FastQCFastQC Report
Mon 30 Nov 2020
S34.raw.22483.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.22483.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length11-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTACTGGTCCG511.363636363636363No Hit
TGCGTGCAGTCGTTGCAATAGAAGGGTC24.545454545454546No Hit
TATGACTGCGCTCGCGAGCTCAGTTTTCAAAGCTGTTTTTGCACGCGCTC24.545454545454546No Hit
CTGTTGAGCTT24.545454545454546No Hit
TAGGGATACAGCACTCGCACC24.545454545454546No Hit
GTTTTTGCACGCGCTCTGAAAAAGTT24.545454545454546No Hit
AAGCTGTTTTTGCACGTGCTCTGAAAAAGTTGCCGACCCCTGG24.545454545454546No Hit
TGAGGCCGACGAATTTCCTAGGGATACAGC12.272727272727273No Hit
CTGTGTCGCCA12.272727272727273No Hit
CCTGTTGAGCTTG12.272727272727273No Hit
AGTTTTTAAAGCTGTTTTTGCACCCGCTCTGAAAAAGTTGCCGACCC12.272727272727273No Hit
ACCCTGTTGAGCTTGATTCT12.272727272727273No Hit
AAGTTGCCGACCCCTG12.272727272727273No Hit
CAAAATATGACTGCGCCCGCGAGCT12.272727272727273No Hit
GATTCTGTAGGAT12.272727272727273No Hit
TTGTCACATTCGGCCGCTGTACTGGTC12.272727272727273No Hit
ACCTCCTCCGC12.272727272727273No Hit
AATAGCAGACACAGTTTCCCCAGTTCCTTC12.272727272727273No Hit
CGCGCTCTGAAAACGTTGCCGACCCCTG12.272727272727273No Hit
CCTGTTAGAGTTCAAAATATGACTGCGCACGCGAGCTCAGTTTTTAAAGCTGTTTTTGCACGCGCTCTGAAAAAG12.272727272727273No Hit
AACTGTAGCACCAATATCAAACCCCGATCT12.272727272727273No Hit
TCTGTGTCGCCA12.272727272727273No Hit
ACGCGCTCTGAAAAAGTTGCCGACCCCTGATGTAA12.272727272727273No Hit
TCGCTTGATCGCCAACGGTTCTCTGGGT12.272727272727273No Hit
CTCTGAAAAAGTTGCCGACCCCTGGTC12.272727272727273No Hit
TGTTCAAAATATGACTGCGCACGCGAGCTC12.272727272727273No Hit
TTTGTCACATTCGGCCGCTGTACTGG12.272727272727273No Hit
AGTTTTTAAAGCTGTTTTTGCACGCGCTCTGAAAAAGTTGCCGACCCCTGTCATAAGGT12.272727272727273No Hit
TGTTTGAAGAGCCTCGTTTGCGTGCAGTC12.272727272727273No Hit
TGCACGTGCTCTGAAAAAGTTGCCGACCCCT12.272727272727273No Hit
CCTGTTGAGCTT12.272727272727273No Hit
TTAGAGCTGTTTTTGCACGCGCTCTGAAAAAGTTGCCGACCCCT12.272727272727273No Hit
TGACTGCGCACGCGAGCTCAGTTTTTAAAGCTGTTTTTGCACGCGCTCTG12.272727272727273No Hit
TCTTGATTCTGTA12.272727272727273No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)