FastQCFastQC Report
Mon 30 Nov 2020
S34.raw.12682.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.12682.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225
Sequences flagged as poor quality0
Sequence length11-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCCCCGAAAACAGCCAGTCACAGGATAG177.555555555555555No Hit
TAGGTGGGCCCCGAAAACAGCCAGTCGCAG73.111111111111111No Hit
TACATGCGAAAAATCTTCGACCCCGCCGG62.666666666666667No Hit
TAGGTGGGCCCCGAAAACAGCCAGTCACA52.2222222222222223No Hit
GGCCCCGCCGGGAATCGAACTC52.2222222222222223No Hit
TGCGAAAAATCTTCGACCCCGCCGGGAAT41.7777777777777777No Hit
TTTTACCCACAATGCTGACACCGAGTCGCT41.7777777777777777No Hit
TAGGTGGGCCCCGAAAACAGCCAGTCACAAA41.7777777777777777No Hit
TTTTACCTACAATGCTGACACCGAGTCGCT41.7777777777777777No Hit
CGTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCAC41.7777777777777777No Hit
TGCAGTGATCATCGCGCCATGGGAGCTG31.3333333333333335No Hit
ACAGGATAGAACATTTTACATGCGAAAAATCTTCGCCCCCGCCGG31.3333333333333335No Hit
GAAAACAGCCAGTCACAGGATAGAACATTTTACATGC31.3333333333333335No Hit
AGAACATTTTACATGCGAAAAATCTTCGCCCCGCCGGGAA31.3333333333333335No Hit
CCGCCCCCGCCGGGAATCGAAC31.3333333333333335No Hit
TCGCACCCGTCGGGAATCGAACTCGGGACTCCCGCGTGCAAGGCGAATGCAGTGATCATTGCGCCACG31.3333333333333335No Hit
TAGGTGGGCCCCGAAAACAGCCAGTCACAG31.3333333333333335No Hit
TTTTCCTCACGAGGCTAGGTGGACCC31.3333333333333335No Hit
CACGAGGCTAGGTGGGCCCCG31.3333333333333335No Hit
CCCACAATGCTGACACCGAGTCGCTATGCGCATT31.3333333333333335No Hit
TTTTACCCACAATGCTGACACCGAGTCGCTA31.3333333333333335No Hit
GGCTAGGTGGGCCCCGA31.3333333333333335No Hit
CATTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCG31.3333333333333335No Hit
AATCGAACTCGGGACTCCCGCGTGCAAGGCGAATGCAGT31.3333333333333335No Hit
TTTTACCTACAATGCTGACACCGAGTCGCTATGCGCATT20.8888888888888888No Hit
CTAGGTGGGCCCCGA20.8888888888888888No Hit
TCCTCACGAGACTAGGTGGGCCCCGAAA20.8888888888888888No Hit
TTACCTACAATGCTGACACCGAGTCGCTAT20.8888888888888888No Hit
TCACGAGGATAGGTGGGCCCCGAAAACAGC20.8888888888888888No Hit
TCCTCACGAGGCTAGGTGGGCCCCGAAA20.8888888888888888No Hit
CTATTTTCCTCACGAGGCTAGGTGT20.8888888888888888No Hit
TACCTATTTTCCTCACGAGGCTAGGTGGGC20.8888888888888888No Hit
TTTTACATGCGAAAAATCTTCGCCCCCGCC20.8888888888888888No Hit
TTACCCACAAAGCTGACACCGAGTCGCTATGCGCAT20.8888888888888888No Hit
TATTTTCCTCACGAGGCTAGGTGGGCCCTG20.8888888888888888No Hit
TATTTTCCTCACGAGGCTAGGTGGGCCC20.8888888888888888No Hit
TTTTCTTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAG20.8888888888888888No Hit
GCCGGGAATCGAACTC20.8888888888888888No Hit
TAATTAAATTTTACCCACAAAGCTGACACC20.8888888888888888No Hit
CCAAGACTCCCGCGTGCAAGGCGAAT20.8888888888888888No Hit
TTTTACATGCGAAAAATCTTCGACCCCGCC20.8888888888888888No Hit
TTCCTCACGAGGCTAGGTGGGCCCCGAAA20.8888888888888888No Hit
TAAATTTTACCCACAATGCTGACACCGAGT20.8888888888888888No Hit
TATTTTCCTCACAAGGCTAGGTGGGCCCC20.8888888888888888No Hit
GAAAACAGCCAGTCACAGGATAGAACATTTTACATGCGAAAAATCTTCGCCCCCGCCGGGAATCGAACCCGGGAC10.4444444444444444No Hit
TATTCGCCCCCGCCGG10.4444444444444444No Hit
TACCCACAATGCTGACACCGAGTCGCTATGCGCA10.4444444444444444No Hit
AGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAACATTT10.4444444444444444No Hit
TTTTACCCACAATGCTGACACCGAGTCGCTATGCGC10.4444444444444444No Hit
TTATTTCATAGCTACCTATTTTCCTCACGAG10.4444444444444444No Hit
TAGGTGGGCCCCGAAAACAGCCAGTCACAGGAT10.4444444444444444No Hit
TAGGTGGGCCCCGAAAACAGCCAGTCACAGGA10.4444444444444444No Hit
TCTTCGCCCCCGCCGGGAATCGAACCCGAG10.4444444444444444No Hit
TAATTAAATTTTACCCACAATGCTGACACCGAGTCGCTATGCGC10.4444444444444444No Hit
ATCCAAGATAATTAAATTTTACCCACAATGCTGACACCGAGTCGCTATGCGCATATTATTACA10.4444444444444444No Hit
CTACAGTGAAG10.4444444444444444No Hit
CCCGTGGTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAACATTTTACATGC10.4444444444444444No Hit
CCCCGCCGGGAATCGAACTCGGGATCTC10.4444444444444444No Hit
AGATAATTAAATTTTACCTACAATGCTGACACCTAGTCGCTATGCCCATT10.4444444444444444No Hit
TCAGGTTGTAGT10.4444444444444444No Hit
TGCAGTGATCATTGCGCCATGGGAGCTG10.4444444444444444No Hit
TTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCACTCACA10.4444444444444444No Hit
AAACAGCCAGTCACAGGATAGAACATTTTACATGCGAAAAATCTTCGCACCCGCCGGGAATCGAACC10.4444444444444444No Hit
TGCAGTGACCATTGCGCCATGGGAGCTGA10.4444444444444444No Hit
CGGTGTCAACTG10.4444444444444444No Hit
TCTCCCGCGTGCAAGGCG10.4444444444444444No Hit
TTACCCACAATGCTGACACCGAGTCGCTAT10.4444444444444444No Hit
GTCGCCCCCGCC10.4444444444444444No Hit
TGCAGTGATCATCGCGCCATGGGAGCTGA10.4444444444444444No Hit
CGAATGCAGTGACCATTGCGCCATGGGAGC10.4444444444444444No Hit
ATTTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGATAAA10.4444444444444444No Hit
AATCCAAGATAATTAAATTTTACCCACAAAGCTGACACC10.4444444444444444No Hit
ACAGCCAGTCACAGGATAGAACATTTTACAT10.4444444444444444No Hit
TAGGTGGGCCCCGAAAACAGCCAGTCACAA10.4444444444444444No Hit
CTAATGCGCATTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGT10.4444444444444444No Hit
TTCCTCACGAGGCTAGGTGGGTCCCGAAA10.4444444444444444No Hit
GTTGATCACCA10.4444444444444444No Hit
TAGGTGGGCCCCGAAAACTGCCAGTCACAA10.4444444444444444No Hit
TGATTATGGATACAGAGTGTACGACAAGGC10.4444444444444444No Hit
TTATTTCATAGCTACCTATTTTCCTCACGA10.4444444444444444No Hit
TAGGTGGGCCCCGAAAACAGCCAGTCACAGG10.4444444444444444No Hit
AATGCAGTGATCATTGCGCCATGGGAGC10.4444444444444444No Hit
TCCTCACGAGGTTAGGTGGGCCCCGAA10.4444444444444444No Hit
CTGCCGGTGTCA10.4444444444444444No Hit
TTTACACACAATGCTGACACCGAGTCGCTATGCGC10.4444444444444444No Hit
ATGCGCATTTTATTTCAGAGCTACCTATTTTCCTCACGAGGCTAGGTGGTCCACG10.4444444444444444No Hit
TGCAGTGATCATCGCGCCATGGGAGC10.4444444444444444No Hit
CAATGCTGACACCGAGTCGCTATGCGCAT10.4444444444444444No Hit
TTTCCTCACGAGGCTGGGTGGGCCCCGAAAACAGCC10.4444444444444444No Hit
TTTTACCCACAATGCTGACACCGAG10.4444444444444444No Hit
TAGGTGGGCCCCGAAAACAGCCAGTCGCA10.4444444444444444No Hit
AACAGCCAGTCACAGGATAGAACATTTTACATGCGAAAAATCTTCGCACCCGCCGGGAAT10.4444444444444444No Hit
TCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCAC10.4444444444444444No Hit
AGAACATTTTACATGCGAAAAATCTTCGCCCCCGCCGGGAA10.4444444444444444No Hit
GGCCCCGAAAACAGCCAGTCACAGGATAGAACATTTTAC10.4444444444444444No Hit
CCGCGTGCAAGGCGAATGCAGTGACC10.4444444444444444No Hit
ATGCGCATTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGG10.4444444444444444No Hit
TTTTACCCACAATGCTGACACCGAGTCGCTATGCGCATT10.4444444444444444No Hit
TAATTCAATTTTACCCACAATGCTGACACCAAGTCGCTATGCGCATTTTA10.4444444444444444No Hit
TTAAATTTTACCCACAATGCTGACACCAAAT10.4444444444444444No Hit
TCTCGGTTGCTAGAGGATTTTTATGCACT10.4444444444444444No Hit
TAATGCGCATTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTG10.4444444444444444No Hit
CGAAAACAGCCAGTCACAGGATAGAACATTTTACATGCGAA10.4444444444444444No Hit
TTTTACCTACAATGCTGACACCGAGTCGCTATGCGC10.4444444444444444No Hit
CCTACAATGCTGACACCGAGT10.4444444444444444No Hit
ATGCAGTGATCATCGCGCCATGGGAGCTGA10.4444444444444444No Hit
GACTCCCGCGTGCAAGGCGAATGCAGT10.4444444444444444No Hit
AATCTTCGACCCCGCCGGGAA10.4444444444444444No Hit
TATGCGCATTTTATTTCATAGCTACCTATT10.4444444444444444No Hit
TGCAAAAAATCTTCGCCCCCGCCGGGAA10.4444444444444444No Hit
GGCTAGGTGGGCCCCGAAAACAGCCAGTCACCGGATAGAACATTTTACATGCG10.4444444444444444No Hit
TAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGA10.4444444444444444No Hit
TTTACCCACAATGCTGACACCGAGTCGCTAT10.4444444444444444No Hit
TAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACG10.4444444444444444No Hit
CTATGCGCATTTTATTTCATAGCTACCTATTTTCCTC10.4444444444444444No Hit
TAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAACATTTTA10.4444444444444444No Hit
TTTTCCTCACGAGGATAGGTGGGCCCCGA10.4444444444444444No Hit
TTATTTTCCTCACGAGGCTAGGTGGGCC10.4444444444444444No Hit
ATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGAAAACA10.4444444444444444No Hit
TAAATTTTACCTACAATGCTGACACCGAG10.4444444444444444No Hit
AAAATCTTCGCCCCCGCCGGGAATCGAACCCGGGACTCCCGCGTGCAAGG10.4444444444444444No Hit
TACCCACAAAGCTGACACCGAGTCGCTATG10.4444444444444444No Hit
TTAAATTTTACCCACAATGCTGACACCGAG10.4444444444444444No Hit
TTCCTCACGAGGCTAGGT10.4444444444444444No Hit
TGCGAAAAATCTTCGACCCCGCCGGGAATC10.4444444444444444No Hit
GAAAACAGCCAGTCACAGGATAGAACATTTTACATGCGAATAATCTTCGCCCCCGCCGGGAATCGAACC10.4444444444444444No Hit
CATGTGAAAAATCTTCGCCCCCGCCGGGAATC10.4444444444444444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)