##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	Panakeia.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	174482322
Sequences flagged as poor quality	0
Sequence length	20-151
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.0	37.0	37.0	37.0	37.0	37.0
2	35.58454089692823	37.0	37.0	37.0	37.0	37.0
3	35.74265877204454	37.0	37.0	37.0	37.0	37.0
4	35.72123433799786	37.0	37.0	37.0	37.0	37.0
5	35.76146860310582	37.0	37.0	37.0	37.0	37.0
6	35.733997361635296	37.0	37.0	37.0	37.0	37.0
7	35.73259235970048	37.0	37.0	37.0	37.0	37.0
8	35.7110308974453	37.0	37.0	37.0	37.0	37.0
9	35.699524751854234	37.0	37.0	37.0	37.0	37.0
10-14	35.69564901365767	37.0	37.0	37.0	37.0	37.0
15-19	35.65565181898485	37.0	37.0	37.0	37.0	37.0
20-24	35.640020012595706	37.0	37.0	37.0	37.0	37.0
25-29	35.57505996352131	37.0	37.0	37.0	37.0	37.0
30-34	35.54069418433518	37.0	37.0	37.0	37.0	37.0
35-39	35.49883158429138	37.0	37.0	37.0	37.0	37.0
40-44	35.44986983156772	37.0	37.0	37.0	37.0	37.0
45-49	35.37914125235643	37.0	37.0	37.0	37.0	37.0
50-54	35.34083227418796	37.0	37.0	37.0	37.0	37.0
55-59	35.3012524514302	37.0	37.0	37.0	32.2	37.0
60-64	35.24057503065679	37.0	37.0	37.0	25.0	37.0
65-69	35.20066309298212	37.0	37.0	37.0	25.0	37.0
70-74	35.124576670063284	37.0	37.0	37.0	25.0	37.0
75-79	35.07790190955509	37.0	37.0	37.0	25.0	37.0
80-84	34.99619229198622	37.0	37.0	37.0	25.0	37.0
85-89	34.950368873946886	37.0	37.0	37.0	25.0	37.0
90-94	34.90334950797328	37.0	37.0	37.0	25.0	37.0
95-99	34.82391607729471	37.0	37.0	37.0	25.0	37.0
100-104	34.741333445731115	37.0	37.0	37.0	25.0	37.0
105-109	34.72935921120653	37.0	37.0	37.0	25.0	37.0
110-114	34.706456388947814	37.0	37.0	37.0	25.0	37.0
115-119	34.65184757687161	37.0	37.0	37.0	25.0	37.0
120-124	34.58331010385661	37.0	37.0	37.0	25.0	37.0
125-129	34.385280041160314	37.0	37.0	37.0	25.0	37.0
130-134	34.32982450592372	37.0	37.0	37.0	25.0	37.0
135-139	34.29730119404189	37.0	37.0	37.0	25.0	37.0
140-144	34.22174665672679	37.0	37.0	37.0	25.0	37.0
145-149	34.248586725562184	37.0	37.0	37.0	25.0	37.0
150-151	35.943295453109016	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	465.0
13	3194.0
14	6256.0
15	7740.0
16	8629.0
17	10178.0
18	13242.0
19	20199.0
20	32578.0
21	57025.0
22	95801.0
23	157236.0
24	234067.0
25	343505.0
26	503019.0
27	771129.0
28	1195168.0
29	1917661.0
30	3275999.0
31	5548279.0
32	8442114.0
33	1.2977157E7
34	2.1252556E7
35	4.3287846E7
36	7.2671768E7
37	1649510.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	18.333442971947612	35.242640798877034	29.88894141378976	16.534974815385596
2	17.739424580112377	35.78374670047329	31.51089474038733	14.96593397902701
3	15.9743949073213	34.225508153344656	34.068703155318524	15.731393784015516
4	17.489047400458137	32.8939180440297	32.940154246686376	16.676880308825783
5	17.423595497542728	33.02802962468599	32.358067197202935	17.190307680568353
6	17.4490632609224	33.236975906188135	31.587476328259566	17.7264845046299
7	17.59827372737944	32.98016434791175	32.32341631921498	17.09814560549383
8	16.82136772572295	33.0048742703	32.65103383940523	17.522724164571812
9	17.007478941089968	32.43706163216387	33.03742388892225	17.518035537823913
10-14	17.20009213818075	32.950623566353	32.657736635190815	17.19154766027544
15-19	17.29775532311626	32.74387978462143	32.59410666899025	17.364258223272063
20-24	17.425154213587295	32.69871721765868	32.46176509077884	17.414363477975183
25-29	17.506408810688733	32.61076067571516	32.353098877635205	17.5297316359609
30-34	17.57047630145564	32.63157682235403	32.23499736723212	17.562949508958212
35-39	17.598605835578155	32.60484746619425	32.18070980735009	17.615836890877496
40-44	17.627040576114055	32.58079074428275	32.17475259187324	17.61741608772995
45-49	17.62007625840877	32.58610590925714	32.13194269638128	17.661875135952812
50-54	17.634553413898814	32.573637642122556	32.12763579309791	17.664173150880718
55-59	17.651996478914374	32.564575038019605	32.11381813298957	17.669610350076457
60-64	17.658224290370242	32.53800185701598	32.12472408392319	17.67904976869059
65-69	17.6819313424429	32.51562270157701	32.11584129356198	17.686604662418112
70-74	17.667009532595866	32.54711942179113	32.103298154698514	17.68257289091449
75-79	17.68586956060808	32.53630964432287	32.090537091590214	17.687283703478833
80-84	17.674109322549004	32.55232308038653	32.09605464522804	17.67751295183643
85-89	17.696367974447575	32.525938206782214	32.09765293439338	17.680040884376833
90-94	17.703883791731418	32.50532930636133	32.08301372820229	17.707773173704965
95-99	17.7055226390348	32.50601117123949	32.08230182359823	17.706164366127474
100-104	17.73831199854819	32.50785372458036	32.055906672042475	17.697927604828976
105-109	17.73811228408953	32.48655820580119	32.07719628123208	17.698133228877204
110-114	17.750384733340315	32.48063918678779	32.06561647790537	17.70335960196652
115-119	17.758693653086144	32.47281257678799	32.067536134204246	17.700957635921622
120-124	17.78971076822452	32.44109394350217	32.05628403396041	17.71291125431291
125-129	17.814326839182172	32.428320421701	32.047944944044865	17.70940779507196
130-134	17.835448014877166	32.42446770404275	32.03398896786484	17.706095313215243
135-139	17.828366787193687	32.4132118024125	32.05598705283181	17.702434357562012
140-144	17.84885851474682	32.41261848969388	32.042398109268845	17.696124886290463
145-149	17.790222768039044	32.086302383108965	32.320426686008524	17.803048162843467
150-151	17.430383732100708	31.775797502399538	33.22620791414768	17.56761085135208
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41082.0
1	38237.0
2	39980.0
3	51502.5
4	70914.5
5	103076.5
6	156158.5
7	228006.5
8	312592.5
9	422797.5
10	574288.5
11	762532.5
12	981196.0
13	1228469.5
14	1480282.5
15	1693126.0
16	1853641.5
17	1987168.5
18	2109162.5
19	2219753.0
20	2332650.0
21	2483525.5
22	2678149.0
23	2914363.5
24	3205578.5
25	3526286.5
26	3855627.5
27	4194892.5
28	4547348.0
29	4958995.0
30	5435347.0
31	5865722.0
32	6149058.0
33	6301303.5
34	6374047.0
35	6403649.5
36	6371138.5
37	6317523.0
38	6301434.0
39	6250198.0
40	6111283.5
41	5889588.5
42	5667684.0
43	5350218.0
44	4859752.5
45	4357770.5
46	3933297.0
47	3572156.0
48	3219929.0
49	2881175.0
50	2583937.5
51	2322722.0
52	2086193.0
53	1867105.5
54	1667879.5
55	1469084.0
56	1260618.0
57	1064659.5
58	884914.5
59	723594.5
60	595770.5
61	496155.5
62	401215.0
63	315651.0
64	249139.5
65	193844.0
66	155886.5
67	133358.0
68	114802.0
69	93572.0
70	74743.0
71	60064.5
72	45684.0
73	34045.5
74	25933.0
75	22255.0
76	18085.0
77	14057.0
78	10533.0
79	7020.5
80	4951.0
81	3958.0
82	3367.0
83	2831.5
84	2386.0
85	2055.5
86	1859.5
87	1757.0
88	1643.5
89	1581.5
90	1555.5
91	1561.0
92	1616.0
93	1698.5
94	1926.0
95	2327.5
96	2864.5
97	3761.5
98	21808.0
99	65848.5
100	129087.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	5.788552034514993E-5
3	5.731239638133656E-7
4	0.0
5	0.0
6	5.731239638133656E-6
7	1.83399668420277E-5
8	0.0
9	5.731239638133656E-7
10-14	2.3154208138059968E-5
15-19	3.782618161168213E-6
20-24	2.75241678335356E-6
25-29	3.444805382987009E-7
30-34	0.0
35-39	1.3926826895370239E-5
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	2.3464709732401107E-7
110-114	2.3503633753442454E-6
115-119	2.1191417119109115E-6
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	1.0782981664298083E-6
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-24	222518.0
25-29	210840.0
30-34	202586.0
35-39	202711.0
40-44	209130.0
45-49	210180.0
50-54	213977.0
55-59	217880.0
60-64	224513.0
65-69	230244.0
70-74	237134.0
75-79	239655.0
80-84	243436.0
85-89	248724.0
90-94	256305.0
95-99	263984.0
100-104	269173.0
105-109	279256.0
110-114	290697.0
115-119	331328.0
120-124	366878.0
125-129	441668.0
130-134	536892.0
135-139	831830.0
140-144	1759220.0
145-149	1.5037158E7
150-152	1.50704405E8
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.14311119663775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.34115815506644	58.643570940487486
2	8.342005666232504	11.202164281016623
3	1.6910298126097056	3.406230082346488
4	0.6832661070339155	1.8350644880588791
5	0.3892531246030608	1.3067832914431
6	0.23268073161399402	0.9373744941644055
7	0.16793485694330748	0.7892968141475163
8	0.13206806538103016	0.7093968639522665
9	0.10989585055616975	0.664087438254779
>10	0.8035152121778074	10.381030848021572
>50	0.06169841765372192	2.86303462683991
>100	0.043053214450133485	5.225652711551669
>500	0.0015288133265475843	0.7059677263357154
>1k	8.938851790356699E-4	1.1455319985447756
>5k	6.397403395626614E-6	0.03166100211408808
>10k+	1.1689769217449491E-5	0.1531523927211563
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.731239638133656E-7	1.1462479276267311E-6	0.0	0.0	0.0
2	1.7193718914400967E-6	2.2924958552534623E-6	5.731239638133656E-7	1.1462479276267311E-6	0.0
3	2.2924958552534623E-6	3.4387437828801934E-6	5.731239638133656E-7	2.2924958552534623E-6	0.0
4	4.011867746693559E-6	5.731239638133656E-6	1.1462479276267311E-6	3.4387437828801934E-6	5.731239638133656E-7
5	4.584991710506925E-6	6.877487565760387E-6	1.1462479276267311E-6	4.584991710506925E-6	5.731239638133656E-7
6	5.15811567432029E-6	8.023735493387118E-6	1.1462479276267311E-6	5.731239638133656E-6	5.731239638133656E-7
7	5.731239638133656E-6	8.596859457200484E-6	1.1462479276267311E-6	6.877487565760387E-6	5.731239638133656E-7
8	5.731239638133656E-6	1.1462479276267312E-5	1.1462479276267311E-6	8.023735493387118E-6	5.731239638133656E-7
9	7.450611529573752E-6	1.6047470986774237E-5	1.1462479276267311E-6	9.743107384827215E-6	5.731239638133656E-7
10-11	8.596859457200484E-6	1.7193718914400968E-5	2.2924958552534623E-6	1.432809909533414E-5	2.2924958552534623E-6
12-13	8.883421439107166E-6	2.0059338733467794E-5	4.298429728600242E-6	1.748028089630765E-5	2.579057837160145E-6
14-15	1.0889355312453945E-5	2.3211520534441307E-5	4.584991710506925E-6	2.4930892425881403E-5	2.865619819066828E-6
16-17	1.232216522198736E-5	2.8656198190668278E-5	6.590925583853704E-6	2.8083074226854913E-5	4.298429728600242E-6
18-19	1.4041537113427456E-5	3.180837999164179E-5	7.1640495476670696E-6	2.980244611829501E-5	4.871553692413607E-6
20-21	2.0345900715374477E-5	3.6966495665962076E-5	8.023735493387118E-6	3.266806593736184E-5	5.15811567432029E-6
22-23	3.4387437828801935E-5	4.040523944884227E-5	1.0889355312453945E-5	3.725305764786876E-5	6.590925583853704E-6
24-25	5.673927241752319E-5	4.1838049358375685E-5	1.0889355312453945E-5	4.1551487376469E-5	6.877487565760387E-6
26-27	8.396266069865806E-5	4.49902311593492E-5	1.1749041258173995E-5	4.556335512316256E-5	6.877487565760387E-6
28-29	1.2092915636462014E-4	4.699616503269598E-5	1.2035603240080678E-5	4.8715536924136076E-5	8.310297475293801E-6
30-31	1.6477313959634263E-4	5.2727404670829634E-5	1.2035603240080678E-5	5.559302448989646E-5	1.0029669366733897E-5
32-33	2.097633707556918E-4	5.7312396381336557E-5	1.3468413149614091E-5	5.788552034514992E-5	1.0602793330547263E-5
34-35	2.6019827957126794E-4	5.845864430896329E-5	1.4328099095334139E-5	6.046457818231006E-5	1.146247927626731E-5
36-37	3.112063123506575E-4	5.960489223659002E-5	1.6047470986774237E-5	6.218395007375017E-5	1.2608727203894043E-5
38-39	3.6393371702148715E-4	6.103770214612343E-5	1.6047470986774237E-5	6.504956989281699E-5	1.4041537113427456E-5
40-41	4.2038642745710365E-4	6.33301980013769E-5	1.6047470986774237E-5	6.791518971188382E-5	1.547434702296087E-5
42-43	4.825703775308538E-4	6.504956989281699E-5	1.6047470986774237E-5	7.164049547667069E-5	1.547434702296087E-5
44-45	5.539243110256179E-4	6.791518971188382E-5	1.7193718914400968E-5	7.479267727764422E-5	1.547434702296087E-5
46-47	6.321557320861423E-4	7.278674340429743E-5	1.862652882393438E-5	8.023735493387118E-5	1.547434702296087E-5
48-49	7.092409052190399E-4	7.622548718717761E-5	1.8913090805841064E-5	8.310297475293801E-5	1.6047470986774237E-5
50-51	7.883320122252844E-4	7.966423097005782E-5	1.948621476965443E-5	8.568203259009815E-5	1.748028089630765E-5
52-53	8.737274828334758E-4	8.109704087959123E-5	1.948621476965443E-5	8.82610904272583E-5	1.7766842878214333E-5
54-55	9.634213831702675E-4	8.568203259009815E-5	2.0059338733467794E-5	9.227295817395186E-5	1.948621476965443E-5
56-57	0.001055121217380406	8.883421439107166E-5	2.177871062490789E-5	9.628482592064541E-5	2.177871062490789E-5
58-59	0.001154558225102025	9.255952015585855E-5	2.2351834588721256E-5	1.0258918952259243E-4	2.2351834588721256E-5
60-61	0.0012476908692216969	9.513857799301869E-5	2.2351834588721256E-5	1.048816853778459E-4	2.3211520534441307E-5
62-63	0.0013471278769433158	9.68579498844588E-5	2.2351834588721256E-5	1.0774730519691272E-4	2.4071206480161355E-5
64-65	0.0014425530169182412	9.886388375780556E-5	2.3211520534441307E-5	1.1290542087123302E-4	2.4071206480161355E-5
66-67	0.0015391244048207932	1.0287575150449911E-4	2.349808251634799E-5	1.1720385059983327E-4	2.464433044397472E-5
68-69	0.001625952685338518	1.06601057269286E-4	2.3784644498254673E-5	1.2178884231034018E-4	2.464433044397472E-5
70-71	0.001722810635222977	1.097532390702595E-4	2.4930892425881403E-5	1.2666039600275377E-4	2.4930892425881403E-5
72-73	0.0018036211141206615	1.1175917294360628E-4	2.6077140353508134E-5	1.315319496951674E-4	2.5217454407788086E-5
74-75	0.0018543425849181444	1.1405166879885974E-4	2.6936826299228182E-5	1.3382444555042086E-4	2.5217454407788086E-5
76-77	0.0018904493946383864	1.1777697456364663E-4	2.6936826299228182E-5	1.3783631329711442E-4	2.5217454407788086E-5
78-79	0.0019331471299424822	1.2035603240080677E-4	2.6936826299228182E-5	1.4385411491715474E-4	2.5217454407788086E-5
80-81	0.0019787104850656446	1.229350902379669E-4	2.779651224494823E-5	1.4987191653719509E-4	2.5217454407788086E-5
82-83	0.0020300050798269407	1.2580071005703375E-4	2.8083074226854913E-5	1.5101816446482183E-4	2.550401638969477E-5
84-85	0.002077001244859637	1.283797678941939E-4	2.9229322154481644E-5	1.5417034626579536E-4	2.6936826299228182E-5
86-87	0.002121131790073266	1.3210507365898075E-4	2.9515884136388326E-5	1.6104783383155571E-4	2.7223388281134865E-5
88-89	0.0021775845005088824	1.3411100753232754E-4	2.980244611829501E-5	1.6477313959634263E-4	2.8083074226854913E-5
90-91	0.0022300253431978053	1.3640350338758102E-4	3.123525602782842E-5	1.719371891440097E-4	2.8369636208761596E-5
92-93	0.0022907764833620223	1.3926912320664782E-4	3.152181800973511E-5	1.7738186680023662E-4	2.980244611829501E-5
94-95	0.0023532469954176786	1.4385411491715474E-4	3.1808379991641795E-5	1.799609246373968E-4	2.980244611829501E-5
96-97	0.0024352037222429906	1.447138008628748E-4	3.266806593736184E-5	1.8368623040218368E-4	2.980244611829501E-5
98-99	0.0025363601018560493	1.4586004879050152E-4	3.49605617926153E-5	1.8999059400413068E-4	3.094869404592174E-5
100-101	0.0026077140353508135	1.4757942068194165E-4	3.524712377452199E-5	1.945755857146376E-4	3.1808379991641795E-5
102-103	0.0026624473738949897	1.5101816446482183E-4	3.5820247738335345E-5	1.9715464355179774E-4	3.2094941973548474E-5
104-105	0.0027312222495525936	1.533106603200753E-4	3.725305764786876E-5	2.0030682535277127E-4	3.2094941973548474E-5
106-107	0.002839829240695226	1.5760909004867554E-4	3.782618161168213E-5	2.034590071537448E-4	3.4100875846895256E-5
108-109	0.0029404124963444724	1.6076127184964906E-4	3.839930557549549E-5	2.0718431291853165E-4	3.4673999810708615E-5
110-111	0.0030312526446088905	1.6563282554206264E-4	3.9545553503122224E-5	2.0947680877378514E-4	3.524712377452199E-5
112-113	0.0031091975036875085	1.6763875941540942E-4	4.011867746693559E-5	2.1348867652047868E-4	3.5820247738335345E-5
114-115	0.0032100673213186605	1.7021781725256956E-4	4.097836341265564E-5	2.1549461039382546E-4	3.66799336840554E-5
116-117	0.0033364411553395076	1.7336999905354309E-4	4.1838049358375685E-5	2.19506478140519E-4	3.725305764786876E-5
118-119	0.0034588031216136613	1.7480280896307648E-4	4.212461134028237E-5	2.229452219233992E-4	3.725305764786876E-5
120-121	0.00355652075744384	1.7680874283642326E-4	4.269773530409573E-5	2.2609740372437273E-4	3.725305764786876E-5
122-123	0.003675157417953207	1.813937345469302E-4	4.384398323172246E-5	2.2924958552534625E-4	3.782618161168213E-5
124-125	0.0038253158964723084	1.8311310643837032E-4	4.441710719553583E-5	2.3268832930822643E-4	3.868586755740218E-5
126-127	0.0039837846724667045	1.859787262574371E-4	4.7569288996509345E-5	2.3670019705491997E-4	3.897242953930886E-5
128-129	0.004076917316586376	1.8855778409459725E-4	5.043490881557617E-5	2.4042550281970688E-4	3.9545553503122224E-5
130-131	0.0041465518781897	1.9199652787747746E-4	5.186771872510959E-5	2.458701804759338E-4	4.126492539456232E-5
132-133	0.004219338621593997	1.931427758051042E-4	5.416021458036305E-5	2.4758955236737394E-4	4.212461134028237E-5
134-135	0.004325079992917563	1.9428902373273092E-4	5.645271043561651E-5	2.501686102045341E-4	4.269773530409573E-5
136-137	0.004360613678673992	1.9658151958798438E-4	5.702583439942987E-5	2.5504016389694766E-4	4.32708592679091E-5
138-139	0.0043631927365111525	2.0030682535277127E-4	5.81720823270566E-5	2.61057965516988E-4	4.355742124981578E-5
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
