FastQCFastQC Report
Mon 16 Nov 2020
S33.raw.3452.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.3452.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220
Sequences flagged as poor quality0
Sequence length12-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCTCGGTACTGACTCGACAAGCGCC12510.245901639344263No Hit
TGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAA584.754098360655737No Hit
TCTATATTGTCGAATAACCCACCTCTTGG504.098360655737705No Hit
TTGGGCTCGGTACTGACTCGACAAGCGCCT433.524590163934426No Hit
TTAACGACCCGCTCTGTTCCATATCGATA332.7049180327868854No Hit
TTTGTTAACTGGCCGACTTCCCCCGCCT312.540983606557377No Hit
TATTGTCGAATAACCCACCTCTTGG292.3770491803278686No Hit
TAACGACCCGCTCTGTTCCATATCGATAT282.2950819672131146No Hit
CACGTCGTGTACGACGCGAGACCGCGGAG201.639344262295082No Hit
TTAACGACCCGCTCTGTTCCATATCGATAT191.557377049180328No Hit
TCGGTACTGACTCGACAAGCGCCTCGTAC181.4754098360655739No Hit
TATACGTCGTGTACGACGCGAGACCGCGGA181.4754098360655739No Hit
TGGGCTCGGTACTGACTCGACAAGCGCCT171.3934426229508197No Hit
TTAGATGGACCCGGTATAGGATTTTCGCTC161.3114754098360655No Hit
TAAAAGGACCGATTCGGGCAGTGAGTGTC161.3114754098360655No Hit
TAACACAAGCCCGATCGCTCCGATACATG151.2295081967213115No Hit
TGAATTGCCGTGGCTCGGAAGTTGGGCTC141.1475409836065573No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGAC131.0655737704918031No Hit
TGAAAGTGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAA131.0655737704918031No Hit
TCGGTACTGACTCGACAAGCGCCTCGTACT131.0655737704918031No Hit
TTCTTGTTCGGAAAAGTCGCTGGCTTCGT100.819672131147541No Hit
AAAAGTCACCGTCGTCCTCATGACTGCCT100.819672131147541No Hit
TGGGCTCGGTACTGACTCGACAAGCGCC90.7377049180327869No Hit
TACAATTTCTGCATCGTTATAGCACTGAT80.6557377049180327No Hit
TCTACATTGTCGAATAACCCACCTCTTGGT80.6557377049180327No Hit
TACTGACTCGACAAGCGCCTCGTACTTGT70.5737704918032787No Hit
TCATCTTCTTGTTCGGAAAAGTCGCTGGCT70.5737704918032787No Hit
TATTGTCGAATAACCCACCTCTTGGTG70.5737704918032787No Hit
TACTTGGATTTGTGGGATGATGGCATCGC60.49180327868852464No Hit
TCAAAACTCCCTCTTCGAACGTAACACAAG60.49180327868852464No Hit
TCTATATTGTCGAATAACCCACCTCTTGGT60.49180327868852464No Hit
AAGTGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAA50.4098360655737705No Hit
TGCCGAAGCCATAAAAGGACCGATTCGGGC50.4098360655737705No Hit
TAGATGGACCCGGTATAGGATTTTCGCTC50.4098360655737705No Hit
TATTGTCGAATAACCCACCTCTTGGT50.4098360655737705No Hit
TAGGGAATTTTTGAATGGCTGCTATTTT50.4098360655737705No Hit
GTGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAA50.4098360655737705No Hit
CACGACGCATTTTCGTAGTTCTCCCCAAGC50.4098360655737705No Hit
TCTGAGAAAAAGTCACCGTCGTCCTCATGAC40.32786885245901637No Hit
TTAGATGGACCCGGTATAGGATTTTCGCT40.32786885245901637No Hit
TTCCAACGTCTCGTTCCATCGGATC40.32786885245901637No Hit
TAGACGTTAACGGCGCTGAGGGGTGTTG40.32786885245901637No Hit
CGCGAGACCGCGGAGGCACTCTTTGAAGG40.32786885245901637No Hit
TATTCACTGGCTTCCGCTTCAAAACTCCT40.32786885245901637No Hit
TTGGGCTCGGTACTGACTCGACAAGCGCCC40.32786885245901637No Hit
TCACTGGCTTCCGCTTCAAAACTCCTCTC40.32786885245901637No Hit
TCCCAAACTTCGACTCGGACCAGTGCCGG40.32786885245901637No Hit
TCGGAAAAGTCGCTGGCTTCGTATTCACT40.32786885245901637No Hit
TATTTTTTTTGGGCGGATTCTTTCGGGG40.32786885245901637No Hit
TGAATTGCCGTGGCTCGGAAGTTGGGCTCT40.32786885245901637No Hit
TCCGAATCGATATTGTCGAATAACCCACCT40.32786885245901637No Hit
TTTGTTAACTGGCCGACTTCCCCCGCCTGA30.24590163934426232No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGCC30.24590163934426232No Hit
ATCTATATTGTCGAATAACCCACCTCTTG30.24590163934426232No Hit
TTGAATTGCCGTGGCTCGGAAGTTGGGCTC30.24590163934426232No Hit
TCGAGAAATTGGGACACTGTAATATTGGGT30.24590163934426232No Hit
TACACGTCGTGTACGACGCGAGACCGCGGA30.24590163934426232No Hit
TTTACGGTAGTAGCCTAGTAATCCGAGAAA30.24590163934426232No Hit
TGAGGACCAGGGGCTGTGTGAGGGCCTAA30.24590163934426232No Hit
TTGGGGATCCCGTAAGAAATACACGTCGTG30.24590163934426232No Hit
TTGGGCTCGGTACTGACTCGACAAGCGCCTCGTACTTGTCGATTAAC30.24590163934426232No Hit
TTGATTCCTCATTGACTTCAATTTGATGAAAGCCTGACGCAAGGTCTAGG30.24590163934426232No Hit
TATATTGTCGAATAACCCACCTCTTGG30.24590163934426232No Hit
TACTGACTCGACAAGCGCCTCGTACT30.24590163934426232No Hit
TAACGACCCGCTCTGTTCCATATCGATATT30.24590163934426232No Hit
TTTTCGCCGCTGCAGCCGAATACGGTAGGTACGCCCTCCGATTTGAACGTACATAACTTTTT30.24590163934426232No Hit
TTTGTCCGGAGGCATCTATTTTTTTGGGA30.24590163934426232No Hit
TTAACGACCCGCTCTGTTCCATATCGATATTGTCGAATAACCCACCTCTTGGTG30.24590163934426232No Hit
TAACGACCCGCTCTGTTCCATATCGATA30.24590163934426232No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGC30.24590163934426232No Hit
CCCCGCCTGAAAGTGGTCCATTAAGGCCTTGTTGTAGACC30.24590163934426232No Hit
TTACGGTAGTAGCCTAGTAATCCGAGAAATGATTTCATCTGTGTTTGTGTTCGTGGAATAGGGAAT30.24590163934426232No Hit
TAACAGGAGTACGGCCCACAGTAGCGTC30.24590163934426232No Hit
TATCTCGATTAATTAAAACTGCACAGACGCGGTGTTTACTCTCTGAAAA30.24590163934426232No Hit
TTGTTAACTGGCCGACTTCCCCCGCCT30.24590163934426232No Hit
TTCTACTTGGATTTGTGGGATGATGGCATC30.24590163934426232No Hit
TCTTCTTGTTCGGAAAAGTCGCTGGCTTCGT30.24590163934426232No Hit
TCGGTAGTTAATTCGAAGGGTTTCGAGAA30.24590163934426232No Hit
TGATGGCAGGAGATTGAACGTTTGTTTCG30.24590163934426232No Hit
TACTGGAAGTGCGGAGAACTTTAGTCCCA30.24590163934426232No Hit
TGCAATATAGACGTTAACGGCGCTGAGGG20.16393442622950818No Hit
TCGATTAACGAACTCCACACTTCTTTGGG20.16393442622950818No Hit
TGCACAGACGCGGTGTTTAGTCTCTGAAA20.16393442622950818No Hit
GGATGAAGACGCAGCGGCATGTTGAACTG20.16393442622950818No Hit
TTGGGCGGATTCTTTCGGGGGTGACAATA20.16393442622950818No Hit
TTTGTTGTAACATTTTTTGTATTTGATTGT20.16393442622950818No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGA20.16393442622950818No Hit
TCACTGGCTTCCGCTTCAAAACTCCTCT20.16393442622950818No Hit
TCGATATTGTCGAATAACCCACCTCTTGG20.16393442622950818No Hit
TTAGAGTTTCAAATGCTGTTGGTTCTGCT20.16393442622950818No Hit
TACATTGTCGAATAACCCACCTCTTGGT20.16393442622950818No Hit
ACGTCTTGCTTTCGACGCGCTAGAGTGGCTGCATGAAGGAAGATTGATGGCAGGAGATTGAACGTTTGTTTCGG20.16393442622950818No Hit
TTAACGACCCGCTCTGTTCCATATCGATAA20.16393442622950818No Hit
TCTTGCTTTCGACGCGCTAGAGTGGCTGC20.16393442622950818No Hit
TTCGGTGTCCACACGAGAAACAGAAACGC20.16393442622950818No Hit
ACCACCCAAAGAGATGAGGACCAGGGGCTGT20.16393442622950818No Hit
TAAGAAATACACGTCGTGTACGACGCGAGACCGCGGAGGCACTC20.16393442622950818No Hit
TCAAAACTCCCTCTTCGAACGTAACACAAGC20.16393442622950818No Hit
TCCCCAGGGGAGCGGCGAATATTAAGTTTTCGTGGTTAAG20.16393442622950818No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGC20.16393442622950818No Hit
TAAAAGGACCGATTCGGGCAGTGAGTGTCT20.16393442622950818No Hit
TCGTGTACGACGCGAGACCGCGGAGGCACTCTTTGAAGGAGTGCCAC20.16393442622950818No Hit
TCGTGGATGACGTGGACGTGGTCGTTGTG20.16393442622950818No Hit
TTAGACAGAATAATAACAGGAGTACGGCC20.16393442622950818No Hit
TGCCTCCTGCCACCAATCCTTCTTGTTTT20.16393442622950818No Hit
TTGTTCGGAAAAGTCGCTGGCTTCGTATT20.16393442622950818No Hit
TGCCACGACGCATTTTCGTAGTTCTCCCC20.16393442622950818No Hit
CATTGTCGAATAACCCACCTCTTGGT20.16393442622950818No Hit
TCCGGCGCGCTAGAGTGGCTGCATGAAGGAA20.16393442622950818No Hit
TTGGTATGATCACCTCTGAGTATGGGGCT20.16393442622950818No Hit
CTTGTTCGGAAAAGTCGCTGGCTTCGTATT20.16393442622950818No Hit
TCAAAACTCCCTCTTCGAACGTAACACAAGCCCGATCGCTCCGATACATG20.16393442622950818No Hit
TGGCTTCCGCTTCAAAACTCCTCTCGAT20.16393442622950818No Hit
TATAGACGTTAACGGCGCTGAGGGGTGTT20.16393442622950818No Hit
TCAAAACTCCTCTCGATTTGCGGGGAGGAGGACTCAAAACTCCCTCTTCGAACGTAACACAAGCCCGATCGCT20.16393442622950818No Hit
TTAACGACCCGCTCTGTTCCATATCGATATT20.16393442622950818No Hit
TCACACACACTACTGATACTATCGGTCTCA20.16393442622950818No Hit
TTCTTGTTCGGAAAAGTCGCTGGCTTCGTAT20.16393442622950818No Hit
TATAGACGTTAACGGCGCTGAGGGGTGT20.16393442622950818No Hit
GGGGTTGTTATCGATAGTTGGAATGTTGTGTTTTACGATTGATGAGAATGTTAAATC20.16393442622950818No Hit
TATACGTCGTGTACGACGCGAGACCGCGGAC20.16393442622950818No Hit
TATACGTCGTGTACGACGCGAGACCGCGGAA20.16393442622950818No Hit
TTTTGTTAACTGGCCGACTTCCCCCGCCT20.16393442622950818No Hit
TCATGACTGCCTCCTGCCACCAATCCTTC20.16393442622950818No Hit
TTAGATGGACCCGGTATAGGATTTTCGGTC20.16393442622950818No Hit
TATTAATGAGACCGCGGAGGACTATCTC20.16393442622950818No Hit
TTCGACTGTTAGGTGGGCAAAATTTGGGA20.16393442622950818No Hit
CGGAAATTGGGCTCGGTACTGACTCGACAAGCGCCTCGTACTTGTCGATTA20.16393442622950818No Hit
TTCAAATGCTGTTGGTTCTGCTTGCGGCTC20.16393442622950818No Hit
TTCTAACAAACTGACGGACACAAACCCTG20.16393442622950818No Hit
TTAACGACCCGCTCTGTTCCATATCGAT20.16393442622950818No Hit
AACGACCCGCTCTGTTCCATATCGATATTGTC20.16393442622950818No Hit
TGCGGCTCATTCCAAAGAAGAATTCTTTT20.16393442622950818No Hit
TCCTCATGACTGCCTCCTGCCACCAATCC20.16393442622950818No Hit
TGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCA20.16393442622950818No Hit
TATCGCCTATTGTTTTTTCGTTTAGTTTC20.16393442622950818No Hit
AAAGTGGTCCATTAAGGCCTTGTTGTAGACCTCTGTCGCACGGCCCAA20.16393442622950818No Hit
TACACGTCGTGTACGACGCGAGACCGCGGAGGCACTCTT20.16393442622950818No Hit
CTCCCTCTTCGAACGTAACACAAGCCCGATC20.16393442622950818No Hit
TTACGGTAGTAGCCTAGTAATCCGAGAAAT20.16393442622950818No Hit
TCTTGCTTTCGACGCGCTAGAGTGGCTG20.16393442622950818No Hit
TCGGATCCTATTGCATATGCGGATGCATC20.16393442622950818No Hit
TGCGAGACCGCGGAGGCACTCTTTGAAGCAG20.16393442622950818No Hit
TCGTACTTGTCGATTAACGAACTCCACACT20.16393442622950818No Hit
TTATTAATGAGACCGCGGAGGACTATCTC20.16393442622950818No Hit
TTCGAGAAATTGGGACACTGTAATATTGGG20.16393442622950818No Hit
TCGTCCTCATGACTGCCTCCTGCCACCAATC20.16393442622950818No Hit
TATATTGTCGAATAACCCACCTCTTGGT20.16393442622950818No Hit
TTAATTCGAAGGGTTTCGAGAAATTGGGAC20.16393442622950818No Hit
TCCCTCTTCGAACGTAACACAAGCCCGATC20.16393442622950818No Hit
TAACACAAGCCCGATCGCTCCGATACATGG20.16393442622950818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)