FastQCFastQC Report
Mon 16 Nov 2020
S33.raw.3449.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.3449.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312
Sequences flagged as poor quality0
Sequence length11-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGACGTCCCCCCTTAACATTACTGCCCT8226.282051282051285No Hit
TGTGACGTCTCCCCTTAACATTACTGCCCT185.769230769230769No Hit
GGTGCTTAGGTCCTCCTGTTCTAACTAACT175.448717948717949No Hit
TCCCAGAGGTGACTTGCCTGAACATGTT82.564102564102564No Hit
TAACATTACTGCCCTTAAGAGTTCTTCCGG72.2435897435897436No Hit
TTAGAATCGTAGTCTGGGGGGAACAGCCT72.2435897435897436No Hit
TTTGAATCCACACCTATCATAACCTTGCCC61.9230769230769231No Hit
TACTCAAGCATAGATCCAAGGGCAATCTCTT51.6025641025641024No Hit
TCCTTAATTCTTTCATGGTCCATACCGGGT51.6025641025641024No Hit
TCTCATGGTCCATACCGGGTTCTATTCTAAAACAAAGGGTTTGTACA51.6025641025641024No Hit
TTACTGACTCCCATCCCCAAAAAAGGCACC41.282051282051282No Hit
TTAGAAAAGCAACACTGGATTCTCGAACTGT41.282051282051282No Hit
TAGGTGACATGACGTGTGGTCTACGTGG41.282051282051282No Hit
TCAGGACTGGGTACCCGAAATGCTTCATA41.282051282051282No Hit
TTGGATATCGTTGACCCTCCAAAGTCCAC30.9615384615384616No Hit
TTCTCTACTTTCAGGACTGGGTACCCGAA30.9615384615384616No Hit
TTTGCACAGTGAATCCACGTCCACTACCCAG30.9615384615384616No Hit
TACTGACTCCCATCCCCAAAAAAGGCACCA30.9615384615384616No Hit
TGAACATGTTGTCCATATCCTCCACCCCT30.9615384615384616No Hit
TATCATAACCTTGCCCCCAATTGCTCAGT30.9615384615384616No Hit
CCTTGCCCCCAATTGCTCAGTTTGTGCGAG20.641025641025641No Hit
TATTACATCGTCCAATGTGACGTCCCCCCT20.641025641025641No Hit
TATAAAATCCAGTAGAGCTTGAGGGTGAAC20.641025641025641No Hit
ATCCACACCTATCATAACCTTGCCCCCAATTGCTCAGTTTGTGCGAGCAAGCAGAATCTTTGATTAGCTCAACCA20.641025641025641No Hit
TCCACGTCCACTACCCAGCTTTAGTTCACAC20.641025641025641No Hit
TCTCTACTTTCAGGACTGGGTACCCGAAA20.641025641025641No Hit
TCCATATCCTCCACCCCTTCAGGAAGTAT20.641025641025641No Hit
ACAACTTCTCTACTTTCAGGACTGGGTACCC20.641025641025641No Hit
TAATTGGGGGGAGGAGG20.641025641025641No Hit
TCTGAACATGGACATCTGATAGCTCGGCT20.641025641025641No Hit
TTAGACCTTACACGTCACTCACTAGGAGATG20.641025641025641No Hit
GTGTGGAGCTTACAC20.641025641025641No Hit
TTTTGACTCCGTGACCTCGCTATCAGTCA20.641025641025641No Hit
TTTGACAGCTCTCTCTTGAGTAGACATTGATTTCCTTGAGTTAGTCTTCTTCTAAACATTCGGTAATATCTGTTG20.641025641025641No Hit
TAACATTACTGCCCTTAAGAGTTCTTCCG20.641025641025641No Hit
TAGGTGACATGACGTGTTGTCTACGTGGA20.641025641025641No Hit
TCCTTAATTCTCTCATGGTCCATACCGGGTT20.641025641025641No Hit
TAGGTGACATGACGTGTTGTCTACG20.641025641025641No Hit
TAAGGCTCCATGGTTCTTAAACTACGAACG20.641025641025641No Hit
TGAACATGGACATCTGATAGCTCGGCTGCT20.641025641025641No Hit
TCGAACTGTATGTTCCAAATTTGGCAAGGA20.641025641025641No Hit
TTGTGCTCTCCTTGACTGTCG20.641025641025641No Hit
TGTATGTTCCAAATTTGGCAAGGACTCAAT10.3205128205128205No Hit
TGTGACGTCCCCCCTTAACATTACTGCCCTT10.3205128205128205No Hit
TCTCCTTGACTGTCGTATTTATTCCCATCT10.3205128205128205No Hit
TAGAGTTCAGCAAATCATTAGAGTCAGGC10.3205128205128205No Hit
GGACTCCTCTGCATA10.3205128205128205No Hit
TATAAAATCCAGGAGAGCTTGAGGGTGAAC10.3205128205128205No Hit
TTATGGTTAGAATCGTAGTCTGGGGGGA10.3205128205128205No Hit
TCTCTACTTTCAGGACTGGGTACCCGAA10.3205128205128205No Hit
TCATAACCCCAAGAATCTCCAGATAGATC10.3205128205128205No Hit
GCCTGAACATGTTGTCCATATCCTCCACCC10.3205128205128205No Hit
TGTGACGTCCCCCCTTAACATTACTGCCCTTAAGAGTTCT10.3205128205128205No Hit
TCAGGACTGGGTACCCGAAATGCTTCATAAC10.3205128205128205No Hit
TCCCCCCTTAACATTACTGCCCTTAAGAGT10.3205128205128205No Hit
TGTGACGTCCCCCCTTAACATTACTGCCCTTAAGAGTTCTTCCGGTAGGCCATCCTTCCTT10.3205128205128205No Hit
TAATTCTCTCATGGTCCATACCGGGTTCTATTCTAAAACAAAGGGTTTGTACATCTTGAA10.3205128205128205No Hit
TTAAATCAGGATCTCTACTTTTTATCAACCT10.3205128205128205No Hit
TCAGACAAAATTCTCTCTTAACTACACCCAT10.3205128205128205No Hit
GCATTACTCGTGTTTCCTCCTTCCCTCCGT10.3205128205128205No Hit
TACTCGTGTTTCCTCCTTCCCTCCGTAAT10.3205128205128205No Hit
TGATAGCTCGGCTGCTAACCTAATAGCACC10.3205128205128205No Hit
TCCCCCCTTAACATTACTGCC10.3205128205128205No Hit
TGGTTTATGGTTAGAATCGTAGTCTGGG10.3205128205128205No Hit
TTCGTCTTCCATGATTCGTCTTCCATGTT10.3205128205128205No Hit
TAAAATCCAGTAGAGCTTGAGGGTGAACC10.3205128205128205No Hit
TCATCATAATCGCTCCCTTCTCGTCCTTGT10.3205128205128205No Hit
AGAGCTTTTACCCCAACCGATTTCTCTAGC10.3205128205128205No Hit
TCATAACCTTGCCCCCAATTGCTCAGTT10.3205128205128205No Hit
TGTCTAATCTCGCCAGTCCCTCTGTCATA10.3205128205128205No Hit
TAGGTCCTCCTGTTCTAACTAACTTTGTGCT10.3205128205128205No Hit
TCCTTGACTGTCGTTTTTATTCCCATCTT10.3205128205128205No Hit
TCTTACACGCTCTCTTAGTACCCTGGCTTA10.3205128205128205No Hit
ATCCACACCTATCATAACCTTGCCCCCAATTGCTCAGTTTGTGCGAGCAAGCAGAAT10.3205128205128205No Hit
TCGTTGACACCTGTTCCATACTGTTTTAAAGGGTCTAACAACTTCTCTACTTTCAGGACTGGGTACCCGAAATGC10.3205128205128205No Hit
TAATTCTTTCATGGTCCATACCGGGTTC10.3205128205128205No Hit
TCCCAGTTGAATTACTGACTCCCATCCCCAAAAAAGGCACCATTTTTTAAGATC10.3205128205128205No Hit
TCTAATAATCTTTTTGACTCCGTGACCTCGC10.3205128205128205No Hit
TGTCATAGTGTTGAGGGTGCTGATCATAG10.3205128205128205No Hit
TTTGACTCCGTGACCTCGCTATCAGTCATAG10.3205128205128205No Hit
TACTCGTGTTTCCTCCTTCCCTCCGTA10.3205128205128205No Hit
TCATAACCTTGCCCCCAATTGCTCAGTTT10.3205128205128205No Hit
TGCTTCATAACCCCAAGAATCTCCAGA10.3205128205128205No Hit
TTCGTCTTCCATGTTTAGGTCCTCCTGTT10.3205128205128205No Hit
TGTGACGTCCCCCCTTAACATTACTACCCT10.3205128205128205No Hit
TAATTGGGGGGAGGAGGCTTATCATAAAGC10.3205128205128205No Hit
TTCTAAAACAAAGGGTTTGTATATCTTGAATGAAGTCCCAGAGGTGACTT10.3205128205128205No Hit
ATCATATTGATGGTGTTAGTGTCCACCGGTTCGTTGACACCTGTTCCATACTGTTTTAAAGGGTCTAACAACTTC10.3205128205128205No Hit
AACATTACTGCCCTTAAGAGTTCTTCCGG10.3205128205128205No Hit
TGCTCCTCTGTGCACTGGTCTACAGGAATT10.3205128205128205No Hit
TGGGGGGAGGA10.3205128205128205No Hit
TTAATTCTCTCATGGTCCATACCGGGTT10.3205128205128205No Hit
TTCTCTCATGGTCCATACCGGGTTCTATTCT10.3205128205128205No Hit
TCCGGTAGGCCATCCAATAAATATTCCT10.3205128205128205No Hit
TAGAGCATACGGATAGCAGGATTGGTTAC10.3205128205128205No Hit
TTAGGGTGGTTATCACTAGATGATGTGTT10.3205128205128205No Hit
TGCTTCATAACCCCAAGAATCTCCAGATAGATC10.3205128205128205No Hit
TGCCTGAACATGTTGTCCATATCCTCCACC10.3205128205128205No Hit
TGTATATCTTGAATGAAGTCCCAGAGGT10.3205128205128205No Hit
TTATTACATCGTCCAATGTGACGTCCCCCCTTAACATTACTGCCCTTAAGAGTTCTTCCGGTAGGCCATCCTTCC10.3205128205128205No Hit
TCCTAAGTTGAGGAATGCCTTGATGTGCCC10.3205128205128205No Hit
TTACATCGTCCAATGTGACGTCCCCCCTTAACATTACTGCCCTTAAGAGTTCTTCCGGTAGGCCATCCT10.3205128205128205No Hit
TGAATTACTGACTCCCATCCCCAAAAAAGG10.3205128205128205No Hit
TTCTCTCATGGTCCATACCGGGTTCTATTCTAAAACAAAGGGTTTGTACA10.3205128205128205No Hit
TCTGATAGCTCGGCTGCTAACCTAATAGCACCTAT10.3205128205128205No Hit
TTCTCTACTTTCAGGACTGGGTACCCGAAAT10.3205128205128205No Hit
TTATCTTACACGCTCTCTTAGTACCCTGGCCC10.3205128205128205No Hit
TTTATGGTTAGAATCGTAGTCTGGGGGG10.3205128205128205No Hit
TACTGACTCCCATCCCCAAAAAAGGCACC10.3205128205128205No Hit
GGTGCTTAGGTCCTCCTGTTCTAACTAACTT10.3205128205128205No Hit
TTCTATTCTAAAACAAAGGGTTTGTACATC10.3205128205128205No Hit
TTCAGGACTGGGTACCCGAAATGCTTCATAA10.3205128205128205No Hit
TGCATAGATTGGAGAGCTGTGATGCCCC10.3205128205128205No Hit
TGACTTGCCTGAACATGTTGTCCATATCCT10.3205128205128205No Hit
CCTGCGATTGTTGCAACCTTGTGGTTGTC10.3205128205128205No Hit
ATTACATCGTCCAATGTGACGTCCCCCCT10.3205128205128205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)