FastQCFastQC Report
Mon 30 Nov 2020
S34.raw.3449.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.3449.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165
Sequences flagged as poor quality0
Sequence length11-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATGATGACA8752.72727272727272No Hit
ATTATGATGAC84.848484848484849No Hit
CATTATGATGACA53.0303030303030303No Hit
GTTAGAACAGGAGGACC42.4242424242424243No Hit
AGTAATGTTAAGGGGGGACGTCACATT42.4242424242424243No Hit
AAGGGCAGTAATGTTAAGGGGGGACGTC31.8181818181818181No Hit
AAGGGCAGTAATGTTAAGGGGGGACGTTC31.8181818181818181No Hit
ATAAAAACGACAGTCAAGGAGAGCACA31.8181818181818181No Hit
TGGACCATGAGAGAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAGGGC31.8181818181818181No Hit
GAATTAAGGAATATTTATTGGATGGTCTACCGGAAGAACTCCTAAGGGCAGTAATGTTAAGGGG21.2121212121212122No Hit
ATGGCCTACCGGAAGAACTCC21.2121212121212122No Hit
AAGGGACTGGCG21.2121212121212122No Hit
AATTAAGGAATATTTATTGGATGGCCT21.2121212121212122No Hit
AAGGGCAGTAATGTTAAGGGGGGACGT21.2121212121212122No Hit
GAATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCT21.2121212121212122No Hit
CCACAAGGGGTGGAG21.2121212121212122No Hit
ATTGGATGGCCTACCGGAAGAACTC21.2121212121212122No Hit
TGGCCTACCGGAAGAACTCCT21.2121212121212122No Hit
TTCATGTACAG10.6060606060606061No Hit
TATTTATTGGATGGCCTACCGGAAGAACTC10.6060606060606061No Hit
GCCTACCGGAAGAACTCC10.6060606060606061No Hit
ATTTATTGGATGGCCTACCGGAAGAACTCCTAAGGGC10.6060606060606061No Hit
GGATGGCCTACCGGAAGAACTCCTAAGG10.6060606060606061No Hit
CTTTGTTTTAAAATAGAACCCGGTATGGACCATGAGAGAATTAAGGAATATTTATTGGATGGCC10.6060606060606061No Hit
ATCCCGCCACC10.6060606060606061No Hit
CCGGAAGAACTCCTAAGGGCAGT10.6060606060606061No Hit
ACCGGAAGAACTCCTAAGGGCAGTAAT10.6060606060606061No Hit
ATAGCGATTATGATG10.6060606060606061No Hit
GCTCCACGTAGACAACACGTCATGTCACCTAGATGATCTACACATCATCT10.6060606060606061No Hit
TAAATATAAAGA10.6060606060606061No Hit
ATAAATACGACAGTCAAGGAGAGCACAAAGTTAGTTAGAACAGGAGGACC10.6060606060606061No Hit
ACGACAGTCAAGGAGAGCACAAAGTTAGTTAGAACAGGAGGACC10.6060606060606061No Hit
CCCACAAGGTTG10.6060606060606061No Hit
GTTCTAACCATAAAC10.6060606060606061No Hit
ACGACAGTCAAGGAGAGCACAAAGTTAGTTAGAACAGGAGGA10.6060606060606061No Hit
TTAGAACAGGAGGACC10.6060606060606061No Hit
ATGAGAAAATAA10.6060606060606061No Hit
CTGGGTTCGACT10.6060606060606061No Hit
ATGATCAACACATCATCTAGTGATAACCACCCTAATCGGTAACGATAAGA10.6060606060606061No Hit
GAACCCGGTATGGACCATGAGAGAATTAAGGAATATTTATTGGATGGCC10.6060606060606061No Hit
AATTAAGGAATATTTATTGGATGGCCTACCGGAAGAACTCCTAAGGGC10.6060606060606061No Hit
AGAGAGAGAAACCTGGATATCCCGCCACCT10.6060606060606061No Hit
AAACGACAGTCAAGGAGAGCACAAAGTTAGTTAGAACAGGAG10.6060606060606061No Hit
CCCTAATCTGTAACGATAAGATGGGAATAAAAACGACAGTCAAGGAGAGC10.6060606060606061No Hit
ACATGTCGAACGC10.6060606060606061No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)