FastQCFastQC Report
Mon 30 Nov 2020
S34.raw.4554.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.4554.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930
Sequences flagged as poor quality0
Sequence length11-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCTCGGTACTGACTCGACAAGCGCC1356.994818652849741No Hit
TATTGTCGAATAACCCACCTCTTGTTGG1296.683937823834196No Hit
TTGGGCTCGGTACTGACTCGACAAGCGCCT884.5595854922279795No Hit
TCTATATTGTCGAATAACCCACCTCTTGG562.901554404145078No Hit
TGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAA492.5388601036269427No Hit
TATTGTCGAATAACCCACCTCTTGG472.4352331606217614No Hit
TTAACGACCCGCTCTGTTCCATATCGATA462.383419689119171No Hit
TATACGTCGTGTACGACGCGAGACCGCGGA392.0207253886010363No Hit
TAAAAGGACCGATTCGGGCAGTGAGTGTC371.917098445595855No Hit
TTAACGACCCGCTCTGTTCCATATCGATAT331.709844559585492No Hit
TTTGTTAACTGGCCGACTTCCCCCGCCT311.6062176165803108No Hit
TGAATTGCCGTGGCTCGGAAGTTGGGCTC281.450777202072539No Hit
TCGGTACTGACTCGACAAGCGCCTCGTAC271.3989637305699483No Hit
TCGGTACTGACTCGACAAGCGCCTCGTACT271.3989637305699483No Hit
CACGTCGTGTACGACGCGAGACCGCGGAG261.3471502590673576No Hit
TGGGCTCGGTACTGACTCGACAAGCGCCT251.2953367875647668No Hit
TAACGACCCGCTCTGTTCCATATCGATAT241.2435233160621761No Hit
TAACGACCCGCTCTGTTCCATATCGATATT201.0362694300518136No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGAC190.9844559585492229No Hit
AAAAGTCACCGTCGTCCTCATGACTGCCT190.9844559585492229No Hit
TATTGTCGAATAACCCACCTCTTGGT190.9844559585492229No Hit
AAGTGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAA140.7253886010362695No Hit
TGGGCTCGGTACTGACTCGACAAGCGCC140.7253886010362695No Hit
TGAATTGCGGTGGCTCGGAAGTTGGGCTC140.7253886010362695No Hit
TAACACAAGCCCGATCGCTCCGATACATG140.7253886010362695No Hit
TGGGGATCCCGTAAGAAATACACGTCGTGT130.6735751295336788No Hit
TTGGGGATCCCGTAAGAAATACACGTCGTG130.6735751295336788No Hit
TCCCTCTTCGAACGTAACACAAGCCCGATC120.6217616580310881No Hit
TGAAAGTGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAA120.6217616580310881No Hit
TATATTGTCGAATAACCCACCTCTTGG110.5699481865284974No Hit
TAACGACCCGCTCTGTTCCATATCGATA100.5181347150259068No Hit
TCCCAAACTTCGACTCGGACCAGTGCCGG100.5181347150259068No Hit
CATTGTCGAATAACCCACCTCTTGG90.466321243523316No Hit
TGCCGAAGCCATAAAAGGACCGATTCGGGC90.466321243523316No Hit
TTCGACTGTTAGGTGGGCAAAATTTGGGA90.466321243523316No Hit
TCGTGTACGACGCGAGACCGCGGAGGCA80.41450777202072536No Hit
CATTGTCGAATAACCCACCTCTTGGT80.41450777202072536No Hit
TATAGACGTTAACGGCGCTGAGGGGTGT80.41450777202072536No Hit
TCTATATTGTCGAATAACCCACCTCTTGGT80.41450777202072536No Hit
TCAAAACTCCCTCTTCGAACGTAACACAAGC70.3626943005181347No Hit
CACGTCGTGTACGACGCGAGACCGCGGAGG70.3626943005181347No Hit
TGAATTGCCGTGGCTCGGAAGTTGGGCTCG70.3626943005181347No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGCC70.3626943005181347No Hit
TGTCTTTTGTTAACTGGCCGACTTCCCCC70.3626943005181347No Hit
TTATTAATGAGACCGCGGAGGACTATCTC70.3626943005181347No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGCC60.31088082901554404No Hit
TGACTGCCTCCTGCCACCAATCCTTCTTGT60.31088082901554404No Hit
TACAATTTCTGCATCGTTATAGCACTGAT60.31088082901554404No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGC60.31088082901554404No Hit
CATGCCGAACTC60.31088082901554404No Hit
TCCGATACATGGATTGAACTTCCTGAGTGT60.31088082901554404No Hit
TTCGAAGGGTTTCGAGAAATTGGGACACT60.31088082901554404No Hit
TGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCA60.31088082901554404No Hit
GTGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAA60.31088082901554404No Hit
TATTGTCGAATAACCCACCTCTTGGTG50.2590673575129534No Hit
TGAGGACCAGGGGCTGTGTGAGGGCCTAA50.2590673575129534No Hit
TTAACGACCCGCTCTGTTCCATATCGA50.2590673575129534No Hit
TTGGGCTCGGTACTGACTCGACAAGCG50.2590673575129534No Hit
TAAAAGGACCGATTCGGGCAGTGAGTGTCTT50.2590673575129534No Hit
CTTGGATCGGAAATGTTCATGGGGACGTT50.2590673575129534No Hit
TTGGGGATCCCGTAAGAAATACACGTCGT50.2590673575129534No Hit
TTAACGACCCGCTCTGTTCCATATCGAT50.2590673575129534No Hit
TGGGGATCCCGTAAGAAATACACGTCGTG50.2590673575129534No Hit
TGCAATATAGACGTTAACGGCGCTGAGGGG50.2590673575129534No Hit
AACACAAGCCCGATCGCTCCGATACATGG50.2590673575129534No Hit
TCCTGAGTGTCCGGAAGATCCTTCTGACG50.2590673575129534No Hit
GGAGTACGGCC50.2590673575129534No Hit
TTTGAACGTACATAACTTTTCTCCGACCCT50.2590673575129534No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGACT50.2590673575129534No Hit
TCGGATCCTATTGCATATGCGGATGCATC50.2590673575129534No Hit
TCTGAGAAAAAGTCACCGTCGTCCTCATG50.2590673575129534No Hit
TAGACAGAATAATAACAGGAGTACGGCCCAC50.2590673575129534No Hit
TACACGTCGTGTACGACGCGAGACCGCGGA40.20725388601036268No Hit
TCGACTGTTAGGTGGGCAAAATTTGGGA40.20725388601036268No Hit
TTTGTCCGGAG40.20725388601036268No Hit
TATACGTCGTGTACGACGCGAGACCGCGGAT40.20725388601036268No Hit
TGAGGACCAGGGGCTGTGTGAGGGCCTA40.20725388601036268No Hit
TACTGACTCGACAAGCGCCTCGTACTTGT40.20725388601036268No Hit
GGATGAAGACGCAGCGGCATGTTGAACTG40.20725388601036268No Hit
GATGTTGGGAAGGGGATACCTATCGCCT40.20725388601036268No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGC40.20725388601036268No Hit
GTTTGTCCGGAG40.20725388601036268No Hit
CACAAGCCCGATCGCTCCGATACATGGATT40.20725388601036268No Hit
TCTACATTGTCGAATAACCCACCTCTTGGT40.20725388601036268No Hit
TTGGAGGGCGTCCTGTATGCGCCGGATGAAGACGCAGCGGCATGTTG40.20725388601036268No Hit
TAGGGAATTTTTGAATGGCTGCTATTTT40.20725388601036268No Hit
TGCAATATAGACGTTAACGGCGCTGAGGG30.15544041450777202No Hit
TTGTTCACTTTCATCTTCTCCTCTCGAT30.15544041450777202No Hit
ACCGCGGAGGCACTCTTTGAAGCAGT30.15544041450777202No Hit
TTAGACAGAATAATAACAGGAGTACGGCCC30.15544041450777202No Hit
TGTCGATTAACGAGCTCCACACTTCTTTGG30.15544041450777202No Hit
TGTTAACTGGCCGACTTCCCCCGCCT30.15544041450777202No Hit
TTTTTTTGGGCGGATTCTTTCGGGGGT30.15544041450777202No Hit
TCGGTACTGACTCGACAAGCGCCTCGTA30.15544041450777202No Hit
TAGACGTTAACGGCGCTGAGGGGTGTTG30.15544041450777202No Hit
CGCGAGACCGCGGAGGCACTCTTTGAAGG30.15544041450777202No Hit
TGGCAGGAGATTGAACGTTTGTTTCGGTG30.15544041450777202No Hit
GACCGCGGAGGCACTCTTTGAAGG30.15544041450777202No Hit
TCAAAACTCCCTCTTCGAACGTAACACAAG30.15544041450777202No Hit
CACAAGCCCGATCGCTCCGATACATGGAT30.15544041450777202No Hit
TGGCCGACTTCCCCCGCCTGAAAGTGGTCCATTAAGGCCTTGTTGTAGAC30.15544041450777202No Hit
TATTGTCGAATAACCCACCTCTTGTTG30.15544041450777202No Hit
TTCGAAGGGTTTCGAGAAATTGGGACA30.15544041450777202No Hit
CCGTCGTCCTCATGACTGCCTCCTGCCACC30.15544041450777202No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGCCT30.15544041450777202No Hit
TGCCACGACGCATTTTCGTAGTTCTCCCCA30.15544041450777202No Hit
TGTACAATTTCTGCATCGTTATAGCACTGAT30.15544041450777202No Hit
ATGGCAGGAGATTGAACGTTTGTTTCGGTGTCCACACGAGAAACAGAAACGCGAGA30.15544041450777202No Hit
TACTGACTCGACAAGCGCCTCGTACTTG30.15544041450777202No Hit
TTCGAGAAATTGGGACACTGTAATATTGGG30.15544041450777202No Hit
TGCAGTCGTGGATGACGTGGACGTGGTCGT30.15544041450777202No Hit
TTCCAACGTCTCGTTCCATCGGATCACTT30.15544041450777202No Hit
TCTTGGATCGGAAATGTTCATGGGGACGTT30.15544041450777202No Hit
TGGCCGACTTCCCCCGCCTGAAAGTGGTC30.15544041450777202No Hit
TCTTGTGAATTCGACTGTTAGGTGGGCA30.15544041450777202No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGA30.15544041450777202No Hit
TGTCTTTTGTTAACTGGCCGACTTCCCCT30.15544041450777202No Hit
TTGGATCGGAAATGTTCATGGGGACGTT30.15544041450777202No Hit
TACAACTCTGCCATAGACACGTCCATTAGT30.15544041450777202No Hit
TCACAATTTCCAACGTCTCGTTCCATCGGA30.15544041450777202No Hit
TCAGTGTTGAAGGCTGTTTTTGGAATTGA30.15544041450777202No Hit
CACATGAGTTGAATTGCCGTGGCTCGGAA30.15544041450777202No Hit
TCGTGGATGACGTGGACGTGGTCGTTGTG30.15544041450777202No Hit
TTTTGTTAACTGGCCGACTTCCCCCGCCT30.15544041450777202No Hit
TGGATCGGAAATATTCATGGGGACGTTT30.15544041450777202No Hit
TCCTCATGACTGCCTCCTGCCACCAATCC30.15544041450777202No Hit
TGCATGAAGGAAGATTGATGGCAGGAGAT30.15544041450777202No Hit
TTGTACAATTTCTGCATCGTTATAGCACT30.15544041450777202No Hit
CTGGTGTAACTGCAGTCGTGGATGACGTGGACGTGGTCGTTGTGGAGGCA30.15544041450777202No Hit
TACACGTCGTGTACGACGCGAGACCGCGGAGGCA30.15544041450777202No Hit
CACGACGCATTTTCGTAGTTCTCCCCAAGC30.15544041450777202No Hit
TGCCGAACTCTGAGAAAAAGTCACCGTCGTC30.15544041450777202No Hit
TGTCTTTTGTTAACTGGCCGACTTCCCCCG30.15544041450777202No Hit
CACGTCGTGTACGACGCGAGACCG30.15544041450777202No Hit
TGTGTCAGTGTTACTAATGATTCCTTCTTC30.15544041450777202No Hit
GACCGCGGAGGCACTCTTTGAAGCAGT20.10362694300518134No Hit
TAACACAAGCCCGATCGCTCCGATACATGGATTGAACTTCCTGAG20.10362694300518134No Hit
TCGATTAACGAACTCCACACTTCTTTGGG20.10362694300518134No Hit
TCGTACTTGTCGATTAACGAGCTCCACACT20.10362694300518134No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGCCT20.10362694300518134No Hit
CCATTGGAGGGC20.10362694300518134No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGCCC20.10362694300518134No Hit
TTTACGGTAGTAGCCTAGTAATCCGAGAAA20.10362694300518134No Hit
TTCGAGAAATTGGGACACTGTAATATTGG20.10362694300518134No Hit
TTTACGGTAGTAGCCTAGTAATCCGAGAA20.10362694300518134No Hit
TCTTGCTTTCGACGCGCTAGAGTGGCTGC20.10362694300518134No Hit
TGCGCCGGATGAAGACGCAGCGGCATGTTG20.10362694300518134No Hit
TACTGACTCGACAAGCGCCTCGTACT20.10362694300518134No Hit
TTGGGCGGATTCTTTCGGGGGTGACA20.10362694300518134No Hit
CAATATAGACGTTAACGGCGCTGAGGGG20.10362694300518134No Hit
TCATGACTGCCTCCTGCCACCAATCCT20.10362694300518134No Hit
TGAACGTTTGTTTCGGTGTCCACACGAGAA20.10362694300518134No Hit
TAACAGGAGTACGGCCCACAGTAGCGTCGCCT20.10362694300518134No Hit
CTTGTTGTAGACCTCTGTCGCACGGCCC20.10362694300518134No Hit
CATTGGAGGGC20.10362694300518134No Hit
TATAGACGTTAACGGCGCTGAGGGGTGTT20.10362694300518134No Hit
TTAACGACCCGCTCTGTTCCATATCGATATT20.10362694300518134No Hit
TGGGGATCCCGTAAGAAATACAC20.10362694300518134No Hit
TAACAGGAGTACGGCCCACAGTAGCGTCGCT20.10362694300518134No Hit
TAGACGTTAACGGCGCTGAGGGGTGT20.10362694300518134No Hit
TATTGTCGAATAACCCACCTCTTGTT20.10362694300518134No Hit
TATACGTCGTGTACGACGCGAGACCGCGGAC20.10362694300518134No Hit
TAACAGGAGTACGGCCCACAGTAGCGTCGCC20.10362694300518134No Hit
AACGACCCGCTCTGTTCCATATCGATATTGT20.10362694300518134No Hit
TCATGACTGCCTCCTGCCACCAATCCTTC20.10362694300518134No Hit
AAAAGGACCGA20.10362694300518134No Hit
CTTGCGGCTCATTCCAAAGAAGAATTCTT20.10362694300518134No Hit
ACGTGGTCGTTGTGGAGGCACATGAGTTT20.10362694300518134No Hit
TCCAGACTTCTTTGGGGATCCCGTAAGAAA20.10362694300518134No Hit
CATTGTCGAATAACCCACCTCTTG20.10362694300518134No Hit
TTGGGCGGATTCTTTCGGGGGTGACAATAT20.10362694300518134No Hit
TGCATGAAGGAAGATTGATGGCAGGAGATT20.10362694300518134No Hit
TGCATCGGTAGTTAATTCGAAGGGTTTCGA20.10362694300518134No Hit
TTCGAACGTAACACAAGCCCGATCGCTCCGATA20.10362694300518134No Hit
TGCTTTCGACGCGCTAGAGTGGCTGCAT20.10362694300518134No Hit
TACATGGATTGAACTTCCTGAGTGTCCGGAA20.10362694300518134No Hit
TTAACGACCCGCTCTGTTCCATATCGATC20.10362694300518134No Hit
TTGAATTGCCGTGGCTCGGAAGTTGGGC20.10362694300518134No Hit
CTGGCCGACTTCCCCCGCCTGAAAGTGGTC20.10362694300518134No Hit
TCTGCCATAGACACGTCCATTAGTTCAAGC20.10362694300518134No Hit
ACGACCCGCTCTGTTCCATATCGATATTGTC20.10362694300518134No Hit
CACGTCGTGTACGACGCGAGACCGCGGA20.10362694300518134No Hit
TTAGTCCCAAACTTCGACTCGGACCAGTGCCGGGTTT20.10362694300518134No Hit
TTATTAATGAGACCGCGGAGGACTATC20.10362694300518134No Hit
GATGCGAGACCGCGGAGGCACTCTTTGAAG20.10362694300518134No Hit
TCGAAGGGTTTCGAGAAATTGGGACACT20.10362694300518134No Hit
TAACACAAGCCCGATCGCTCCGATACATGG20.10362694300518134No Hit
TCCTTCTGACGAATTCGAATCTGCGTTTC20.10362694300518134No Hit
TACACGTCGTGTACGACGCGAGACCGCGGAGGCACTCTTTGAAGGAG20.10362694300518134No Hit
TAACAGGAGTACGGCCCACAGTAGCGTCGC20.10362694300518134No Hit
TGTTGGTTCTGCTTGCGGCTCATTCCAAA20.10362694300518134No Hit
TCGCAAGTGTTTAAGATGTTCTTCTAGGG20.10362694300518134No Hit
TCTGCATCGTTATAGCACTGATAGAATAGT20.10362694300518134No Hit
TTGAATTGCCGTGGCTCGGAAGTTGGGCTC20.10362694300518134No Hit
TGATTTCATCTGTGTTTGTGTTCGTGG20.10362694300518134No Hit
CCCTCTTCGAACGTAACACAAGCCCGATCGC20.10362694300518134No Hit
ATTTTCGTAGTTCTCCCCAAGTTTTTGTTT20.10362694300518134No Hit
TAACTGCAGTCGTGGATGACGTGGACGT20.10362694300518134No Hit
TCGTGGATGACGTGGACGTGGTCGTTGT20.10362694300518134No Hit
TTGTTCACTTTCATCTTCTCCTCTCGATTT20.10362694300518134No Hit
TCGTGGAATAGGGAATTTTTGAATGGCTGCTATTTTTTTTGGGCGGATT20.10362694300518134No Hit
TCTGCATCGTTATAGCACTGATAGAATAG20.10362694300518134No Hit
TTGTCGAATAACCCACCTCTTGTTG20.10362694300518134No Hit
TGGTGTAACTGCAGTCGTGGATGACGTGG20.10362694300518134No Hit
TAGGGAATTTTTGAATGGCTGCTATTT20.10362694300518134No Hit
TCGCCAGGTACGCCCTCCGATTTGAACGTAC20.10362694300518134No Hit
CTTGTTGTAGACCTCTGTCGCACGGCCCAATGCCGAACTCTGAGAAAAAG20.10362694300518134No Hit
TGGGCTCGGTACTGACTCGACAAGCG20.10362694300518134No Hit
TATGCGCCGGATGAAGACGCAGCGGCATG20.10362694300518134No Hit
TCCCGTAAGAAATACACGTCGTGTACGACGCGAGACCGCGGAGGCACTCT20.10362694300518134No Hit
TCGTTCCATCGGATCACTTGGCGTTTGAGT20.10362694300518134No Hit
TTAGTCCCAAACTTCGACTCGGACCAGTGCC20.10362694300518134No Hit
TCGTACAACTCTGCCATAGACACGTCCATT20.10362694300518134No Hit
TAGAGTGGCTGCATGAAGGAAGGTT20.10362694300518134No Hit
TCAATCTTTTTGTGAATTCGACTGTTAGGT20.10362694300518134No Hit
GTGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAAT20.10362694300518134No Hit
TGTATCTTGGATCGGAAATGTTCATGGGG20.10362694300518134No Hit
TAACAGGAGTACGGCCCACAGTAGCGTC20.10362694300518134No Hit
CCCCCGCCTGAAAGTGGTCCATTAAGGCCT20.10362694300518134No Hit
TTTGTGAATTCGACTGTTAGGTGGGCAA20.10362694300518134No Hit
TCGGTACTGACTCGACAAGCGCCTCGTACTT20.10362694300518134No Hit
TGCCTCCTGCCACCAATCCTTCTTGTTT20.10362694300518134No Hit
TCCTCATGACTGCCTCCTGCCACCAATCCT20.10362694300518134No Hit
TAACGACCCGCTCTGTTCC20.10362694300518134No Hit
TCCTCTCGATTTGCGGGGAGGAGGACT20.10362694300518134No Hit
AGACCTCTGTCGCACGGCCCAATGCCGAACTCTGAGAAAAAGTCACCGTCGTCCTCATGACTGCCTCCTGCCACC20.10362694300518134No Hit
CCGCGGAGGCACTCTTTGAAGCAGTGCCA20.10362694300518134No Hit
TGGACGTGGTCGTTGTGGAGGCACATGAGT20.10362694300518134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)