FastQCFastQC Report
Mon 30 Nov 2020
S34.raw.5721.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.5721.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167
Sequences flagged as poor quality0
Sequence length11-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCAGAATTGACGGCCCACCAAACATC105.9880239520958085No Hit
ACTGACGTGGAAGTGTGCCAGCTCAAGCAGGAAATAGAGTTGCTGCGTAACGAGC63.592814371257485No Hit
TACAAACGATCTCCTGAGCGCTGGAACTG63.592814371257485No Hit
GCAGTTGCTGCG63.592814371257485No Hit
TGCAGCGGGCAGAGGGAGAGCT42.3952095808383236No Hit
GCTGCAGCGGGCAGAGGGAGAGCTGGAGGAC42.3952095808383236No Hit
TCACTTTCGATTTCTTTCCCGTGCTCCGT42.3952095808383236No Hit
TGCAGCGGGCAGAGGGAGAGCTGGAGGACAGATGTTGGACCC42.3952095808383236No Hit
TGAAGAAATTACAAACGATCTCCTGAGCGC31.7964071856287425No Hit
ACATCGGCCCTCACAATTTAGCCACC31.7964071856287425No Hit
TTTGGCAGTTGGAAATGTAACGTCTGATTTGGACTCA31.7964071856287425No Hit
TAAATTGCAGAATTGACGGCCCACCAAAC31.7964071856287425No Hit
TTAAATTGCAGAATTGACGGCCCACCAAAC31.7964071856287425No Hit
TCTCCTGAGCGCTGGAACTGAACCATGTA31.7964071856287425No Hit
CAACCTGCAGAAGGAGCTGGAACGCGCC31.7964071856287425No Hit
TTTAAATTGCAGAATTGACGGCCCACCAAAC31.7964071856287425No Hit
TATGACGGCCCACCAAACATCAAAGAGAA31.7964071856287425No Hit
TATGCAACTCCTGGATAGTCTTTCCCCCCTCAGGGTA21.1976047904191618No Hit
TGCAGAATTGACGGCCCACCAAACATC21.1976047904191618No Hit
GCAGAGGGAGAGCTGGAGGACAGA21.1976047904191618No Hit
TTGGTACTGAAAGGGTTGGCAGTTGGAA21.1976047904191618No Hit
TATGACGGCCCACCAAACATCAAAGAGA21.1976047904191618No Hit
TGAAATTTTGGCCGGTAGCGCTGCGCCGGACGGACGCGCGCC21.1976047904191618No Hit
ACGATCTCCTGAGCGTTGGAACTGAACCATGTATCATAGCTGGAGATTTA21.1976047904191618No Hit
TAGACATGTATGACCTCCCCTCGGACGACA21.1976047904191618No Hit
TAGACATGTATGACCTCCCCTCGGACGAC21.1976047904191618No Hit
TTGGCAGTTGGAAATGTAACGTCTGATT21.1976047904191618No Hit
TTGGTACTGAAAGGGTTGGCAGTTGGA21.1976047904191618No Hit
CTCCCCCCGGGCTACAGCACT21.1976047904191618No Hit
AGCGGGCAGAGGGAGAGCTGGAGGAC21.1976047904191618No Hit
TTGGCAGTTGGAAATGTAACGTCTGATTCGGAC21.1976047904191618No Hit
TGCAGAATTGACGGCCCACCAAACATCA21.1976047904191618No Hit
GTGCCAGCTCAAGCAGGAAATAGAGTTGCTGCGTAACGAGCTGCAGCGGGCAGAGGGAGAGCTGGAGGACAGATG21.1976047904191618No Hit
TTCCCCCGGGCT21.1976047904191618No Hit
TGCGTAACGAGCTGCAGCGGGCAGAGGGAGAGCTGGAGGACAGATA21.1976047904191618No Hit
CAGTTGCTGCG21.1976047904191618No Hit
TACAAACGATCTCCTGAGCGCTGGAACTGAA21.1976047904191618No Hit
AGGAAATAGAGTTGCTGCGTAACGAGCTGCAGCGGGC21.1976047904191618No Hit
TTGGCAGTTGGAAATGTAACGTCTGATTTGGA21.1976047904191618No Hit
TCGGAAAAAAACTTGGCGTTTAGGTGTGGTTCACATTCAACATCGGCCCTCACAATTTAGCCACCCTTT10.5988023952095809No Hit
TGCGCCGGACGGACGCGCGCCCACCAAATTAATTT10.5988023952095809No Hit
TGCAGAATTGACGGCCCACCAAAC10.5988023952095809No Hit
GCGCTGGAACTGAACCATGTATCATAGCTGGAGATTTAAATTGCAGAATTGACG10.5988023952095809No Hit
TACAAACGATCTCCTGAGCGCTGGAACTGA10.5988023952095809No Hit
TTGGTACTGAAAGGGATGGCAGTTGGA10.5988023952095809No Hit
ATTTAAATTGCAGAATTGACGGCCCACCAAACATCAAAGAGAATC10.5988023952095809No Hit
TGACGGCCCACCAAACATCAAAGAGAAATTC10.5988023952095809No Hit
TGAAGAAATTACAAACGATCTCCTGAGCGT10.5988023952095809No Hit
GTCTTTGATCTCC10.5988023952095809No Hit
TTTTACTTTACTCCCTTGGTACTGAAAGG10.5988023952095809No Hit
AAGTGCAGAGTGACGGCGCC10.5988023952095809No Hit
GCAGAGTGACGGCGCC10.5988023952095809No Hit
CATTTAAATTGCAGAATTGACGGCCCACCAAACATCAAAGA10.5988023952095809No Hit
ACCCCTCGGACG10.5988023952095809No Hit
AAAAAAAAATCGGAAAAAAACTTGGCGTTTAGGTGTGGTTCACATTCAACATCGGCCCTCACAATTTAGCCACCC10.5988023952095809No Hit
CAACCTGCAGAAGGAGCTGGAACGCGCCCGACAGGAGCAGGAGACGGCGGC10.5988023952095809No Hit
CGGAGCTGGAACGCG10.5988023952095809No Hit
ACGAAGTACACGATATGCATTTTGATTTGAACGCCCATTTTCATAATAAGTCTGGATAACTTTAAAACGTTGT10.5988023952095809No Hit
TTACAAACGATCTCCTGAGCGCTGGAACT10.5988023952095809No Hit
CTGAGAGTTGGAACTGAACCATGTATCATAGCTGGAGATC10.5988023952095809No Hit
CCAAAAGAAAATGATATCACAGACATCGT10.5988023952095809No Hit
CAGGAGCAGGAGACGGCGGCGTCGGAGAAAATCAAACT10.5988023952095809No Hit
TTGACGGCCCACCAAACATCAAAGAGAAATT10.5988023952095809No Hit
CGGCGGCGTCGGAGAAA10.5988023952095809No Hit
CAGACATCGTTGAAGAAATTACAAACGATCTCCTGAGCGTTGGAACTGAACCATGTATCATAGCTGG10.5988023952095809No Hit
TTTATTTTTATT10.5988023952095809No Hit
GAAATCAAACTG10.5988023952095809No Hit
GAGACGGCGGC10.5988023952095809No Hit
TTGGCAGTTGGAAATGTAACGTCTGATTCG10.5988023952095809No Hit
AAGTCCGGCGC10.5988023952095809No Hit
TCTTCTACAGACCCGGTTTGCTCGAATTTT10.5988023952095809No Hit
ATCGTGCACGATATGCATTTTGATTTGAACGCCCATTTTCATAATAAGT10.5988023952095809No Hit
ATAAACAGAATCGAGCAGAATAAACCCAAAAGAAAAGGATATCACAGACA10.5988023952095809No Hit
TTGGTACTGAAAGGGTTGGCAGTTGG10.5988023952095809No Hit
GACGTCTAGCGTCGGTCCTTGTGTACTCGG10.5988023952095809No Hit
AATTACAAACGATCTCCTGAGCGCTGGAACT10.5988023952095809No Hit
AAATTCGGAAAAAAACTTGGCGTTTAGGTGTGGTTCAC10.5988023952095809No Hit
GCCTCCCCTCGGA10.5988023952095809No Hit
TTGGCCGGTAGCGCTGCGCCGGACGGACGCGCGCCCAC10.5988023952095809No Hit
ACTGAACCATGTATCATAGCTGGAGATCATTTAAATTGCAGAATTGACGGCCCACCAAACATCAAAGAGAATCTT10.5988023952095809No Hit
TTGGCAGTTGGAAATGTAACGTCTGATTCGG10.5988023952095809No Hit
AGAATCGAGCAAAATAAACCCAAAAGAAAATGATATCACAGACA10.5988023952095809No Hit
TCGGTCCTTGTGTACTCGGTTTATGAGCAC10.5988023952095809No Hit
TGACGGCCCACCAAACATCAAAGAGAAATT10.5988023952095809No Hit
TTGAACGCCCATTTTCATAATAAGTCTGGATAACTTTAACACGTTGTTGGACTGTGTATCTCTCTATGGTT10.5988023952095809No Hit
TGAAGAAATTACAAACGATCTCCTGAGAGTTGGAACTGAACCATGTATC10.5988023952095809No Hit
TAGGCAGTTGGAAATGTAACGTCTGATTCGGA10.5988023952095809No Hit
CTGGAACTGAACCATGTATCATAGCTGGAAAA10.5988023952095809No Hit
GGTAAATTGCAGAATTGACGGCCCACCAAAC10.5988023952095809No Hit
GGAGACGGCGGCGTCGGAG10.5988023952095809No Hit
TTTGGCCGGTAGCGCTGCGCCGGACGGACGCGCTC10.5988023952095809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)