FastQCFastQC Report
Mon 16 Nov 2020
S34.raw.12121.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.12121.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290
Sequences flagged as poor quality0
Sequence length11-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTAACACAAGAATAAGAGACAAGCCT7425.517241379310345No Hit
TACTAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATGGA279.310344827586208No Hit
ATGTATACTAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATGGA206.896551724137931No Hit
CGGGAGAGGGCC186.206896551724138No Hit
GGGAGAGGGCC113.793103448275862No Hit
TTAGATCAAAAC93.103448275862069No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCCCTAACCCACTTAATTACTTTCC62.0689655172413794No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCCCTAACCCACTTAATTACTTTCCAGG51.7241379310344827No Hit
ATAATATTTACTGTCAATAAAGAGTT41.3793103448275863No Hit
AACAACGCCGGAG41.3793103448275863No Hit
TTGTATTCGCTTCCGTGTCTGTTCGTTC41.3793103448275863No Hit
ACCAGAGGACTT41.3793103448275863No Hit
TACTAACACAAGAATAAGAGACAAGCCTCCAC31.0344827586206897No Hit
TGTATACTAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATGGA31.0344827586206897No Hit
TACTAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATGGATGA31.0344827586206897No Hit
AAGGAAAGGGACGGGCTATTGTATTCGCTTCCGTGTCGGTTC31.0344827586206897No Hit
AAGGAAAGGGACGGGCTATTGTATTCGCTT31.0344827586206897No Hit
TTTACTGTCAATAAAGA20.6896551724137931No Hit
TGACTCCACCACAAGAAGAAGAGACAAGC20.6896551724137931No Hit
GAAGAAGAGACAAGCCCTAACCCACTTAATTACTTTCC20.6896551724137931No Hit
AACACAAGAATAAGAGACAAGCCTCCACC20.6896551724137931No Hit
TTAGATCAAAACCC20.6896551724137931No Hit
AAAAAAAAAAAA20.6896551724137931No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCCCTAACCCACTTAATTACTTTCCAG20.6896551724137931No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCTCTAACCCACTTAATTACTTTCCAGG20.6896551724137931No Hit
GACTCCACCACAAGAAGAAGAGACAAGCCCTAACCCACTTAATTACTTTCCAGG20.6896551724137931No Hit
AAGGAAAGGGACGGGCT20.6896551724137931No Hit
TAACACAAGAATAAGAGACAAGCCTCCAC20.6896551724137931No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCCCTAACCC20.6896551724137931No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCCCTAACCCACTTAATTACTTTCCAGGG20.6896551724137931No Hit
GTTATAAAAAGGTAAGGGACGGGCTATTGTATTCGCTTCCGTGTC20.6896551724137931No Hit
TATACTAACACAAGAATAAGAGACAAGC20.6896551724137931No Hit
ATTGAAATCGAATT20.6896551724137931No Hit
CTTTGATTGTATAATATTTACTGTC20.6896551724137931No Hit
TACTAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATGGATGACTC20.6896551724137931No Hit
AACACTGGCTGC20.6896551724137931No Hit
TTAGCCCGTCCCTATTTATAACTTCAAGGA20.6896551724137931No Hit
TACTAACACAAGAATAAGAGACAAGCCA10.3448275862068966No Hit
TATACTAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATGGA10.3448275862068966No Hit
ATAAGGAAAGGGACGG10.3448275862068966No Hit
TGGGAGAGGGCC10.3448275862068966No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCTCTAACCCACTTAAGTACTTTCC10.3448275862068966No Hit
AAAAAGGAAAGGGACGGGCTA10.3448275862068966No Hit
GTGACGGGCTATTGTATTCGCTTC10.3448275862068966No Hit
TTTTCGTGTAC10.3448275862068966No Hit
AAAGGAAAGGGACGGGCTATTGTATTCGCTT10.3448275862068966No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCCCTAACC10.3448275862068966No Hit
ATGTATACTAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATG10.3448275862068966No Hit
TTCTTTTCTTGTT10.3448275862068966No Hit
ACCCTGGGCTG10.3448275862068966No Hit
TATTTATTGTA10.3448275862068966No Hit
TGCTTTTTAGCCCGTCCCTATTTATAACTT10.3448275862068966No Hit
ATAAAAAGGAAAGGGACGGGCT10.3448275862068966No Hit
ATGACTCCACCACAAGAAGAAGAGACAAGCCCTAACC10.3448275862068966No Hit
ATTAGATCAAAACC10.3448275862068966No Hit
ACAAGAAGAAGAGACAAGC10.3448275862068966No Hit
ATGGGAGAGGGCC10.3448275862068966No Hit
TTCGCCCTTTGATTGTATAATATTTACTGT10.3448275862068966No Hit
AAAAAAAAAAAAA10.3448275862068966No Hit
AAGGGTTGAATGGTAGAGATTGCCTAAGGCAAT10.3448275862068966No Hit
GACAGGTTGGC10.3448275862068966No Hit
TACTAACACAAGAATAAGAGACAAGCCTCCA10.3448275862068966No Hit
AAGTTATAAAAAGGAAAGGGACGGGCTATTGT10.3448275862068966No Hit
AAAGAAAGGAATGACAACGCCGGCGCC10.3448275862068966No Hit
TATACTAACACAAGAATAAGAGACAAGCC10.3448275862068966No Hit
TTGTTTCAGTTG10.3448275862068966No Hit
TTTCGCCCTTTGATTGTATAATATTTACTGT10.3448275862068966No Hit
AGAAGAGACAAGCTCTAACCCACTTAATTACTTTCCAGG10.3448275862068966No Hit
ACAAGAAGAAGAGACAAGCCCTAACCCT10.3448275862068966No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCTCTAACCC10.3448275862068966No Hit
AAAAGGAAAGGGACGGGCT10.3448275862068966No Hit
TGACTCCACCACAAGAAGAAGAGACAAG10.3448275862068966No Hit
CTGCTTAGGACC10.3448275862068966No Hit
TAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATGGATGACTCCACCACAAGAAGAAGAGACAAGCTC10.3448275862068966No Hit
TAATGTGTTTGTCCTAGTCCACC10.3448275862068966No Hit
ATGTATACTAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCAGATGTA10.3448275862068966No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCCCT10.3448275862068966No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCCCTAACCCACTTAATTACTTTCCA10.3448275862068966No Hit
TCTCTTCTCCT10.3448275862068966No Hit
TAAAAAGGAAAGGGACGGGCTATTGTATT10.3448275862068966No Hit
TACTAACACAAGAATAAGAGACAAGCCTCCACC10.3448275862068966No Hit
AGTTATAAAAAGGAAAGGGACGGGCTATT10.3448275862068966No Hit
TCGCCCTTTGATTGTATAATATTTACTGTC10.3448275862068966No Hit
TATTAGATCAAAACC10.3448275862068966No Hit
ATTGTATAATATTTACTG10.3448275862068966No Hit
TACTAACACAAGAATAAGAGACAAGCCTCCACCACAAGTCA10.3448275862068966No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)