FastQCFastQC Report
Mon 30 Nov 2020
S34.raw.5721.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.5721.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98
Sequences flagged as poor quality0
Sequence length11-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGAACCTTTACTA1313.26530612244898No Hit
CCTCGAGCTGGC66.122448979591836No Hit
TGAACCTTTACT66.122448979591836No Hit
GTGAACCTTTACT66.122448979591836No Hit
ATGCGGACATTC55.1020408163265305No Hit
TGAACCTTTACTA55.1020408163265305No Hit
CCCGTCCGGCGCA44.081632653061225No Hit
TGTAACTTTTT44.081632653061225No Hit
GAGGGCCGATGTTGAATGTGAACCACACCTAAACGCC33.061224489795918No Hit
ATCCTGTCGGGC33.061224489795918No Hit
GTGAACCTTTAC33.061224489795918No Hit
AGAGCTTCGTGC33.061224489795918No Hit
CGAGCAAACCGGGTCTGTA22.0408163265306123No Hit
TCTCCCTCTGCC22.0408163265306123No Hit
TCGAGCAAACCGGGTCTGTAGAAGATGTG22.0408163265306123No Hit
AGCGCGTCCGTC22.0408163265306123No Hit
CCGTCCGGCGC22.0408163265306123No Hit
TTCGAGCAAACCGGGTCTGTAGAAGATG22.0408163265306123No Hit
TCTGGGCACTG22.0408163265306123No Hit
AATGTGAGCTTC22.0408163265306123No Hit
CCCGTCCGGCGC11.0204081632653061No Hit
TTTATTTTTATT11.0204081632653061No Hit
CCGTCCGGCGCA11.0204081632653061No Hit
ATATTGAAGACAAAGAGAATAAATAAAAATAACTGCATTATAACGTGAAAGTTAAATTAATTTGGTGGGCGCGCG11.0204081632653061No Hit
AATGTTGAATGT11.0204081632653061No Hit
TACATTTCCAACTGCCAACCCTTTCAGT11.0204081632653061No Hit
ACGCCGCCGTCTC11.0204081632653061No Hit
GGATGGTTTGAGAC11.0204081632653061No Hit
AACCTTTACTA11.0204081632653061No Hit
AAAATTCGAGCAAACCGGGTCTGTAGAAGA11.0204081632653061No Hit
CGATGTTGAATGTGAACCACACCTAAACGCCAAGTTTTTTCCCGAATTTTTTTTTGACATTTCTCTTTTTCAGAC11.0204081632653061No Hit
GAGCAAACCGGGTCTGTAGAAGA11.0204081632653061No Hit
GTGAACCTTTACTA11.0204081632653061No Hit
TTGGTGGGCGCGCGTCCGTCCGGCGC11.0204081632653061No Hit
TCTTATTTAAG11.0204081632653061No Hit
ACTTCGTGCACGATATGCATCGTCCAAATGTGCTTACAATCGCAAAAATCGTGGAAAATTCGAGCAAACCGGGTC11.0204081632653061No Hit
CGAGCAAACCGGGTCTGTAGAAGAT11.0204081632653061No Hit
AGCCGGGGGAG11.0204081632653061No Hit
CGAGCAAACCGGGTCTGTAGAAGATG11.0204081632653061No Hit
CAGCCGGGGGAGG11.0204081632653061No Hit
ATGTATCGTCCAAATGTGCGTACAATAGCAAAAATCGTGGAAAAATTCGA11.0204081632653061No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)