FastQCFastQC Report
Mon 16 Nov 2020
S33.raw.5721.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.5721.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137
Sequences flagged as poor quality0
Sequence length11-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGCGGGCAGAGGGAGAGCTGGAGGACAGATGTTGGACCCCT64.37956204379562No Hit
TTAAATTGCAGAATTGACGGCCCACCAAAC53.64963503649635No Hit
AGTGTGCCAGCTCAAGCAGGAAATAGAGTTGCTGCGTAACGAGCTGCAGCGGGCAGAGGGAGAGCTGGAGGAC42.9197080291970803No Hit
TTTAAATTGCAGAATTGACGGCCCACCAAAC42.9197080291970803No Hit
TACAAACGATCTCCTGAGCGCTGGAACTGA32.18978102189781No Hit
TTAGGTGTGGTTCACATTCAACATCGGCCCTCACAATTTAGCCACC32.18978102189781No Hit
CAACCATGGCCTATTATTATTACCCTACTGAACAACTCAAAAATCTAAGA32.18978102189781No Hit
TTAAATTGCAGAATTGACGGCCCACCAAACATCAAAGAGA32.18978102189781No Hit
AGGAGCTGGAACGCGCCCGACAGGAGCAGGAGACGGC32.18978102189781No Hit
TTATGACGGCCCACCAAACATCAAAGAGA32.18978102189781No Hit
AGGATGGAATTTTGGCCGGTAGCGCTGCGCCGGACGGACGCGCGCCCA32.18978102189781No Hit
TTGCAGAATTGACGGCCCACCAAACATC32.18978102189781No Hit
TATGACGGCCCACCAAACATCAAAGAGAA32.18978102189781No Hit
TCATTTAAATTGCAGAATTGACGGCCCACCAAACATCAAAGAGA21.4598540145985401No Hit
AAAATCGGAAAAAAACTTGGCGTTTAGGTGTGGTTCACATTCAACATCGGCCCTCACAATTTAGCC21.4598540145985401No Hit
GTAACGAGCTGCAGCGGGCAGAGGGAGAGCTGGA21.4598540145985401No Hit
TGAAGAAATTACAAACGATCTCCTGAGCGT21.4598540145985401No Hit
GATTTAAATTGCAGAATTGACGGCCCACCAAACATCAAAGAGA21.4598540145985401No Hit
TTGCTGCGTAACGAGCTGCAGCGGGCAGAGGGAGAGCTGGAGGACAG21.4598540145985401No Hit
CAAAAATATAAGACTCCAAACAGCAAAAACAAACCTAGACATCATGACACAAGAAAAG21.4598540145985401No Hit
ACGTGGAAGTGTGCCAGCTCAAGCAGGAAATAGAGTTGCTGCGTAACGAGCTGCAGCGGGCAGAGGGAGAGC21.4598540145985401No Hit
TCATTTAAATTGCAGAATTGACGGCCCACC21.4598540145985401No Hit
TCGGAAAAAAACTTGGCGTTTAGGTGTGGT21.4598540145985401No Hit
ATAAGACTCCAAACAGCAAAAACAAACCTAGACATCATGACACGAGAA21.4598540145985401No Hit
TTTAAATTGCAGAATTGACGGCCCACAAAAC21.4598540145985401No Hit
TGAAAGGGTTGGCAGTTGGAAATGTAAC21.4598540145985401No Hit
TTGGCAGTTGGAAATGTAACGTCTGATTCGG21.4598540145985401No Hit
TGCGCCGGACGGACGCGCGCCCACCAAATTAATTTAACTTTCACGTTATAAATA21.4598540145985401No Hit
GCATTTTGATTTGAACGCCCATTTTCATAATAAGTCTGGATAACTTTAACACGTTGTTGGACTGTGT21.4598540145985401No Hit
TTCACTGACGTGGAAGTGTGCCAGCTCAAGCAGGAAATAGAGTTGCTTCGTAACGAGCTGCAGCGGGCAG21.4598540145985401No Hit
TGACGGCCCACCAAACATCAAAGAGAAATT21.4598540145985401No Hit
TCGGAAAAAAACTTGGCGTTTAGGTGTGG21.4598540145985401No Hit
TTGGAAATGTAACGTCTGATTCGGACTCATCTTACTCAATTCCTCAAATGACGTCTAGCGTCGGTCCT21.4598540145985401No Hit
AGCATCAGGCGCTGCAGAGTGACGGCGCCAACGACAAGTCCGGCGCTGCCT21.4598540145985401No Hit
GCGTAACGAGCTGCAGCGGGCAGAGGGAGAGCTGGAGGACAGATGTTGGA21.4598540145985401No Hit
GATTTAAATTGCAGAATTGACGGCCCACC10.7299270072992701No Hit
CCATGTATCATAGCTGGAGATTTAAATTGCAGAATTGACGGCCCACCAAA10.7299270072992701No Hit
TGTCAAAAAAAAAATCGGAAAAAAACTTGGC10.7299270072992701No Hit
GGTTCTGGGCC10.7299270072992701No Hit
ACATTCAACATCGGCCCTCACAATTTAGCCACCCTTTATATCAGA10.7299270072992701No Hit
TTTGGCCGGTAGCGCTGCGCCGGACGGACGCGCGCCC10.7299270072992701No Hit
ATTATTACCCTACTGAACAACCCAAAAATATAAGACTCCAAACAGCAAAA10.7299270072992701No Hit
CGTTGAAGAAATTACAAACGATCTCCTGAGCGTTGGAACCGAACCATGTATTA10.7299270072992701No Hit
TACCCTACTGAACAACTCAAAAATCTAAGACTCCAAACAGCAAAAACAAACCTAGACATCATG10.7299270072992701No Hit
CGGCCCACCAAACATCAAAGAGA10.7299270072992701No Hit
TATGACGGCCCACCAAACATCAAAGAGAAATT10.7299270072992701No Hit
ACCCAAAAGAAAAGGATATCACAGACATCGTTGAAGAAATTACAAACGATCTCCTGAGAGTTGGAACTGAACCA10.7299270072992701No Hit
TTACAAACGATCTCCTGAGCGCTGGAACT10.7299270072992701No Hit
GTTTTCTTGGCTGTAC10.7299270072992701No Hit
TTCTACAGACCCGGTTTGCTCGAATTTTTCCACGATTTTTGCGATTGTACGCACATTTG10.7299270072992701No Hit
GACGCGCGCCC10.7299270072992701No Hit
CGAGCCTTGGGATAAACAGAATCGAGCAAAATAAACCCAAAAGAAAATGATATCACAGACATCGTTGAAGAA10.7299270072992701No Hit
ACGCACATTTGGACGATGCATATCGTGCACGAAGTGTACGATATGCATTT10.7299270072992701No Hit
AACGTCTGATTCGGACTCATCTTACTCAATTCCTCAAATGACGTCTAGCGTCGGTCCTTGTGTACT10.7299270072992701No Hit
TAAATTGCAGAATTGACGGCCCACCAAAC10.7299270072992701No Hit
TTTATTTTTATT10.7299270072992701No Hit
TTTGGCAGTTGGAAATGTAACGTCTGATT10.7299270072992701No Hit
GAAATTACAAACGATCTCCTGAGAGTTGGAACTGAACCATGTATC10.7299270072992701No Hit
AAAAGATAAAGAGCCTTGGAATAAACAGAATCGAGCAGAATAAAC10.7299270072992701No Hit
TTGGCCGGTAGCGCTGCGCCGGACGGACGCGC10.7299270072992701No Hit
TTGGTACTGAAAGGGTTGGCAGTTGGAAATGTAACGTCTGATTC10.7299270072992701No Hit
ATTGACGGCCCACCAAACATCAAAGA10.7299270072992701No Hit
TTATTATTACCCTACTGAACAACTCAAAAAT10.7299270072992701No Hit
TCCTCAAATGACGTCTAGCGTCGGTCCTTG10.7299270072992701No Hit
CTACTGAACAACTCAAAAATATAAGACTCCAAACAGCAAAAACAAACCGAGACATCATGAC10.7299270072992701No Hit
AAATTGCAGAATTGACGGCCCACCAAAC10.7299270072992701No Hit
AACAAACCTAGACATCATGACACGAGAAAAGATAAAGAGCCTTGGAATAAACAGAATCGAGCAG10.7299270072992701No Hit
TTTGATTTGAACGCCCATTTTCATAATAAGTCTGGATAACTTTAACACGTTGTTGGATTGTGTATCTCTCT10.7299270072992701No Hit
TTTAAATTGCAGAATTGACGGCCCACCAAACATCAAAGAG10.7299270072992701No Hit
AAAAAAAATCGGAAAAAAACTTGGCGTTTAGGTGTGGT10.7299270072992701No Hit
GAACTGAACCATGTATCATAGCTGGAGATT10.7299270072992701No Hit
TTAGCCACCCTTTATTACCCCATTGCTTGTGAGAGT10.7299270072992701No Hit
TCCTACAGATTTTGGCCGGTAGCGCTGCGCCGGACGGACGCGCGCCCACC10.7299270072992701No Hit
AGAAGGAGCTGGAACGCGCCCGACAGGAGCAGGAGACGGCGGCGTCGGAG10.7299270072992701No Hit
GCCTCCCCCGGC10.7299270072992701No Hit
GACCCGGTTTGCTCGAAATTTTCCACGATTTTTGCGATTGTACGCACATTTGGACGATGCATATCGTGCACGAAG10.7299270072992701No Hit
TCACAGACATCGTTGAAGAAATTACAAACG10.7299270072992701No Hit
AAGACTCCAAACAGCAAAAACAAACCTAGACATCATGACACGAGAAAAGATAAAGAGCCT10.7299270072992701No Hit
ATTCGGAAAAAAACTTGGCGTTTAGGTGTGGTTCACATTCAACATC10.7299270072992701No Hit
TGTTGCAGGTTGAACACAAAGAGATCCGAGTTTTCTTTAGCCAGTAGACT10.7299270072992701No Hit
TGCTGATGGTGAACAATGACGGGTTCTGG10.7299270072992701No Hit
TGAGCGTTGGAACTGAACCATGTATCATAGCTGGAGATTTAAATTGCAGA10.7299270072992701No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)