FastQCFastQC Report
Wed 8 Jan 2025
SRR2125763.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125763.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23485983
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC5592342.3811394226079448RNA PCR Primer, Index 12 (100% over 50bp)
TCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCCTTGTCGG2050000.8728610592965175No Hit
ATCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCCTTGTCG1862780.7931454263592033No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT1847260.7865372294615048RNA PCR Primer, Index 12 (100% over 36bp)
ACGGAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGC1212050.5160737789855336No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT1153900.4913143299132934RNA PCR Primer, Index 12 (100% over 35bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG1046300.4454997689472908RNA PCR Primer, Index 12 (100% over 37bp)
GACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAA993830.4231587836881258No Hit
TCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAG915510.389811233364173No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC824150.35091143513132916RNA PCR Primer, Index 2 (100% over 34bp)
CGGAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCG820170.3492168073186462No Hit
CACGGAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCG810290.3450100427987196No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC770550.32808931182484463RNA PCR Primer, Index 1 (100% over 33bp)
AACCACGGAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGG640040.27251999628885026No Hit
ACAATGTCGCTCCCGCTTGATCAAAAGAGCGGGGAAACCACGGAATCACG637040.27124263864109927No Hit
CCACGGAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTC632920.26948840080485453No Hit
ACCACGGAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGT610600.2599848599055871No Hit
AGTGGACTGCCATGGCGTTGACGGGAGCGGGGGATTAGGGTTCGATTCCG538260.22918350915948463No Hit
CCCGCTTGATCAAAAGAGCGGGGAAACCACGGAATCACGTAGACCCACTT523570.22292871454433053No Hit
TGAAAAGGGGCAACAGAGAACCTGGGATGATATTCCAAAAAGAAATTGCA510670.21743607665900125No Hit
ATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCAAC502490.21395314813946686No Hit
AATGTCGCTCCCGCTTGATCAAAAGAGCGGGGAAACCACGGAATCACGTA486270.20704690112395974No Hit
AGACGTGTTGACTCAATTCATTCCGTGCGAAAGCCGAGCCTTGTTGCTCG468040.19928482448445953No Hit
CGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGG445800.18981534645579876Illumina Small RNA Adapter 2 (100% over 21bp)
ACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCAACGAG431390.18367977188776813No Hit
AATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCAA411290.17512147564783642No Hit
CACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCAACGA399980.17030583731581514No Hit
TCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCAACG396610.16887093889150817No Hit
TTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACT376460.1602913533574473No Hit
AGTGCATGGTTGTTTTCAATGGGGGATACTCAAACCCATCCAATATCGAG363490.1547689104603371No Hit
CACAATGTCGCTCCCGCTTGATCAAAAGAGCGGGGAAACCACGGAATCAC342610.14587850123199017No Hit
TGCTGGCGACAATAGCAAGAGTGAACCACCTGATCCCTTCCCGAACTCAG333560.14202513899460797No Hit
AAAAGAGCGGGGAAACCACGGAATCACGTAGACCCACTTGGGACCGAGTA331910.14132259228834493No Hit
ACGCACTTGTCGTGGCGATGGGACGTCCAGCGAATGAATGAAATTAGAAC330000.1405093412526101No Hit
CCGCTTGATCAAAAGAGCGGGGAAACCACGGAATCACGTAGACCCACTTG329640.14035605833488No Hit
ATGTCGCTCCCGCTTGATCAAAAGAGCGGGGAAACCACGGAATCACGTAG326840.13916385786364574No Hit
CAATGTCGCTCCCGCTTGATCAAAAGAGCGGGGAAACCACGGAATCACGT326160.13887432346348882No Hit
GTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAA309190.13164873703604402No Hit
AAACCACGGAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTG306230.13038841082359637No Hit
CTCCCGCTTGATCAAAAGAGCGGGGAAACCACGGAATCACGTAGACCCAC297440.12664575291568592No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATG294870.1255514831974459RNA PCR Primer, Index 12 (100% over 49bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA290470.12367802531407777RNA PCR Primer, Index 12 (100% over 39bp)
GTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCAACGAGGA284920.12131491366573842No Hit
GTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGG268730.11442144022670885No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG266800.11359967347332237RNA PCR Primer, Index 12 (100% over 45bp)
ACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAG266680.11354857916741233No Hit
CTTGATCAAAAGAGCGGGGAAACCACGGAATCACGTAGACCCACTTGGGA265620.11309724613187364No Hit
GAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCA252390.10746409890529172No Hit
CGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCAACGAGG251850.10723417452869655No Hit
TAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTG247900.10555232029249106No Hit
AGGGGCAACAGAGAACCTGGGATGATATTCCAAAAAGAAATTGCATGTGG244020.10390027106806643No Hit
ACGGAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTTGGAATTCT237190.10099215349001998No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)