FastQCFastQC Report
Mon 30 Nov 2020
S34.raw.28821.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.28821.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303
Sequences flagged as poor quality0
Sequence length11-75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGAAGAACTCTCGTGCTTGCGCTGGG196.270627062706271No Hit
TAATCGCTGTACATCTCTCTGAACCGTGG123.9603960396039604No Hit
TTTCTTTACCAAGTACCCCTGAACCTTCA103.3003300330033No Hit
TAGAACTCGTGAATCGCAGTGGGCTGCGG82.6402640264026402No Hit
TAGTCTCTTCGCAGGAGCTGGACCGCCT82.6402640264026402No Hit
TCGCTGTACATCTCTCTGAACCGTGGGA72.31023102310231No Hit
GTGTCGGAGCCA72.31023102310231No Hit
TTACCAAGTACCCCTGAACCTTCAGGCCCA61.9801980198019802No Hit
TACTAGTCTCTTCGCAGGAGCTGGACCGCC61.9801980198019802No Hit
TCTTTGAAGAACTCTCGTGCTTGCGCTGG61.9801980198019802No Hit
TGAAGAACTCTCGTGCTTGCGCTGGGGT51.65016501650165No Hit
TGCGATATGGTTTTTCAAGGGAGATGGGC51.65016501650165No Hit
TTGAAGAACTCTCGTGCTTGCGCTGGG51.65016501650165No Hit
TACTCCAACCTCCTGAGAACATCTACGCATC41.3201320132013201No Hit
AACATCGACATG41.3201320132013201No Hit
TCTTCCAACTTGACCTCCTCTGGTACTTGA41.3201320132013201No Hit
TACGTCTGTATGACTCAGCCCCGTACATA41.3201320132013201No Hit
AACATCGACATGT41.3201320132013201No Hit
TTACCAAGTACCCCTGAACCTTCAGGCCC41.3201320132013201No Hit
TACCAAGTACCCCTGAACCTTCAGGCCCA41.3201320132013201No Hit
TAGAACTCGTGAATCGCAGTGGGCTGCGGT30.9900990099009901No Hit
CATCTAGGGGCAGAACTTTTTTCATTAGA30.9900990099009901No Hit
TGAACACCCATCTCCTCTAGCAGCTTCCCGGC30.9900990099009901No Hit
TCAGCATCTACATCGATAACTACCCCGTC30.9900990099009901No Hit
TTTACCAAGTACCCCTGAACCTTCAGGCCCA30.9900990099009901No Hit
TACTAGTCTCTTCGCAGGAGCTGGACCGCCT30.9900990099009901No Hit
TGCGCTAGGTACTAGTCTCTTCGCAGG30.9900990099009901No Hit
TGATCTTCCAACTTGACCTCCTCTGGTACT30.9900990099009901No Hit
TGAACACCCATCTCCTCTAGCAGCTTCCCGG30.9900990099009901No Hit
TTGAAGAACTCTCGTGCTTGCGCTGGGGT30.9900990099009901No Hit
TTGCTGTACATCTCTCTGAACCGTGGGA30.9900990099009901No Hit
TTTGAAGAACTCTCGTGCTTGCGCTGGGG30.9900990099009901No Hit
TCTACATCGATAACTACCCCGTCATCTGG30.9900990099009901No Hit
TAGAACTCGTGAATCGCAGTGGCTGC30.9900990099009901No Hit
TGATCTTCCAACTTGACCTCCTCTGGTACTT30.9900990099009901No Hit
GAACATCGACATGT30.9900990099009901No Hit
TGATTAACCTACCATTCTGAGACAGTTGAT30.9900990099009901No Hit
TCCAATAGAACTCGTGAATCGCAGTGGGC30.9900990099009901No Hit
TAGAACTCGTGAATCGCAGTGGGCTGC30.9900990099009901No Hit
TCATGGTACCGCTCGGCTCAACGATCTT20.6600660066006601No Hit
ATAGAACTCGTGAATCGCAGTGGGCTGC20.6600660066006601No Hit
CTCTGCCACCA20.6600660066006601No Hit
TCTTTTCCAGGCCTTGCATCAATGATGCT20.6600660066006601No Hit
TGAAGAACTCTCGTGCTTGCGCTGGGG20.6600660066006601No Hit
TGAACACCCATCTCCTCTAGCAGCTTCCCGC20.6600660066006601No Hit
TTAGCAGTCATGGTACCGCTCGGCTCAACG20.6600660066006601No Hit
TGGGTGAACACCCATCTCCTCTAGCAGCTTC20.6600660066006601No Hit
TACCAAGTACCCCTGAACCTTCAGGCCCAAT20.6600660066006601No Hit
TCCTCTAGCAG20.6600660066006601No Hit
TTCACGATATCAACTTGTCCCTTTGACACCGG20.6600660066006601No Hit
TTTCTTTACCAAGTACCCCTGAACCTTCAGGCC20.6600660066006601No Hit
TTCGAGCAAACATCTAGGGGCAGAACTT20.6600660066006601No Hit
TTGAAGAACTCTCGTGCTTGCGCTGGGG20.6600660066006601No Hit
TGAACATCGACATGT20.6600660066006601No Hit
TACCAAGTACCCCTGAACCTTCAGGCCC20.6600660066006601No Hit
TTTGAAGAACTCTCGTGCTTGCGCTGG20.6600660066006601No Hit
TCTTTGAAGAACTCTCGTGCTTGCGCTGGG20.6600660066006601No Hit
TGAAGAACTCTCGTGCTTGCGCTGG20.6600660066006601No Hit
TCCAGATGTCGAGATGAACAACTCTTTGTC20.6600660066006601No Hit
TGTCCCTTTGACACCGGACTTAATGCGTC20.6600660066006601No Hit
TTTGAAGAACTCTCGTGCTTGCGCCGGG10.33003300330033003No Hit
TTTCTTTACCAAGTACCACTGAACCTTCA10.33003300330033003No Hit
TGTATCCAGATGTCGAGATGAACAACTCTTTGTCGGAG10.33003300330033003No Hit
TCCAACCTCCTGAGAACATCTACGCATCTT10.33003300330033003No Hit
TGAAGAACTCTCGTGCTTGCGCTGGG10.33003300330033003No Hit
TCCGATACCCAGGTGATCTTCTTCTGATC10.33003300330033003No Hit
TTTGTATCCAGATGTCGAGATGAACAACT10.33003300330033003No Hit
TCATTAGAGGACCAAAGGCTGTCTCATCT10.33003300330033003No Hit
TCAGCATCTACATCGATAACTACCCCGTCA10.33003300330033003No Hit
TAGAACTCGTGAATCGCAGTGGGCTG10.33003300330033003No Hit
CTGGACCGCCTCTACTCTCGACAGCATAGGT10.33003300330033003No Hit
TAATGCGTCTGGTTTATTGTTGTAAAAGTT10.33003300330033003No Hit
TCACGATATCAACTTGTCCCTTTGACACCG10.33003300330033003No Hit
TACTCCAACCTCCGGAGAACATCTACGCATC10.33003300330033003No Hit
TGTATCCAGATGTCGAGATGAACAACTCT10.33003300330033003No Hit
TCCAACCTCCTGAGAACATCTACGCATCTTT10.33003300330033003No Hit
GAACACCCATCTCCTCTAGCAGCTTCCCGGC10.33003300330033003No Hit
TTTGTATCCAGATGTCGAGATGAACAACTC10.33003300330033003No Hit
CTTCCAACTTGACCTCCTCTGGTACTTGA10.33003300330033003No Hit
TCCAACCTCCTGAGAACATCTACGCATCT10.33003300330033003No Hit
TCGAGATGAACAACTCTTTGTCGGAG10.33003300330033003No Hit
ACTAGTCTCTTCGCAGGAGCTGGACCGCCT10.33003300330033003No Hit
TGTATCCAGATGTCGAGATGAACAACTCTTT10.33003300330033003No Hit
TATGACTCAGCCCCGTACATACAAGTCCAT10.33003300330033003No Hit
TTCATACGTCTGTATGACTCAGCCCCGT10.33003300330033003No Hit
TTTAACTTGCCCTGCTTCTCTCACAGACACAG10.33003300330033003No Hit
TTTGGACCGCCTCTA10.33003300330033003No Hit
ATCGCTGTACATCTCTCTGAACCGTGG10.33003300330033003No Hit
TGTCGCAAACCCTCTTTCTCCTCCAATAGGTC10.33003300330033003No Hit
AGATGTTGGGTT10.33003300330033003No Hit
TCTGGTCGTAAACAAAAAGTCCGTCTCCC10.33003300330033003No Hit
TAAGACTTTCACGATATCAACTTGTCCCTT10.33003300330033003No Hit
TCGAGCAAACATCTAGGGGCAGAACTTTT10.33003300330033003No Hit
TAGGGGCAGAACTTTTTTCATTAGAGGACC10.33003300330033003No Hit
TGAAGAACTCTCGTGCTTGCGCTGGGGTTATGATAGTGAAATCCGATACCCAGGTGATCTT10.33003300330033003No Hit
GAGGACCAAAGGCTGTCTCATCTGCGATAGG10.33003300330033003No Hit
TAGAGGACCAAAGGCTGTCTCATCTGCGA10.33003300330033003No Hit
TTCACGATATCAACTTGTCCCTTTGACACCG10.33003300330033003No Hit
TGTCAGTGGCGAGCCTGATTCATACGTC10.33003300330033003No Hit
TCCCACCACCATCTACTCCAACCTCCTGAG10.33003300330033003No Hit
TGTTAGCAGTCATGGTACCGCTCGGCT10.33003300330033003No Hit
TTCAAGGGAGATGGGCTATTCACTAGGCA10.33003300330033003No Hit
TGTCGAGATGAACAACTCTTTGTCGGAGCC10.33003300330033003No Hit
ATAGAACTCGTGAATCGCAGTGGGC10.33003300330033003No Hit
TGCGATATGGTTTTTCAAGGGAGATGG10.33003300330033003No Hit
TACTCCAACCTCCTGAGAACATCTACGCAT10.33003300330033003No Hit
TGATTGTTAGCAGTCATGGTACCGCTCGGC10.33003300330033003No Hit
TTTGTCAAGTGGCGAGCCTGATTCATACGT10.33003300330033003No Hit
TCATTTATTCCTAGGACCTCTTTAGCTT10.33003300330033003No Hit
TCCAGATGTCGAGATGAACAACTCTTTGT10.33003300330033003No Hit
TAGGTTGCATCTTTTCCAGGCCTTGCAT10.33003300330033003No Hit
ATAGAACTCGTGAATCGCAGTGGGCTGCGGT10.33003300330033003No Hit
CTCCAACCTCCGGAGAACATCTACGCATC10.33003300330033003No Hit
TACGTCTGTATGACTCAGCCCCGTACATAC10.33003300330033003No Hit
TTCCAACTTGACCTCCTCTGGTACTTGAGGC10.33003300330033003No Hit
TAGTCTCTTCGCAGGAGCTGGACCGCC10.33003300330033003No Hit
TCCAACTTGACCTCCTCTGGTACTTGAGGC10.33003300330033003No Hit
TAGGGTATGCAGCTGCTTCGCACCTCTTCTGCATCACATTGTCATACTGGATGGAGTTAGCAAATGTTTTTGTCA10.33003300330033003No Hit
NTACCAAGTACCCCTGAACCTTCAGGCCC10.33003300330033003No Hit
TAGAGGACCAAAGGCTGTCTCATCTGCGAA10.33003300330033003No Hit
GATGTTGGGTT10.33003300330033003No Hit
TCTGTCCATGTCGCAAACCCTCTTTCTCCT10.33003300330033003No Hit
TGTATCCAGATGTCGAGATGAACAACTC10.33003300330033003No Hit
TCCATGTCGCAAACCCTCTTTCTCCTCCA10.33003300330033003No Hit
TGCTAGCTCTGCCACCAGAGTAGTTGCGGC10.33003300330033003No Hit
TCTACATCGATAACTACCCCGTCATATGG10.33003300330033003No Hit
TCTAGGGGCAGAACTTTTTTCATTAGAGGAC10.33003300330033003No Hit
TCTGCGATATGGTTTTTCAAGGGAGATGGG10.33003300330033003No Hit
TTTCTTTACCAAGTACCCCCGAACCTTCA10.33003300330033003No Hit
TGACTCAGCCCCGTACATACAAGTCCATG10.33003300330033003No Hit
TCGGAGCCATCAACATGCAAAGTTAAGCCT10.33003300330033003No Hit
TCAACATGCAAAGTTAAGCCTGGCTCAAGC10.33003300330033003No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)