FastQCFastQC Report
Mon 16 Nov 2020
S33.raw.2854.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.2854.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277
Sequences flagged as poor quality0
Sequence length11-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACGGCGCTGTTGGGTGGTCCCGAAGCC1148.927173061863742No Hit
TGAACCGGAACCACAGAACTCAGAGTCACT584.541895066562255No Hit
TATACGTCGTGTATGATGCGAGGCCTTGG372.8974158183241974No Hit
TCACCGTCGTTC362.8191072826938135No Hit
TAACGGCGCTGTTGGGTGGTCCCGAAGCC322.505873140172279No Hit
TTCTAGATCTGCCGAGAACTTTCCCTTACC231.8010963194988252No Hit
TCTGAACCGGAACCACAGAACTCAGAGTC191.4878621769772904No Hit
TTAACGGCGCTGTTGGGTGGTCCCGAAG191.4878621769772904No Hit
TACCCAATCGCCATGTACGCCCTCCGACT181.4095536413469067No Hit
TGAGTGTCTTTTCTGAGTGGTCCCACCTCCCCCGCCTGAAGGTGGTCCATCAGGGC171.331245105716523No Hit
TACGGCGGTAACAATGTCCCAGAGGCTGC151.1746280344557558No Hit
TGAACCGGAACCACAGAACTCAGAGTCACTG131.0180109631949883No Hit
TGTTCCAGGCTCCCCACTCGTCTTCTGTG120.9397024275646046No Hit
TAGGCCAGGAAATTGTTCATCGTCTGAACCGG120.9397024275646046No Hit
TCGTCTGAACCGGAACCACAGAACTCAG110.8613938919342208No Hit
TCTAGATCTGCCGAGAACTTTCCCTTACC110.8613938919342208No Hit
TACCCTATTATCTCGTCTGTCCCCAAAGT90.7047768206734534No Hit
TTAACGGCGCTGTTGGGTGGTCCCGAAGC90.7047768206734534No Hit
ACCGGAACCACAGAACTCAGAGTCACTGTT90.7047768206734534No Hit
TTTGTAGAATGGAAGTTAGCGGCGCTGTT90.7047768206734534No Hit
TTTTCAACAAGGTTCTTGGTTGGTTGGG80.6264682850430697No Hit
TGTTTGAGGACTCCACGCTGAAATGCCCT80.6264682850430697No Hit
TCTTATAATACGCCAGCGAAGGATCGTTCC80.6264682850430697No Hit
TCAAAAAAGAAGTTCTTTCCCGCGTTGGA70.548159749412686No Hit
AGTCGTCGTCGTG70.548159749412686No Hit
TACCTGTCCACTGCTGTACGAAGGCCTCT70.548159749412686No Hit
TTTGACTACCCAATCGCCATGTACGCCCTC70.548159749412686No Hit
TCAGCCGTTACCTGTCCACTGCTGGACGAA70.548159749412686No Hit
TTCACCGTCGTTC60.4698512137823023No Hit
TTGTAGAATGGAAGTTAGCGGCGCTGTT60.4698512137823023No Hit
TAGGCCAGGAAATTGTTCATCGTCTGAACC60.4698512137823023No Hit
TACGCCCTCCGACTTGAAGGTACACAGCCT60.4698512137823023No Hit
TTGTCGCATTAAGAAAAGATTCCCTACCC60.4698512137823023No Hit
TCCCTTACCATACTTCCACGACAAACAGCT60.4698512137823023No Hit
TATACGTCGTGTATGATGCGAGGCCTTGGA60.4698512137823023No Hit
TCCACGCTGAAATGCCCTGACCCGCCTTATC60.4698512137823023No Hit
TGTTCCAGGCTCCCCACTCGTCTTCTGTGT60.4698512137823023No Hit
TTCTAGATCTGCCGAGAACTTTCCCTTAC60.4698512137823023No Hit
TCGTCTGAACCGGAACCACAGAACTCAGA60.4698512137823023No Hit
GAGGACTCCACGCTGAAATGCCCTGACCCG50.39154267815191857No Hit
TTATACAACCTCCCCACCAATAGCCAACAT50.39154267815191857No Hit
TAGGCACAATCGTGGACGACGTGGAGGT50.39154267815191857No Hit
TTTTTCAACAAGGTTCTTGGTTGGTTGGG50.39154267815191857No Hit
TTCGCCGCAAAAGTCACCGTCGTTCTCATAGT50.39154267815191857No Hit
TCCCAGAGGCTGCTCCGACAAACATTACAT50.39154267815191857No Hit
TCAATTCCAAAGCAAAATTGTTGAACAAGTTGGTTAGCATGCTGGACGTTCGCAAAACT50.39154267815191857No Hit
TGAGGACTCCACGCTGAAATGCCCTGACCC50.39154267815191857No Hit
TCAGAGTCACTGTTTGCTGAGGCAATCTT50.39154267815191857No Hit
TGAGGACTCCACGCTGAAATGCCCTGACC50.39154267815191857No Hit
TGCAGAAATACCGCTGGCAGAAGCTCCACT50.39154267815191857No Hit
AACCACAGAACTCAGAGTCACTGTTTGCTG40.31323414252153486No Hit
TGTTAACAAGTGTTCCAGGCTCCCCACTC40.31323414252153486No Hit
TAAAAGGATCTGTTCGGGTAATGAGTGTC40.31323414252153486No Hit
TTACCTAAATTTCCTCTACCATTCCTCGG40.31323414252153486No Hit
TACGCCAGCGAAGGATCGTTCCCTAAACTC40.31323414252153486No Hit
TTTTCAACAAGGTTCTTGGTTGGTTGGGA40.31323414252153486No Hit
TGATTGTCGTCGTCGTGGTCAGCCGTTA40.31323414252153486No Hit
TGTTGTATCGACACCCGGCTGGGCCTCTC40.31323414252153486No Hit
TTTGCGCCGAACCCGTTACATAGTTAGATCTC40.31323414252153486No Hit
TCCACGCTGAAATGCCCTGACCCGCCT40.31323414252153486No Hit
TTCCCTACCCGGATGTATCTGTTTATCGACT40.31323414252153486No Hit
TCGGTTATTACTCTAAATGCCCCGCAAAAC40.31323414252153486No Hit
TGAACCGGAACCACAGAACTCAGAGTCACTGT40.31323414252153486No Hit
TGCGCCGAACCCGTTACATAGTTAGATCTC40.31323414252153486No Hit
TAGGCCAGGAAATTGTTCATCGTCTGAAC40.31323414252153486No Hit
TCCGAATCGATATTGTCGAATAACCCACCT40.31323414252153486No Hit
TAGAATACAGCACCAGCACCAGCAGATGA30.23492560689115116No Hit
TACGTCGTGTATGATGCGAGGCCTTGG30.23492560689115116No Hit
TACGTCGTGTATGATGCGAGGCCTTGGA30.23492560689115116No Hit
TATGCCACCAACTAATGCTTGACTAAGTGTACCTGTTTCCTGAGA30.23492560689115116No Hit
TACCCAATCGCCATGTACGCCCTCCGACTT30.23492560689115116No Hit
TCAACTTCTCCGAATACCCGTTGGCGTCTAAAGCTACAAACATCCCAGTAGCGGGAAACTGTGTCTCAATA30.23492560689115116No Hit
TTCCCTACCCGGATGTATCTGTTTATCGAC30.23492560689115116No Hit
TACCTACCAAGCCGAAGTTACAATAAAAAT30.23492560689115116No Hit
TACATATACCCTATTATCTCGTCTGTCCCC30.23492560689115116No Hit
TTCCACGAGTGCCACATAATCCCTAAGGG30.23492560689115116No Hit
TGAACGGAGCCAGCTGCATCTCACCCTCTT30.23492560689115116No Hit
TTATAATACGCCAGCGAAGGATCGTTCCC30.23492560689115116No Hit
TTATCTCGTCTGTCCCCAAAGTACATAATT30.23492560689115116No Hit
TAAGTTCGGTACGACTTTCGGAAGAGGT30.23492560689115116No Hit
TACACGTGCCTCGCGATAACAGTTGGCG30.23492560689115116No Hit
CTGAACCGGAACCACAGAACTCAGAGTCACT30.23492560689115116No Hit
TATAGAATACAGCACCAGCAGCAGCAGAT30.23492560689115116No Hit
TCTGAACCGGAACCACAGAACTCAGAGTCACT30.23492560689115116No Hit
TTAGAGAGAGGACCCAACAACATTGTCGC30.23492560689115116No Hit
TAACGGCGCTGTTGGGTGGTCCCGAAG30.23492560689115116No Hit
TAATGAGTGTCTTTTCTGAGTGGTCCCACC30.23492560689115116No Hit
TACGTCGTGTATGATGCGAGGCCTTGGAG30.23492560689115116No Hit
TCATCTTCTCCACCTAGTCCCTCAAATCT30.23492560689115116No Hit
TTCCCTTACCATACTTCCACGACAAACAGC30.23492560689115116No Hit
GAACCGGAACCACAGAACTCAGAGTCACT30.23492560689115116No Hit
CAGGCTCCCCACTCGTCTTCTGTGT30.23492560689115116No Hit
TATGGACACCCGGCTGGGCCTCTCTCA30.23492560689115116No Hit
ACCGGAACCACAGAACTCAGAGTCACTGT30.23492560689115116No Hit
TCCTTCTTGTTCTTCGTGGCGGCAGCCGA30.23492560689115116No Hit
TTGAGGACTCCACGCTGAAATGCCCTGACC30.23492560689115116No Hit
TCAATATCGACAACGTGCACCGGTGCAAGGGA30.23492560689115116No Hit
TATACCCTATTATCTCGTCTGTCCCCAAAG30.23492560689115116No Hit
ATATCGTCGTCGTGGTCAGCCATTACCTGTCCA30.23492560689115116No Hit
TATAGAATACAGCACCAGCAGCAGCAGATG20.15661707126076743No Hit
AACGATCCGACCTGTTGTATCGACACCCGGC20.15661707126076743No Hit
TCTGAACCGGAACCACAGAACTCAGAGTCACTGTTTGCTGAGGCAA20.15661707126076743No Hit
TCCCTAATGAACGGAGCCAGCTGCATCTC20.15661707126076743No Hit
TTCCCTTACCATACTTCCACGACAAACAG20.15661707126076743No Hit
TGAACCGGAACCACAGAACTCAGAGTCACTGTT20.15661707126076743No Hit
TCTGCCGAGAACTTTCCCTTACCATACTTCC20.15661707126076743No Hit
TCCCACCTCCCCCGCCTGAAAGTGGTCCATC20.15661707126076743No Hit
TAGAATGGAAGTTAGCGGCGCTGTTGGG20.15661707126076743No Hit
TGAAGACCATACCCTGTACCAAAAGAATAGTATTCTTCGTGGACTT20.15661707126076743No Hit
TTGATTGTCGTCGTCGTGGTCAGCCGTT20.15661707126076743No Hit
TGAAATATACGTCGTGTATGATGCGAGGCCTTGGAGGCACTCCTTGAAGGAGTG20.15661707126076743No Hit
CTTGAGATCTCAATAAGTTCAAAGACGTCACGTCCTCGTCTTTAGAGAGA20.15661707126076743No Hit
TACCCGGATGTATCTGTTTATCGACTAAGT20.15661707126076743No Hit
TACTTCCACGACAAACAGCTGACCGCTCTT20.15661707126076743No Hit
TATAGAATACAGCACCAGCACCAGCAGAT20.15661707126076743No Hit
CACTGTTTGCTGAGGCAATCTTGGAAGTTTACAAAAATAACACGGGCAAT20.15661707126076743No Hit
TATCGACACCCGGCTGGGCCTCTCTCAGT20.15661707126076743No Hit
TGACCGCTCTTATCAACTTCTCCGAATACC20.15661707126076743No Hit
TAAAAATGTATAATACAATCAAAAAAGAAGTTCTTTCCCGCGTTGGAAG20.15661707126076743No Hit
TCTTGCCACCAGTCCTTCTTGTTCTTCGT20.15661707126076743No Hit
TGAACCGGAATCACAGAACTCAGAGTCACT20.15661707126076743No Hit
TACCTGTTGAAATTACCTACCAAGCCGAAGT20.15661707126076743No Hit
AGAGTAATAGTTGCTCCAGAATTGATAGACTGTCCTCAATTGAAAAATAT20.15661707126076743No Hit
TACTAATGGCCTCATTTCTTTCGGCGAC20.15661707126076743No Hit
TTTGACTACCCAATCGCCATGTACGCCC20.15661707126076743No Hit
TCTTCGTCGATACCCAACCTTCTAAGGATCCATTTCTCTATGGCTTGTGT20.15661707126076743No Hit
TTCAACGTTTCTAAGGCCGTCGGTTCAGCT20.15661707126076743No Hit
TCTTCATTTCCTCTCCATTGAGTTCTATCC20.15661707126076743No Hit
TCTGAACCGGAACCACAGAACTCAGAGTCAC20.15661707126076743No Hit
TGTTTGAGGACTCCACGCTGAAATGCCC20.15661707126076743No Hit
TTTTCCGATCCTCTTTTTATACAACCTCCC20.15661707126076743No Hit
ACCGGAACCACAGAACTCAGAGTCACT20.15661707126076743No Hit
TACGCCCTCCGACTTGAAGGTATACAGCCT20.15661707126076743No Hit
GAAACAGATAGGCCAGGAAATTGTTCATCGTCTGAACCGGAAC20.15661707126076743No Hit
TCATAACTCTTTCTAGCCATGTCCGCATA20.15661707126076743No Hit
TAACCCACAAAACTCTGCGTCATCTGTGATAGCAACTCTAATTTTCAAAGGACACTCCACGTAGA20.15661707126076743No Hit
TGTTTGATCCCCACCAAGTAACGTTTTCA20.15661707126076743No Hit
ATGGTCTCTATTCTCAGCTGCAAGGGTT20.15661707126076743No Hit
TTACCTAAATTTCCTCTACCATTCCTGGG20.15661707126076743No Hit
TTCCTTCTGGTGACCTGACGTTTCAACTCCACT20.15661707126076743No Hit
TGTTTGAGGACTCCACGCTGAAATGCCCTGACCCGCCT20.15661707126076743No Hit
GTGGCTCCCCACTCGTC20.15661707126076743No Hit
TCCCGTTGTTAACAAGTGTTCCAGGCTCCC20.15661707126076743No Hit
TGAGTGTCTTTTCTGAGTGGTCCCACCTC20.15661707126076743No Hit
TCCTTAATGTGAAGTGAAGACCATACCCTG20.15661707126076743No Hit
CTACCCTGGTTAGCAATTTTGACTCTTTCAAAAGCACC20.15661707126076743No Hit
TGTTGTATCGACACCCGGCTGGGCCTCTCC20.15661707126076743No Hit
TTGAAAAATATGTTTCCCGGATATTGCCC20.15661707126076743No Hit
TGTGTAGGCGCAATCGTGGACGACGTGG20.15661707126076743No Hit
TGGGTCGAGTACGGAGGGCGTCTTGTATGC20.15661707126076743No Hit
TAAGAAAAGATTCCCTACCCGGATGTATC20.15661707126076743No Hit
TCCCTACCCGGATGTATCTGTTTATCGACT20.15661707126076743No Hit
TTAGCAATTTTGACTCTTTCAAAAGCACCTACTATGCCACCAACTAATGCT20.15661707126076743No Hit
TTTACCTAAATTTCCTCTACCATTCCTCGG20.15661707126076743No Hit
TGAAGACCATACCCTGTACCAAAAGAATA20.15661707126076743No Hit
AACATGGTCTCTATTCTCAGCTGCAAGGG20.15661707126076743No Hit
TAGGCCAGGAAATTGTTCATCGTCTGAACCGGAACCACAGAAC20.15661707126076743No Hit
TCAAACACTACTCCGTTGAACCATCCGACC20.15661707126076743No Hit
TCCATTACCCTATCCGGATGCTTGCAGCCT20.15661707126076743No Hit
TCTTTTCTGAGTGGTCCCACCTCCCCCGCC20.15661707126076743No Hit
TATCCGGATGCTTGCAGCCTCTTTTAACA20.15661707126076743No Hit
TCTTACTAATGGCCTCATTTCTTTCGGCGAC20.15661707126076743No Hit
TAGAATGGGCGGATTCTGTCATTGACA20.15661707126076743No Hit
TTCGATTCGGACCAATGACAAGTTTGTAGAA20.15661707126076743No Hit
TGTACCGGTGCAAGGGCCCTAATCTTACT20.15661707126076743No Hit
TTCCCTAAACTCGCGTTCATTTCTGCCCT20.15661707126076743No Hit
TGTTGTACACTTCGGTTGCACGACCCAATGCCGAATTCGCCGCAAAAGTCACCG20.15661707126076743No Hit
TATAGAATACAGCACCAGCACCAGCAGATGATAA20.15661707126076743No Hit
TAATGAGTGTCTTTTCTGAGTGGTCCCA20.15661707126076743No Hit
AACGATCCGACCTGTTGTATCGACACCCGG20.15661707126076743No Hit
TATGTTCTTCAGCGTAAATCTAACTGTACGTCCTTCGACGTCATCTTCTCCACCTAGTCCCTCAAATCTACGA20.15661707126076743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)