FastQCFastQC Report
Mon 16 Nov 2020
S34.raw.2854.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.2854.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379
Sequences flagged as poor quality0
Sequence length11-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATACGAAACTGCATCGTGGCACTCC205.277044854881266No Hit
AGATACGAAACTGCATCGTGGCACTC184.749340369393139No Hit
AGCTTGGGGACAGATACGAAACTGCATC153.95778364116095No Hit
AGATACGAAACTGCATCGTGGCACTCCT153.95778364116095No Hit
CCGGTTCAGACGATGAACAATTTCCTGGC102.638522427440633No Hit
TCCTGGCCTATCTGTTTCGTGTGGACGC82.1108179419525066No Hit
ATTGCGCCTACACAAGCTCCACATGTCGTTGCGTCTTCATCAGGCGCATA82.1108179419525066No Hit
CCCTTTCACGGC71.8469656992084433No Hit
TTTGTCAGAATT61.58311345646438No Hit
TAACCGACGGCC51.3192612137203166No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCAGAAGGAACGAAACATTACAGATCATCAAAGAGAA41.0554089709762533No Hit
CCTCGACAAACAGTGGAGCTTCTGCCAGC41.0554089709762533No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCAGAAGGAACGAAACATTACAGATCATC41.0554089709762533No Hit
TCCTGGCCTATCTGTTTCGTGTGGACGCC41.0554089709762533No Hit
CAGATCTAGAACACCAGGTCACCCCGAGC41.0554089709762533No Hit
AAGCACAGGATAGAACTCAATGGAGAGGAAATGAAGAGGCCTTCGTCCAGCAGTGGACAGG30.79155672823219No Hit
TTGCGGCGAATTCGGCATTGGGTCGTGC30.79155672823219No Hit
ATCTAGAACACCAGATCACCCCGAGCA30.79155672823219No Hit
GAACAAGAAGGAC30.79155672823219No Hit
AAGCACAGGATAAAACTCAATGGAGAGGAAATTAAGAGGCCTTCGTCCAGCAGTGGACAGG30.79155672823219No Hit
CCAAAAAGCACAGGATAGAACTCAATGGAGAGGAAATGAAGAGGCCTTCGTCCAGTAGTG30.79155672823219No Hit
GAGATGGCTCACAACCCTTGCAGCTGAGAATAGAGA30.79155672823219No Hit
ATCTAGAACACCAGGTCACCCCGAGC30.79155672823219No Hit
TCAGACCTTCCGCAATCCAGTTTGTTTCCT30.79155672823219No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACC30.79155672823219No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCAGAAGGAACGAAACATTACAGATCATCAAAGAGAAT20.5277044854881267No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCAGAAGGAACGAAACATTACAGATCATCAAAGAGAAG20.5277044854881267No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCAGAAGGAACGAAACATTACAGATCATCAAAGAGAAAG20.5277044854881267No Hit
CAATCTAGCACGACGCACACAGGACGTGGAGTTGAAACGTCAGGTCACCA20.5277044854881267No Hit
AAAAAGCACAGGATAGAACTCAATGGAGAG20.5277044854881267No Hit
TGAAGAGGACTTCGTCCAGCAGTGGACAG20.5277044854881267No Hit
GAACAAGAAGGACTGGTGGCAAGAAGCCA20.5277044854881267No Hit
AGATGGCTCACAACCCTTGCAGCTGAGA20.5277044854881267No Hit
GTCACATGGATCCAAAAAGCACAGGATAGAACTCAATGGAGG20.5277044854881267No Hit
GGCGCTTGTAGAAGCGATGCCCACCCCAACTTCAGACCTACCGCAATCCAGTTTG20.5277044854881267No Hit
GTCACATGGATCCAAAAAGCACAGGATAGAACTCAATGGAGAG20.5277044854881267No Hit
TCCTGGCCTATCTGTTTCGTGTGGAC20.5277044854881267No Hit
TTCGGGACCACCCAACAGCGCCGCTAACTTCC20.5277044854881267No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCAGAAGG20.5277044854881267No Hit
AGGAACGAAACATTACAGATCATCAAAGAGAAGCTTGGGGAC20.5277044854881267No Hit
TGGATCCAAAAAGCACAGGTTAGAACTCAATGGAGAGGAAATG20.5277044854881267No Hit
AGCACAGGATAGAACTCAATGGAGAGG20.5277044854881267No Hit
CTTAGTTGGTGG20.5277044854881267No Hit
GTGACTCTGAGTTCTGTGGTTCCGGTTCAGACGATGAACAATTTCCTGGC20.5277044854881267No Hit
AAGCACAGGATAGAACTCAATGGAGAGGAAATGAAGAGGCCTTCGTCCAGGAA20.5277044854881267No Hit
AGAACTCAATGGAGAGGAAATGAAGAGGCCTTCGTCCAGCAGTGGACAG20.5277044854881267No Hit
CAAATACCGTATTCGGCTGCCGCCACGAAGT20.5277044854881267No Hit
AAGCACAGGATAGAACTCAATGGAGA20.5277044854881267No Hit
TCTATATGAGGC20.5277044854881267No Hit
TCCGGTTCAGACGATGAACAATTTCCTGGC20.5277044854881267No Hit
TTTTCAAGAAAG20.5277044854881267No Hit
CAGTTTGTTTCCTTTCACGGCGACCACCTCCACGTTGTCCACG20.5277044854881267No Hit
ACATTACAGATCATCAAAGAGAAGCTTGGGGAC20.5277044854881267No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCAGAAGGAACGAAACATTACAGATCATCAAAGAGAAGCT20.5277044854881267No Hit
ACTTCCAAGATTGCCTCAGCAAACAGTGACTCTGAGTTCTGTGGTTCCGGTTCAGACGATGAACAATT20.5277044854881267No Hit
TTGTTGGTGTCACATGGATCCAAAAAGCACAGGATAGAAC20.5277044854881267No Hit
CAAACAGTGACTCGGAGTTCTGTGGTTCC20.5277044854881267No Hit
TCCTGGCCTATCTGTTTCGTGTGGACGCCT20.5277044854881267No Hit
TTTCCTTTCACGGCGACCACCTCCACGTTGTCCACG20.5277044854881267No Hit
ACTTCCAAGATTGCCTCAGCAAACAGTGACTCTGAGTTCTGTGGTTCCGGTT20.5277044854881267No Hit
ACTTCCAAGATTGCCTCAGCAAACAGTGACTCTGAGTTCTGTGGTTCCGG20.5277044854881267No Hit
GCTGAACCGACGGCCTTAGAAACGTTGAAAACG20.5277044854881267No Hit
TCCTGGCCTATCTGTTTCGTGTGGACGCCTCGACAAACAGTGGAGCTTCT20.5277044854881267No Hit
AGATACGAAACTGCATCGTGGCACTT20.5277044854881267No Hit
CACATGGATCCAAAAAGCACAAGGATAGAACTCAATGGAGAG20.5277044854881267No Hit
AAGCACAGGATAGAACTCAATGGAGAGGAAATGAAGAGGCCTTCGTCCAG20.5277044854881267No Hit
GGAGAATGTAAACAAGATGTCGGAAGT10.2638522427440633No Hit
ATGAGAACGAC10.2638522427440633No Hit
CTATCTCCAAAAAGGTCGGCGACAGGCT10.2638522427440633No Hit
GCGCCGCTAACTTCCATTCTACAAACTCGTCATTGGTCCGAATCGAAAT10.2638522427440633No Hit
AAAGCACAGGATAGAACTCAATGGAGAGGAAATGAAGAGGCCTTCGTCCA10.2638522427440633No Hit
AAGAACAAGAAGGACTGGTGGCAAGAAGCCACTATGAGAACGACGGTGAC10.2638522427440633No Hit
CTGCTGCTGCTGGTGCTGTATTCTATAAAG10.2638522427440633No Hit
TTGCTCTATCTCCAAAAAGGTCGGCGACAGGCTGTGTACCTTCAAGTCGG10.2638522427440633No Hit
GCTGAACCGACGGCCTTAGAAACGTTGAAAACGT10.2638522427440633No Hit
TGCGAACGTCCAGCATGCTAACCAACTTGTTCAACAATTTTGCT10.2638522427440633No Hit
CGTGAGAAAGTTTGGTGGTGTCACATGGATCCAAAAAGCACAGGATAGAACTCA10.2638522427440633No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCAGAAGGAACGAAACATTACAGATCGTCAAAGAGT10.2638522427440633No Hit
GAGGATGAAATTATGTACTTTGGGGACAGA10.2638522427440633No Hit
ACAAGAAGGACTGGTGGCAAGAAGCCACTATGAGAACGACGGTGACTTTT10.2638522427440633No Hit
GATCCTTTTATGGCTTCGGGACCACCCAACAGCGCCGCTAACTTCCATTC10.2638522427440633No Hit
GGCGCTTGTAGAAGCGATGCCAACCCCAACTTCAGACCTACCGCAATTCA10.2638522427440633No Hit
GCACAGGATAGAACTCAATGGAGAGGAAATGAAGAGGCCTTCGTCC10.2638522427440633No Hit
AAGACACTCATTACCCGAACAGATCCTTTTAT10.2638522427440633No Hit
CCAAAAAGCACAGGATAGAACTCAAAAGAC10.2638522427440633No Hit
AGACCAAATACCGTATTCGGCTGCCGCCACGAAGAACAAGAAGGACT10.2638522427440633No Hit
TCGACAAACAGTGGAGCTTCTGCCAGCGGTATTTCTGCACGCCGCCAATCTAGCACGACGCAC10.2638522427440633No Hit
TGGGGACAGATACGAAACTGCATCGTGGCACTCCTTCAAGGAGTGCCTCCAAGGCC10.2638522427440633No Hit
ACCATGCTTAACGACTATTATGCGGACA10.2638522427440633No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCAGAAGGAACGAAACATTACAGA10.2638522427440633No Hit
CCGGTTCAGACGATGAACAATTTCCTGGCC10.2638522427440633No Hit
ACAGGATAGAACTCAATGGAAAGGAAATGAAGAGGCCTTCGTCCA10.2638522427440633No Hit
CACCCAACAGCGCCGCTAACTTCCATTCTACAAACTCGTCATTGGTC10.2638522427440633No Hit
TGGTGTCACATGGATCCAAAAAGCACAGGATAGAACTCAATGGAGAGGAAATGAAGAGGCCTTCGTCCAGCAG10.2638522427440633No Hit
AGACCAAATACCGTATTCGGCTGCCGCCACGAAGAACAAGAAGGACTG10.2638522427440633No Hit
GCGCCGCTAACTTCCATTCTACAAACTTGTCATTGGTCCGAATCGAAAT10.2638522427440633No Hit
TCGGCTGCCGCCACGAAGAACAAGAAGGACTGGTGGCAAGAAGCCACTAT10.2638522427440633No Hit
CGATGTGAGAAAGTTTGGTGGTGTCACATGGATCCAAAAAGC10.2638522427440633No Hit
CGATTGGGTAGTCAAATTAGACCAAATACCGTATTCGGCTGCCGCCACGAAGAACAAGAAGGACTGGTG10.2638522427440633No Hit
TGGATCCAAAAAGCACAGGATAGAACT10.2638522427440633No Hit
GGACGTGGAGTTGAAACGTCAGGTCACCAGAAGGAACGAAACATTACAGATCATCAAAGA10.2638522427440633No Hit
TATTTCTGCACGCCGCCAATCTAGCACGACGCACACAGGACGTGGAGTTG10.2638522427440633No Hit
ATACGAAACTGCATCGTGGCACTCCT10.2638522427440633No Hit
ATGATAAGTATTTTGAGCGACTGAATTGCCCGTGTTATTTTTGTAAACTTCCAAGATTGCCTCAGCAAACA10.2638522427440633No Hit
TATTGAGACACAGTTTCCCGCTACTGG10.2638522427440633No Hit
CATTCTACAAACTCGTCATTGGTCCGAA10.2638522427440633No Hit
GACGCCTCGACAAACAGTGGAGCTTCTGCCAGC10.2638522427440633No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCAGAAGGAACGAAACATTACAGATCATCAAAGA10.2638522427440633No Hit
AGGATAGAACTCAATGGAGAGGAAATGAAGAGGCCTTCGTCCAGCAGTGG10.2638522427440633No Hit
CAGGATAGAACTCAATGGAGAGGAAATGAAGAGGCCTTCGTCT10.2638522427440633No Hit
CACCTCCACGTCGTC10.2638522427440633No Hit
ATCTAGAACACCAGGTCACCCCGAG10.2638522427440633No Hit
CGCCTCGACAAACAGTGGAGCTTCTGCCC10.2638522427440633No Hit
AGAACAAGAAGGACTGGTGGCAAGAAGCCACTATGAGAACGACGGTGACTTTTGCAGCG10.2638522427440633No Hit
AGAACAAGAAGGACTGGTGGCAAGAAGCCACTATGAGAACGACGGTGACTTTTGCAGC10.2638522427440633No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCAGAAGGAACGAAACATTACAGATCATCA10.2638522427440633No Hit
AAACAGTGGAGCTTCTGCCAGCGGTATT10.2638522427440633No Hit
AAAAAGCACAGGATAGAACTCAATGGATGG10.2638522427440633No Hit
GACCACCCAACAGCGCCGCTAACTTCCATTCTACAAACTTGTCATTGGTCCGAATCGAAATTTGGCAC10.2638522427440633No Hit
ATCTAGAACACCAGATCACCCCGAGC10.2638522427440633No Hit
CTTTTTGTTGTTCAG10.2638522427440633No Hit
ACTTCCAAGATTGCCTCAGCAAACAGTGACTCGGAGTTCTGTGGTTCCG10.2638522427440633No Hit
AACCGTTGGTC10.2638522427440633No Hit
CCTCCACGTCGTCCACG10.2638522427440633No Hit
GTTACAACGATATGGAGATTGT10.2638522427440633No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCAGAAGGAACGAAACATTACAGATCATCAAAG10.2638522427440633No Hit
TCGGCTGCCGCCACGAAGAACAAGAAGGACT10.2638522427440633No Hit
GGCGTGACGATGTGAGAAAGTTTGGTGGTGTCACATGGATCCAAAAAGCACAGGATAGAACTCAATGGAGAG10.2638522427440633No Hit
AAACGTCAGGTCACCAGAAGGAACGAAACATTACAGATCGTCAAAGAGAAGCTTGGGGAC10.2638522427440633No Hit
TATTTCTGCACGCCGCCAATCTAGCACG10.2638522427440633No Hit
CATTGGGTCGTGCAACCGAAGTGTACAACAAAGCCCTGATGGACCACCTTCAGGCGGGGGAGGTGGGACCAC10.2638522427440633No Hit
ACATGGATCCAAAAAGCACAGGATAGAACTCAATGGAGAGAGAATGAAGAGGCCTTC10.2638522427440633No Hit
TAAACTTCCAAGATTGCCTCAGCAAACAGTGACTCTGAGTTCTGTGGTTC10.2638522427440633No Hit
GTGCTGTATTCT10.2638522427440633No Hit
ACAAAAAGCACAGGATAGAACTCAATGGAAAG10.2638522427440633No Hit
GTGGCAAGAAGCCACTATGAGAACGACGGTGACTTTTGCAGCGAATTCGGCATTGGGTCGTGCAACCGAAGT10.2638522427440633No Hit
ACGACGTATATTCCATACGCCATTCCGAAAGAAATTTGGACCTCCTTGATAGACAAATACG10.2638522427440633No Hit
AGTTTGTTTCCTTTCACGGCGACCACCTCCACGTTGTCCACG10.2638522427440633No Hit
TGATAAGTATTTTGAGCGACTGAATTGCCCGTGTTATTTTTGTAAACTTCCAAGATTGCCTCAGCAAACA10.2638522427440633No Hit
AAACTTCCAAGATTGCCTCAGCAAACAGTGACTCTGAGTTCTGTGGTTCC10.2638522427440633No Hit
TCACATGGATCCAAAAAGCACAGGATAGAACTCGA10.2638522427440633No Hit
ATTGCGCCTACACAAGCTCCACATGTCGTTGCGTCTTCATCAGGC10.2638522427440633No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCAGAAGGAACGAAACATTACAGATCGTCAAAGAGTAAATTTT10.2638522427440633No Hit
GATGTGAGAAAGTTTGGTGGTGTCACATGGATCCAAAAAGCACAGGATAGAACTCAATGGAG10.2638522427440633No Hit
GAACGAAACATTACAGATCATCAAAGAGAAGCTTGGGGAC10.2638522427440633No Hit
AGCTTGGGGACAGATACGAAACTGCAT10.2638522427440633No Hit
TAACGTTGGCAG10.2638522427440633No Hit
CCAAAAAGCACAGGATAAAACTCAATGGAGAGGAAATTAAGAGGCCTTCGTCCAGCAGTGGACAGG10.2638522427440633No Hit
TAGAACTCAATGGAGAGGAAATGAAGAGGCCTTGGTCCAGCGG10.2638522427440633No Hit
AGATACGAAACTGCATCGTGGCACT10.2638522427440633No Hit
TTTGAAAGAGTTACCTCTTCCGAAAGTCGTACCGAACT10.2638522427440633No Hit
AAATACCGTATTCGGCTGCCGCCACGAAGAACAAGAAGGACTGGTG10.2638522427440633No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCAGAAGGAACGAAACATTACAGATCGTCA10.2638522427440633No Hit
TCACATGGATCCAAAAAGCACAGGATAAAACTCAATGGAGAGGAAATTAAGAGGCCTTCGTC10.2638522427440633No Hit
CAGACGATGAACAATTTCCTGGCCTATC10.2638522427440633No Hit
CAGATACGAAACTGCATCGTGGCACTC10.2638522427440633No Hit
GTGTCACATGGATCCAAAAAGCACAGGATGGAACTCAATGGA10.2638522427440633No Hit
GTAAGATAGTGGCT10.2638522427440633No Hit
GGATCCAAAAAGCACAGGATAGAACTCAATGGA10.2638522427440633No Hit
CGCCGCCAATCTAGCACGACGCACACAGGACGTGGAGTTGAAACGTCAGGTCACCAGAAGGA10.2638522427440633No Hit
CCGCAATTCAGTTTGTTTCCTTTCACGGCGACCACC10.2638522427440633No Hit
ATGAGAACGACGGTGACTTTTGCGGCGAATTCGGCATTGGGTCGTGCAACCGAAGT10.2638522427440633No Hit
AGGAACGAAACATTACAGATCGTCAAAGAGAAGCTTGGGGAC10.2638522427440633No Hit
TTGGTGGGGAG10.2638522427440633No Hit
GCATACAAGACGCCCTCCGTACTCGACCCACCAAAAGGTGGGTTA10.2638522427440633No Hit
AGATACGAAACTGCATCGTGGCACTCCA10.2638522427440633No Hit
AGCGAATTCGGC10.2638522427440633No Hit
ATGAGAACGACGGTGACTTTTGCGGCGA10.2638522427440633No Hit
CAAACACAGAAGACGAGTGGGGAGCCTGG10.2638522427440633No Hit
GATTCGGAACCA10.2638522427440633No Hit
GTGGCAAGAAGCCACTATGAGAACGACGGTGACTTTTGCGGCGAATTCGGCATTGGGTC10.2638522427440633No Hit
TCCAAAAAGCACAGGATAGAACTCAAAG10.2638522427440633No Hit
GATGTGAGAAAGTTTGGTGGTGTCACATGGATCCAAAAAGCACAGG10.2638522427440633No Hit
GTATGAAATTATGTA10.2638522427440633No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCAGAAGGAACGAA10.2638522427440633No Hit
ACGCCTCGACAAACAGTGGAGCTTCTGCCAGCGGTATTTCTGC10.2638522427440633No Hit
TGTGGACGCCTCGACAAACAGTGGAGCTTCTGCCAGCGG10.2638522427440633No Hit
AGGTCGGCGACAGGCTGTGTACCTTCAAGTCGGAGGGCGTA10.2638522427440633No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCT10.2638522427440633No Hit
CCAATGTTACAA10.2638522427440633No Hit
TCGGGACCACCCAACAGCGCCGCTAACTTCC10.2638522427440633No Hit
AAACATTACAGATCATCAAAGAGAAGCTTGGGGAC10.2638522427440633No Hit
TACCTTCAAGTCGGAGGGCGTACATGGCGATTGGGTAGTCAAATTAGACC10.2638522427440633No Hit
TCGGCTGCCGCCACGAAGAACAAGAAGGACTG10.2638522427440633No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCAGAAGGAACGAAACATTACAGATCATCAAAGAGAAGCTTGGGGA10.2638522427440633No Hit
GAGAAGCTTGGGGACAGATACGAAACTGCATCGTGGCACTCCTTCAAGGAGTGCCTCCT10.2638522427440633No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCAGAAGGAACGAAACATTACAGATCATCAA10.2638522427440633No Hit
TCCTGGCCTATCTGTTTCGTGTGGACG10.2638522427440633No Hit
GCTGAACCGACGGCCTTCGAAACGT10.2638522427440633No Hit
TTCGACAATATCGATTCGGAACCAGTCGTGTCGTCAATACCTTGCTCTATCTCCAAAA10.2638522427440633No Hit
CCAAAAAGCACAGGATAGAACTCAATGGAGAGGAAATGAAGAGGCCTTTGTCCAGCAG10.2638522427440633No Hit
GAACGAAACAGTACAGATCATCAAAGAGAAGCTTGGGGAC10.2638522427440633No Hit
ATCCAGTTTGTTTCCTTTCACGGCGACCACCT10.2638522427440633No Hit
CAATTTCCTGGCCTATCTGTTTCGTGTGGACGCCTCGACAAACAGTGGAG10.2638522427440633No Hit
TTTATGGCTTCGGGACCACCCAACAGC10.2638522427440633No Hit
AGTTGAAACGTCAGGTCACCAGAAGGAACGAAACA10.2638522427440633No Hit
GAACAAGAAGGACTGGTGGCAAGAAGCCACTATGAGAACGACGGTGACTTTTGCGGCGAATTCGGCATTGGGTCG10.2638522427440633No Hit
CGGTTCAGACGATGAACAATTTCCTGGC10.2638522427440633No Hit
TTGGAATTGATGGACGTGTCAATGGTTGAATTATACAATAAGTATGTTTCCTTGG10.2638522427440633No Hit
ATAGAACTCAATGGAGAGGAAATGAAGAGGCCTTCGTCCAGCAGTGGACAGGTAACGGCTGACC10.2638522427440633No Hit
AACAGGTCGGA10.2638522427440633No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)