FastQCFastQC Report
Mon 30 Nov 2020
S34.raw.4554.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.4554.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39
Sequences flagged as poor quality0
Sequence length11-21
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACTTGAAT1846.15384615384615No Hit
AGGACTTGAAT1128.205128205128204No Hit
GGGAAGTCGGC410.256410256410255No Hit
CTCCGCGGTCT25.128205128205128No Hit
TAACCCGGCACT12.564102564102564No Hit
CTTGCAAACCCGGCACTGGTC12.564102564102564No Hit
AGAAACTAACG12.564102564102564No Hit
AGGGAAGTCGGC12.564102564102564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)