FastQCFastQC Report
Mon 16 Nov 2020
S34.raw.16656.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.16656.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length11-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTACGATGTG315.789473684210526No Hit
CATACTATCACCGAGGTCAAGGATAATGT210.526315789473683No Hit
TGCGAGCTCCG15.263157894736842No Hit
TTTATTTTTATT15.263157894736842No Hit
AACTTGCTTACT15.263157894736842No Hit
CGTCTCCCGGTGTGGTGTACTAACTGATAGTCTGCAAGATGCTGGAGCAT15.263157894736842No Hit
CTCTAGTCTGAC15.263157894736842No Hit
CGTCCTTGGATC15.263157894736842No Hit
ATGCAGGACACTTGTGACCACACGAG15.263157894736842No Hit
CACAGCCAGGGGA15.263157894736842No Hit
TGAGCTCTCGGGTTGTC15.263157894736842No Hit
ATGAGATTAAAGACGACCCCTCTGAGT15.263157894736842No Hit
AGAATTCCAGTTA15.263157894736842No Hit
ACTCTAGTCTGACA15.263157894736842No Hit
CATACTATCACCGAGGTCAAGGATAATGTAA15.263157894736842No Hit
AGGGTGTCGTC15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)