FastQCFastQC Report
Mon 30 Nov 2020
S34.15-35nt.4554.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.4554.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747
Sequences flagged as poor quality0
Sequence length15-35
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCTCGGTACTGACTCGACAAGCGCC1357.7275329135661135No Hit
TATTGTCGAATAACCCACCTCTTGTTGG1277.269605037206641No Hit
TTGGGCTCGGTACTGACTCGACAAGCGCCT885.037206639954207No Hit
TCTATATTGTCGAATAACCCACCTCTTGG543.091013165426445No Hit
TTAACGACCCGCTCTGTTCCATATCGATA462.633085289066972No Hit
TATTGTCGAATAACCCACCTCTTGG442.5186033199771036No Hit
TATACGTCGTGTACGACGCGAGACCGCGGA392.2323983972524326No Hit
TAAAAGGACCGATTCGGGCAGTGAGTGTC372.117916428162564No Hit
TTAACGACCCGCTCTGTTCCATATCGATAT321.8317115054378934No Hit
TTTGTTAACTGGCCGACTTCCCCCGCCT301.7172295363480254No Hit
TGAATTGCCGTGGCTCGGAAGTTGGGCTC271.5455065827132226No Hit
TCGGTACTGACTCGACAAGCGCCTCGTAC271.5455065827132226No Hit
CACGTCGTGTACGACGCGAGACCGCGGAG261.4882655981682884No Hit
TAACGACCCGCTCTGTTCCATATCGATAT261.4882655981682884No Hit
TCGGTACTGACTCGACAAGCGCCTCGTACT261.4882655981682884No Hit
TGGGCTCGGTACTGACTCGACAAGCGCCT241.3737836290784202No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGAC191.0875787063537492No Hit
AAAAGTCACCGTCGTCCTCATGACTGCCT191.0875787063537492No Hit
TAACGACCCGCTCTGTTCCATATCGATATT191.0875787063537492No Hit
TATTGTCGAATAACCCACCTCTTGGT181.030337721808815No Hit
TGGGCTCGGTACTGACTCGACAAGCGCC150.8586147681740127No Hit
TGAATTGCGGTGGCTCGGAAGTTGGGCTC140.8013737836290785No Hit
TAACACAAGCCCGATCGCTCCGATACATG140.8013737836290785No Hit
TGGGGATCCCGTAAGAAATACACGTCGTGT130.7441327990841442No Hit
TTGGGGATCCCGTAAGAAATACACGTCGTG130.7441327990841442No Hit
TATATTGTCGAATAACCCACCTCTTGG110.6296508299942759No Hit
TCCCTCTTCGAACGTAACACAAGCCCGATC110.6296508299942759No Hit
TAACGACCCGCTCTGTTCCATATCGATA100.5724098454493417No Hit
TCCCAAACTTCGACTCGGACCAGTGCCGG100.5724098454493417No Hit
CATTGTCGAATAACCCACCTCTTGG90.5151688609044075No Hit
TGCCGAAGCCATAAAAGGACCGATTCGGGC90.5151688609044075No Hit
TTCGACTGTTAGGTGGGCAAAATTTGGGA90.5151688609044075No Hit
TCGTGTACGACGCGAGACCGCGGAGGCA80.45792787635947335No Hit
CATTGTCGAATAACCCACCTCTTGGT80.45792787635947335No Hit
TATAGACGTTAACGGCGCTGAGGGGTGT80.45792787635947335No Hit
TCTATATTGTCGAATAACCCACCTCTTGGT80.45792787635947335No Hit
TCAAAACTCCCTCTTCGAACGTAACACAAGC70.40068689181453926No Hit
CACGTCGTGTACGACGCGAGACCGCGGAGG70.40068689181453926No Hit
TGAATTGCCGTGGCTCGGAAGTTGGGCTCG70.40068689181453926No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGCC70.40068689181453926No Hit
TGTCTTTTGTTAACTGGCCGACTTCCCCC70.40068689181453926No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGCC60.34344590726960506No Hit
TGACTGCCTCCTGCCACCAATCCTTCTTGT60.34344590726960506No Hit
TACAATTTCTGCATCGTTATAGCACTGAT60.34344590726960506No Hit
TCCGATACATGGATTGAACTTCCTGAGTGT60.34344590726960506No Hit
TTCGAAGGGTTTCGAGAAATTGGGACACT60.34344590726960506No Hit
TTATTAATGAGACCGCGGAGGACTATCTC60.34344590726960506No Hit
TATTGTCGAATAACCCACCTCTTGGTG50.28620492272467085No Hit
TGAGGACCAGGGGCTGTGTGAGGGCCTAA50.28620492272467085No Hit
TTAACGACCCGCTCTGTTCCATATCGA50.28620492272467085No Hit
TTGGGCTCGGTACTGACTCGACAAGCG50.28620492272467085No Hit
TAAAAGGACCGATTCGGGCAGTGAGTGTCTT50.28620492272467085No Hit
CTTGGATCGGAAATGTTCATGGGGACGTT50.28620492272467085No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGC50.28620492272467085No Hit
TTAACGACCCGCTCTGTTCCATATCGAT50.28620492272467085No Hit
TGGGGATCCCGTAAGAAATACACGTCGTG50.28620492272467085No Hit
TGCAATATAGACGTTAACGGCGCTGAGGGG50.28620492272467085No Hit
AACACAAGCCCGATCGCTCCGATACATGG50.28620492272467085No Hit
TCCTGAGTGTCCGGAAGATCCTTCTGACG50.28620492272467085No Hit
TTTGAACGTACATAACTTTTCTCCGACCCT50.28620492272467085No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGACT50.28620492272467085No Hit
TCGGATCCTATTGCATATGCGGATGCATC50.28620492272467085No Hit
TAGACAGAATAATAACAGGAGTACGGCCCAC50.28620492272467085No Hit
TACACGTCGTGTACGACGCGAGACCGCGGA40.22896393817973668No Hit
TCGACTGTTAGGTGGGCAAAATTTGGGA40.22896393817973668No Hit
TATACGTCGTGTACGACGCGAGACCGCGGAT40.22896393817973668No Hit
TTGGGGATCCCGTAAGAAATACACGTCGT40.22896393817973668No Hit
ATTGTCGAATAACCCACCTCTTGTTGG40.22896393817973668No Hit
TGAGGACCAGGGGCTGTGTGAGGGCCTA40.22896393817973668No Hit
TACTGACTCGACAAGCGCCTCGTACTTGT40.22896393817973668No Hit
GGATGAAGACGCAGCGGCATGTTGAACTG40.22896393817973668No Hit
GATGTTGGGAAGGGGATACCTATCGCCT40.22896393817973668No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGC40.22896393817973668No Hit
CACAAGCCCGATCGCTCCGATACATGGATT40.22896393817973668No Hit
TCTACATTGTCGAATAACCCACCTCTTGGT40.22896393817973668No Hit
TAGGGAATTTTTGAATGGCTGCTATTTT40.22896393817973668No Hit
TCTGAGAAAAAGTCACCGTCGTCCTCATG40.22896393817973668No Hit
TGCAATATAGACGTTAACGGCGCTGAGGG30.17172295363480253No Hit
ACCGCGGAGGCACTCTTTGAAGCAGT30.17172295363480253No Hit
TTAGACAGAATAATAACAGGAGTACGGCCC30.17172295363480253No Hit
TGTCGATTAACGAGCTCCACACTTCTTTGG30.17172295363480253No Hit
TGTTAACTGGCCGACTTCCCCCGCCT30.17172295363480253No Hit
TTTTTTTGGGCGGATTCTTTCGGGGGT30.17172295363480253No Hit
TCGGTACTGACTCGACAAGCGCCTCGTA30.17172295363480253No Hit
TAGACGTTAACGGCGCTGAGGGGTGTTG30.17172295363480253No Hit
CGCGAGACCGCGGAGGCACTCTTTGAAGG30.17172295363480253No Hit
TGGCAGGAGATTGAACGTTTGTTTCGGTG30.17172295363480253No Hit
GACCGCGGAGGCACTCTTTGAAGG30.17172295363480253No Hit
TCAAAACTCCCTCTTCGAACGTAACACAAG30.17172295363480253No Hit
CACAAGCCCGATCGCTCCGATACATGGAT30.17172295363480253No Hit
TATTGTCGAATAACCCACCTCTTGTTG30.17172295363480253No Hit
TTCGAAGGGTTTCGAGAAATTGGGACA30.17172295363480253No Hit
CCGTCGTCCTCATGACTGCCTCCTGCCACC30.17172295363480253No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGCCT30.17172295363480253No Hit
TGCCACGACGCATTTTCGTAGTTCTCCCCA30.17172295363480253No Hit
TGTACAATTTCTGCATCGTTATAGCACTGAT30.17172295363480253No Hit
ATTGTCGAATAACCCACCTCTTGG30.17172295363480253No Hit
TACTGACTCGACAAGCGCCTCGTACTTG30.17172295363480253No Hit
TTCGAGAAATTGGGACACTGTAATATTGGG30.17172295363480253No Hit
TGCAGTCGTGGATGACGTGGACGTGGTCGT30.17172295363480253No Hit
TTCCAACGTCTCGTTCCATCGGATCACTT30.17172295363480253No Hit
TCTTGGATCGGAAATGTTCATGGGGACGTT30.17172295363480253No Hit
TGGCCGACTTCCCCCGCCTGAAAGTGGTC30.17172295363480253No Hit
TCTTGTGAATTCGACTGTTAGGTGGGCA30.17172295363480253No Hit
TGTCTTTTGTTAACTGGCCGACTTCCCCT30.17172295363480253No Hit
TTGGATCGGAAATGTTCATGGGGACGTT30.17172295363480253No Hit
TACAACTCTGCCATAGACACGTCCATTAGT30.17172295363480253No Hit
TCACAATTTCCAACGTCTCGTTCCATCGGA30.17172295363480253No Hit
TCAGTGTTGAAGGCTGTTTTTGGAATTGA30.17172295363480253No Hit
CACATGAGTTGAATTGCCGTGGCTCGGAA30.17172295363480253No Hit
TCGTGGATGACGTGGACGTGGTCGTTGTG30.17172295363480253No Hit
TTTTGTTAACTGGCCGACTTCCCCCGCCT30.17172295363480253No Hit
TGGATCGGAAATATTCATGGGGACGTTT30.17172295363480253No Hit
TCCTCATGACTGCCTCCTGCCACCAATCC30.17172295363480253No Hit
TGCATGAAGGAAGATTGATGGCAGGAGAT30.17172295363480253No Hit
TTGTACAATTTCTGCATCGTTATAGCACT30.17172295363480253No Hit
TACACGTCGTGTACGACGCGAGACCGCGGAGGCA30.17172295363480253No Hit
TGCCGAACTCTGAGAAAAAGTCACCGTCGTC30.17172295363480253No Hit
TGTCTTTTGTTAACTGGCCGACTTCCCCCG30.17172295363480253No Hit
CACGTCGTGTACGACGCGAGACCG30.17172295363480253No Hit
TGTGTCAGTGTTACTAATGATTCCTTCTTC30.17172295363480253No Hit
GACCGCGGAGGCACTCTTTGAAGCAGT20.11448196908986834No Hit
TTGTTCACTTTCATCTTCTCCTCTCGAT20.11448196908986834No Hit
TCGATTAACGAACTCCACACTTCTTTGGG20.11448196908986834No Hit
TCGTACTTGTCGATTAACGAGCTCCACACT20.11448196908986834No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGCCT20.11448196908986834No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGCCC20.11448196908986834No Hit
TTTACGGTAGTAGCCTAGTAATCCGAGAAA20.11448196908986834No Hit
TTCGAGAAATTGGGACACTGTAATATTGG20.11448196908986834No Hit
TTTACGGTAGTAGCCTAGTAATCCGAGAA20.11448196908986834No Hit
TCTTGCTTTCGACGCGCTAGAGTGGCTGC20.11448196908986834No Hit
TGCGCCGGATGAAGACGCAGCGGCATGTTG20.11448196908986834No Hit
TACTGACTCGACAAGCGCCTCGTACT20.11448196908986834No Hit
TTGGGCGGATTCTTTCGGGGGTGACA20.11448196908986834No Hit
CAATATAGACGTTAACGGCGCTGAGGGG20.11448196908986834No Hit
TCATGACTGCCTCCTGCCACCAATCCT20.11448196908986834No Hit
TGAACGTTTGTTTCGGTGTCCACACGAGAA20.11448196908986834No Hit
TAACAGGAGTACGGCCCACAGTAGCGTCGCCT20.11448196908986834No Hit
CTTGTTGTAGACCTCTGTCGCACGGCCC20.11448196908986834No Hit
TATAGACGTTAACGGCGCTGAGGGGTGTT20.11448196908986834No Hit
TTAACGACCCGCTCTGTTCCATATCGATATT20.11448196908986834No Hit
TGGGGATCCCGTAAGAAATACAC20.11448196908986834No Hit
CACGACGCATTTTCGTAGTTCTCCCCAAG20.11448196908986834No Hit
TAACAGGAGTACGGCCCACAGTAGCGTCGCT20.11448196908986834No Hit
TAGACGTTAACGGCGCTGAGGGGTGT20.11448196908986834No Hit
TATTGTCGAATAACCCACCTCTTGTT20.11448196908986834No Hit
TATACGTCGTGTACGACGCGAGACCGCGGAC20.11448196908986834No Hit
TAACAGGAGTACGGCCCACAGTAGCGTCGCC20.11448196908986834No Hit
AACGACCCGCTCTGTTCCATATCGATATTGT20.11448196908986834No Hit
TCATGACTGCCTCCTGCCACCAATCCTTC20.11448196908986834No Hit
CTTGCGGCTCATTCCAAAGAAGAATTCTT20.11448196908986834No Hit
ACGTGGTCGTTGTGGAGGCACATGAGTTT20.11448196908986834No Hit
TCCAGACTTCTTTGGGGATCCCGTAAGAAA20.11448196908986834No Hit
CATTGTCGAATAACCCACCTCTTG20.11448196908986834No Hit
TTGGGCGGATTCTTTCGGGGGTGACAATAT20.11448196908986834No Hit
TGCATGAAGGAAGATTGATGGCAGGAGATT20.11448196908986834No Hit
TGCATCGGTAGTTAATTCGAAGGGTTTCGA20.11448196908986834No Hit
TTCGAACGTAACACAAGCCCGATCGCTCCGATA20.11448196908986834No Hit
TGCTTTCGACGCGCTAGAGTGGCTGCAT20.11448196908986834No Hit
TACATGGATTGAACTTCCTGAGTGTCCGGAA20.11448196908986834No Hit
TTAACGACCCGCTCTGTTCCATATCGATC20.11448196908986834No Hit
TTGAATTGCCGTGGCTCGGAAGTTGGGC20.11448196908986834No Hit
CTGGCCGACTTCCCCCGCCTGAAAGTGGTC20.11448196908986834No Hit
TCTGCCATAGACACGTCCATTAGTTCAAGC20.11448196908986834No Hit
ACGACCCGCTCTGTTCCATATCGATATTGTC20.11448196908986834No Hit
CACGTCGTGTACGACGCGAGACCGCGGA20.11448196908986834No Hit
TTATTAATGAGACCGCGGAGGACTATC20.11448196908986834No Hit
GATGCGAGACCGCGGAGGCACTCTTTGAAG20.11448196908986834No Hit
TCGAAGGGTTTCGAGAAATTGGGACACT20.11448196908986834No Hit
TAACACAAGCCCGATCGCTCCGATACATGG20.11448196908986834No Hit
TCCTTCTGACGAATTCGAATCTGCGTTTC20.11448196908986834No Hit
TAACAGGAGTACGGCCCACAGTAGCGTCGC20.11448196908986834No Hit
TGTTGGTTCTGCTTGCGGCTCATTCCAAA20.11448196908986834No Hit
TCGCAAGTGTTTAAGATGTTCTTCTAGGG20.11448196908986834No Hit
TCTGCATCGTTATAGCACTGATAGAATAGT20.11448196908986834No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGA20.11448196908986834No Hit
TTGAATTGCCGTGGCTCGGAAGTTGGGCTC20.11448196908986834No Hit
TGATTTCATCTGTGTTTGTGTTCGTGG20.11448196908986834No Hit
CCCTCTTCGAACGTAACACAAGCCCGATCGC20.11448196908986834No Hit
ATTTTCGTAGTTCTCCCCAAGTTTTTGTTT20.11448196908986834No Hit
TAACTGCAGTCGTGGATGACGTGGACGT20.11448196908986834No Hit
TCGTGGATGACGTGGACGTGGTCGTTGT20.11448196908986834No Hit
TTGTTCACTTTCATCTTCTCCTCTCGATTT20.11448196908986834No Hit
TCTGCATCGTTATAGCACTGATAGAATAG20.11448196908986834No Hit
TGGTGTAACTGCAGTCGTGGATGACGTGG20.11448196908986834No Hit
TAGGGAATTTTTGAATGGCTGCTATTT20.11448196908986834No Hit
TCGCCAGGTACGCCCTCCGATTTGAACGTAC20.11448196908986834No Hit
TGGGCTCGGTACTGACTCGACAAGCG20.11448196908986834No Hit
TATGCGCCGGATGAAGACGCAGCGGCATG20.11448196908986834No Hit
TCGTTCCATCGGATCACTTGGCGTTTGAGT20.11448196908986834No Hit
TCGTACAACTCTGCCATAGACACGTCCATT20.11448196908986834No Hit
TATTAATGAGACCGCGGAGGACTATCTC20.11448196908986834No Hit
TAGAGTGGCTGCATGAAGGAAGGTT20.11448196908986834No Hit
TCAATCTTTTTGTGAATTCGACTGTTAGGT20.11448196908986834No Hit
TGTATCTTGGATCGGAAATGTTCATGGGG20.11448196908986834No Hit
TAACAGGAGTACGGCCCACAGTAGCGTC20.11448196908986834No Hit
CCCCCGCCTGAAAGTGGTCCATTAAGGCCT20.11448196908986834No Hit
TTTGTGAATTCGACTGTTAGGTGGGCAA20.11448196908986834No Hit
TCGGTACTGACTCGACAAGCGCCTCGTACTT20.11448196908986834No Hit
TGCCTCCTGCCACCAATCCTTCTTGTTT20.11448196908986834No Hit
CACGACGCATTTTCGTAGTTCTCCCCAAGC20.11448196908986834No Hit
TCCTCATGACTGCCTCCTGCCACCAATCCT20.11448196908986834No Hit
CTATATTGTCGAATAACCCACCTCTTGG20.11448196908986834No Hit
TAACGACCCGCTCTGTTCC20.11448196908986834No Hit
TCCTCTCGATTTGCGGGGAGGAGGACT20.11448196908986834No Hit
CCGCGGAGGCACTCTTTGAAGCAGTGCCA20.11448196908986834No Hit
TGGACGTGGTCGTTGTGGAGGCACATGAGT20.11448196908986834No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)