FastQCFastQC Report
Mon 30 Nov 2020
S34.raw.3452.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.3452.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072
Sequences flagged as poor quality0
Sequence length11-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCTCGGTACTGACTCGACAAGCGCC1356.5154440154440145No Hit
TATTGTCGAATAACCCACCTCTTGTTGG1296.225868725868726No Hit
TTGGGCTCGGTACTGACTCGACAAGCGCCT884.247104247104247No Hit
TCTATATTGTCGAATAACCCACCTCTTGG562.7027027027027026No Hit
TGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAA492.364864864864865No Hit
TATTGTCGAATAACCCACCTCTTGG472.2683397683397684No Hit
TTAACGACCCGCTCTGTTCCATATCGATA462.2200772200772203No Hit
TATACGTCGTGTACGACGCGAGACCGCGGA391.8822393822393824No Hit
TAAAAGGACCGATTCGGGCAGTGAGTGTC371.7857142857142856No Hit
TTAACGACCCGCTCTGTTCCATATCGATAT331.5926640926640927No Hit
TTTGTTAACTGGCCGACTTCCCCCGCCT311.4961389961389961No Hit
TGAATTGCCGTGGCTCGGAAGTTGGGCTC281.3513513513513513No Hit
TCGGTACTGACTCGACAAGCGCCTCGTAC271.303088803088803No Hit
TCGGTACTGACTCGACAAGCGCCTCGTACT271.303088803088803No Hit
CACGTCGTGTACGACGCGAGACCGCGGAG261.2548262548262548No Hit
TGGGCTCGGTACTGACTCGACAAGCGCCT251.2065637065637065No Hit
TAACGACCCGCTCTGTTCCATATCGATAT241.1583011583011582No Hit
TAACGACCCGCTCTGTTCCATATCGATATT200.9652509652509652No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGAC190.916988416988417No Hit
AAAAGTCACCGTCGTCCTCATGACTGCCT190.916988416988417No Hit
TATTGTCGAATAACCCACCTCTTGGT190.916988416988417No Hit
AAGTGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAA140.6756756756756757No Hit
TGGGCTCGGTACTGACTCGACAAGCGCC140.6756756756756757No Hit
TGAATTGCGGTGGCTCGGAAGTTGGGCTC140.6756756756756757No Hit
TAACACAAGCCCGATCGCTCCGATACATG140.6756756756756757No Hit
TGGGGATCCCGTAAGAAATACACGTCGTGT130.6274131274131274No Hit
TTGGGGATCCCGTAAGAAATACACGTCGTG130.6274131274131274No Hit
TCCCTCTTCGAACGTAACACAAGCCCGATC120.5791505791505791No Hit
TTAGATGGACCCGGTATAGGATTTTCGCTC120.5791505791505791No Hit
TGAAAGTGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAA120.5791505791505791No Hit
TATATTGTCGAATAACCCACCTCTTGG110.5308880308880308No Hit
TAACGACCCGCTCTGTTCCATATCGATA100.4826254826254826No Hit
TCCCAAACTTCGACTCGGACCAGTGCCGG100.4826254826254826No Hit
CATTGTCGAATAACCCACCTCTTGG90.4343629343629344No Hit
TGCCGAAGCCATAAAAGGACCGATTCGGGC90.4343629343629344No Hit
TTCGACTGTTAGGTGGGCAAAATTTGGGA90.4343629343629344No Hit
TCGTGTACGACGCGAGACCGCGGAGGCA80.3861003861003861No Hit
TTCTTGTTCGGAAAAGTCGCTGGCTTCGT80.3861003861003861No Hit
CATTGTCGAATAACCCACCTCTTGGT80.3861003861003861No Hit
TATAGACGTTAACGGCGCTGAGGGGTGT80.3861003861003861No Hit
TCTATATTGTCGAATAACCCACCTCTTGGT80.3861003861003861No Hit
TTAGATGGACCCGGAATAGGATTTTCGCTC70.33783783783783783No Hit
TCAAAACTCCCTCTTCGAACGTAACACAAGC70.33783783783783783No Hit
TACTTGGATTTGTGGGATGATGGCATCGC70.33783783783783783No Hit
CACGTCGTGTACGACGCGAGACCGCGGAGG70.33783783783783783No Hit
TGAATTGCCGTGGCTCGGAAGTTGGGCTCG70.33783783783783783No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGCC70.33783783783783783No Hit
TGTCTTTTGTTAACTGGCCGACTTCCCCC70.33783783783783783No Hit
TTATTAATGAGACCGCGGAGGACTATCTC70.33783783783783783No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGCC60.28957528957528955No Hit
TGACTGCCTCCTGCCACCAATCCTTCTTGT60.28957528957528955No Hit
TACAATTTCTGCATCGTTATAGCACTGAT60.28957528957528955No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGC60.28957528957528955No Hit
CATGCCGAACTC60.28957528957528955No Hit
TCCGATACATGGATTGAACTTCCTGAGTGT60.28957528957528955No Hit
TCATCTTCTTGTTCGGAAAAGTCGCTGGCT60.28957528957528955No Hit
TTCGAAGGGTTTCGAGAAATTGGGACACT60.28957528957528955No Hit
TGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCA60.28957528957528955No Hit
GTGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAA60.28957528957528955No Hit
TAGATGGACCCGGAATAGGATTTTCGCTC60.28957528957528955No Hit
TATTGTCGAATAACCCACCTCTTGGTG50.2413127413127413No Hit
TGAGGACCAGGGGCTGTGTGAGGGCCTAA50.2413127413127413No Hit
TTAACGACCCGCTCTGTTCCATATCGA50.2413127413127413No Hit
TTGGGCTCGGTACTGACTCGACAAGCG50.2413127413127413No Hit
TAAAAGGACCGATTCGGGCAGTGAGTGTCTT50.2413127413127413No Hit
CTTGGATCGGAAATGTTCATGGGGACGTT50.2413127413127413No Hit
TATTCACTGGCTTCCGCTTCAAAACTCCT50.2413127413127413No Hit
TTGGGGATCCCGTAAGAAATACACGTCGT50.2413127413127413No Hit
TTAACGACCCGCTCTGTTCCATATCGAT50.2413127413127413No Hit
TGGGGATCCCGTAAGAAATACACGTCGTG50.2413127413127413No Hit
TGCAATATAGACGTTAACGGCGCTGAGGGG50.2413127413127413No Hit
AACACAAGCCCGATCGCTCCGATACATGG50.2413127413127413No Hit
TCCTGAGTGTCCGGAAGATCCTTCTGACG50.2413127413127413No Hit
GGAGTACGGCC50.2413127413127413No Hit
TTTGAACGTACATAACTTTTCTCCGACCCT50.2413127413127413No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGACT50.2413127413127413No Hit
TCGGATCCTATTGCATATGCGGATGCATC50.2413127413127413No Hit
TCTGAGAAAAAGTCACCGTCGTCCTCATG50.2413127413127413No Hit
TAGACAGAATAATAACAGGAGTACGGCCCAC50.2413127413127413No Hit
TCTTCTTGTTCGGAAAAGTCGCTGGCTTCG40.19305019305019305No Hit
TTGGATTTGTGGGATGATGGCATCGCCATTTT40.19305019305019305No Hit
TACACGTCGTGTACGACGCGAGACCGCGGA40.19305019305019305No Hit
TGGCTTCGTATTCACTGGCTTCCGCTTCA40.19305019305019305No Hit
TCGACTGTTAGGTGGGCAAAATTTGGGA40.19305019305019305No Hit
TTTGTCCGGAG40.19305019305019305No Hit
TATACGTCGTGTACGACGCGAGACCGCGGAT40.19305019305019305No Hit
TGAGGACCAGGGGCTGTGTGAGGGCCTA40.19305019305019305No Hit
TACTGACTCGACAAGCGCCTCGTACTTGT40.19305019305019305No Hit
GGATGAAGACGCAGCGGCATGTTGAACTG40.19305019305019305No Hit
GATGTTGGGAAGGGGATACCTATCGCCT40.19305019305019305No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGC40.19305019305019305No Hit
GTTTGTCCGGAG40.19305019305019305No Hit
CACAAGCCCGATCGCTCCGATACATGGATT40.19305019305019305No Hit
TCTACATTGTCGAATAACCCACCTCTTGGT40.19305019305019305No Hit
TTGGAGGGCGTCCTGTATGCGCCGGATGAAGACGCAGCGGCATGTTG40.19305019305019305No Hit
TAGGGAATTTTTGAATGGCTGCTATTTT40.19305019305019305No Hit
TTCTACTTGGATTTGTGGGATGATGGCATC40.19305019305019305No Hit
TGCAATATAGACGTTAACGGCGCTGAGGG30.14478764478764478No Hit
ACCGCGGAGGCACTCTTTGAAGCAGT30.14478764478764478No Hit
TTAGACAGAATAATAACAGGAGTACGGCCC30.14478764478764478No Hit
TGTCGATTAACGAGCTCCACACTTCTTTGG30.14478764478764478No Hit
TGTTAACTGGCCGACTTCCCCCGCCT30.14478764478764478No Hit
TTTTTTTGGGCGGATTCTTTCGGGGGT30.14478764478764478No Hit
TCGGTACTGACTCGACAAGCGCCTCGTA30.14478764478764478No Hit
TAGACGTTAACGGCGCTGAGGGGTGTTG30.14478764478764478No Hit
TCTTCTTGTTCGGAAAAGTCGCTGGCTTC30.14478764478764478No Hit
CTTCTTGTTCGGAAAAGTCGC30.14478764478764478No Hit
CGCGAGACCGCGGAGGCACTCTTTGAAGG30.14478764478764478No Hit
TGGCAGGAGATTGAACGTTTGTTTCGGTG30.14478764478764478No Hit
CACAGACGCGGTGTTTACTCTCTG30.14478764478764478No Hit
GACCGCGGAGGCACTCTTTGAAGG30.14478764478764478No Hit
TCAAAACTCCCTCTTCGAACGTAACACAAG30.14478764478764478No Hit
CACAAGCCCGATCGCTCCGATACATGGAT30.14478764478764478No Hit
TCACACACACTACTGATACTATCGGTCTCA30.14478764478764478No Hit
TGGCCGACTTCCCCCGCCTGAAAGTGGTCCATTAAGGCCTTGTTGTAGAC30.14478764478764478No Hit
TATTGTCGAATAACCCACCTCTTGTTG30.14478764478764478No Hit
TTCGAAGGGTTTCGAGAAATTGGGACA30.14478764478764478No Hit
CCGTCGTCCTCATGACTGCCTCCTGCCACC30.14478764478764478No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGCCT30.14478764478764478No Hit
TGCCACGACGCATTTTCGTAGTTCTCCCCA30.14478764478764478No Hit
TGTACAATTTCTGCATCGTTATAGCACTGAT30.14478764478764478No Hit
ATGGCAGGAGATTGAACGTTTGTTTCGGTGTCCACACGAGAAACAGAAACGCGAGA30.14478764478764478No Hit
TACTGACTCGACAAGCGCCTCGTACTTG30.14478764478764478No Hit
TTCGAGAAATTGGGACACTGTAATATTGGG30.14478764478764478No Hit
TGCAGTCGTGGATGACGTGGACGTGGTCGT30.14478764478764478No Hit
TTCCAACGTCTCGTTCCATCGGATCACTT30.14478764478764478No Hit
TCTTGGATCGGAAATGTTCATGGGGACGTT30.14478764478764478No Hit
TGGCCGACTTCCCCCGCCTGAAAGTGGTC30.14478764478764478No Hit
TCTTGTGAATTCGACTGTTAGGTGGGCA30.14478764478764478No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGA30.14478764478764478No Hit
TGTCTTTTGTTAACTGGCCGACTTCCCCT30.14478764478764478No Hit
TTGGATCGGAAATGTTCATGGGGACGTT30.14478764478764478No Hit
TCTGAGTATGGGGCTATTTCCGTGTGGAAA30.14478764478764478No Hit
TACAACTCTGCCATAGACACGTCCATTAGT30.14478764478764478No Hit
TCACAATTTCCAACGTCTCGTTCCATCGGA30.14478764478764478No Hit
TCAGTGTTGAAGGCTGTTTTTGGAATTGA30.14478764478764478No Hit
CACATGAGTTGAATTGCCGTGGCTCGGAA30.14478764478764478No Hit
TCGTGGATGACGTGGACGTGGTCGTTGTG30.14478764478764478No Hit
TTTTGTTAACTGGCCGACTTCCCCCGCCT30.14478764478764478No Hit
TGGATCGGAAATATTCATGGGGACGTTT30.14478764478764478No Hit
TCCTCATGACTGCCTCCTGCCACCAATCC30.14478764478764478No Hit
TGCATGAAGGAAGATTGATGGCAGGAGAT30.14478764478764478No Hit
TTGTACAATTTCTGCATCGTTATAGCACT30.14478764478764478No Hit
CTGGTGTAACTGCAGTCGTGGATGACGTGGACGTGGTCGTTGTGGAGGCA30.14478764478764478No Hit
TACACGTCGTGTACGACGCGAGACCGCGGAGGCA30.14478764478764478No Hit
CACGACGCATTTTCGTAGTTCTCCCCAAGC30.14478764478764478No Hit
TGCCGAACTCTGAGAAAAAGTCACCGTCGTC30.14478764478764478No Hit
TGTCTTTTGTTAACTGGCCGACTTCCCCCG30.14478764478764478No Hit
CACGTCGTGTACGACGCGAGACCG30.14478764478764478No Hit
TGTGTCAGTGTTACTAATGATTCCTTCTTC30.14478764478764478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)