##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	Panakeia.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	174482322
Sequences flagged as poor quality	0
Sequence length	20-151
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.0	37.0	37.0	37.0	37.0	37.0
2	36.14953631806895	37.0	37.0	37.0	37.0	37.0
3	36.3743751874187	37.0	37.0	37.0	37.0	37.0
4	36.403947323672135	37.0	37.0	37.0	37.0	37.0
5	36.456612756448756	37.0	37.0	37.0	37.0	37.0
6	36.473467810681704	37.0	37.0	37.0	37.0	37.0
7	36.48954845981474	37.0	37.0	37.0	37.0	37.0
8	36.48061142836006	37.0	37.0	37.0	37.0	37.0
9	36.5001685328328	37.0	37.0	37.0	37.0	37.0
10-14	36.498393456730824	37.0	37.0	37.0	37.0	37.0
15-19	36.49600985938277	37.0	37.0	37.0	37.0	37.0
20-24	36.47411584392962	37.0	37.0	37.0	37.0	37.0
25-29	36.435130434365	37.0	37.0	37.0	37.0	37.0
30-34	36.39544560564143	37.0	37.0	37.0	37.0	37.0
35-39	36.382434987298964	37.0	37.0	37.0	37.0	37.0
40-44	36.340655731063535	37.0	37.0	37.0	37.0	37.0
45-49	36.33128743881901	37.0	37.0	37.0	37.0	37.0
50-54	36.30859306910285	37.0	37.0	37.0	37.0	37.0
55-59	36.27489538798502	37.0	37.0	37.0	37.0	37.0
60-64	36.24900706589259	37.0	37.0	37.0	37.0	37.0
65-69	36.22662621599633	37.0	37.0	37.0	37.0	37.0
70-74	36.1982323211224	37.0	37.0	37.0	37.0	37.0
75-79	36.142739048338186	37.0	37.0	37.0	37.0	37.0
80-84	36.128996880316706	37.0	37.0	37.0	37.0	37.0
85-89	36.08019136116983	37.0	37.0	37.0	37.0	37.0
90-94	36.04094411829828	37.0	37.0	37.0	37.0	37.0
95-99	35.98100638159253	37.0	37.0	37.0	37.0	37.0
100-104	35.94347531942556	37.0	37.0	37.0	37.0	37.0
105-109	35.92326387766791	37.0	37.0	37.0	37.0	37.0
110-114	35.89762463306378	37.0	37.0	37.0	37.0	37.0
115-119	35.8417748090731	37.0	37.0	37.0	37.0	37.0
120-124	35.731359364913644	37.0	37.0	37.0	37.0	37.0
125-129	35.65292695308733	37.0	37.0	37.0	37.0	37.0
130-134	35.69605918295139	37.0	37.0	37.0	37.0	37.0
135-139	35.5407224766925	37.0	37.0	37.0	37.0	37.0
140-144	35.4949127753224	37.0	37.0	37.0	37.0	37.0
145-149	35.49663404724383	37.0	37.0	37.0	37.0	37.0
150-151	36.45119707994013	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	16.0
16	70.0
17	258.0
18	581.0
19	1186.0
20	2457.0
21	4960.0
22	9921.0
23	18277.0
24	29930.0
25	46644.0
26	73954.0
27	122618.0
28	214756.0
29	388986.0
30	711278.0
31	1330865.0
32	2404008.0
33	4531953.0
34	9212087.0
35	2.5738279E7
36	1.22490903E8
37	7148334.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	18.815880957842822	34.14490609541521	30.524273398883356	16.514939547858607
2	17.494875271936053	34.882881074829314	32.475657914541415	15.146585738693224
3	15.713127117810997	33.43184394534388	34.98267798874071	15.872350948104414
4	17.33392187843257	32.51513811672259	33.555560918724794	16.595379086120047
5	17.48731323558609	32.7137528422363	32.71358033105709	17.085353591120516
6	17.474466069013868	33.01972892473338	31.976597549546604	17.529207456706153
7	17.631728734828016	32.8350847562674	32.64999895290249	16.883187556002095
8	16.849677950178563	32.81739720104593	32.932266145933866	17.40065870284164
9	17.010026098976823	32.19599058630118	33.36454165307145	17.42944166165054
10-14	17.2240200550316	32.62131382519962	32.98168574097986	17.172980378788914
15-19	17.340470080275423	32.49775508838361	32.87942313245576	17.282351698885208
20-24	17.47139219264878	32.38493439766885	32.761422828910526	17.382250580771846
25-29	17.56111628306178	32.330966239177776	32.64299090743995	17.46492657032049
30-34	17.64072914770005	32.312498248269996	32.53205257832138	17.514720025708584
35-39	17.655738867243308	32.30489483297005	32.487733459288236	17.551632840498407
40-44	17.710993324320093	32.25960088405564	32.47013270531037	17.559273086313905
45-49	17.67864491714563	32.278357410710285	32.44403300297604	17.598964669168048
50-54	17.693178053974894	32.26647180761925	32.43463058607446	17.6057195523314
55-59	17.71476304382004	32.25506101200348	32.41607822948144	17.614097714695045
60-64	17.72195932400108	32.23441239043172	32.42204121525389	17.621587070313307
65-69	17.746995835033438	32.2095308253405	32.41427752665505	17.62919581297101
70-74	17.730978204870766	32.241842382941066	32.398561597039254	17.628617815148914
75-79	17.76152992829831	32.23127976321448	32.37605643302799	17.63113387545922
80-84	17.730205670518558	32.25673566321722	32.391681938141986	17.621376728122232
85-89	17.748137090796646	32.23256479456401	32.38916123751011	17.630136877129228
90-94	17.750487869685735	32.21649848676695	32.37847091082084	17.65454273272647
95-99	17.75073037173418	32.2257086424726	32.37283374762407	17.650727238169146
100-104	17.77744376162532	32.22953311836089	32.348190297284454	17.644832822729324
105-109	17.77706351400582	32.2132914497028	32.364353680014126	17.645291356277244
110-114	17.782900509399887	32.202336057480274	32.36519036553399	17.649573067585848
115-119	17.788484817417217	32.199235958817916	32.361086871222625	17.651192352542246
120-124	17.814423145494118	32.161675039510406	32.35377209445255	17.67012972054292
125-129	17.811640019082713	32.17274738547893	32.34708326327853	17.66852933215983
130-134	17.8310046623041	32.17619335700865	32.331578373273544	17.661223607413707
135-139	17.828712983154805	32.147505933065176	32.35411277571272	17.669668308067294
140-144	17.848752278970828	32.14681291768592	32.33527761968314	17.669157183660115
145-149	17.816993500704484	31.799143650555774	32.58483832433289	17.799024524406846
150-151	17.717219176626838	31.15372313546895	33.266239868132715	17.862817819771497
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10797.0
1	13310.0
2	20159.5
3	30943.0
4	48457.5
5	78123.0
6	129009.5
7	198077.5
8	277915.5
9	384043.5
10	534427.0
11	721583.5
12	940357.5
13	1188486.0
14	1441045.5
15	1653145.0
16	1807747.5
17	1936431.0
18	2053427.0
19	2161070.5
20	2276802.5
21	2427825.5
22	2623462.0
23	2862160.0
24	3153079.5
25	3474226.5
26	3803948.5
27	4141978.5
28	4487256.5
29	4894769.0
30	5384676.5
31	5833025.0
32	6129212.5
33	6281132.0
34	6349051.0
35	6377219.5
36	6340551.0
37	6287127.0
38	6281517.0
39	6246858.5
40	6113322.0
41	5884847.5
42	5660188.5
43	5344984.5
44	4849435.0
45	4339809.0
46	3911237.0
47	3553120.5
48	3202097.5
49	2861157.5
50	2566662.5
51	2309079.5
52	2070031.0
53	1849943.5
54	1652318.0
55	1448452.5
56	1235199.0
57	1035826.5
58	856714.5
59	693169.0
60	564476.5
61	468968.5
62	374684.5
63	290301.5
64	226768.5
65	175723.5
66	141286.5
67	120048.0
68	103824.5
69	84102.5
70	64280.0
71	49799.5
72	37516.0
73	27786.0
74	21212.0
75	18918.5
76	14949.5
77	11156.5
78	8079.0
79	4739.5
80	2920.0
81	2232.0
82	1705.5
83	1251.5
84	975.5
85	781.0
86	619.0
87	489.5
88	419.5
89	352.0
90	270.5
91	217.0
92	190.5
93	179.5
94	166.5
95	174.0
96	229.0
97	384.0
98	695.5
99	939.5
100	3534.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.08437416370467606
3	1.5703596608486215E-4
4	4.7569288996509345E-5
5	5.020565923005082E-4
6	4.6423041068882615E-5
7	2.2924958552534623E-6
8	1.1462479276267311E-6
9	1.7193718914400965E-6
10-14	1.3754975131520772E-5
15-19	9.995281928905096E-5
20-24	8.116115150180773E-5
25-29	0.003404781959789446
30-34	0.040447914861698686
35-39	0.0023469691437378085
40-44	0.03977031381967434
45-49	9.060437645871754E-5
50-54	2.987009832002502E-6
55-59	7.815889324846298E-6
60-64	1.3914682496861988E-5
65-69	8.238167341619372E-5
70-74	0.0028971775745801442
75-79	0.03756838657355458
80-84	0.0
85-89	0.0
90-94	3.462340074497533E-7
95-99	0.0
100-104	0.0
105-109	1.1567149515152807E-7
110-114	0.0
115-119	0.0
120-124	6.960054985826399E-7
125-129	4.761583849956277E-6
130-134	0.0
135-139	0.0
140-144	0.0
145-149	5.955592739735569E-7
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-24	41279.0
25-29	55281.0
30-34	59202.0
35-39	63998.0
40-44	69511.0
45-49	74623.0
50-54	79922.0
55-59	85576.0
60-64	90607.0
65-69	94977.0
70-74	100325.0
75-79	104440.0
80-84	108024.0
85-89	113898.0
90-94	120068.0
95-99	126688.0
100-104	133430.0
105-109	143920.0
110-114	156558.0
115-119	172421.0
120-124	191755.0
125-129	217588.0
130-134	284411.0
135-139	435366.0
140-144	979817.0
145-149	1.191942E7
150-152	1.58459217E8
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.2465996658213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.19235551878984	56.23758114788623
2	9.140297983166667	11.927467266679784
3	1.8854839260756318	3.6906424470299313
4	0.7138980572205949	1.8631768296671891
5	0.4119617602378818	1.3439552023934063
6	0.2545251881945058	0.9964141835396847
7	0.17817909609306076	0.8137906106121285
8	0.1423109648926063	0.7428245243523706
9	0.10867735544879821	0.638174511333711
>10	0.8538470323763913	10.911345652111322
>50	0.06854153997875906	3.064769457046018
>100	0.04728640752535879	5.707685334010333
>500	0.0016421473845101941	0.7310487158746123
>1k	9.73272242966377E-4	1.1951858868812908
>5k	7.652670809925095E-6	0.030636285960018734
>10k+	1.2097701630391281E-5	0.10530194462195679
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.731239638133656E-7	2.865619819066828E-6	5.731239638133656E-7	2.2924958552534623E-6	0.0
2	5.731239638133656E-7	4.584991710506925E-6	2.2924958552534623E-6	2.2924958552534623E-6	0.0
3	5.731239638133656E-7	6.877487565760387E-6	2.2924958552534623E-6	2.865619819066828E-6	0.0
4	2.2924958552534623E-6	7.450611529573752E-6	2.2924958552534623E-6	7.450611529573752E-6	5.731239638133656E-7
5	2.2924958552534623E-6	8.023735493387118E-6	2.865619819066828E-6	1.031623134864058E-5	5.731239638133656E-7
6	2.865619819066828E-6	9.16998342101385E-6	2.865619819066828E-6	1.2035603240080678E-5	2.2924958552534623E-6
7	4.584991710506925E-6	1.0889355312453945E-5	3.4387437828801934E-6	1.3181851167707408E-5	2.2924958552534623E-6
8	5.15811567432029E-6	1.1462479276267312E-5	4.011867746693559E-6	1.4901223059147504E-5	2.2924958552534623E-6
9	6.877487565760387E-6	1.3754975131520774E-5	4.011867746693559E-6	1.6047470986774237E-5	2.2924958552534623E-6
10-11	7.737173511480436E-6	1.4901223059147504E-5	5.15811567432029E-6	1.6907156932494285E-5	2.865619819066828E-6
12-13	8.310297475293801E-6	1.6620594950587602E-5	5.731239638133656E-6	1.862652882393438E-5	3.4387437828801934E-6
14-15	1.146247927626731E-5	1.8053404860121016E-5	5.731239638133656E-6	2.2351834588721256E-5	4.011867746693559E-6
16-17	1.3754975131520774E-5	2.0345900715374477E-5	5.731239638133656E-6	2.7223388281134865E-5	4.584991710506925E-6
18-19	1.5187785041054189E-5	2.3784644498254673E-5	6.3043636019470215E-6	2.9515884136388326E-5	4.584991710506925E-6
20-21	1.7766842878214333E-5	2.8083074226854913E-5	6.877487565760387E-6	3.3527751883081884E-5	6.017801620040339E-6
22-23	3.0375570082108378E-5	3.180837999164179E-5	7.450611529573752E-6	3.5820247738335345E-5	6.3043636019470215E-6
24-25	4.384398323172247E-5	3.49605617926153E-5	8.023735493387118E-6	3.725305764786876E-5	6.3043636019470215E-6
26-27	5.874520629086997E-5	3.639337170214872E-5	9.456545402920532E-6	4.040523944884227E-5	6.590925583853704E-6
28-29	7.565236322336425E-5	3.9545553503122224E-5	1.0029669366733897E-5	4.2984297286002416E-5	6.877487565760387E-6
30-31	9.055358628251177E-5	4.269773530409573E-5	1.1175917294360628E-5	4.7282727014602666E-5	6.877487565760387E-6
32-33	1.048816853778459E-4	4.5276793141255884E-5	1.1462479276267312E-5	5.15811567432029E-5	6.877487565760387E-6
34-35	1.3181851167707407E-4	4.7282727014602666E-5	1.1462479276267312E-5	5.530646250798978E-5	7.450611529573753E-6
36-37	1.5531659419342207E-4	4.986178485176281E-5	1.3468413149614091E-5	5.788552034514992E-5	8.023735493387118E-6
38-39	1.8397279238409036E-4	5.3873652598456364E-5	1.547434702296087E-5	6.017801620040338E-5	8.023735493387118E-6
40-41	2.0603806499090493E-4	5.645271043561651E-5	1.8053404860121016E-5	6.30436360194702E-5	8.310297475293801E-6
42-43	2.3068239543487962E-4	5.960489223659002E-5	1.8913090805841064E-5	6.47630079109103E-5	8.596859457200484E-6
44-45	2.6019827957126794E-4	6.161082610993681E-5	1.8913090805841064E-5	6.762862772997713E-5	9.16998342101385E-6
46-47	2.9114697361718974E-4	6.533613187472366E-5	2.0345900715374477E-5	7.078080953095065E-5	1.0889355312453945E-5
48-49	3.229553536088315E-4	6.705550376616377E-5	2.1205586661094525E-5	7.565236322336425E-5	1.0889355312453945E-5
50-51	3.625009071119537E-4	7.135393349476401E-5	2.177871062490789E-5	7.909110700624446E-5	1.146247927626731E-5
52-53	3.9660178295884897E-4	7.421955331383083E-5	2.2351834588721256E-5	8.367609871675138E-5	1.2895289185800726E-5
54-55	4.3012953484193085E-4	7.99507929519645E-5	2.2351834588721256E-5	8.912077637297834E-5	1.4041537113427456E-5
56-57	4.6738259248979965E-4	8.3102974752938E-5	2.3211520534441307E-5	9.284608213776523E-5	1.6047470986774237E-5
58-59	5.066415840110152E-4	8.596859457200484E-5	2.349808251634799E-5	9.685794988445878E-5	1.6620594950587602E-5
60-61	5.43894641658884E-4	8.768796646344493E-5	2.349808251634799E-5	9.972356970352561E-5	1.6907156932494285E-5
62-63	5.771358315600592E-4	8.854765240916499E-5	2.4071206480161355E-5	1.0201606555877908E-4	1.748028089630765E-5
64-65	6.138157652441146E-4	9.284608213776523E-5	2.464433044397472E-5	1.048816853778459E-4	1.8913090805841064E-5
66-67	6.610984922587172E-4	9.599826393873873E-5	2.5217454407788086E-5	1.0860699114263278E-4	2.0059338733467794E-5
68-69	7.009306077437462E-4	9.829075979399219E-5	2.579057837160145E-5	1.1347854483504638E-4	2.1205586661094525E-5
70-71	7.387567893554282E-4	1.0029669366733897E-4	2.66502643173215E-5	1.1691728861792659E-4	2.2065272606814573E-5
72-73	7.826007725871508E-4	1.014429415949657E-4	2.7509950263041548E-5	1.1978290843699341E-4	2.292495855253462E-5
74-75	8.187075823073927E-4	1.0258918952259243E-4	2.7509950263041548E-5	1.24654462129407E-4	2.4071206480161355E-5
76-77	8.485100284256877E-4	1.0688761925119268E-4	2.8656198190668278E-5	1.283797678941939E-4	2.464433044397472E-5
78-79	8.745871687791959E-4	1.0975323907025951E-4	2.9229322154481644E-5	1.3411100753232754E-4	2.5217454407788086E-5
80-81	8.992314992231705E-4	1.1290542087123302E-4	2.9515884136388326E-5	1.4012880915236788E-4	2.5217454407788086E-5
82-83	9.324726891243459E-4	1.166307266360199E-4	3.094869404592174E-5	1.4414067689906145E-4	2.5217454407788086E-5
84-85	9.72877928573188E-4	1.1892322249127336E-4	3.094869404592174E-5	1.464331727543149E-4	2.66502643173215E-5
86-87	0.0010184412836963506	1.2064259438271345E-4	3.152181800973511E-5	1.4987191653719509E-4	2.7509950263041548E-5
88-89	0.0010536884074708727	1.2408133816559362E-4	3.152181800973511E-5	1.5245097437435523E-4	2.7509950263041548E-5
90-91	0.0010834908535891676	1.2723351996656715E-4	3.2094941973548474E-5	1.5760909004867554E-4	2.8656198190668278E-5
92-93	0.001121030473218943	1.30099139785634E-4	3.2094941973548474E-5	1.6104783383155571E-4	2.980244611829501E-5
94-95	0.0011694594481611725	1.3067226374944736E-4	3.2954627919268525E-5	1.6534626356015598E-4	3.0375570082108374E-5
96-97	0.0012127303074290815	1.3267819762279414E-4	3.4387437828801935E-5	1.719371891440097E-4	3.066213206401506E-5
98-99	0.0012528489848960172	1.349706934780476E-4	3.524712377452199E-5	1.7766842878214333E-4	3.123525602782842E-5
100-101	0.0013029973317296867	1.3869599924283446E-4	3.5533685756428666E-5	1.8196685851074357E-4	3.152181800973511E-5
102-103	0.0013734915792787306	1.4098849509808795E-4	3.725305764786876E-5	1.851190403117171E-4	3.152181800973511E-5
104-105	0.0014325233475515073	1.4385411491715474E-4	3.782618161168213E-5	1.914234039136641E-4	3.152181800973511E-5
106-107	0.0014786598266384831	1.4987191653719509E-4	3.897242953930886E-5	1.9543527166035766E-4	3.152181800973511E-5
108-109	0.0015586106195904477	1.54456908247702E-4	3.897242953930886E-5	1.9887401544323787E-4	3.1808379991641795E-5
110-111	0.0016391345365062255	1.5588971815723543E-4	3.9545553503122224E-5	2.0259932120802473E-4	3.266806593736184E-5
112-113	0.001729114998824924	1.5789565203058221E-4	3.9545553503122224E-5	2.0517837904518487E-4	3.3241189901175205E-5
114-115	0.0018036211141206615	1.59901585903929E-4	3.9545553503122224E-5	2.0947680877378514E-4	3.381431386498857E-5
116-117	0.0018798466013078391	1.6219408175918246E-4	3.983211548502891E-5	2.1406180048429206E-4	3.381431386498857E-5
118-119	0.0019681076917350973	1.6420001563252924E-4	4.040523944884227E-5	2.1664085832145217E-4	3.4100875846895256E-5
120-121	0.0020517837904518487	1.6763875941540942E-4	4.0691801430748954E-5	2.2122585003195912E-4	3.4673999810708615E-5
122-123	0.0021380389470057604	1.7079094121638295E-4	4.097836341265564E-5	2.2724365165199947E-4	3.524712377452199E-5
124-125	0.0022480787480579265	1.7480280896307648E-4	4.1838049358375685E-5	2.315420813805997E-4	3.610680972024203E-5
126-127	0.0023366264004670914	1.796743626554901E-4	4.1838049358375685E-5	2.3526738714538655E-4	3.66799336840554E-5
128-129	0.002433770912333457	1.8339966842027697E-4	4.1838049358375685E-5	2.398523788558935E-4	3.725305764786876E-5
130-131	0.0024827730112394995	1.8540560229362375E-4	4.32708592679091E-5	2.4386424660258703E-4	3.868586755740218E-5
132-133	0.002516014201140675	1.8769809814887724E-4	4.441710719553583E-5	2.4816267633118725E-4	3.9545553503122224E-5
134-135	0.0025472494571685033	1.9085027994985074E-4	4.527679314125588E-5	2.5418047795122765E-4	4.011867746693559E-5
136-137	0.002569314729775318	1.945755857146376E-4	4.584991710506924E-5	2.6191765146270807E-4	4.0691801430748954E-5
138-139	0.0025698878537391313	1.9744120553370445E-4	4.6709603050789294E-5	2.66502643173215E-4	4.0691801430748954E-5
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
