FastQCFastQC Report
Mon 30 Nov 2020
S34.15-35nt.2997.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.2997.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2997
Sequences flagged as poor quality0
Sequence length15-35
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCCGTCTGCGCACTGGATGAAACCGT1755.839172505839173No Hit
TTCTTGAAGTCCCGAGTTATCGCTTTCTG1434.7714381047714385No Hit
TCCCGAGTTATCGCTTTCTGTAATTGTCTC792.6359693026359694No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACATC652.1688355021688355No Hit
TACTTAATTGTGAACTCATGCCGGGGAA642.135468802135469No Hit
TAACTCTTCGTCCGACTCGACATCCATA591.9686353019686353No Hit
TGCTTCCAGTAGACTTCCTTCGGTTGACCT551.8351685018351684No Hit
TCTTGAAGTCCCGAGTTATCGCTTTCTG541.8018018018018018No Hit
TCCATAATTGGCGGTTTGCTGGTAGATA511.7017017017017018No Hit
TTCAACGGCGCAGCAGGGTGGTTCCGAAC491.6349683016349683No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACA461.534868201534868No Hit
TCGTGTATAACGTGGACGTGGTCGTGGT361.2012012012012012No Hit
TAGATATGGGCACATCTGCACTGGTTGGAT351.1678345011678346No Hit
TCGTGTATAACGTGGACGTGGTCGTGGTG351.1678345011678346No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGAGT331.1011011011011012No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGAG311.0343677010343677No Hit
TCCTCCCATACTTGCGCTTGCTGATCGGG270.9009009009009009No Hit
TCCGGGCACAGTTTCTTCACAGGGGACGTC250.8341675008341675No Hit
TCATCTCCAGATTCGCATGCTGACGCGAC240.8008008008008007No Hit
TGCCCCGTCTGCGCACTGGATGAAACC240.8008008008008007No Hit
TACTTAATTGTGAACTCATGCCGGGGAAT230.767434100767434No Hit
TGCCCCGTCTGCGCACTGGATGAAACCG230.767434100767434No Hit
TGCTTGCTTCCAGTAGACTTCCTTCGGT220.7340674007340674No Hit
TAATTGGCGGTTTGCTGGTAGATATGGGC220.7340674007340674No Hit
TTTGCTGGTAGATATGGGCACATCTGCACTG220.7340674007340674No Hit
TGCTGATCGGGAGTCAAATGCACTTGCACT200.667334000667334No Hit
TCTCCATAATCCATTAGTGACCAAGCCGCC180.6006006006006006No Hit
TTCTGTAATTGTCTCAACAGTTGCCCCGTC180.6006006006006006No Hit
TATGGGCACATCTGCACTGGTTGGATT170.5672339005672339No Hit
TGCTGGTAGATATGGGCACATCTGCACT160.5338672005338673No Hit
TTGCCCCGTCTGCGCACTGGATGAAACCG160.5338672005338673No Hit
TTCACAGGGGCCGTCGGTCTGGGACACTC160.5338672005338673No Hit
TGACCAAGCCGCCAATTCAATTGCTCTGTT160.5338672005338673No Hit
TAAGACTGTAGTAAAGTTCTTTGTGGCGGT140.46713380046713376No Hit
TTGCCCCGTCTGCGCACTGGATGAAACCGT140.46713380046713376No Hit
TGAAGTCCCGAGTTATCGCTTTCTGTAATT140.46713380046713376No Hit
TATGGGCACATCTGCACTGGTTGGATTG130.43376710043376715No Hit
TGAAATATGAGTTTCAACGGCGCAGCAGGG130.43376710043376715No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACAT130.43376710043376715No Hit
TGTCTCAACAGTTGCCCCGTCTGCGCACT130.43376710043376715No Hit
TCTGTAATTGTCTCAACAGTTGCCCCGTC130.43376710043376715No Hit
TAGTAATTGTTCTCCCGTATTTCTGGGGC120.40040040040040037No Hit
TTGGCGGTTTGCTGGTAGATATGGGCAC120.40040040040040037No Hit
TGCTTGCTTCCAGTAGACTTCCTTCGGTT110.3670337003670337No Hit
TCACAGGGGCCGTCGGTCTGGGACACTC110.3670337003670337No Hit
TGCTTCCAGTAGACTTCCTTCGGTTGACC110.3670337003670337No Hit
TCGTCCGACTCGACATCCATAATTGGCGGT110.3670337003670337No Hit
TTCGAGCTTCATTACCAAATCGTGGGAT100.333667000333667No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGA100.333667000333667No Hit
TCCGGGCACAGTTTCTTCACAGGGGCCGTC100.333667000333667No Hit
TCGACATCCATAATTGGCGGTTTGCTGGT100.333667000333667No Hit
TTCGTCCGACTCGACATCCATAATTGGCGGT90.3003003003003003No Hit
TGCTGGTAGATATGGGCACATCTGCACTG90.3003003003003003No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTGA90.3003003003003003No Hit
TATGAGCTTCAACGGCGCAGCAGGGTGG90.3003003003003003No Hit
TTGTCTCAACAGTTGCCCCGTCTGCGCAC90.3003003003003003No Hit
TCATCTCCAGATTCGCATGCTGACGCGACC90.3003003003003003No Hit
TTCGAGCTTCATTACCAAATCGTGGGATC90.3003003003003003No Hit
TATGGGCACATCTGCACTGGTTGGATTGAT90.3003003003003003No Hit
TTTGCTGGTAGATATGGGCACATCTGCACT80.26693360026693363No Hit
TCACAGGGGACGTCGGTCTGGGACACTC80.26693360026693363No Hit
TTGTAGATCGTCTGGAACTTGGTAAGAGA80.26693360026693363No Hit
TCTTCACAGGGGACGTCGGTCTGGGACACT80.26693360026693363No Hit
TCGGAAGAAGAGCATCCCATACTTGTTGAGT80.26693360026693363No Hit
TAAGTCTTCGTCCGACTCGACATCCATA80.26693360026693363No Hit
TTCCTCCCATACTTGCGCTTGCTGATCGG80.26693360026693363No Hit
TGCTGGTAGATATGGGCACATCTGCACTGG80.26693360026693363No Hit
TCTTCACAGGGGACGTCGGTCTGGGACACTC80.26693360026693363No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTG80.26693360026693363No Hit
TCCGAACGAACTCGAGTAATTGTTCTCCCG70.23356690023356688No Hit
TTGGCTTTCGAAGGAGACGGATGTGTGCC70.23356690023356688No Hit
TGCGCTTGCTGATCGGGAGTCAAATGCACT70.23356690023356688No Hit
TACTTAATTGTGAACTCATGCCGGGGA70.23356690023356688No Hit
TCTCAACAGTTGCCCCGTCTGCGCACTGG70.23356690023356688No Hit
TCGACATCCATAATTGGCGGTTTGCTGGTA70.23356690023356688No Hit
TAGATATGGGCACATCTGCACTGGTTGGA70.23356690023356688No Hit
TCTTCACAGGGGCCGTCGGTCTGGGACACTC70.23356690023356688No Hit
TCCGGGCACAGTTTCTTCACAGGGGACG70.23356690023356688No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTT70.23356690023356688No Hit
ACTGCCCATCTCGCCTTTCTTGAAGTCCCG60.20020020020020018No Hit
TATCGCTTTCTGTAATTGTCTCAACAGTT60.20020020020020018No Hit
TCCTTCGGTTGACCTACACATTTCGAGTA60.20020020020020018No Hit
TCTTGAAGTCCCGAGTTATCGCTTTCTGT60.20020020020020018No Hit
TTGTCTCAACAGTTGCCCCGTCTGCGCACT60.20020020020020018No Hit
TCGGTCTGGGACACTCTTGGAAGGTCAGC60.20020020020020018No Hit
TACTTAATTGTGAATTCATGCCGGGGAA60.20020020020020018No Hit
TCCAAGTTGCTTGCTTCCAGTAGACTTCCT60.20020020020020018No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACT60.20020020020020018No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCG60.20020020020020018No Hit
TAGTGACCAAGCCGCCAATTCAAT60.20020020020020018No Hit
TCCGGGCACAGTTTCTTCACAGGGGCCGT60.20020020020020018No Hit
TGTTGTAGATCGTCTGGAACTTGGTAAGA60.20020020020020018No Hit
TTCTTCACAGGGGCCGTCGGTCTGGGACA60.20020020020020018No Hit
TGTTCCTCCCATACTTGCGCTTGCTGATCGG50.1668335001668335No Hit
TCTGCACTGGTTGGATTGATAGGAACAGTC50.1668335001668335No Hit
TCTCCATAATCCATTAGTGACCAAGCCGCCT50.1668335001668335No Hit
TCTTCACAGGGGCCGTCGGTCTGGGACACT50.1668335001668335No Hit
TATATTGTTGTAGATCGTCTGGAACTTGGT50.1668335001668335No Hit
TACAAGCTGCTCGTCCCGTACTTTGTAACG50.1668335001668335No Hit
TTCATCTCCAGATTCGCATGCTGACGCGAC50.1668335001668335No Hit
TATTGTTGTAGATCGTCTGGAACTTGGT50.1668335001668335No Hit
TTCGAGCTTCATTACCAAATCGTGGGATCGA50.1668335001668335No Hit
TAATTGTCTCAACAGTTGCCCCGTCTGC50.1668335001668335No Hit
TATAACGTGGACGTGGTCGTGGTGTTCTG50.1668335001668335No Hit
TGAAAAGCCATGAAGGACCAATTGGTATAGT50.1668335001668335No Hit
TGTCTCAACAGTTGCCCCGTCTGCGCACTG50.1668335001668335No Hit
TCCCGAGTTATCGCTTTCTGTAATTGTCTCAAC50.1668335001668335No Hit
TCATATCGCACCCCTATTTCGACACTGCC50.1668335001668335No Hit
TCACAGGGGCCGTCGGTCTGGGACACTCT50.1668335001668335No Hit
TGTGCCGAACCGACGTAAGTTCTGTTGTGG50.1668335001668335No Hit
TGAAATATGAGCTTCAACGGCGCAGCAGGG50.1668335001668335No Hit
TTCCTCTTCGCCTTCTTCATAGTTGTCGG50.1668335001668335No Hit
TCCTTCGGTTGACCTACACATTTCGAGTAA50.1668335001668335No Hit
TCCTTCGGTTGACCTACACATTTCGAGT50.1668335001668335No Hit
CTTCCTTCGGTTGACCTACACATTTCGAGTA50.1668335001668335No Hit
TGGGCACATCTGCACTGGTTGGATTGATAG50.1668335001668335No Hit
TACAGTTTGAAGAATTCTAGCACCACAAT50.1668335001668335No Hit
TGTTGTAGATCGTCTGGAACTTGGTAAGAG50.1668335001668335No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTC50.1668335001668335No Hit
TTCGAAGGAGACGGATGTGTGCCGAACC40.13346680013346682No Hit
TACTTGCGCTTGCTGATCGGGAGTCAAAT40.13346680013346682No Hit
TATAACGTGGACGTGGTCGTGGTGTTCTGC40.13346680013346682No Hit
TCTTCGTCCGACTCGACATCCATAATTGGC40.13346680013346682No Hit
TCCGAACGAACTCGAGTAATTGTTCTCCC40.13346680013346682No Hit
TAGTGGGGGCAGCAGACCTATAGTCACGC40.13346680013346682No Hit
TAGATATGGGCACATCTGCACTGGTTGG40.13346680013346682No Hit
TGCTCGATGAAATAGTGGGGGCAGCAGACCT40.13346680013346682No Hit
ACTGCCCATCTCGCCTTTCTTGAAGTCCCGA40.13346680013346682No Hit
TTGCTGGTAGATATGGGCACATCTGCACT40.13346680013346682No Hit
TTTGCTGGTAGATATGGGCACATCTGCAC40.13346680013346682No Hit
TTCTCCGGGCACAGTTTCTTCACAGGGGCC40.13346680013346682No Hit
TAATTGGCGGTTTGCTGGTAGATATGGGCACA40.13346680013346682No Hit
TAAGCACACAGTAAGTTTATAAAAAGACTTGAAC40.13346680013346682No Hit
TTAAACTGCCCATCTCGCCTTTCTTGAAG40.13346680013346682No Hit
TTCACAGGGGACGTCGGTCTGGGACACTC40.13346680013346682No Hit
TCCGAACGAACTCGAGTAATTGTTCTCCCGT40.13346680013346682No Hit
TAAACTGCCCATCTCGCCTTTCTTGAAGT40.13346680013346682No Hit
TCGCTTTCTGTAATTGTCTCAACAGTT40.13346680013346682No Hit
TCGTGTATAACGTGGACGTGGTCGTGG40.13346680013346682No Hit
TGGTTGGATTGATAGGAACAGTCGTGTAT40.13346680013346682No Hit
TCGTGTATAACGTGGATGTGGTCGTGGT40.13346680013346682No Hit
ATACTTAATTGTGAACTCATGCCGGGGAA40.13346680013346682No Hit
CTCGACATCCATAATTGGCGGTTTGCTGGT40.13346680013346682No Hit
CCCCGTCTGCGCACTGGATGAAACCGT40.13346680013346682No Hit
TTGTGAACTCATGCCGGGGAATAACTCTTC40.13346680013346682No Hit
TTCTGAAATTGTCTCAACAGTTGCCCCGTC40.13346680013346682No Hit
TGAGTACAGTTTGAAGAATTCTAGCACCAC40.13346680013346682No Hit
TCCATAATCCATTAGTGACCAAGCCGCCAAT40.13346680013346682No Hit
TGTAGATCGTCTGGAACTTGGTAAGAGAT40.13346680013346682No Hit
TGCGCTATTTCGGCCCATAACATGGACAT40.13346680013346682No Hit
TCGCATGCTGACGCGACCACGCTGCTGC40.13346680013346682No Hit
AGTTTGAAGAATTCTAGCACCACGATGAA40.13346680013346682No Hit
TACTTAATTGTGAATTCATGCCGGGGAAT30.10010010010010009No Hit
TTGGATTGATAGGAACAGTCGTGTATAACGT30.10010010010010009No Hit
CTCGACATCCATAATTGGCGGTTTGCTG30.10010010010010009No Hit
CATACTTAATTGTGAATTCATGCCGGGG30.10010010010010009No Hit
TAGGAACAGTCGTGTATAACGTGGACGTG30.10010010010010009No Hit
TCCGGGCACAGTTTCTTCACAGGGGACGT30.10010010010010009No Hit
TTGACGGCATACTTAATTGTGAACTCATGC30.10010010010010009No Hit
TCAACGGCGCAGCAGGGTGGTTCCGAACGA30.10010010010010009No Hit
TAGTGGGGGCAGCAGACCTATAGTCACGCC30.10010010010010009No Hit
TTCACAGGGGCCGTCGGTCTGGGACACTCTT30.10010010010010009No Hit
TCGGAAGAAGAGCATCCCATACTTGTTGAG30.10010010010010009No Hit
TATGTATTTGATGCGCTATTTCGGCCCAT30.10010010010010009No Hit
TGCGTAGTAATCTCCATAATCCATTAGTG30.10010010010010009No Hit
TCCATAATCCATTAGTGACCAAGCCGCCAA30.10010010010010009No Hit
TCGAAGGAGACGGATGTGTGCCGAACCGACT30.10010010010010009No Hit
TGTTGAGTACAGTTTGAAGAATTCTAGCAC30.10010010010010009No Hit
TTCCAGTAGACTTCCTTCGGTTGACCTA30.10010010010010009No Hit
TTGCTTCCAGTAGACTTCCTTCGGTTGACT30.10010010010010009No Hit
TGGTAGATATGGGCACATCTGCACTGGTT30.10010010010010009No Hit
TCTTGGTCCTTTTTCCAAATAGAGCACGG30.10010010010010009No Hit
TGAAACCGTGCACAATGTCTTCCAAGTTGC30.10010010010010009No Hit
TGTGTGCCGAACCGACGTAAGTTCTGTT30.10010010010010009No Hit
TCTGCACTGGTTGGATTGATAGGAACAGT30.10010010010010009No Hit
TGCCCCGTCTGCGCACTGGA30.10010010010010009No Hit
TGCGCTTGCTGATCGGGAGTCAAATGC30.10010010010010009No Hit
TTCTTCACAGGGGCCGTCGGTCTGGGAC30.10010010010010009No Hit
TCCGAACGAACTCTAGTAATTGTTCTCCCG30.10010010010010009No Hit
TATCGCACCCCTATTTCGACACTGCCATATC30.10010010010010009No Hit
TTTGCTGGTAGATATGGGCACATCTGCACTGC30.10010010010010009No Hit
TCTGGGACACTCTTGGAAGGTCAGCTTCCT30.10010010010010009No Hit
TATGTACATACGGACGGACCCTTTACTACC30.10010010010010009No Hit
TCACAGGGGCCGTCGGTCTGGGACACTCTT30.10010010010010009No Hit
TAATTGTGAACTCATGCCGGGGAATAACT30.10010010010010009No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTGCATT30.10010010010010009No Hit
TTAGTGACCAAGCCGCCAATTCAATTGCTCT30.10010010010010009No Hit
TTAAGCACACAGTAAGTTTATAAAAAGACT30.10010010010010009No Hit
TCCATAATTGGCGGTTTGCTGGTAGATATG30.10010010010010009No Hit
TCTTCATAGTTGTCGGAAGTTGTCGGAAG30.10010010010010009No Hit
TTCAACGGCGCAGCAGGGTGGTTCCGAACT30.10010010010010009No Hit
TGGTCCAGTGAGGCGTTTGAAATATGAG30.10010010010010009No Hit
TTAATTGTGAACTCATGCCGGGGAATAAC30.10010010010010009No Hit
CTCGACATCCATAATTGGCGGTTTGCTGGTA30.10010010010010009No Hit
TGACATTTTCTTCATCTCCAGATTCGCAT30.10010010010010009No Hit
TTGGTATAGTCGGCTTCTTTCATAATTAT30.10010010010010009No Hit
TGTCTCAACAGTTGCCCCGTCTGCGCAC30.10010010010010009No Hit
TGTAGATCGTCTGGAACTTGGTAAGAG30.10010010010010009No Hit
TATTATATTACAAGCTGTTCGTCCCGTAC30.10010010010010009No Hit
TGCTGATCGGGAGTCAAATGCACTTGCACTT30.10010010010010009No Hit
TCCAGATTCGCATGCTGACGCGACCACGC30.10010010010010009No Hit
TCCATTAGTGACCAAGCCGCCAATTCAAT30.10010010010010009No Hit
AGTTTCTTCACAGGGGACGTCGGTCTGG30.10010010010010009No Hit
TTCATCTCCAGATTCGCATGCTGACGCGACC30.10010010010010009No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTTT30.10010010010010009No Hit
TTCTTCACAGGGGCCGTCGGTCTGGGACAC30.10010010010010009No Hit
TTCTTGAAGTCCCGAGTTATCGCTTTCT30.10010010010010009No Hit
TTCTTCACAGGGGCCGTCGGTCTGGG30.10010010010010009No Hit
TTGGTCCAGTGAGGCGTTTGAAATATGAGC30.10010010010010009No Hit
TGGTGTTCTGCGATGACGGTAAATGCGT30.10010010010010009No Hit
TGTATAACGTGGACGTGGTCGTGGTGTT30.10010010010010009No Hit
TTAATTGTGAACTCATGCCGGGGAATAACT30.10010010010010009No Hit
TGAGTACAGTTTGAAGAATTCTAGCACCA30.10010010010010009No Hit
TAATTGGCGGTTTGCTGGTAGATATGGG30.10010010010010009No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTGAA30.10010010010010009No Hit
TCAAATGCACTTGCACTTTGGCTCCGGAT30.10010010010010009No Hit
CCGGGGAATAACTCTTCGTCCGACTCGACA30.10010010010010009No Hit
TACTTAATTGTGAATTCATGCCGGGGA30.10010010010010009No Hit
TAAACTGCCCATCTCGCCTTTCTTGAAGTC30.10010010010010009No Hit
TGTAAAACGTTTGCTTTGACAGGTATCCT30.10010010010010009No Hit
ACAGTTTGAAGAATTCTAGCACCACGATG30.10010010010010009No Hit
TTAAACTGCCCATCTCGCCTTTCTTGAAGT30.10010010010010009No Hit
TTCGTCCGACTCGACATCCATAATTGGCGG30.10010010010010009No Hit
TACAAGCTGTTCGTCCCGTACTTTGTAAC30.10010010010010009No Hit
TTCGCATGCTGACGCGACCACGCTGCTGC30.10010010010010009No Hit
TTGTTGTAGATCGTCTGGAACTTGGTAAG30.10010010010010009No Hit
TTAGTGACCAAGCCGCCAATTCAATTGCTC30.10010010010010009No Hit
TTTTCTTCATCTCCAGATTCGCATGCTGAC30.10010010010010009No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTGCACC30.10010010010010009No Hit
TCGTGTATAACGTGGACGTGGTCG30.10010010010010009No Hit
TCTCCATAATCCATTAGTGACCAAGCCGCCAAT30.10010010010010009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)