FastQCFastQC Report
Mon 30 Nov 2020
S34.15-35nt.2997.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.2997.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70
Sequences flagged as poor quality0
Sequence length15-34
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTGAAGAAACTGTGCCCGGAGA912.857142857142856No Hit
CCCCTGTGAAGAAACTGTGCCCGGAGA68.571428571428571No Hit
GTCCGACGGCCCCTG57.142857142857142No Hit
CCTGTGAAGAAACTGTGCCCGGAGAA45.714285714285714No Hit
TTACAAGATCGAGGCCCCCACATCTCACT34.285714285714286No Hit
TTACATAAAATTACAAGATCGAGGCCCC34.285714285714286No Hit
TCATCGCAGAACACCACGACCACGTCCACG34.285714285714286No Hit
TTCATCGAGCAGCAGCGTGGTCGCGTCAGC34.285714285714286No Hit
CATCGCAGAACACCACGACCACGTCCACG22.857142857142857No Hit
TATTACATAAAATTACAAGATCGAGGCCCC22.857142857142857No Hit
TCGAGGCCCCCACATCTCACTTTCTGGCT22.857142857142857No Hit
CCTGTGAAGAAACTGTGCCCGGAG22.857142857142857No Hit
CCCCTGTGAAGAAACTGTGCCCGGAGAA22.857142857142857No Hit
TCCGGAGCCAAAGTGCAAGTGCATTTGACT11.4285714285714286No Hit
CCCCTGTGAAGAAACTGTGCCCGGAGAAAGC11.4285714285714286No Hit
CCTGTGAAGAAACTGTGCCCGGAGAAAGC11.4285714285714286No Hit
TAAATCCTAGTTCTAGACCGCCACAAAGA11.4285714285714286No Hit
CCCCTGTGAAGAAACTGTGCCCGGAG11.4285714285714286No Hit
TACAAGATCGAGGCCCCCACATCTCACT11.4285714285714286No Hit
AGACCGACGGCCCCTGT11.4285714285714286No Hit
CCTGTGAAGAAACTGTGCCCGGA11.4285714285714286No Hit
GAGATTACTACGCAACAATTGACCCTG11.4285714285714286No Hit
AAGAAACTGTGCCCGGAGA11.4285714285714286No Hit
TCTAGACCGCCACAAAGAACTTTACTACAG11.4285714285714286No Hit
TATTCCCCGGCATGAATTCACAATTAAGT11.4285714285714286No Hit
CCTGTGAAGAAACTGTGCCCGGAGAAT11.4285714285714286No Hit
TTTGCCGGAAAACCGAATATAGAACCAAA11.4285714285714286No Hit
AGGAAGTCTACTGGAAGCAAGCAACT11.4285714285714286No Hit
CCCTGTGAAGAAACTGTGCCCGGAGA11.4285714285714286No Hit
CTGTGAAGAAACTGTGCCCGGAGAAAGC11.4285714285714286No Hit
GACTATAGGTCTGCTGCCCCCACTATT11.4285714285714286No Hit
TCACATGAAAGAGTCGTTCCCGAATACTC11.4285714285714286No Hit
CCTGTGAAGAAACTGTGCCCGGAGAACC11.4285714285714286No Hit
AACACCACGACCACGTCCACGTTATACACGACTG11.4285714285714286No Hit
TCCCCTGTGAAGAAACTGTGCCCGGAG11.4285714285714286No Hit
GACAGTGCAGATGTG11.4285714285714286No Hit
CCGACGGCCCCTGTGAAGAAACTGTGCC11.4285714285714286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)