FastQCFastQC Report
Mon 30 Nov 2020
S34.15-35nt.3449.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.3449.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length15-30
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAATGTTAAGGGGGGACGTCACATT410.526315789473683No Hit
AAGGGCAGTAATGTTAAGGGGGGACGTC37.894736842105263No Hit
AAGGGCAGTAATGTTAAGGGGGGACGTTC37.894736842105263No Hit
GTTAGAACAGGAGGACC37.894736842105263No Hit
ATAAAAACGACAGTCAAGGAGAGCACA37.894736842105263No Hit
ATGGCCTACCGGAAGAACTCC25.263157894736842No Hit
TTAGAACAGGAGGACC25.263157894736842No Hit
AATTAAGGAATATTTATTGGATGGCCT25.263157894736842No Hit
AAGGGCAGTAATGTTAAGGGGGGACGT25.263157894736842No Hit
ATTGGATGGCCTACCGGAAGAACTC25.263157894736842No Hit
CCACAAGGGGTGGAG25.263157894736842No Hit
TGGCCTACCGGAAGAACTCCT25.263157894736842No Hit
TATTTATTGGATGGCCTACCGGAAGAACTC12.631578947368421No Hit
GCCTACCGGAAGAACTCC12.631578947368421No Hit
GGATGGCCTACCGGAAGAACTCCTAAGG12.631578947368421No Hit
GTTCTAACCATAAAC12.631578947368421No Hit
CCGGAAGAACTCCTAAGGGCAGT12.631578947368421No Hit
AGAGAGAGAAACCTGGATATCCCGCCACCT12.631578947368421No Hit
ACCGGAAGAACTCCTAAGGGCAGTAAT12.631578947368421No Hit
ATAGCGATTATGATG12.631578947368421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)