FastQCFastQC Report
Mon 16 Nov 2020
S33.raw.22483.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.22483.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109
Sequences flagged as poor quality0
Sequence length11-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGAAAAGCTTACTCGGCCTGTGCATC3330.275229357798167No Hit
TCGGCAACTTTTTCAGAGCGCGTGCGAAA76.422018348623854No Hit
TCAGGGGTCGGCAACTTTTTCAGAGCGC54.587155963302752No Hit
AACGTGTACACCTCGTTCTAATTCTATTTACGCCGTACC43.669724770642202No Hit
TACATCAGGGGTCGGCAACTTTTTCAGAGC43.669724770642202No Hit
TCGGCAACTTTTTCAGAGCGCGTGCGAAAA32.7522935779816518No Hit
TTCTGCTGCTAAGTCTGCTCGTACTGAAGG32.7522935779816518No Hit
TTCTACAAATCCTACGGAATCAAGCTCAAC21.834862385321101No Hit
TCGTACTGAAGGAACTGGGGAAACTGTG21.834862385321101No Hit
TCAAAGAAAAGCTTACTCGGCCTGTGCATCA21.834862385321101No Hit
TCGGCTAAGTCTACACCACCATATTTGTA21.834862385321101No Hit
CAGGCATACATAAGAAGCGCAATGGTTCCACC21.834862385321101No Hit
TACATAAGAAGCGCAATGGTTCCACCTAGG21.834862385321101No Hit
GCAAACAAGAAAAAGATCGGCCATGGCAG10.9174311926605505No Hit
TCCCTAGGAAATTCGTCGGCCTCACAAGT10.9174311926605505No Hit
ATTATATCCTTCACTCAGTGGTTTAGAAGAGCAAACAAGAAAAAGATCGG10.9174311926605505No Hit
TGGTCCCAACTGGTTTGTCTGATATGAGT10.9174311926605505No Hit
TCTATCGGCTAAGTCTACACCACCATATT10.9174311926605505No Hit
TACAACTGTGGAACGGAGCGATTGGGACGC10.9174311926605505No Hit
TGAAGGAACTGGGGAAACTGTGTCTGCT10.9174311926605505No Hit
ATGGTCCCAACTGGTTTGTCTGATATGAGT10.9174311926605505No Hit
TAGGAAATTCGTCGGCCACACAAGTGTG10.9174311926605505No Hit
ATCCCTAGGAAATTCGTCGGCCTCACAAGT10.9174311926605505No Hit
TCCACCCAAGATCAAAATTTTGTAAACTGTT10.9174311926605505No Hit
TACTGAAGGAACTGGGGAAACTGTGTCT10.9174311926605505No Hit
TAGGAAATTCGTCGGCCACACAAGTGT10.9174311926605505No Hit
AATACAACTGTGGAACGGGGCGATTGGGA10.9174311926605505No Hit
TGCGGTACAGGGCAATCATTCTATCGGCC10.9174311926605505No Hit
TCTACGTAGCGGAGGAGGTGCGAGTGCT10.9174311926605505No Hit
AAGCCGCAGCC10.9174311926605505No Hit
TTACTCCTTTGTCGTATATACCTGTAGACT10.9174311926605505No Hit
TACATAAGAAGTGCAATGGTTCCACCTAGG10.9174311926605505No Hit
TCAAACTCCACCTTACCTTTTCGTCAATGG10.9174311926605505No Hit
TACAAATCCTACGGAATCAAGCTCAACAGA10.9174311926605505No Hit
TCGGCAACTTTTTCAGAGCGCGTGCAAAAACAACTTT10.9174311926605505No Hit
TAAATTTTGCGAGGTAGACGTAACGTCGGC10.9174311926605505No Hit
TACCTCAATTAAATCCTTCCACCCAAGATC10.9174311926605505No Hit
TATAAAAAATCAAACTCCACCTTACCTTT10.9174311926605505No Hit
TCTATTGCAACGACTGCACGCAAACGAGGC10.9174311926605505No Hit
AGGAAGCGTAAACCCAGAGAACCGTTGGAGATCAAGCGACCTTCCAGTTA10.9174311926605505No Hit
TACGAACCTCACTTCGGAATTGTCAAAGCACCGGAT10.9174311926605505No Hit
AGGAAGCGTAAACCCAGAGAACCGTTGGC10.9174311926605505No Hit
TACAACTGTGGAACGGGGCGATTGGGAC10.9174311926605505No Hit
TTCCACCGAAGATCAAAATTTTGTAAACTGT10.9174311926605505No Hit
TCAGGGGTCGGCAACTTTTTCAGAGC10.9174311926605505No Hit
TAGGAAGCGTAAACCCAGAGAACCGTTGGC10.9174311926605505No Hit
CACAATGTCGGCGACACAGGTGGCGAGTCTT10.9174311926605505No Hit
TCCTACGGAATCAAGCTCAACAGACCCTTC10.9174311926605505No Hit
GCGAGTGCTGTATCCCTAGGAAATTCGT10.9174311926605505No Hit
TAGGAAATTCGTCGGCCTCACAAGTGTGGATA10.9174311926605505No Hit
CCACAAGTTCATGTTGGTATCAGCGCTAT10.9174311926605505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)