FastQCFastQC Report
Mon 30 Nov 2020
S34.raw.2997.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.2997.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3111
Sequences flagged as poor quality0
Sequence length11-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCCGTCTGCGCACTGGATGAAACCGT1765.657344905175185No Hit
TTCTTGAAGTCCCGAGTTATCGCTTTCTG1484.757312761170042No Hit
TCCCGAGTTATCGCTTTCTGTAATTGTCTC802.571520411443266No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACATC652.0893603342976537No Hit
TACTTAATTGTGAACTCATGCCGGGGAA652.0893603342976537No Hit
TAACTCTTCGTCCGACTCGACATCCATA591.8964963034394087No Hit
TGCTTCCAGTAGACTTCCTTCGGTTGACCT571.832208293153327No Hit
TCCATAATTGGCGGTTTGCTGGTAGATA521.6714882674381228No Hit
TCTTGAAGTCCCGAGTTATCGCTTTCTG511.639344262295082No Hit
TTCAACGGCGCAGCAGGGTGGTTCCGAAC491.5750562520090001No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACA461.4786242365798778No Hit
TCGTGTATAACGTGGACGTGGTCGTGGT371.1893281902925106No Hit
TCGTGTATAACGTGGACGTGGTCGTGGTG361.1571841851494697No Hit
TAGATATGGGCACATCTGCACTGGTTGGAT351.1250401800064287No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGAGT331.0607521697203472No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGAG310.9964641594342655No Hit
TCCTCCCATACTTGCGCTTGCTGATCGGG270.8678881388621021No Hit
TACTTAATTGTGAACTCATGCCGGGGAAT260.8357441337190614No Hit
TCCGGGCACAGTTTCTTCACAGGGGACGTC250.8036001285760206No Hit
TGCCCCGTCTGCGCACTGGATGAAACCG240.7714561234329798No Hit
TGCTTGCTTCCAGTAGACTTCCTTCGGT230.7393121182899389No Hit
TGCCCCGTCTGCGCACTGGATGAAACC230.7393121182899389No Hit
TCATCTCCAGATTCGCATGCTGACGCGAC230.7393121182899389No Hit
TAATTGGCGGTTTGCTGGTAGATATGGGC220.7071681131468981No Hit
TTTGCTGGTAGATATGGGCACATCTGCACTG220.7071681131468981No Hit
TGCTGATCGGGAGTCAAATGCACTTGCACT200.6428801028608165No Hit
TCTCCATAATCCATTAGTGACCAAGCCGCC190.6107360977177756No Hit
TATGGGCACATCTGCACTGGTTGGATT190.6107360977177756No Hit
TTCTGTAATTGTCTCAACAGTTGCCCCGTC180.5785920925747349No Hit
TTCACAGGGGCCGTCGGTCTGGGACACTC170.546448087431694No Hit
TGCTGGTAGATATGGGCACATCTGCACT160.5143040822886531No Hit
TTGCCCCGTCTGCGCACTGGATGAAACCG160.5143040822886531No Hit
TGACCAAGCCGCCAATTCAATTGCTCTGTT160.5143040822886531No Hit
TTGCCCCGTCTGCGCACTGGATGAAACCGT150.4821600771456124No Hit
TAAGACTGTAGTAAAGTTCTTTGTGGCGGT140.4500160720025715No Hit
TGAAGTCCCGAGTTATCGCTTTCTGTAATT140.4500160720025715No Hit
TATGGGCACATCTGCACTGGTTGGATTG130.4178720668595307No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACAT130.4178720668595307No Hit
TGTCTCAACAGTTGCCCCGTCTGCGCACT130.4178720668595307No Hit
TCTGTAATTGTCTCAACAGTTGCCCCGTC130.4178720668595307No Hit
TGAAATATGAGTTTCAACGGCGCAGCAGGG130.4178720668595307No Hit
TAGTAATTGTTCTCCCGTATTTCTGGGGC120.3857280617164899No Hit
TTGGCGGTTTGCTGGTAGATATGGGCAC120.3857280617164899No Hit
TGCTTGCTTCCAGTAGACTTCCTTCGGTT110.35358405657344905No Hit
TCACAGGGGCCGTCGGTCTGGGACACTC110.35358405657344905No Hit
TCCGGGCACAGTTTCTTCACAGGGGCCGTC110.35358405657344905No Hit
TCGTCCGACTCGACATCCATAATTGGCGGT110.35358405657344905No Hit
TTCGAGCTTCATTACCAAATCGTGGGAT100.32144005143040827No Hit
TGCTTCCAGTAGACTTCCTTCGGTTGACC100.32144005143040827No Hit
TCGACATCCATAATTGGCGGTTTGCTGGT100.32144005143040827No Hit
TTCGTCCGACTCGACATCCATAATTGGCGGT100.32144005143040827No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGA100.32144005143040827No Hit
TCATCTCCAGATTCGCATGCTGACGCGACC90.28929604628736744No Hit
TATGGGCACATCTGCACTGGTTGGATTGAT90.28929604628736744No Hit
TGCTGGTAGATATGGGCACATCTGCACTG90.28929604628736744No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTGA90.28929604628736744No Hit
TCGGAAGAAGAGCATCCCATACTTGTTGAGT90.28929604628736744No Hit
TATGAGCTTCAACGGCGCAGCAGGGTGG90.28929604628736744No Hit
TTCGAGCTTCATTACCAAATCGTGGGATC90.28929604628736744No Hit
TCACAGGGGACGTCGGTCTGGGACACTC80.25715204114432655No Hit
TTGTAGATCGTCTGGAACTTGGTAAGAGA80.25715204114432655No Hit
TTCCTCCCATACTTGCGCTTGCTGATCGG80.25715204114432655No Hit
TGCTGGTAGATATGGGCACATCTGCACTGG80.25715204114432655No Hit
TCTTCACAGGGGACGTCGGTCTGGGACACTC80.25715204114432655No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTG80.25715204114432655No Hit
TTTGCTGGTAGATATGGGCACATCTGCACT80.25715204114432655No Hit
TCTTCACAGGGGACGTCGGTCTGGGACACT80.25715204114432655No Hit
TAAGTCTTCGTCCGACTCGACATCCATA80.25715204114432655No Hit
TTGTCTCAACAGTTGCCCCGTCTGCGCAC80.25715204114432655No Hit
TCCGAACGAACTCGAGTAATTGTTCTCCCG70.22500803600128574No Hit
TGCGCTTGCTGATCGGGAGTCAAATGCACT70.22500803600128574No Hit
TCTCAACAGTTGCCCCGTCTGCGCACTGG70.22500803600128574No Hit
TTGTCTCAACAGTTGCCCCGTCTGCGCACT70.22500803600128574No Hit
TAGATATGGGCACATCTGCACTGGTTGGA70.22500803600128574No Hit
TCTTCACAGGGGCCGTCGGTCTGGGACACTC70.22500803600128574No Hit
TAGTGACCAAGCCGCCAATTCAAT70.22500803600128574No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTT70.22500803600128574No Hit
TTGGCTTTCGAAGGAGACGGATGTGTGCC70.22500803600128574No Hit
TCGACATCCATAATTGGCGGTTTGCTGGTA70.22500803600128574No Hit
TCCGGGCACAGTTTCTTCACAGGGGACG70.22500803600128574No Hit
TATCGCTTTCTGTAATTGTCTCAACAGTT60.19286403085824494No Hit
TCTTGAAGTCCCGAGTTATCGCTTTCTGT60.19286403085824494No Hit
TCGGTCTGGGACACTCTTGGAAGGTCAGC60.19286403085824494No Hit
TACTTAATTGTGAATTCATGCCGGGGAA60.19286403085824494No Hit
TGTCTCAACAGTTGCCCCGTCTGCGCACTG60.19286403085824494No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCG60.19286403085824494No Hit
TGAAATATGAGCTTCAACGGCGCAGCAGGG60.19286403085824494No Hit
TCCGGGCACAGTTTCTTCACAGGGGCCGT60.19286403085824494No Hit
TGTTGTAGATCGTCTGGAACTTGGTAAGA60.19286403085824494No Hit
ACTGCCCATCTCGCCTTTCTTGAAGTCCCG60.19286403085824494No Hit
TATATTGTTGTAGATCGTCTGGAACTTGGT60.19286403085824494No Hit
TCCTTCGGTTGACCTACACATTTCGAGTA60.19286403085824494No Hit
TGAAATAGTGGGGGCAGCAGACCTATAGTCACGCCAGT60.19286403085824494No Hit
TTCATCTCCAGATTCGCATGCTGACGCGAC60.19286403085824494No Hit
TCCAAGTTGCTTGCTTCCAGTAGACTTCCT60.19286403085824494No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACT60.19286403085824494No Hit
TTCTTCACAGGGGCCGTCGGTCTGGGACA60.19286403085824494No Hit
TGTTCCTCCCATACTTGCGCTTGCTGATCGG50.16072002571520413No Hit
TCTGCACTGGTTGGATTGATAGGAACAGTC50.16072002571520413No Hit
TAGTGGGGGCAGCAGACCTATAGTCACGC50.16072002571520413No Hit
TCTCCATAATCCATTAGTGACCAAGCCGCCT50.16072002571520413No Hit
TCTTCACAGGGGCCGTCGGTCTGGGACACT50.16072002571520413No Hit
TATAACGTGGACGTGGTCGTGGTGTTCTG50.16072002571520413No Hit
TGAAAAGCCATGAAGGACCAATTGGTATAGT50.16072002571520413No Hit
TCATATCGCACCCCTATTTCGACACTGCC50.16072002571520413No Hit
TGTGCCGAACCGACGTAAGTTCTGTTGTGG50.16072002571520413No Hit
TTCCTCTTCGCCTTCTTCATAGTTGTCGG50.16072002571520413No Hit
TCCTTCGGTTGACCTACACATTTCGAGTAA50.16072002571520413No Hit
CTTCCTTCGGTTGACCTACACATTTCGAGTA50.16072002571520413No Hit
TACAGTTTGAAGAATTCTAGCACCACAAT50.16072002571520413No Hit
TGTTGTAGATCGTCTGGAACTTGGTAAGAG50.16072002571520413No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTC50.16072002571520413No Hit
TACAAGCTGCTCGTCCCGTACTTTGTAACG50.16072002571520413No Hit
TATTGTTGTAGATCGTCTGGAACTTGGT50.16072002571520413No Hit
TTCGAGCTTCATTACCAAATCGTGGGATCGA50.16072002571520413No Hit
TAATTGTCTCAACAGTTGCCCCGTCTGC50.16072002571520413No Hit
TCCCGAGTTATCGCTTTCTGTAATTGTCTCAAC50.16072002571520413No Hit
TCACAGGGGCCGTCGGTCTGGGACACTCT50.16072002571520413No Hit
TCCTTCGGTTGACCTACACATTTCGAGT50.16072002571520413No Hit
TGGGCACATCTGCACTGGTTGGATTGATAG50.16072002571520413No Hit
TAGATATGGGCACATCTGCACTGGTTGG40.12857602057216327No Hit
TGCTCGATGAAATAGTGGGGGCAGCAGACCT40.12857602057216327No Hit
TTCTCCGGGCACAGTTTCTTCACAGGGGCC40.12857602057216327No Hit
TAATTGGCGGTTTGCTGGTAGATATGGGCACA40.12857602057216327No Hit
TAAACTGCCCATCTCGCCTTTCTTGAAGT40.12857602057216327No Hit
TCGCTTTCTGTAATTGTCTCAACAGTT40.12857602057216327No Hit
TGGTTGGATTGATAGGAACAGTCGTGTAT40.12857602057216327No Hit
TACTTAATTGTGAACTCATGCCGGGGA40.12857602057216327No Hit
CTCGACATCCATAATTGGCGGTTTGCTGGT40.12857602057216327No Hit
CTGTAATTGTCTCAACAGTTGCCCCGTCTGCGCACTGGATG40.12857602057216327No Hit
TTCTGAAATTGTCTCAACAGTTGCCCCGTC40.12857602057216327No Hit
TGAGTACAGTTTGAAGAATTCTAGCACCAC40.12857602057216327No Hit
TGCGCTATTTCGGCCCATAACATGGACAT40.12857602057216327No Hit
TTCGAAGGAGACGGATGTGTGCCGAACC40.12857602057216327No Hit
TACTTGCGCTTGCTGATCGGGAGTCAAAT40.12857602057216327No Hit
TATAACGTGGACGTGGTCGTGGTGTTCTGC40.12857602057216327No Hit
TCTTCGTCCGACTCGACATCCATAATTGGC40.12857602057216327No Hit
TCCGAACGAACTCGAGTAATTGTTCTCCC40.12857602057216327No Hit
TAGGAACAGTCGTGTATAACGTGGACGTG40.12857602057216327No Hit
ACTGCCCATCTCGCCTTTCTTGAAGTCCCGA40.12857602057216327No Hit
TTGCTGGTAGATATGGGCACATCTGCACT40.12857602057216327No Hit
TTTGCTGGTAGATATGGGCACATCTGCAC40.12857602057216327No Hit
TAAGCACACAGTAAGTTTATAAAAAGACTTGAAC40.12857602057216327No Hit
TTAAACTGCCCATCTCGCCTTTCTTGAAG40.12857602057216327No Hit
TTCACAGGGGACGTCGGTCTGGGACACTC40.12857602057216327No Hit
TCCGAACGAACTCGAGTAATTGTTCTCCCGT40.12857602057216327No Hit
TCGTGTATAACGTGGATGTGGTCGTGGT40.12857602057216327No Hit
ATACTTAATTGTGAACTCATGCCGGGGAA40.12857602057216327No Hit
CCCCGTCTGCGCACTGGATGAAACCGT40.12857602057216327No Hit
TTGTGAACTCATGCCGGGGAATAACTCTTC40.12857602057216327No Hit
TCCATAATCCATTAGTGACCAAGCCGCCAAT40.12857602057216327No Hit
TGTAGATCGTCTGGAACTTGGTAAGAGAT40.12857602057216327No Hit
TCGCATGCTGACGCGACCACGCTGCTGC40.12857602057216327No Hit
AGTTTGAAGAATTCTAGCACCACGATGAA40.12857602057216327No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)