FastQCFastQC Report
Wed 8 Jan 2025
SRR2125761.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125761.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34272875
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length20-75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGGCGCTGGAACCCGGGGTGTTGTACTCTC1829550.5338186539646879No Hit
GGTAAGACGGAGGTCGGGGGTTCGATCCCCCCAGTGGCCTCCA1397860.407861902452012No Hit
AACGTGTCGCGATGGATGACTTGGCTTCCTATTTCGTTGAAGAACGCAGC1281130.3738028980644314No Hit
ATGGCGCTGGAACCCGGGGTGTTGTACTCTC1064900.310712188574784No Hit
CTAAGCCAGAAACCAGTCCCAAGACCGGGTGCCCGT904060.26378294788517154No Hit
GGCGCTGGAACCCGGGGTGTTGTACTCTC854530.24933128603888643No Hit
ATACATTAATACAAAATCAGACTATGTGAGTTAGATGACGTGAATTATTT845250.24662360540223136No Hit
ATACAATCATACACAGTAGTACATATGTAGTGATAGACGTGATTAATTTT732530.21373462249665368No Hit
AAGACGCTAAGGGTTGGATTGTTCACCCACT567020.16544278821079353No Hit
AAGTTCTACAGTCCGACGATC544320.15881947458449283Illumina DpnII expression Sequencing Primer (95% over 21bp)
ATATATACAATAACAGTGGTAAGGTAA533210.15557784399470426No Hit
TTGGGCGTTTGAGGGTTGGTGACCCGTGGGCGCCCAGCCGTGCGGAACT527210.15382718841066004No Hit
ATATATAACAACATAAAATACTATGTGATGTAGGATCTGTGAATTAATTT514730.15018582479584805No Hit
GGGTCGGTGTGTTGTGTGTGGGTT501530.1463343825109507No Hit
ATATATAAAACTACGAGTAGATTCTATGATTGATGAACGTGTAAATTTTT500720.14609804400710474No Hit
CCCCGGTGGAGGCCTGTAGCGATGCTGACGT489330.14277471615672743No Hit
GGCAAGTGCTCCGACGCTATCGCACGGTGGTTCTCGGCTGAACG485620.14169222745392676No Hit
GAAGACGCTAAGGGTTGGATTGTTCACCCACT485330.14160761243403128No Hit
TGGGCGTTTGAGGGTTGGTGACCCGTGGGCGCCCAGCCGTGCGGAACT481080.14036756472866663No Hit
ATATATAACGACACAATAGAGAAAGATGCTCTGAGAGATGTAATATTTTT452030.13189147394258577No Hit
ATATATTAAAACATATATTAGTATTTCGGTACATAGAGCTCACGTTTTTT425390.1241185631494294No Hit
ATACATAAATAGATAAGATAGTATATGATGAGACTGTGATGATTGTAATT402040.11730559516819059No Hit
CCTCTAAGCCAGAAACCAGTCCCAAGACCGGGTGCCCGT402020.1172997596495771No Hit
GGTCGGTGTGTTGTGTGTGGGTT395310.11534194315475431No Hit
GTCGGTGTGTTGTGTGTGGGTT392280.11445786208481197No Hit
ATGACCTCGAACTGTGGCACCATTTTTGTGGCCGTTCTTATAGGGCTGGT365300.1065857474752264No Hit
CGGTGTGTTGTGTGTGGGTT360830.10528150906511345No Hit
ATATATATAACTAACGAATTACTATTTTGATATCTTGGACGTCGAATT348380.10164889872822165No Hit
GCTCCCATAGTGTAGCGGTTATCACCTCGGACT345720.10087277475262872No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)