FastQCFastQC Report
Mon 16 Nov 2020
S33.raw.12121.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.12121.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234
Sequences flagged as poor quality0
Sequence length11-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATACTAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATGGA5423.076923076923077No Hit
TACTAACACAAGAATAAGAGACAAGCCT3514.957264957264957No Hit
TACTAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATGGA229.401709401709402No Hit
TACTAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATGGATGA93.8461538461538463No Hit
ATGTATACTAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATG83.418803418803419No Hit
TACTAACACAAGAATAAGAGACAAGCCTCCACC52.1367521367521367No Hit
TACTAACACAAGAATAAGAGACAAGCCTCCAC41.7094017094017095No Hit
TAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATGGATGACTCCACCACAAGAAGAAGAGACAAGCCC41.7094017094017095No Hit
TGTATACTAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATGGA41.7094017094017095No Hit
CGGGAGAGGGCC31.282051282051282No Hit
AGATTAGATATTGTACTTTTAGCAGGAAAAGATCAGGGTTGGTCTAGGACAAACGC31.282051282051282No Hit
GAAAGGGACGGGCTATTGTATTCGCTTCC31.282051282051282No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCCCTAACCCACTTAAT31.282051282051282No Hit
CTCCACCACAAGTCAGATGGATGACTCCACCACAAGAAGAAGAGACAAGCCCTAACC20.8547008547008548No Hit
TTTTTAGCCCGTCCCTATTTATAACTTCAAGAAA20.8547008547008548No Hit
AAGGAAAGGGACGGGCTATTGTATTCGCTTCCGTGTCTGTTCGTTCGTCCGTC20.8547008547008548No Hit
AGGAAAGGGACGGGCTA20.8547008547008548No Hit
TGACTCCACCACAAGAAGAAGAGACAAGC20.8547008547008548No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCCCTAACC20.8547008547008548No Hit
TTTTTAGCCCGTCCCTATTTATAACTTCAAGAAAAA20.8547008547008548No Hit
GAAAGGGACGGGCTATTGTATTCGCT20.8547008547008548No Hit
ATGACATGTATACTAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATGGA20.8547008547008548No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCCCTAACCCACTTAATTACTTTCC20.8547008547008548No Hit
TACTAACACAAGAATAAGAGACAAGCC20.8547008547008548No Hit
CTGCCCTTGAATGGTAGAGATTGCCTAAGGCACTAATTTCGCCCTTTGATTGTATAATATTTACTG10.4273504273504274No Hit
TTTTAGCCCGTCCCTATTTATAACTTCAAGAAAAAAA10.4273504273504274No Hit
TTCGCCCTTTGATTGTATAATATTTGCTGT10.4273504273504274No Hit
TTTAGCCCGTCCCTATTTATAACTA10.4273504273504274No Hit
ATGGTAGAGCTTGCCTAAGGCAATAATTTCGCCCT10.4273504273504274No Hit
ATGTATACTAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCA10.4273504273504274No Hit
TATACTAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATGGA10.4273504273504274No Hit
TTAGATCAAAAC10.4273504273504274No Hit
TTATAAAAAGGTAAGGGACGGGCTATTGT10.4273504273504274No Hit
TTTTTAGCCCGTCCCTATTTATAACTTTAAGAAAAAAAAAAAA10.4273504273504274No Hit
TGTTTGTCCTAGTCCACCCCTGATCTTTTCCTGTTAAAGCTACATTCTCTGA10.4273504273504274No Hit
TTTCGCCCTTTGATTGTATAATATTTACTGTC10.4273504273504274No Hit
AGAATTCCAGTTG10.4273504273504274No Hit
AAAAAAAGTTATAAAAAGGAAAGAGACGGGCTATTGT10.4273504273504274No Hit
GTTATAAAAAGGAAAGGGACGGGCTATTGTA10.4273504273504274No Hit
ACGGGCTATTGTATTCGCTTCCGTGTCTGTTCGTTCGT10.4273504273504274No Hit
GTCCCTATTTATAACTTCAAGAAAAAAAAAGTTATAAAAAGGAAAGGGACGGGCTATTGTATTCGCTTCCGTGTC10.4273504273504274No Hit
CACCACAAGAAGAAGAGACAAGCCCTAACCCACTTAATTACTTTCCA10.4273504273504274No Hit
TACTAACACAAGAATAAGAGACAAGCATCCACC10.4273504273504274No Hit
GGGAGAGGGCC10.4273504273504274No Hit
ATGGGAGAGGGCC10.4273504273504274No Hit
TTCGCCCTTTGATTGTATAATATTTACTGT10.4273504273504274No Hit
AAATAATTTCGCCCTTTGACTGTATAATATTTACTGT10.4273504273504274No Hit
TTTTAGCCCGTCCCTATTTATAACTTCAAGAAAAAAAAAAAA10.4273504273504274No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCCCTAACCCACTTAATTACTTTCCAG10.4273504273504274No Hit
AGATTGCCTAAGGCAATAATTTCGCCCTTTGATTGTATAATATTACTG10.4273504273504274No Hit
AAAGAAAGGAATGACAACGCCGGCGCC10.4273504273504274No Hit
ACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATGGATGACTCCACC10.4273504273504274No Hit
AATTTCGCCCTTTGATTGTATACTATTTACT10.4273504273504274No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCCCTAACCCACTTAATTACTTTC10.4273504273504274No Hit
TTTCGCCCTTTGATTGTATAATATTTACTGT10.4273504273504274No Hit
TTAGCCCGTCCCTATTTATAACTTCAAGG10.4273504273504274No Hit
TTTTAGCCCGTCCCTATTTATAACTTCAAGAAAAAAAAAGTTATAAAAAGGAAAGGGACGGGCTATTGTATTCGC10.4273504273504274No Hit
TACTAACACAAGAATAAGAGACAAGCCTC10.4273504273504274No Hit
ATGGTAGAGATTGCCTAAGGCAATAATTTCGCCCTTCGATTGTATAAT10.4273504273504274No Hit
GACTTTTTGCCTGCTTAGGACCAATG10.4273504273504274No Hit
ATAAAAAGGAAAGGGACGGGCTATTGTATTCGCTT10.4273504273504274No Hit
AAAAGGAAAGGGACGGGCTATTGTATTC10.4273504273504274No Hit
TACTAACACAAGAATAAGAGACAATCCT10.4273504273504274No Hit
ACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATG10.4273504273504274No Hit
CTACTTATACAGATGGATGACATGTATACTAACACAAGAATAAGAGACAAGCCT10.4273504273504274No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCCCT10.4273504273504274No Hit
TAAAAAGGAAAGGGACGGGCTATTGTATT10.4273504273504274No Hit
TAATTTCGCCCTTGGATTGTATAATATTTACTGTCAATAAA10.4273504273504274No Hit
GTGTTTTTTAGCCCGTCCCTATTTATAACTTCAAGA10.4273504273504274No Hit
TATACTAACACAAGAATAAGAGACAAGCCTCCACC10.4273504273504274No Hit
GACTAACACAAGAATAAGAGACAAGCCTCCACC10.4273504273504274No Hit
AAACCCTGGGC10.4273504273504274No Hit
TTGTATTCGCTTCCGTGTCTGTTCGTTC10.4273504273504274No Hit
TCCACCACAAGTCAGATGGATGACTCCACCACAAGAAGAAGAGACAAGCCCTAACCCACTT10.4273504273504274No Hit
AAAAGTTATAAAAAGGAAAGGGACGGGCT10.4273504273504274No Hit
TAGAGGGTTGAATGGTAGAGATTACCTAAGGCAATAATTTCGCCCTTTGA10.4273504273504274No Hit
TACTAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCA10.4273504273504274No Hit
AATAACAATCTGACCTAAAGCAAGATTCATTTCTATAGAATTGTATGATGCCCAACCCT10.4273504273504274No Hit
AATTGTATGATGCCCAACCCTAACAGAA10.4273504273504274No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)