FastQCFastQC Report
Mon 16 Nov 2020
S34.raw.28821.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.raw.28821.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length11-54
%GC16

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAA1020.0No Hit
AAAAAAAAAAAA1020.0No Hit
AAAAAAAAAAAAAA510.0No Hit
AAAAAAAAAAAAA510.0No Hit
AAAAAAAAAAAAAAAAAAA36.0No Hit
ACTGGATTGAAAAAAAAAAAAAAAAA24.0No Hit
TAAAAAAAAAAAAAAAAAAAAA24.0No Hit
GTAAGTTCTGCCCCT24.0No Hit
AGACCTGAAGATCAAGGATGACCTCTTGCC24.0No Hit
AAAAAAAAAAAAAAAAAAAAA12.0No Hit
TGGACTTGTATGTACGGGGCTGAGT12.0No Hit
CTGTTATAAGTC12.0No Hit
CACGGTTCAGA12.0No Hit
GACATCTGGATACAAACTCTTAGTGAACGGAAGCATCATTGATGCAAGGCCTGG12.0No Hit
AGATGGGTGTTCACCCAGACATTGGGCC12.0No Hit
AAAAAAAAAAAAAAAAAAAAAA12.0No Hit
CCAGGCTTAACTTTGCATGTTGATGGCTCC12.0No Hit
TGAAGATCAAGGATGACCTCTTGCCAACC12.0No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)