGACCTCCTCTGGTACTTGAGGC10.36231884057971014No Hit
TAGAGGACCAAAGGCTGTCTCATCTGCGAA10.36231884057971014No Hit
TCTGTCCATGTCGCAAACCCTCTTTCTCCT10.36231884057971014No Hit
TGTATCCAGATGTCGAGATGAACAACTC10.36231884057971014No Hit
TCCATGTCGCAAACCCTCTTTCTCCTCCA10.36231884057971014No Hit
TGCTAGCTCTGCCACCAGAGTAGTTGCGGC10.36231884057971014No Hit
TCTACATCGATAACTACCCCGTCATATGG10.36231884057971014No Hit
TCTAGGGGCAGAACTTTTTTCATTAGAGGAC10.36231884057971014No Hit
TCTGCGATATGGTTTTTCAAGGGAGATGGG10.36231884057971014No Hit
TTTCTTTACCAAGTACCCCCGAACCTTCA10.36231884057971014No Hit
TGACTCAGCCCCGTACATACAAGTCCATG10.36231884057971014No Hit
TCGGAGCCATCAACATGCAAAGTTAAGCCT10.36231884057971014No Hit
TCAACATGCAAAGTTAAGCCTGGCTCAAGC10.36231884057971014No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)