FastQCFastQC Report
Mon 30 Nov 2020
S34.15-35nt.31032.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.31032.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119
Sequences flagged as poor quality0
Sequence length16-33
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAAGCTGCCCGTCCCGTACTTTGTAACA75.88235294117647No Hit
TACAAGCTGCCCGTCCCGTACTTTGTAACG65.042016806722689No Hit
TCACTTTCGATTTCTTTCCCGTGCTCCGT43.361344537815126No Hit
ACATCGGCCCTCACAATTTAGCCACC32.5210084033613445No Hit
AAGCTGCCCGTCCCGTACTTTGTAACG32.5210084033613445No Hit
ACGAGCTGCCCGTCCCGTACTTTGTAAC32.5210084033613445No Hit
AAGCTGCCCGTCCCGTACTCTGTAACGGA32.5210084033613445No Hit
ACAAGCTGCCCGTCCCG32.5210084033613445No Hit
TCAACAAGCTGCCCGTCCCGTACTTTGTAA21.680672268907563No Hit
GTTTACAAGCTGCCCGTCCCGTACT21.680672268907563No Hit
CTAAATAACAAGCTGCCCGTCCCGTACT21.680672268907563No Hit
CCTTGGGGTTACCAAGCCGCGCCTTACCACCC21.680672268907563No Hit
TTGGTACTGAAAGGGTTGGCAGTTGGAA21.680672268907563No Hit
GTTACCAAGCCGCGCCTTACCACACT21.680672268907563No Hit
TACCAAGCCGCGCCTTACCACCCGA21.680672268907563No Hit
TAGACATGTATGACCTCCCCTCGGACGACA21.680672268907563No Hit
TAGACATGTATGACCTCCCCTCGGACGAC21.680672268907563No Hit
TTGGCAGTTGGAAATGTAACGTCTGATT21.680672268907563No Hit
TTGGTACTGAAAGGGTTGGCAGTTGGA21.680672268907563No Hit
TACCAAGCCGCGCCTTACCAC21.680672268907563No Hit
TACCAAGCCGCGCCTTACCACCCTAG21.680672268907563No Hit
AAGCTGCCCGTCCCGTACTTTGTAAC21.680672268907563No Hit
AGCTGCCCGTCCCGTACTTTG21.680672268907563No Hit
TTACCAAGCCGCGCCTTACCACCCTAGCT21.680672268907563No Hit
TACAAGCTGCCCGTCCCGTACTTTGTAACAG21.680672268907563No Hit
TTGGCAGTTGGAAATGTAACGTCTGATTTGGA21.680672268907563No Hit
AGCTGCCCGTCCCGTACTTTGTAAC21.680672268907563No Hit
TAACAAGCCGCCCGTCCCGTACTTTG10.8403361344537815No Hit
AAAGTAGCTCGGTGCGGGGTTCGAACCCG10.8403361344537815No Hit
TTACCAAGCCGCGCCTTACCACTCTAGCTAC10.8403361344537815No Hit
TGCCCGTCCCGTACTTTGTAAC10.8403361344537815No Hit
ACCGCGCCTTACCACCC10.8403361344537815No Hit
TTACCAAGCCGCGCCTTACCACCCTAGG10.8403361344537815No Hit
TTGGTACTGAAAGGGATGGCAGTTGGA10.8403361344537815No Hit
CCAAGCCGCGCCTTACCACCCGA10.8403361344537815No Hit
AAGCTGCCCGTCCCGTACTTTGT10.8403361344537815No Hit
CAAGCTGCCCGTCCCGTACTTTGTAACAGAGA10.8403361344537815No Hit
TACCAAGCCGCGCCTTACCACCCGAG10.8403361344537815No Hit
GCCGCGCCTTACCACCC10.8403361344537815No Hit
CAAGCTGCTCGTCCCGTACTTTGTAA10.8403361344537815No Hit
AGCTGCCCGTCCCGTACTTTGTAACGCA10.8403361344537815No Hit
TTTTACTTTACTCCCTTGGTACTGAAAGG10.8403361344537815No Hit
TAACGGAGAAAAAAAAGTAGCCCGGTGCGGG10.8403361344537815No Hit
CAAGCTGCCCGTCCCGTACTTTGTAAC10.8403361344537815No Hit
ATACAAGCTGCCCGTCCCGTA10.8403361344537815No Hit
AACAAGCTGCCCGTCCCGTACT10.8403361344537815No Hit
CAAGCTGCCCGTCCCGTACTTTGTAACGG10.8403361344537815No Hit
TACAAGCTGCCCGTCCCGTACTTTGTA10.8403361344537815No Hit
TAGGGTTACCAAGCCGCGCCTTACCACCC10.8403361344537815No Hit
TAGCCCGGTGTGGGGTTCGAACCCGTGACC10.8403361344537815No Hit
TACAAGCTGCCCGTCCCGTACTTTGTAA10.8403361344537815No Hit
TGGCAGTTGGAAATGTAACGTCTGATTCG10.8403361344537815No Hit
TCTTCTACAGACCCGGTTTGCTCGAATTTT10.8403361344537815No Hit
TTACCAAGCCGCGCCTTACCACCCTAGCTA10.8403361344537815No Hit
TTGGTACTGAAAGGGTTGGCAGTTGG10.8403361344537815No Hit
GACGTCTAGCGTCGGTCCTTGTGTACTCGG10.8403361344537815No Hit
ACAAGCTGCCCGTCCCGTACTTTGTAACGGA10.8403361344537815No Hit
TGGCAGTTGGAAATGTAACGTCTGATTCGGAC10.8403361344537815No Hit
ACCAAGCCGCGCCTTACCACCCTAGCTACCT10.8403361344537815No Hit
CCGTCCCGTACTTTGTAA10.8403361344537815No Hit
TTGGCAGTTGGAAATGTAACGTCTGATTCGG10.8403361344537815No Hit
TACCAAGCCGCGCCTTACCACCCG10.8403361344537815No Hit
TCGGTCCTTGTGTACTCGGTTTATGAGCAC10.8403361344537815No Hit
GCCGCGCCTTACCACCCT10.8403361344537815No Hit
AGCCGCGCCTTACCACCC10.8403361344537815No Hit
TAGGCAGTTGGAAATGTAACGTCTGATTCGGA10.8403361344537815No Hit
TTGGCAGTTGGAAATGTAACGTCTGATTCGGAC10.8403361344537815No Hit
TTCAACAAGCTGCCCGTCCCGTACTTTGT10.8403361344537815No Hit
CCCGTCCCGTACTTTGTAAC10.8403361344537815No Hit
GGTGCGGGGTTCGAAC10.8403361344537815No Hit
TCAACAAGCTGCCCGTCCCGTACTTTGTAAC10.8403361344537815No Hit
TTCAACAAGCTGCCCGTCCCGTACTTTG10.8403361344537815No Hit
ACCAAGCCGCGCCTTACCACCCTAG10.8403361344537815No Hit
TTACAAGCTGCCCGTCCCGTACT10.8403361344537815No Hit
TATTACAAGCTGCCCGTCCCGTACTTTGT10.8403361344537815No Hit
AAGCCGCGCCTTACCACCCT10.8403361344537815No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)