FastQCFastQC Report
Mon 30 Nov 2020
S34.raw.22483.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.22483.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263
Sequences flagged as poor quality0
Sequence length11-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGAAAAGCTTACTCGGCCTGTGCATC5420.53231939163498No Hit
TGCAAGCAGTGAAGCCGCAGCCTTACGGAC155.7034220532319395No Hit
TAGGAAATTCGTCGGCCTCACAAGTGTGG93.4220532319391634No Hit
TCGGCAACTTTTTCAGAGCGCGTGCGAAA93.4220532319391634No Hit
TACAACTGTGGAACGGGGCGATTGGGA62.2813688212927756No Hit
TCAGGGGTCGGCAACTTTTTCAGAGCGC51.9011406844106464No Hit
TCAAAGAAAAGCTTACTCGGCCTGTGCATCA41.520912547528517No Hit
TAAGGATGCAGCATCCAATGCAACGTGTAA41.520912547528517No Hit
GCAAGCAGTGAAGCCGCAGCCTTACGGAC41.520912547528517No Hit
TAAATTTTGCGAGGTAGACGTAACGTCGGC41.520912547528517No Hit
TACAACTGTGGAACGGGGCGATTGGGACG41.520912547528517No Hit
TAGGAAATTCGTCGGCCTCACAAGTGTGGA31.1406844106463878No Hit
TCGGCCAAGTCTACACCACCATATTTGTATT31.1406844106463878No Hit
TCAAACTCCACCTTACCTTTTCGTCAATGG31.1406844106463878No Hit
TGAGCTCGCGGGCGCAGTCATATTTTGAAAAA31.1406844106463878No Hit
TCAAGCTCAACAGACCCTTCTATTGCAAC31.1406844106463878No Hit
TACAACTGTGGAACGGGGCGATTGGGAC31.1406844106463878No Hit
TCCCTAGGAAATTCGTCGGCCTCACAAGT31.1406844106463878No Hit
AAGAAAAAGATCGGCCATGGCAGGCA31.1406844106463878No Hit
TCGGCAACTTTTTCAGAGCGCGTGCAAAAAC31.1406844106463878No Hit
TCTGCTCGTACTGAAGGAACTGGGGAAACT31.1406844106463878No Hit
TTTTCAGAGCGCGTGCGAAAACAGCTTT31.1406844106463878No Hit
TTTTGCGAGGTAGACGTAACGTCGTCGGC31.1406844106463878No Hit
TGCGAGGTAGACGTAACGTCGTCGGCTT20.7604562737642585No Hit
TGCTGTATCCCTAGGAAATTCGTCGGCCTC20.7604562737642585No Hit
TGCTCGTACTGAAGGAACTGGGGAAACTGT20.7604562737642585No Hit
TAGTCAAGTCCAAGCCTACCTAGACACA20.7604562737642585No Hit
TCCTACAGAATCAAGCTCAACAGACCCTT20.7604562737642585No Hit
TACAACTGTGGAACGGGGCGATTGGG20.7604562737642585No Hit
TGCTGTATCCCTAGGAAATTCGTCGGCC20.7604562737642585No Hit
TACGTAGCGGAGGAGGTGCGAGTGCTGT20.7604562737642585No Hit
TTATACTTCATGCGGTACAGGGCAATCATT20.7604562737642585No Hit
TTATTACTCCTTTGTCGTATATACCTGTA20.7604562737642585No Hit
TCAGGGGTCGGCAACTTTTTCAGAGCGCG20.7604562737642585No Hit
TACCAGGGGTCGGCAACTTTTTCAGAGC20.7604562737642585No Hit
ATCAGGGGTCGGCAAC20.7604562737642585No Hit
AGCAAACAAGAAAAAGATCGGCCATGGC20.7604562737642585No Hit
TGTATTGTTCCCTATCATCGAGCTTCAGG20.7604562737642585No Hit
TCGTACTGAAGGAACTGGGGAAACTGTG20.7604562737642585No Hit
TGCTGTATCCCTAGGAAATTCGTCGGCCT20.7604562737642585No Hit
TCATGCGGTACAGGGCAATCATTCTATCGGCCAAG20.7604562737642585No Hit
TACAACTGTGGAACGGGGCGATTGGGACGT20.7604562737642585No Hit
TCAGGAACGCCTATACGTAGCGGAGGAGG20.7604562737642585No Hit
AATCCTACAGAATCAAGCTCAACAGACCCT20.7604562737642585No Hit
TAGGAAGCGTAAACCCAGAGAACCGTTGGCG20.7604562737642585No Hit
ATCATTCTATCGGCCAAGTCTACACCACC10.38022813688212925No Hit
ATCCCTAGGAAATTCGTCGGCCTCACAAGA10.38022813688212925No Hit
TCGTATATACCTGTAGACTATTTGCCAT10.38022813688212925No Hit
TACGTAGCGGAGGAGGTGCGAGTGCTGTAA10.38022813688212925No Hit
TGAAGCCGCAGCCTTACGGACCAGTACAG10.38022813688212925No Hit
TCTATCGGCCAAGTCTACACCACCATATT10.38022813688212925No Hit
ATCCCTAGGAAATTCGTCGGCCTCACAAGT10.38022813688212925No Hit
TTCGTCAATGGTGTGCAAGCAGTGAAGCCGC10.38022813688212925No Hit
TACGAATACGAACCTCACTTCGGAATTGT10.38022813688212925No Hit
TCTGCTCGTACTGAAGGAACTGGGGAAACTT10.38022813688212925No Hit
AAGAAAAAGATCGGCCATGGCAGG10.38022813688212925No Hit
TACGTAGCGGAGGAGGTGCGAGTGCTGTA10.38022813688212925No Hit
AAATACAACTG10.38022813688212925No Hit
TTGGGAGTGAGATCGGGGTTTGATATTG10.38022813688212925No Hit
AGCAAACAAGAAAAAGATCGGCCATGGCAGG10.38022813688212925No Hit
TCCTACAGAATCAAGCTCAACAGACCCTTC10.38022813688212925No Hit
TATTGCAACGACTGCACGCAAACGAGGCTC10.38022813688212925No Hit
GAGGACATGGATACCAGCCACAATGTCGGC10.38022813688212925No Hit
TGTGTACTATTCATTAGATCAGGGGTCGGT10.38022813688212925No Hit
CTATTCCAGGGGTCGGCAACTTTTTCAGAGCGCGTGCAAAAACAGCTTTAAAAACTGAGCTCG10.38022813688212925No Hit
TTATACTTCATGCGGTACAGGGCAATCAT10.38022813688212925No Hit
TCGGCAACTTTTTCAGAGCGCGTGCGAAAAC10.38022813688212925No Hit
TGTGTACTATTCATTAGATCAGGGGTCGGC10.38022813688212925No Hit
TAGAAGAGCAAACAAGAAAAAGATCGGCC10.38022813688212925No Hit
AGGAAGCGTAAACCCAGAGAACCGTTGGC10.38022813688212925No Hit
TTCGACACAAATGGAGGACATGGATACCAGCC10.38022813688212925No Hit
TACATCAGGGGTCGGCAACTTTTTCAGAGC10.38022813688212925No Hit
TACAACTGTGGAACGGGGCGATTGGGAA10.38022813688212925No Hit
TTCCACCGAAGATCAAAATTTTGTAAACTGT10.38022813688212925No Hit
TTTGTCTGATATGAGTATGGTGGCGTT10.38022813688212925No Hit
TCGTACTGAAGGAACTGGGGAAACTGTGT10.38022813688212925No Hit
TTATCAGAGCGCGTGCAAAAACA10.38022813688212925No Hit
TAAGTCTGCTCGTACTGAAGGAACTGGGG10.38022813688212925No Hit
TAGGAAGCGTAAACCCAGAGAACCGTTGGC10.38022813688212925No Hit
TCGTGTCAGGTGAAAATATTGGGAGTGAG10.38022813688212925No Hit
AACTTTTTCAGAGCGCGTGCGAAAACAGC10.38022813688212925No Hit
TCTGCTGCTAAGTCTGCTCGTACTGAAGG10.38022813688212925No Hit
TACAGAATCAAGCTCAACAGACCCTTCTAT10.38022813688212925No Hit
TTGGGACGCCGACACGAAAAAGAACG10.38022813688212925No Hit
TGCTACGTTACCCCCTAATACTTGGATT10.38022813688212925No Hit
TACAACTGTGGAACGGAGCGATTGGGACGC10.38022813688212925No Hit
TATCCCTAGGAAATTCGTCGGCCACACAAG10.38022813688212925No Hit
TCAAACTCCACCTTACCTTTTCGTCAATG10.38022813688212925No Hit
GAAATTCGTCGGCCTCACAAGTGTGGAC10.38022813688212925No Hit
GACACAGGTGG10.38022813688212925No Hit
TTCTGCTGCTAAGTCTGCTCGTACTGAAGG10.38022813688212925No Hit
ACGATCCTCTTCTACAAATCCTACAGAAT10.38022813688212925No Hit
AAAGCACCGGATTCTATGTATTGTTCCCTA10.38022813688212925No Hit
TACGTAGCGGAGGAGGTGCGAGTGCTG10.38022813688212925No Hit
TGCTGTATCCCTAGGAAATTCGTCGGCCC10.38022813688212925No Hit
TCTATTGCAACGACTGCACGCAAACGAGG10.38022813688212925No Hit
TACATAAGAAGCGCAATGGTTCCACCTAGG10.38022813688212925No Hit
TCTGCTCGTACTGAAGGAACTGGGGAAA10.38022813688212925No Hit
TGCACGCAAACGAGGCTCTTCAAACACAGG10.38022813688212925No Hit
TTGGGACGCCGACACGAAAAAGAACGTCT10.38022813688212925No Hit
TCTAAAGCAGGGGTCGGCAACTTTTTCAGAGCGCGTGCAAAAACAGCTTT10.38022813688212925No Hit
TCCACCGAAGATCAAAATTTTGTAAACTGTT10.38022813688212925No Hit
TTTAGCAAATCTCGTGTGTGGTACTCAT10.38022813688212925No Hit
CACAGGAACGC10.38022813688212925No Hit
TTGGGAGTGAGATCGGGGTTTGATATTGGT10.38022813688212925No Hit
AGGGGTCGGCAACTTTTTC10.38022813688212925No Hit
TTTAGCAAATCTCGTGTGTGGTACTCATA10.38022813688212925No Hit
TTAAATTTTGCGAGGTAGACGTAACGTCGGT10.38022813688212925No Hit
TCCTACGGAATCAAGCTCAACAGACCCTTC10.38022813688212925No Hit
ACAACTGTGGAACGGGGCGATTGGGACGC10.38022813688212925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)