FastQCFastQC Report
Mon 30 Nov 2020
S34.raw.16656.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.16656.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2228
Sequences flagged as poor quality0
Sequence length11-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTCAGACTAGAGACAACCCGAGAGC63728.590664272890486No Hit
TTCCTGTCAGACTAGAGACAACCCGAGAGC2029.066427289048475No Hit
TTTTCCTGTCAGACTAGAGACAACCCGAG954.26391382405745No Hit
TCAGTTAGTACACCACACCGGGAGACGAC532.378815080789946No Hit
TCCTGTCAGACTAGAGACAACCCGAGAG522.333931777378815No Hit
TAGGTTCTTGAGAATATCGGCACGGAGC512.289048473967684No Hit
TCAGACTAGAGACAACCCGAGAGCTCAAT482.154398563734291No Hit
TTTTCCTGTCAGACTAGAGACAACCCGAGA431.9299820466786355No Hit
TTGGAGGAAAACTACGGCTCAACCCGGGC371.660682226211849No Hit
TTTCCTGTCAGACTAGAGACAACCCGAGA341.526032315978456No Hit
TTCCTGTCAGACTAGAGACAACCCGAGAG301.3464991023339317No Hit
TTTCCTGTCAGACTAGAGACAACCCGAGAG281.2567324955116697No Hit
TCAGACTAGAGACAACCCGAGAGCTCAATA251.1220825852782765No Hit
TCAGACTAGAGACAACCCGAGAGCTCAATAT220.9874326750448833No Hit
TTTCCTGTCAGACTAGAGACAACCCGAGAGC190.8527827648114902No Hit
TTCTTGAGAATATCGGCACGGAGCTCGC160.718132854578097No Hit
TTTGGAGGAAAACTACGGCTCAACCCGGGC160.718132854578097No Hit
TTCGTAATCCGCCTCACCTGTCCCTAACT160.718132854578097No Hit
TAGGTTCTTGAGAAAATCGGCACGGAGC140.6283662477558348No Hit
TTTTCCTGTCAGACTAGAGACAACCCGAGAG130.5834829443447037No Hit
TGCCCATGTTGCCTCCCGATTAGTACTCGAA130.5834829443447037No Hit
TCCCCTGGCTGTACCAATTGCCTGACAAAT110.49371633752244165No Hit
CTTTTCCTGTCAGACTAGAGACAACCCGAG100.4488330341113106No Hit
TCCTGTCAGACTAGAGACAACCCGAGA100.4488330341113106No Hit
TCAGACTAGAGACAACCCGAGAGCTCAA100.4488330341113106No Hit
TTCCTGTCAGACTAGAGACAACCCGAGA90.4039497307001795No Hit
TTCTTGAGAATATCGGCACGGAGCTCGCA90.4039497307001795No Hit
CGTGTGGTCAC90.4039497307001795No Hit
TTAACGGTTTGGTCACCACACTTGAATGCC80.3590664272890485No Hit
TTGGAGGAAAACTACGGCTCAACCCGTGCT80.3590664272890485No Hit
TCTTGAGAAAATCGGCACGGAGCTCGCATC80.3590664272890485No Hit
TCCAAGGACCCATCTCTCACGTGATTAGCA70.3141831238779174No Hit
TCACAAGTGTCCTGCATTCCTCTCTTGAGA70.3141831238779174No Hit
TCCAGAGTTTGCCCATGTTGCCTCCCAATT70.3141831238779174No Hit
TCTTGAGAATATCGGCACGGAGCTCGCATC70.3141831238779174No Hit
TTGGTCAAGCAGGGCAGGATTAGATAGG70.3141831238779174No Hit
TGCATACGCCCTTCTCTATACTGCCGAAAC70.3141831238779174No Hit
TCATAGAACCTGAACCACGGTAAACCAATG70.3141831238779174No Hit
TTGGAGGAAAACTACGGCTCAACCCGGGCT70.3141831238779174No Hit
TTGGAGGAAAACTACGGCTCAACCCGTGC60.26929982046678635No Hit
TCACATCGTAATCTGTCTCTGACAGAATGTTTGACGCCT60.26929982046678635No Hit
TCGTAATCCGCCTCACCTGTCCCTAAC60.26929982046678635No Hit
TTGACGCCTTCATTCCCTCTGGCAGCGCACC60.26929982046678635No Hit
TTCTTGAGAAAATCGGCACGGAGCTCGC60.26929982046678635No Hit
CCTGTCAGACTAGAGACAACCCGAGAGC60.26929982046678635No Hit
TGACGCCTTCATTCCCTCTGGCAGCGCACC60.26929982046678635No Hit
TAGGTTCTTGAGAAAATCGGCACGGAGCT60.26929982046678635No Hit
TCGTAATCCGCCTCACCTGTCCCTAACTG60.26929982046678635No Hit
TAGTACACCACACCGGGAGACGACATGTC60.26929982046678635No Hit
TAATCACATCGTAATCTGTCTCTGACAGAATGTTTGACGCCT50.2244165170556553No Hit
TTGCCCATGTTGCCTCCCGATTAGTACTC50.2244165170556553No Hit
TCGATACAATCTCTCAACCTGTTGCTTT50.2244165170556553No Hit
TATTTGGTCAAGCAGGGCAGGATTAGAT50.2244165170556553No Hit
TCCAAGGACCCATCTCTCACGTGATTAGC50.2244165170556553No Hit
TCAGATTCGTAATCCGCCTCACCTGTCCC50.2244165170556553No Hit
TTTGGAGGAAAACTACGGCTCAACCCGTGC50.2244165170556553No Hit
TATGGGTATTTGGTCAAGCAGGGCAGGAT50.2244165170556553No Hit
TTTCCTGTCAGACTAGAGACAACCCGAG50.2244165170556553No Hit
TGCATACGCCCTTCTCTATACTGCCGAAACT50.2244165170556553No Hit
TATGACAGACCAGAAACATTGCCCAAACTA40.17953321364452424No Hit
TGCCACCACTGGTGCATACGCCCTTCTC40.17953321364452424No Hit
TGTCAGACTAGAGACAACCCGAGAGCTCAAT40.17953321364452424No Hit
TACGCCCTTCTCTATACTGCCGAAACTGGT40.17953321364452424No Hit
GTTCTTGAGAATATCGGCACGGAGCTCGC40.17953321364452424No Hit
TCCAGAGTTTGCCCATGTTGCCTCCCGATT40.17953321364452424No Hit
TCCTGTCAGACTAGAGACAACCCGAGAGCTCAA40.17953321364452424No Hit
TGTAATCATAGAACCTGAACCACGGTAAACC40.17953321364452424No Hit
TTTTCCTGTCAGACTAGAGACAACCCGAGAGC40.17953321364452424No Hit
TGTTTGACGCCTTCATTCCCTCTGGCAGC40.17953321364452424No Hit
TCATTCCCTCTGGCAGCGCACCTTTTCCT40.17953321364452424No Hit
TGTTTGACGCCTTCATTCCCTCTGGCA40.17953321364452424No Hit
TTTGCCCATGTTGCCTCCCGATTAGTACTCG40.17953321364452424No Hit
TTGGTCAAGCAGGGCAGGATTAGATAGGA40.17953321364452424No Hit
TCCTGTCAGACTAGAGACAACCCGAGAGCTCA30.13464991023339318No Hit
CACCTTTTCCTGTCAGACTAGAGACAACC30.13464991023339318No Hit
AACGGTTTGGTCACCACACTTGAATGCCC30.13464991023339318No Hit
TCCGCCTCACCTGTCCCTAACTG30.13464991023339318No Hit
TTCCTGTCAGACTAGAGACAACCCGAG30.13464991023339318No Hit
TTGGTCGTCACCTCCGAGATCTAGTAGG30.13464991023339318No Hit
CGTAATCCGCCTCACCTGTCCCTAACTGG30.13464991023339318No Hit
TAAGAAACCTGGTGTGTGGGTGGT30.13464991023339318No Hit
AACTACGGCTCAACCCGGGCTTCCCCTGG30.13464991023339318No Hit
TTGAGAAAATCGGCACGGAGCTCGCATC30.13464991023339318No Hit
TGCCCATGTTGCCTCCCGATTAGTACTC30.13464991023339318No Hit
TTGAGAAAATCGGCACGGAGCTCGCATCG30.13464991023339318No Hit
TCTTGAGAATATCGGCACGGAGCTCGCA30.13464991023339318No Hit
GCATTCCTCTCTTGAGA30.13464991023339318No Hit
TAGGTTCTTGAGAATATCGGCACGGAGCT30.13464991023339318No Hit
TGACAGAATGTTTGACGCCTTCATTCCCTC30.13464991023339318No Hit
TCATTGAAACATCTGTCACCTAGTGGTA30.13464991023339318No Hit
TACGCAAAATCTTCTGGGCTAACTTTGGCC30.13464991023339318No Hit
GACACCCTCGTGTTCTCTTAACTCCTTGAAA30.13464991023339318No Hit
CACCTTCACAAGCTTGTCTCCTTCTGGG30.13464991023339318No Hit
TTCGTAATCCGCCTCACCTGTCCCTAACTG30.13464991023339318No Hit
TCCAGAAGAGAATGAACCCCAAGCTTGAA30.13464991023339318No Hit
TGTGTACCACCTTCACAAGCTTGTCTCCTT30.13464991023339318No Hit
TATGACAGACCAGAAACATTGCCCTAACTA30.13464991023339318No Hit
TTCTTGAGAATATCGGCACGGAGCTC30.13464991023339318No Hit
TGACGCCTTCATTCCCTCTGGCAGCGCA30.13464991023339318No Hit
TATCAGTTAGTACACCACACCGGGAGAC30.13464991023339318No Hit
GCGTGTGGTCAC30.13464991023339318No Hit
TGAAATCTACCCCACCACAGAGCAAAGAATC30.13464991023339318No Hit
TAAGAAACCTGGTGTGTGGGTGGTAGATT30.13464991023339318No Hit
GCAGACTATCAGTTAGTACACCACACCGGG30.13464991023339318No Hit
TTTGCCCATGTTGCCTCCCGATTAGTACTC30.13464991023339318No Hit
GCCGGGGTGCTCAGTCTTTAGGGATT30.13464991023339318No Hit
TGACGCCTTCATTCCCTCTGGCAGCGCACCC30.13464991023339318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)