FastQCFastQC Report
Mon 16 Nov 2020
S33.raw.12682.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.12682.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267
Sequences flagged as poor quality0
Sequence length11-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGTGGGCCCCGAAAACAGCCAGTCGCAG93.3707865168539324No Hit
ATGCGCATTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGG82.9962546816479403No Hit
TAGAACATTTTACATGCGAAAAATCTTCGCACCCGCCGGGAA72.6217228464419478No Hit
TAGGTGGGCCCCGAAAACAGCCAGTCACAG62.247191011235955No Hit
AGAACATTTTACATGCGAAAAATCTTCGCACCCGCCGGGAA62.247191011235955No Hit
TTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGG41.4981273408239701No Hit
GACTCCCGCGTGCAAGGCGAATGCAGTGATCATCGCGCCATG41.4981273408239701No Hit
TGCAGTGATCATCGCGCCATGGGAGCTG31.1235955056179776No Hit
TCACAGGATAGAACATTTTACATGCGAAAAA31.1235955056179776No Hit
GATAATTAAATTTTACCCACAATGCTGACACCGAGTCGCTATGCGCATTTTATTTCATAGCT31.1235955056179776No Hit
AGATAATTAAATTTTACCCACAATGCTGACACCGATTCGCTATGCGC31.1235955056179776No Hit
TGGGCCCCGAAAACAGCCAGTCACAGGATAG31.1235955056179776No Hit
TTTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGC31.1235955056179776No Hit
AAATCCAAGATAATTAAATTTTACCCACAATGCTGACACCGAGTCGCTATGCGCATTTTATTTCATAG31.1235955056179776No Hit
AGAACATTTTACATGCGAAAAATCTTCGCCCCCGCCGGGAA31.1235955056179776No Hit
TAGAACATTTTACATGCGAAAAATCTTCGCCCCCGCCGGGAATCGAACCCGGGTCTCCCGCGTGCAA31.1235955056179776No Hit
TCGAACCCGGGACTCCCGCGTGCAAGGCGAA31.1235955056179776No Hit
TCATAGCTACCTATTTTCCTCACGAGGCT31.1235955056179776No Hit
ATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGG31.1235955056179776No Hit
TGCGCATTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGA31.1235955056179776No Hit
AACAGCCAGTCACAGGATAGAACATTTTACATGCGAAAAATCTTCGCACCC31.1235955056179776No Hit
CGCATTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGA20.7490636704119851No Hit
AAATTTTACCTACAATGCTGACACCGAGTCGCTATGCGC20.7490636704119851No Hit
GCTGACACCGAGTCGCTATGCGCATTTTATTTCATAGCTACCTATTTTCC20.7490636704119851No Hit
TTAAATTTTACCCACAATGCTGACACCGAGTCGCTA20.7490636704119851No Hit
TAAATTTTACCCACAATGCTGACACCGAGTCGCTATGCGCATTTTTTATTTCATAGCTACCT20.7490636704119851No Hit
CACGGGATAGAACATTTTACATGCGAAAAATC20.7490636704119851No Hit
TACATGCGAAAAATCTTCGACCCCGCCGGGAA20.7490636704119851No Hit
TTTTCCTCACGAGGCTAGGTGGGCCCCGAAA20.7490636704119851No Hit
TTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAACAG20.7490636704119851No Hit
CGAACCCGGGACTCCCGCGTGCAAGGCGAATGCAGTGATCATTGCGCCATGGGAGCTGACTG20.7490636704119851No Hit
CTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAACAT20.7490636704119851No Hit
TGGGCCCCGAAAACAGCCAGTCACAGGATAGA20.7490636704119851No Hit
TTTTACCCACAATGCTGACACCGAGTCGCT20.7490636704119851No Hit
CGCATTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGAAAA20.7490636704119851No Hit
TTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCATAGGA20.7490636704119851No Hit
GTTTTGGAGAC20.7490636704119851No Hit
TTTTACCTACAATGCTGACACCGAGTCGCT20.7490636704119851No Hit
CGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAACATTTTACATGCGAA20.7490636704119851No Hit
TTTTACATGCGAAAAATCTTCGACCCCGCC20.7490636704119851No Hit
TTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAATCACAGG20.7490636704119851No Hit
ATAATTAAATTTTACCCACAATGCTGATACCGAGTCGCT20.7490636704119851No Hit
ATTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAG20.7490636704119851No Hit
TCACAGGATAGAACATTTTACATGCGAAAA20.7490636704119851No Hit
CAATGCTGACACCAAGTCGCTATGCGCATTTTATTTCATAGCTACCTATTTTCCTCACGAGG20.7490636704119851No Hit
AATTAAATTTTACCACAATGCTGACACCGAGTCGCTATGCGC20.7490636704119851No Hit
TATTTTCCTCACGAGGCTAGGTGGGCCCC20.7490636704119851No Hit
ATTACATAGCTACCTATTTCCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACA10.37453183520599254No Hit
TTTACCCACAAAGCTGACACCGAGTCGCTATGC10.37453183520599254No Hit
ATGCTGACACCGAGTCGCTATGCGCATT10.37453183520599254No Hit
TGCGCATTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGC10.37453183520599254No Hit
ACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAACA10.37453183520599254No Hit
ATGTTTTGGAGACGTTGCACTTGGCTT10.37453183520599254No Hit
CATTATATTTTCCTCACGAGGCTAGGTGGGCCCCGAA10.37453183520599254No Hit
TACATGCGAAAAATCTTCGACCCCGCC10.37453183520599254No Hit
AACATTTTACATGCGAAAAATCTTCGCCCCCGCCG10.37453183520599254No Hit
TGCAGTGATCATCGCGCCATGGGAGCT10.37453183520599254No Hit
CAAGATAATTAAATTTTACACACAATGCTGACACCGAGTCGCTATGCGC10.37453183520599254No Hit
TATTTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGA10.37453183520599254No Hit
TTTACCCACAATGCTGACACCGAGTCGCT10.37453183520599254No Hit
ACTCCCGCGTGCAAGGCGAATGCAGTGATCATCGCGCCA10.37453183520599254No Hit
CTGACACCAAGTCGCTATGCGCATTTTATTTCATAGCTACCTATTTTCCT10.37453183520599254No Hit
AACATTTTACATGCGAAAAATCTTCGCACCCGCCGGGAA10.37453183520599254No Hit
TTTTACCCACAATGCTGATACCGAGT10.37453183520599254No Hit
AAAAAAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGG10.37453183520599254No Hit
TTAAACATTTTACATGCGAAAAATCTTCGCCCCCGCCGGGAATCGAA10.37453183520599254No Hit
TGGGCCCCGAAAACAGCCAGTCACAGGATAGAACAT10.37453183520599254No Hit
ATGCTGACACCAAGTCGCTATGCGCATTTTATTTCATAGCTACCTATTTTCCT10.37453183520599254No Hit
TGGGCCCCGAAAACAGCCAGTCACAGGATAGAA10.37453183520599254No Hit
ATTTTACCCACAATGCTGACACCAAGTCGCTATGCGCAT10.37453183520599254No Hit
AGAACATTTTACATGCGAAAAATCTTCGACCCCGCCGGGAATCGAACCCGA10.37453183520599254No Hit
CTCCCGCGTGCAAGG10.37453183520599254No Hit
GCGCATATTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCC10.37453183520599254No Hit
AAATCCAAGATAATTAAATTTTACCACAATGCTGACACCGAGTCGCTATGCGCATTTTATTTCATAGCTACCTAT10.37453183520599254No Hit
TTTTCCTCACGAGGCTAGGTGGGCC10.37453183520599254No Hit
ATCCAAGATAATTCAATTTTACCCACAATGCTGACACCAAGTCGCTATGCGCATTTTATTT10.37453183520599254No Hit
AGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAACATT10.37453183520599254No Hit
CTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGGTAGAACAT10.37453183520599254No Hit
TTTTACCCACAATGCTGACACCGAGTCGCTATGCGCATTTTATTTCATAGC10.37453183520599254No Hit
AAATTTTACCCACAATGCTCACACCGAGTCGCT10.37453183520599254No Hit
TTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAACATTTTAC10.37453183520599254No Hit
ATTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGA10.37453183520599254No Hit
CATTTTACATGCGAAAAATCTTCGACCCCGCCGGGAATCGAACCCGGGTC10.37453183520599254No Hit
ATTTTACATGCGAAAAATCTTCGCCCTCGCCGGGAATCGAACTC10.37453183520599254No Hit
TATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGAAAAC10.37453183520599254No Hit
CCTATTTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGGTAGAACATTTA10.37453183520599254No Hit
TTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCATAGGATAGAAC10.37453183520599254No Hit
CGCACCCGTCGGGAATCGAACTCGGGACTCCCGCGTGCAAGGCGAATGCA10.37453183520599254No Hit
TTTTACCTACAATGCTGACACCGAGTCGCTATGCGCATTTTATTTCATAGCTACCT10.37453183520599254No Hit
TAGCTACCTATTTTCCTCACGAGG10.37453183520599254No Hit
CCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCATAGGATAGAACATTTTACATG10.37453183520599254No Hit
CCAGTCACAGGATAGAACATTTTACATGCGAAAAATCTTCGCCCCCGCCGGGAATCGAACCCGGGTCTCCCGCG10.37453183520599254No Hit
TTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGG10.37453183520599254No Hit
AGACTCCCGCGTGCAAGGCGAATGCAGTGATCATCGCGCCATG10.37453183520599254No Hit
TAAATTTTACCCACAATGCTGACACCGAGTCGC10.37453183520599254No Hit
TTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAACATTTTACATGCGAA10.37453183520599254No Hit
ACAAAGCTGACACCGAGTCGCTATGCGCATTTTATTTCATAGCTACCTAT10.37453183520599254No Hit
ACATTTTACATGCGAAAAATCTTCGCACCCGCCGGGAA10.37453183520599254No Hit
AGGATTTGCCAAGCTCATAATTCCAAGATAATTAAATTTTACCCACAATGCTGACACCGAGTCGCTATG10.37453183520599254No Hit
AACATTTTACATGCGAAAAATCTTTGCCCCCGCCGGGAATCGAACCCGGGACTCCCGCGTGCAAGGCAAATGCAG10.37453183520599254No Hit
CAGTCACAGGATAGAACATTTTACATGCGAAAAATCTTCGCACCCGCCGGGA10.37453183520599254No Hit
CCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAAC10.37453183520599254No Hit
TCATAGCTACCTATTTTCCTCACGAGGATA10.37453183520599254No Hit
TTATTTCATAGCTACCTATTTTCCTCACGA10.37453183520599254No Hit
AGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAACATTTTACATGCGAAAAATCTTCGCACCCGCCGG10.37453183520599254No Hit
TTTTACCCACAAAGCTGACACCGAGTC10.37453183520599254No Hit
TTAAATTTTACCCACAATGCTGACACCGAGTCGCTATGCGC10.37453183520599254No Hit
CCTATTTTCCTCACGAGGCTAGGTGGGCC10.37453183520599254No Hit
GAATCGAACCCGGGACTCCCGCGTGCAAGGCGAA10.37453183520599254No Hit
AGTCACAGGATAGAACATTTTACATGCGAAAAATCTTCGCCCCCGCCGGGAATCGAACCCGGGACTCCCGCG10.37453183520599254No Hit
ACATTTTACATGCGAAAAATCTTCGACCCC10.37453183520599254No Hit
TTTTACATGCAAAAAATCTTCGCCCCCGCC10.37453183520599254No Hit
AAAATCTTCGCCCCCGCCGGAAATCGAAC10.37453183520599254No Hit
TTCCTCACGAGGATAGGTGGGCCCCGAAA10.37453183520599254No Hit
TTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAACA10.37453183520599254No Hit
ATTTTACCCACAAAGCTGACACCGAGTCGCT10.37453183520599254No Hit
TTTACCCACAATGCTGACACCGAGTTGCTATGCGCATTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGG10.37453183520599254No Hit
ATTTTCCTCACGAGGCTAGGTGGG10.37453183520599254No Hit
AATTAAATTTTACCCACAATGCTGACACCGAGTCGCTATGCGCATTTTTT10.37453183520599254No Hit
AACAGCCAGTCACAGGATAGAACATTTTACATGCGAAAAATCTTCGCACCCGCCGGGAAT10.37453183520599254No Hit
CGTGGAGGACTTCCA10.37453183520599254No Hit
ATCCAAGATAATTAAATTTTACACACAATGCTGACACCGAGTCGCTATGCGC10.37453183520599254No Hit
TTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGTCAGTCACAG10.37453183520599254No Hit
CTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAAGA10.37453183520599254No Hit
TCTTCGCCCCCGCCGGGAATCGAACCCGA10.37453183520599254No Hit
ATTTTACCCACAATGCTGACACCGAGTTGCTATGCGCATTTTATTTCATA10.37453183520599254No Hit
AGCCAGTCACAGGATAGAACATTTTACATGCGAAAAATCTTCGCACCCGCCGGGAATC10.37453183520599254No Hit
TTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGT10.37453183520599254No Hit
TAGAACATTTTACATGCGAAAAATCTTCGACCCCGCCGGGAATCGAACCC10.37453183520599254No Hit
GGCCCCGAAAACAGCCAGTCACAGGATAGAACATTTTACATGCGAAAAAT10.37453183520599254No Hit
ATTTTACCTACAATGCTGACACCGAGTCGCTATGCGCATTTTATTTCATAGCTACCTATTTTCCTCGTGA10.37453183520599254No Hit
GCGCATTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAG10.37453183520599254No Hit
TTACCCACAATGCTGACACCGAGTCGCTATGCGC10.37453183520599254No Hit
CGCATTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCC10.37453183520599254No Hit
TAATTCAATTTTACCCACAATGCTGACACCAAGTCGCTATGCGCATTTTA10.37453183520599254No Hit
CAGTCACAGGATAGAACATTTTACATGCGAAAAATCTTCGCCCCCGCCGGGAATCGA10.37453183520599254No Hit
TTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAACAT10.37453183520599254No Hit
TTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAACATT10.37453183520599254No Hit
TTCGCCCCCGCC10.37453183520599254No Hit
ATTAAATTTTACCCACAATGCTGACACCGAGTCGCTATG10.37453183520599254No Hit
GCGCATTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGAA10.37453183520599254No Hit
ATGCAGTGATCATCGCGCCATGGGAGCTG10.37453183520599254No Hit
AATCGAACTCGAGACTCCCGCGTGCAAGGC10.37453183520599254No Hit
TTAAATTTTACCCACAATGCTGACACCGAGTCGCT10.37453183520599254No Hit
ATAAACCCAAGATAATTAAATTTTACCCACAATGCTGACACCGAGTCGCTATGCGCATTTTATTTCATAGCTACC10.37453183520599254No Hit
CAAGATAATTAAATTTTAGCAACAATGCTGACACCTAGTCGCTATGCGCA10.37453183520599254No Hit
AGAACATTTTACATGCGAAAAATCTTCGCACCCGCCGGGAAA10.37453183520599254No Hit
TCACAGGATAGAACATTTTACATGCGAAAAAT10.37453183520599254No Hit
CGCCCCCGCCGGG10.37453183520599254No Hit
TGGGCCCCGAAAACAGCCAGTCACAGGATAGAACATTTTACATGCGAAAAATCTTCGCACCCGCAA10.37453183520599254No Hit
GGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAACAGTTTACAT10.37453183520599254No Hit
TCGAACTCGGGACTCCCGCGTGCAAGGCGA10.37453183520599254No Hit
GTTTACATGCGA10.37453183520599254No Hit
TTTTACCCACAATGCTGACACCGAGTCGCGATGCGCAA10.37453183520599254No Hit
TCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAAAACATTTTAC10.37453183520599254No Hit
AATGCGCATTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCATAGGA10.37453183520599254No Hit
TTTATTTCATAGCTACCTATTTTCCTCACGATGCTAGG10.37453183520599254No Hit
ATTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAACA10.37453183520599254No Hit
GAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAGAACATTTTAC10.37453183520599254No Hit
TCCCGCGTGCAAGGCGAATGCAGTGATC10.37453183520599254No Hit
TTTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCACAGGATAG10.37453183520599254No Hit
TTCCTCACGAGGCTAGGTGGGCCGCGAAAACAGCCAGTCACAGG10.37453183520599254No Hit
TTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGAAA10.37453183520599254No Hit
TGCTGATACCGAGTCGCTAATGCGCATTTTATTTCATAGCTACCTATTTT10.37453183520599254No Hit
TACCCACAATGCTGACACCAAGTCGCTATGCGCA10.37453183520599254No Hit
CCCGCCGGGAATCGAACCCGGGTCTCCCGCGTGCAAGGCGAATGCAGTGA10.37453183520599254No Hit
ACTCCCGCGTGCAAGGCGAATGCAGTGATCATCGCGCCATG10.37453183520599254No Hit
TTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCATAGGAAAGAACATT10.37453183520599254No Hit
AATTAAATTTTACCTACAATGCGGACACCGAGTCGCTATGCGCATTTTATTTCATAGCTACC10.37453183520599254No Hit
TTAAATTTTACCCACAATGCTGACACCGAG10.37453183520599254No Hit
TCGAACCCGGGACTCCCGCGTGCAAGGCGA10.37453183520599254No Hit
ATTTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCATAGAAAAAAA10.37453183520599254No Hit
TTTATTTCATAGCTACCTATTTTCCTCACGAGGCTAGGTGGGCCCCGAAAACAGCCAGTCATAGGATAGAAC10.37453183520599254No Hit
ATTCAATTTTACCCACAATGCTGACACCAAGTCGCTATGCGCATTTTATTTCATAG10.37453183520599254No Hit
TTTACCCACAAAGCTGACACCGAGTCGCTAT10.37453183520599254No Hit
TCATAGCTACCTATTTTCCTCACGAGGAT10.37453183520599254No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)