FastQCFastQC Report
Mon 30 Nov 2020
S34.raw.2812.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.2812.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105
Sequences flagged as poor quality0
Sequence length11-75
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTGAAGAAACTGTGCCCGGAGA98.571428571428571No Hit
CCCCTGTGAAGAAACTGTGCCCGGAGA87.6190476190476195No Hit
GTCCGACGGCCCCTG65.714285714285714No Hit
TTACCGTCATCGCAGAACACCACGACCACGTCCACG54.761904761904762No Hit
CCTGTGAAGAAACTGTGCCCGGAGAA43.8095238095238098No Hit
TATCTTCTCCCATGACCAATTTCATGCACATAGGGCACATGAACTC32.857142857142857No Hit
TAGGCAGGCAAGTAGAATGGTATAAAGCT32.857142857142857No Hit
TTCATCGAGCAGCAGCGTGGTCGCGTCAGC32.857142857142857No Hit
AGAACAATTACTCGAGTTCGTTCGGAACCACCCTGCTGCGCCGTTGAAGC32.857142857142857No Hit
TCATCGCAGAACACCACGACCACGTCCACG32.857142857142857No Hit
GACCGACGGCCCCT21.9047619047619049No Hit
ATTACTCGAAATGTGTAGGTCAACCGAAGGAAGTCTACTGGAAGCAA21.9047619047619049No Hit
CGGGACTTCAAGAAAGGCGAGATGGGCAGTTTAAGCGCCCCAGAAATACGGGAGA21.9047619047619049No Hit
CCTGTGAAGAAACTGTGCCCGGAG21.9047619047619049No Hit
CCCCTGTGAAGAAACTGTGCCCGGAGAA21.9047619047619049No Hit
CGAGTTCGTTCGGAACCACCCTGCTGCGCCGTTGAAGCT21.9047619047619049No Hit
TCTACCAGCAAACCGCCAATTATGGATGTCGAGTCGGACGAAGAGTTATTCCCCGGCATGAGT21.9047619047619049No Hit
CATCGCAGAACACCACGACCACGTCCACGTTATACACGACTGTTCCTATCAATCCAACCAGTGCAGATGTGCCCA21.9047619047619049No Hit
CATCGCAGAACACCACGACCACGTCCACG21.9047619047619049No Hit
TAATAACGCATTTACCGTCATCGCAGAACACCACGACCACGTCCACGTTATACACG21.9047619047619049No Hit
TCTTAGGCAGGCAAGTAGAATGGTATAAAG21.9047619047619049No Hit
TTTACCGTCATCGCAGAACACCACGACCACGTCCACGTTATACACG10.9523809523809524No Hit
TTAATAGGATT10.9523809523809524No Hit
CCCCTGTGAAGAAACTGTGCCCGGAGAAAGC10.9523809523809524No Hit
CCTGTGAAGAAACTGTGCCCGGAGAAAGC10.9523809523809524No Hit
TCCCAGACCGACGGCCCCTGTGAAGAAACTGTGCCCGGAGTAG10.9523809523809524No Hit
TATCGCGTAGTCCTCTTGAAAGTATTTCT10.9523809523809524No Hit
GACCGACGGCCCC10.9523809523809524No Hit
AGACCGACGGCCCCTGT10.9523809523809524No Hit
CCTGTGAAGAAACTGTGCCCGGAGAAT10.9523809523809524No Hit
ATCGAAGTGGACT10.9523809523809524No Hit
CTGTGAAGAAACTGTGCCCGGAGAAAGC10.9523809523809524No Hit
ACAATTACTCGAGTTCGTTCGGAACCACCCTGCTGCGCCGTT10.9523809523809524No Hit
GACTATAGGTCTGCTGCCCCCACTATT10.9523809523809524No Hit
CCTGTGAAGAAACTGTGCCCGGAGAACC10.9523809523809524No Hit
TTGGGTCAATA10.9523809523809524No Hit
AACACCACGACCACGTCCACGTTATACACGACTG10.9523809523809524No Hit
TTTGTTTGTGTA10.9523809523809524No Hit
ACTTCAAGAAAGGCGAGATGGGCAGTTTAAGCGCCCCAGAAATACGGG10.9523809523809524No Hit
ATCAAGCCCCACC10.9523809523809524No Hit
TCATCGCAGAACACCACGACCACGTCCACGTTATACACGACTG10.9523809523809524No Hit
TCCGGAGCCAAAGTGCAAGTGCATTTGACT10.9523809523809524No Hit
CTTCGGCGTCT10.9523809523809524No Hit
CCCCTGTGAAGAAACTGTGCCCGGAG10.9523809523809524No Hit
TACGGGAGAACAATTACTCGAGTTCGTTCGGAACCACCCTGCTGCGCCGTTGAAGC10.9523809523809524No Hit
CCTGTGAAGAAACTGTGCCCGGA10.9523809523809524No Hit
GAGATTACTACGCAACAATTGACCCTG10.9523809523809524No Hit
TATTCCCCGGCATGAATTCACAATTAAGT10.9523809523809524No Hit
AGGAAGTCTACTGGAAGCAAGCAACT10.9523809523809524No Hit
AAAGTTATTAATAACGCATTTACCGTCATCGCAGAACACCACGACCACGTCCACGTTATACACGACTGTTCCT10.9523809523809524No Hit
CACTCTCAGTG10.9523809523809524No Hit
AACGCATTTACCGTCATCGCAGAACACCACGACCACGTCCACGTTATACACG10.9523809523809524No Hit
CCTGTGAAGAAACTGTGCCCGGAGAAAGCATCCGGAGCCAAAGTGCAAGTGCATTTGACTCCCGATCAGCA10.9523809523809524No Hit
TCCCCTGTGAAGAAACTGTGCCCGGAG10.9523809523809524No Hit
GACAGTGCAGATGTG10.9523809523809524No Hit
CCGACGGCCCCTGTGAAGAAACTGTGCC10.9523809523809524No Hit
ACTGTACTCAACAACAGAGCAATTGAATTGGCGGCT10.9523809523809524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)