FastQCFastQC Report
Mon 16 Nov 2020
S34.15-35nt.3452.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.3452.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887
Sequences flagged as poor quality0
Sequence length15-35
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCTCGGTACTGACTCGACAAGCGCC1357.154213036565977No Hit
TATTGTCGAATAACCCACCTCTTGTTGG1276.7302596714361425No Hit
TTGGGCTCGGTACTGACTCGACAAGCGCCT884.663487016428193No Hit
TCTATATTGTCGAATAACCCACCTCTTGG542.8616852146263914No Hit
TTAACGACCCGCTCTGTTCCATATCGATA462.437731849496555No Hit
TATTGTCGAATAACCCACCTCTTGG442.3317435082140965No Hit
TATACGTCGTGTACGACGCGAGACCGCGGA392.066772655007949No Hit
TAAAAGGACCGATTCGGGCAGTGAGTGTC371.9607843137254901No Hit
TTAACGACCCGCTCTGTTCCATATCGATAT321.695813460519343No Hit
TTTGTTAACTGGCCGACTTCCCCCGCCT301.5898251192368837No Hit
TGAATTGCCGTGGCTCGGAAGTTGGGCTC271.4308426073131957No Hit
TCGGTACTGACTCGACAAGCGCCTCGTAC271.4308426073131957No Hit
CACGTCGTGTACGACGCGAGACCGCGGAG261.377848436671966No Hit
TAACGACCCGCTCTGTTCCATATCGATAT261.377848436671966No Hit
TCGGTACTGACTCGACAAGCGCCTCGTACT261.377848436671966No Hit
TGGGCTCGGTACTGACTCGACAAGCGCCT241.2718600953895072No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGAC191.00688924218336No Hit
AAAAGTCACCGTCGTCCTCATGACTGCCT191.00688924218336No Hit
TAACGACCCGCTCTGTTCCATATCGATATT191.00688924218336No Hit
TATTGTCGAATAACCCACCTCTTGGT180.9538950715421303No Hit
TGGGCTCGGTACTGACTCGACAAGCGCC150.7949125596184419No Hit
TGAATTGCGGTGGCTCGGAAGTTGGGCTC140.7419183889772125No Hit
TAACACAAGCCCGATCGCTCCGATACATG140.7419183889772125No Hit
TGGGGATCCCGTAAGAAATACACGTCGTGT130.688924218335983No Hit
TTGGGGATCCCGTAAGAAATACACGTCGTG130.688924218335983No Hit
TTAGATGGACCCGGTATAGGATTTTCGCTC120.6359300476947536No Hit
TATATTGTCGAATAACCCACCTCTTGG110.5829358770535241No Hit
TCCCTCTTCGAACGTAACACAAGCCCGATC110.5829358770535241No Hit
TAACGACCCGCTCTGTTCCATATCGATA100.5299417064122947No Hit
TCCCAAACTTCGACTCGGACCAGTGCCGG100.5299417064122947No Hit
CATTGTCGAATAACCCACCTCTTGG90.47694753577106513No Hit
TGCCGAAGCCATAAAAGGACCGATTCGGGC90.47694753577106513No Hit
TTCGACTGTTAGGTGGGCAAAATTTGGGA90.47694753577106513No Hit
TCGTGTACGACGCGAGACCGCGGAGGCA80.4239533651298357No Hit
TTCTTGTTCGGAAAAGTCGCTGGCTTCGT80.4239533651298357No Hit
CATTGTCGAATAACCCACCTCTTGGT80.4239533651298357No Hit
TATAGACGTTAACGGCGCTGAGGGGTGT80.4239533651298357No Hit
TCTATATTGTCGAATAACCCACCTCTTGGT80.4239533651298357No Hit
TTAGATGGACCCGGAATAGGATTTTCGCTC70.37095919448860626No Hit
TCAAAACTCCCTCTTCGAACGTAACACAAGC70.37095919448860626No Hit
CACGTCGTGTACGACGCGAGACCGCGGAGG70.37095919448860626No Hit
TGAATTGCCGTGGCTCGGAAGTTGGGCTCG70.37095919448860626No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGCC70.37095919448860626No Hit
TGTCTTTTGTTAACTGGCCGACTTCCCCC70.37095919448860626No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGCC60.3179650238473768No Hit
TGACTGCCTCCTGCCACCAATCCTTCTTGT60.3179650238473768No Hit
TACTTGGATTTGTGGGATGATGGCATCGC60.3179650238473768No Hit
TACAATTTCTGCATCGTTATAGCACTGAT60.3179650238473768No Hit
TCCGATACATGGATTGAACTTCCTGAGTGT60.3179650238473768No Hit
TCATCTTCTTGTTCGGAAAAGTCGCTGGCT60.3179650238473768No Hit
TTCGAAGGGTTTCGAGAAATTGGGACACT60.3179650238473768No Hit
TTATTAATGAGACCGCGGAGGACTATCTC60.3179650238473768No Hit
TAGATGGACCCGGAATAGGATTTTCGCTC60.3179650238473768No Hit
TATTGTCGAATAACCCACCTCTTGGTG50.2649708532061473No Hit
TGAGGACCAGGGGCTGTGTGAGGGCCTAA50.2649708532061473No Hit
TTAACGACCCGCTCTGTTCCATATCGA50.2649708532061473No Hit
TTGGGCTCGGTACTGACTCGACAAGCG50.2649708532061473No Hit
TAAAAGGACCGATTCGGGCAGTGAGTGTCTT50.2649708532061473No Hit
CTTGGATCGGAAATGTTCATGGGGACGTT50.2649708532061473No Hit
TATTCACTGGCTTCCGCTTCAAAACTCCT50.2649708532061473No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGC50.2649708532061473No Hit
TTAACGACCCGCTCTGTTCCATATCGAT50.2649708532061473No Hit
TGGGGATCCCGTAAGAAATACACGTCGTG50.2649708532061473No Hit
TGCAATATAGACGTTAACGGCGCTGAGGGG50.2649708532061473No Hit
AACACAAGCCCGATCGCTCCGATACATGG50.2649708532061473No Hit
TCCTGAGTGTCCGGAAGATCCTTCTGACG50.2649708532061473No Hit
TTTGAACGTACATAACTTTTCTCCGACCCT50.2649708532061473No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGACT50.2649708532061473No Hit
TCGGATCCTATTGCATATGCGGATGCATC50.2649708532061473No Hit
TAGACAGAATAATAACAGGAGTACGGCCCAC50.2649708532061473No Hit
TCTTCTTGTTCGGAAAAGTCGCTGGCTTCG40.21197668256491786No Hit
TTGGATTTGTGGGATGATGGCATCGCCATTTT40.21197668256491786No Hit
TACACGTCGTGTACGACGCGAGACCGCGGA40.21197668256491786No Hit
TGGCTTCGTATTCACTGGCTTCCGCTTCA40.21197668256491786No Hit
TCGACTGTTAGGTGGGCAAAATTTGGGA40.21197668256491786No Hit
TATACGTCGTGTACGACGCGAGACCGCGGAT40.21197668256491786No Hit
TTGGGGATCCCGTAAGAAATACACGTCGT40.21197668256491786No Hit
ATTGTCGAATAACCCACCTCTTGTTGG40.21197668256491786No Hit
TGAGGACCAGGGGCTGTGTGAGGGCCTA40.21197668256491786No Hit
TACTGACTCGACAAGCGCCTCGTACTTGT40.21197668256491786No Hit
GGATGAAGACGCAGCGGCATGTTGAACTG40.21197668256491786No Hit
GATGTTGGGAAGGGGATACCTATCGCCT40.21197668256491786No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGC40.21197668256491786No Hit
CACAAGCCCGATCGCTCCGATACATGGATT40.21197668256491786No Hit
TCTACATTGTCGAATAACCCACCTCTTGGT40.21197668256491786No Hit
TAGGGAATTTTTGAATGGCTGCTATTTT40.21197668256491786No Hit
TTCTACTTGGATTTGTGGGATGATGGCATC40.21197668256491786No Hit
TCTGAGAAAAAGTCACCGTCGTCCTCATG40.21197668256491786No Hit
TGCAATATAGACGTTAACGGCGCTGAGGG30.1589825119236884No Hit
ACCGCGGAGGCACTCTTTGAAGCAGT30.1589825119236884No Hit
TTAGACAGAATAATAACAGGAGTACGGCCC30.1589825119236884No Hit
TGTCGATTAACGAGCTCCACACTTCTTTGG30.1589825119236884No Hit
TGTTAACTGGCCGACTTCCCCCGCCT30.1589825119236884No Hit
TTTTTTTGGGCGGATTCTTTCGGGGGT30.1589825119236884No Hit
TCGGTACTGACTCGACAAGCGCCTCGTA30.1589825119236884No Hit
TAGACGTTAACGGCGCTGAGGGGTGTTG30.1589825119236884No Hit
TCTTCTTGTTCGGAAAAGTCGCTGGCTTC30.1589825119236884No Hit
CTTCTTGTTCGGAAAAGTCGC30.1589825119236884No Hit
CGCGAGACCGCGGAGGCACTCTTTGAAGG30.1589825119236884No Hit
TGGCAGGAGATTGAACGTTTGTTTCGGTG30.1589825119236884No Hit
CACAGACGCGGTGTTTACTCTCTG30.1589825119236884No Hit
GACCGCGGAGGCACTCTTTGAAGG30.1589825119236884No Hit
TCAAAACTCCCTCTTCGAACGTAACACAAG30.1589825119236884No Hit
CACAAGCCCGATCGCTCCGATACATGGAT30.1589825119236884No Hit
TCACACACACTACTGATACTATCGGTCTCA30.1589825119236884No Hit
TATTGTCGAATAACCCACCTCTTGTTG30.1589825119236884No Hit
TTCGAAGGGTTTCGAGAAATTGGGACA30.1589825119236884No Hit
CCGTCGTCCTCATGACTGCCTCCTGCCACC30.1589825119236884No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGCCT30.1589825119236884No Hit
TGCCACGACGCATTTTCGTAGTTCTCCCCA30.1589825119236884No Hit
TGTACAATTTCTGCATCGTTATAGCACTGAT30.1589825119236884No Hit
ATTGTCGAATAACCCACCTCTTGG30.1589825119236884No Hit
TACTGACTCGACAAGCGCCTCGTACTTG30.1589825119236884No Hit
TTCGAGAAATTGGGACACTGTAATATTGGG30.1589825119236884No Hit
TGCAGTCGTGGATGACGTGGACGTGGTCGT30.1589825119236884No Hit
TTCCAACGTCTCGTTCCATCGGATCACTT30.1589825119236884No Hit
TCTTGGATCGGAAATGTTCATGGGGACGTT30.1589825119236884No Hit
TGGCCGACTTCCCCCGCCTGAAAGTGGTC30.1589825119236884No Hit
TCTTGTGAATTCGACTGTTAGGTGGGCA30.1589825119236884No Hit
TGTCTTTTGTTAACTGGCCGACTTCCCCT30.1589825119236884No Hit
TTGGATCGGAAATGTTCATGGGGACGTT30.1589825119236884No Hit
TCTGAGTATGGGGCTATTTCCGTGTGGAAA30.1589825119236884No Hit
TACAACTCTGCCATAGACACGTCCATTAGT30.1589825119236884No Hit
TCACAATTTCCAACGTCTCGTTCCATCGGA30.1589825119236884No Hit
TCAGTGTTGAAGGCTGTTTTTGGAATTGA30.1589825119236884No Hit
CACATGAGTTGAATTGCCGTGGCTCGGAA30.1589825119236884No Hit
TCGTGGATGACGTGGACGTGGTCGTTGTG30.1589825119236884No Hit
TTTTGTTAACTGGCCGACTTCCCCCGCCT30.1589825119236884No Hit
TGGATCGGAAATATTCATGGGGACGTTT30.1589825119236884No Hit
TCCTCATGACTGCCTCCTGCCACCAATCC30.1589825119236884No Hit
TGCATGAAGGAAGATTGATGGCAGGAGAT30.1589825119236884No Hit
TTGTACAATTTCTGCATCGTTATAGCACT30.1589825119236884No Hit
TACACGTCGTGTACGACGCGAGACCGCGGAGGCA30.1589825119236884No Hit
TGCCGAACTCTGAGAAAAAGTCACCGTCGTC30.1589825119236884No Hit
TGTCTTTTGTTAACTGGCCGACTTCCCCCG30.1589825119236884No Hit
CACGTCGTGTACGACGCGAGACCG30.1589825119236884No Hit
TGTGTCAGTGTTACTAATGATTCCTTCTTC30.1589825119236884No Hit
GACCGCGGAGGCACTCTTTGAAGCAGT20.10598834128245893No Hit
TCGATTAACGAACTCCACACTTCTTTGGG20.10598834128245893No Hit
TCTCGATTGATTAAAACTGCACAGACGCG20.10598834128245893No Hit
TCGTACTTGTCGATTAACGAGCTCCACACT20.10598834128245893No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGCCT20.10598834128245893No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGCCC20.10598834128245893No Hit
TTTACGGTAGTAGCCTAGTAATCCGAGAAA20.10598834128245893No Hit
TATCTCGATTGATTAAAACTGCACAGACGC20.10598834128245893No Hit
TCGTATTCACTGGCTTCCGCTTCAAAACT20.10598834128245893No Hit
TCACTGGCTTCCGCTTCAAAACTCCTCTCG20.10598834128245893No Hit
TTCGAGAAATTGGGACACTGTAATATTGG20.10598834128245893No Hit
TTTACGGTAGTAGCCTAGTAATCCGAGAA20.10598834128245893No Hit
TCTTGCTTTCGACGCGCTAGAGTGGCTGC20.10598834128245893No Hit
TGCGCCGGATGAAGACGCAGCGGCATGTTG20.10598834128245893No Hit
TACTGACTCGACAAGCGCCTCGTACT20.10598834128245893No Hit
TTGGGCGGATTCTTTCGGGGGTGACA20.10598834128245893No Hit
CAATATAGACGTTAACGGCGCTGAGGGG20.10598834128245893No Hit
TTCTTGTTCGGAAAAGTCGCTGGCTTCGTA20.10598834128245893No Hit
TCATGACTGCCTCCTGCCACCAATCCT20.10598834128245893No Hit
TGAACGTTTGTTTCGGTGTCCACACGAGAA20.10598834128245893No Hit
TAACAGGAGTACGGCCCACAGTAGCGTCGCCT20.10598834128245893No Hit
CTTGTTGTAGACCTCTGTCGCACGGCCC20.10598834128245893No Hit
TATAGACGTTAACGGCGCTGAGGGGTGTT20.10598834128245893No Hit
TTAACGACCCGCTCTGTTCCATATCGATATT20.10598834128245893No Hit
TGGGGATCCCGTAAGAAATACAC20.10598834128245893No Hit
CACGACGCATTTTCGTAGTTCTCCCCAAG20.10598834128245893No Hit
TAACAGGAGTACGGCCCACAGTAGCGTCGCT20.10598834128245893No Hit
TCTAACAAACTGACGGACACAAACCCTGAA20.10598834128245893No Hit
TAGACGTTAACGGCGCTGAGGGGTGT20.10598834128245893No Hit
TATTGTCGAATAACCCACCTCTTGTT20.10598834128245893No Hit
TATACGTCGTGTACGACGCGAGACCGCGGAC20.10598834128245893No Hit
TAACAGGAGTACGGCCCACAGTAGCGTCGCC20.10598834128245893No Hit
AACGACCCGCTCTGTTCCATATCGATATTGT20.10598834128245893No Hit
TCATGACTGCCTCCTGCCACCAATCCTTC20.10598834128245893No Hit
CTTGCGGCTCATTCCAAAGAAGAATTCTT20.10598834128245893No Hit
ACGTGGTCGTTGTGGAGGCACATGAGTTT20.10598834128245893No Hit
TCTACTTGGATTTGTGGGATGATGGCATC20.10598834128245893No Hit
TCCAGACTTCTTTGGGGATCCCGTAAGAAA20.10598834128245893No Hit
CATTGTCGAATAACCCACCTCTTG20.10598834128245893No Hit
TTGGGCGGATTCTTTCGGGGGTGACAATAT20.10598834128245893No Hit
TGCATGAAGGAAGATTGATGGCAGGAGATT20.10598834128245893No Hit
TGCATCGGTAGTTAATTCGAAGGGTTTCGA20.10598834128245893No Hit
TCAGTGATTTCTGAAGGGATGTGATGGGT20.10598834128245893No Hit
TTCGAACGTAACACAAGCCCGATCGCTCCGATA20.10598834128245893No Hit
TGCTTTCGACGCGCTAGAGTGGCTGCAT20.10598834128245893No Hit
TACATGGATTGAACTTCCTGAGTGTCCGGAA20.10598834128245893No Hit
TTAACGACCCGCTCTGTTCCATATCGATC20.10598834128245893No Hit
TTGAATTGCCGTGGCTCGGAAGTTGGGC20.10598834128245893No Hit
CTGGCCGACTTCCCCCGCCTGAAAGTGGTC20.10598834128245893No Hit
TCTGCCATAGACACGTCCATTAGTTCAAGC20.10598834128245893No Hit
ACGACCCGCTCTGTTCCATATCGATATTGTC20.10598834128245893No Hit
CACGTCGTGTACGACGCGAGACCGCGGA20.10598834128245893No Hit
TTCGGTAGGGTCTATGTGCGAGTCACGAA20.10598834128245893No Hit
TTATTAATGAGACCGCGGAGGACTATC20.10598834128245893No Hit
TCTTGTTCGGAAAAGTCGCTGGCTTCGTA20.10598834128245893No Hit
TCTGTTGTTGGGAATTCCATTTCTGAATTA20.10598834128245893No Hit
GATGCGAGACCGCGGAGGCACTCTTTGAAG20.10598834128245893No Hit
TCGGTAGGGTCTATGTGCGAGTCACGAAT20.10598834128245893No Hit
TCGAAGGGTTTCGAGAAATTGGGACACT20.10598834128245893No Hit
TAACACAAGCCCGATCGCTCCGATACATGG20.10598834128245893No Hit
TCCTTCTGACGAATTCGAATCTGCGTTTC20.10598834128245893No Hit
TAACAGGAGTACGGCCCACAGTAGCGTCGC20.10598834128245893No Hit
TGTTGGTTCTGCTTGCGGCTCATTCCAAA20.10598834128245893No Hit
TCGCAAGTGTTTAAGATGTTCTTCTAGGG20.10598834128245893No Hit
TTCACTGGCTTCCGCTTCAAAACTCCTCT20.10598834128245893No Hit
TCTGCATCGTTATAGCACTGATAGAATAGT20.10598834128245893No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGA20.10598834128245893No Hit
TTGAATTGCCGTGGCTCGGAAGTTGGGCTC20.10598834128245893No Hit
TGATTTCATCTGTGTTTGTGTTCGTGG20.10598834128245893No Hit
CCCTCTTCGAACGTAACACAAGCCCGATCGC20.10598834128245893No Hit
ATTTTCGTAGTTCTCCCCAAGTTTTTGTTT20.10598834128245893No Hit
TAACTGCAGTCGTGGATGACGTGGACGT20.10598834128245893No Hit
TCGTGGATGACGTGGACGTGGTCGTTGT20.10598834128245893No Hit
TCTGCATCGTTATAGCACTGATAGAATAG20.10598834128245893No Hit
TGGTGTAACTGCAGTCGTGGATGACGTGG20.10598834128245893No Hit
TAGGGAATTTTTGAATGGCTGCTATTT20.10598834128245893No Hit
TCGCCAGGTACGCCCTCCGATTTGAACGTAC20.10598834128245893No Hit
TGGGCTCGGTACTGACTCGACAAGCG20.10598834128245893No Hit
TAGATGGACCCGGTATAGGATTTTCGCTC20.10598834128245893No Hit
TATGCGCCGGATGAAGACGCAGCGGCATG20.10598834128245893No Hit
TCGTTCCATCGGATCACTTGGCGTTTGAGT20.10598834128245893No Hit
TCTACTTGGATTTGTGGGATGATGGCATCGC20.10598834128245893No Hit
TCGTACAACTCTGCCATAGACACGTCCATT20.10598834128245893No Hit
TATTAATGAGACCGCGGAGGACTATCTC20.10598834128245893No Hit
TAGAGTGGCTGCATGAAGGAAGGTT20.10598834128245893No Hit
TCAATCTTTTTGTGAATTCGACTGTTAGGT20.10598834128245893No Hit
TTCTAACAAACTGACGGACACAAACCCTG20.10598834128245893No Hit
TGTATCTTGGATCGGAAATGTTCATGGGG20.10598834128245893No Hit
TAACAGGAGTACGGCCCACAGTAGCGTC20.10598834128245893No Hit
CCCCCGCCTGAAAGTGGTCCATTAAGGCCT20.10598834128245893No Hit
TTTGTGAATTCGACTGTTAGGTGGGCAA20.10598834128245893No Hit
TCGGTACTGACTCGACAAGCGCCTCGTACTT20.10598834128245893No Hit
TGCCTCCTGCCACCAATCCTTCTTGTTT20.10598834128245893No Hit
TCTTCTTGTTCGGAAAAGTCGCTGGCTTCGT20.10598834128245893No Hit
CACGACGCATTTTCGTAGTTCTCCCCAAGC20.10598834128245893No Hit
TCCTCATGACTGCCTCCTGCCACCAATCCT20.10598834128245893No Hit
CTATATTGTCGAATAACCCACCTCTTGG20.10598834128245893No Hit
TAACGACCCGCTCTGTTCC20.10598834128245893No Hit
TCCTCTCGATTTGCGGGGAGGAGGACT20.10598834128245893No Hit
CCGCGGAGGCACTCTTTGAAGCAGTGCCA20.10598834128245893No Hit
TGGACGTGGTCGTTGTGGAGGCACATGAGT20.10598834128245893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)