FastQCFastQC Report
Mon 30 Nov 2020
S34.raw.31032.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.31032.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143
Sequences flagged as poor quality0
Sequence length12-75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAAGCTGCCCGTCCCGTACTTTGTAACA74.895104895104895No Hit
TACAAGCTGCCCGTCCCGTACTTTGTAACG64.195804195804196No Hit
TCACTTTCGATTTCTTTCCCGTGCTCCGT42.797202797202797No Hit
ACATCGGCCCTCACAATTTAGCCACC32.097902097902098No Hit
AAGCTGCCCGTCCCGTACTTTGTAACG32.097902097902098No Hit
TTTGGCAGTTGGAAATGTAACGTCTGATTTGGACTCA32.097902097902098No Hit
ACGAGCTGCCCGTCCCGTACTTTGTAAC32.097902097902098No Hit
AAGCTGCCCGTCCCGTACTCTGTAACGGA32.097902097902098No Hit
TACCAAGCCGCGCCTTACCACCCTAG32.097902097902098No Hit
ACAAGCTGCCCGTCCCG32.097902097902098No Hit
TCGAACCCGTGACCTTAGGGTTACCAAGCCGCGCCT21.3986013986013985No Hit
TATGCAACTCCTGGATAGTCTTTCCCCCCTCAGGGTA21.3986013986013985No Hit
TCAACAAGCTGCCCGTCCCGTACTTTGTAA21.3986013986013985No Hit
GTTTACAAGCTGCCCGTCCCGTACT21.3986013986013985No Hit
CTAAATAACAAGCTGCCCGTCCCGTACT21.3986013986013985No Hit
CCTTGGGGTTACCAAGCCGCGCCTTACCACCC21.3986013986013985No Hit
TTGGTACTGAAAGGGTTGGCAGTTGGAA21.3986013986013985No Hit
GTTACCAAGCCGCGCCTTACCACACT21.3986013986013985No Hit
TACCAAGCCGCGCCTTACCACCCGA21.3986013986013985No Hit
GGGGTTCGAACCCGTGACCTTAGGGTTACCAAGCCGCGCCTTACCACCC21.3986013986013985No Hit
TAGACATGTATGACCTCCCCTCGGACGACA21.3986013986013985No Hit
TAGACATGTATGACCTCCCCTCGGACGAC21.3986013986013985No Hit
TTGGCAGTTGGAAATGTAACGTCTGATT21.3986013986013985No Hit
TTGGTACTGAAAGGGTTGGCAGTTGGA21.3986013986013985No Hit
TACCAAGCCGCGCCTTACCAC21.3986013986013985No Hit
AAGCTGCCCGTCCCGTACTTTGTAAC21.3986013986013985No Hit
AGCTGCCCGTCCCGTACTTTG21.3986013986013985No Hit
TTGGCAGTTGGAAATGTAACGTCTGATTCGGAC21.3986013986013985No Hit
TTACCAAGCCGCGCCTTACCACCCTAGCT21.3986013986013985No Hit
TACAAGCTGCCCGTCCCGTACTTTGTAACAG21.3986013986013985No Hit
TTGGCAGTTGGAAATGTAACGTCTGATTTGGA21.3986013986013985No Hit
GTTACCAAGCCGCGCCTTACCACCCTAGCTACCTGC21.3986013986013985No Hit
AGCTGCCCGTCCCGTACTTTGTAAC21.3986013986013985No Hit
TCGGAAAAAAACTTGGCGTTTAGGTGTGGTTCACATTCAACATCGGCCCTCACAATTTAGCCACCCTTT10.6993006993006993No Hit
TAACAAGCCGCCCGTCCCGTACTTTG10.6993006993006993No Hit
AAAGTAGCTCGGTGCGGGGTTCGAACCCG10.6993006993006993No Hit
TTACCAAGCCGCGCCTTACCACTCTAGCTAC10.6993006993006993No Hit
TGCCCGTCCCGTACTTTGTAAC10.6993006993006993No Hit
ACCGCGCCTTACCACCC10.6993006993006993No Hit
TTACCAAGCCGCGCCTTACCACCCTAGG10.6993006993006993No Hit
TTGGTACTGAAAGGGATGGCAGTTGGA10.6993006993006993No Hit
NGGGTTCGAACCCGTGACCTTAGGGTTACCAAGCCGCGCCTTACCACCC10.6993006993006993No Hit
CTTCTCACATCTTCTACAGACCCGGTTTGCTCGAATTTTCCACGATTTTTGCGATTGTAAGCACATTTGGACGA10.6993006993006993No Hit
CCAAGCCGCGCCTTACCACCCGA10.6993006993006993No Hit
AAGCTGCCCGTCCCGTACTTTGT10.6993006993006993No Hit
CAAGCTGCCCGTCCCGTACTTTGTAACAGAGA10.6993006993006993No Hit
TACCAAGCCGCGCCTTACCACCCGAG10.6993006993006993No Hit
GCCGCGCCTTACCACCC10.6993006993006993No Hit
CAAGCTGCTCGTCCCGTACTTTGTAA10.6993006993006993No Hit
GTCTTTGATCTCC10.6993006993006993No Hit
AGCTGCCCGTCCCGTACTTTGTAACGCA10.6993006993006993No Hit
TTTTACTTTACTCCCTTGGTACTGAAAGG10.6993006993006993No Hit
TAACGGAGAAAAAAAAGTAGCCCGGTGCGGG10.6993006993006993No Hit
ACCCCTCGGACG10.6993006993006993No Hit
CAAGCTGCCCGTCCCGTACTTTGTAAC10.6993006993006993No Hit
AAAAAAAAATCGGAAAAAAACTTGGCGTTTAGGTGTGGTTCACATTCAACATCGGCCCTCACAATTTAGCCACCC10.6993006993006993No Hit
ATACAAGCTGCCCGTCCCGTA10.6993006993006993No Hit
AACAAGCTGCCCGTCCCGTACT10.6993006993006993No Hit
CAAGCTGCCCGTCCCGTACTTTGTAACGG10.6993006993006993No Hit
ACGAAGTACACGATATGCATTTTGATTTGAACGCCCATTTTCATAATAAGTCTGGATAACTTTAAAACGTTGT10.6993006993006993No Hit
TACAAGCTGCCCGTCCCGTACTTTGTA10.6993006993006993No Hit
TAGGGTTACCAAGCCGCGCCTTACCACCC10.6993006993006993No Hit
TAGCCCGGTGTGGGGTTCGAACCCGTGACC10.6993006993006993No Hit
TACAAGCTGCCCGTCCCGTACTTTGTAA10.6993006993006993No Hit
TTGGCAGTTGGAAATGTAACGTCTGATTCG10.6993006993006993No Hit
TCTTCTACAGACCCGGTTTGCTCGAATTTT10.6993006993006993No Hit
TTACCAAGCCGCGCCTTACCACCCTAGCTA10.6993006993006993No Hit
ATCGTGCACGATATGCATTTTGATTTGAACGCCCATTTTCATAATAAGT10.6993006993006993No Hit
TTCGAACCCGTGACCTTAGGGTTACCAAGCCGCGCCT10.6993006993006993No Hit
TTGGTACTGAAAGGGTTGGCAGTTGG10.6993006993006993No Hit
GACGTCTAGCGTCGGTCCTTGTGTACTCGG10.6993006993006993No Hit
ACAAGCTGCCCGTCCCGTACTTTGTAACGGA10.6993006993006993No Hit
CCGTACTTTGTAA10.6993006993006993No Hit
ACCAAGCCGCGCCTTACCACCCTAGCTACCT10.6993006993006993No Hit
AAATTCGGAAAAAAACTTGGCGTTTAGGTGTGGTTCAC10.6993006993006993No Hit
GCCTCCCCTCGGA10.6993006993006993No Hit
CCGTCCCGTACTTTGTAA10.6993006993006993No Hit
TTGGCAGTTGGAAATGTAACGTCTGATTCGG10.6993006993006993No Hit
TACCAAGCCGCGCCTTACCACCCG10.6993006993006993No Hit
TCGGTCCTTGTGTACTCGGTTTATGAGCAC10.6993006993006993No Hit
GCCGCGCCTTACCACCCT10.6993006993006993No Hit
TTGAACGCCCATTTTCATAATAAGTCTGGATAACTTTAACACGTTGTTGGACTGTGTATCTCTCTATGGTT10.6993006993006993No Hit
AGCCGCGCCTTACCACCC10.6993006993006993No Hit
TAGGCAGTTGGAAATGTAACGTCTGATTCGGA10.6993006993006993No Hit
TTCAACAAGCTGCCCGTCCCGTACTTTGT10.6993006993006993No Hit
CCCGTCCCGTACTTTGTAAC10.6993006993006993No Hit
GGTGCGGGGTTCGAAC10.6993006993006993No Hit
TCAACAAGCTGCCCGTCCCGTACTTTGTAAC10.6993006993006993No Hit
TTCAACAAGCTGCCCGTCCCGTACTTTG10.6993006993006993No Hit
TTACAAGCTGCCCGTCCCGTACT10.6993006993006993No Hit
TATTACAAGCTGCCCGTCCCGTACTTTGT10.6993006993006993No Hit
AAGCCGCGCCTTACCACCCT10.6993006993006993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)