FastQCFastQC Report
Wed 8 Jan 2025
SRR2125764.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125764.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16908019
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC7546674.463367352497061RNA PCR Primer, Index 3 (100% over 50bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA2691021.5915643340594778RNA PCR Primer, Index 3 (100% over 36bp)
GTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGG2283791.3507141197321815No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG1679880.9935404023380858RNA PCR Primer, Index 3 (100% over 37bp)
TCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAG1473340.8713853467990542No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT1437590.8502415333221474RNA PCR Primer, Index 3 (100% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1430400.8459891132130853RNA PCR Primer, Index 3 (100% over 34bp)
TTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACT1413370.8359169693386315No Hit
AATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAA1094570.6473673823054019RNA PCR Primer, Index 1 (100% over 23bp)
GACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAA957110.5660686801925169No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC721680.42682705762277656RNA PCR Primer, Index 1 (100% over 33bp)
ACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAG689780.40796027021261333No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC651350.38523141001911576RNA PCR Primer, Index 3 (100% over 39bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT631370.3734145318857283RNA PCR Primer, Index 3 (100% over 43bp)
TGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCA623970.369037910354844No Hit
TGCTGGCGACAATAGCAAGAGTGAACCACCTGATCCCTTCCCGAACTCAG547160.32360976173494954No Hit
TAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTG527490.3119762285575856No Hit
AGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGG511530.3025369204990839No Hit
ATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAAC465380.2752421794652585RNA PCR Primer, Index 1 (100% over 24bp)
TTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATTGCTC464980.2750056053284539No Hit
GCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGC454740.26894930742625733No Hit
CTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCT435510.2575760057993784No Hit
CGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGG421880.24951474208776322Illumina Small RNA Adapter 2 (100% over 21bp)
TGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTG414230.24499026172137608No Hit
GCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC374640.2215753365311454No Hit
TGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAAT362620.21446628372016852No Hit
GAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCT340070.2011294167578118No Hit
GATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAA339570.20083369908680612No Hit
TTGTAATCAGTAGGTCCACAGTTCGAATCCGTGTGCCGGCACCATGGAAT328910.1945289983409647No Hit
GTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAA326890.1933342989501017No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATG317010.18749091777102925RNA PCR Primer, Index 3 (100% over 49bp)
ACGTTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAAT294460.1741540508086725No Hit
GTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGGA289680.17132698987385808No Hit
ATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAAG266720.15774763442127668No Hit
ATCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCCTTGTCG260520.1540807353008061No Hit
CCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTAT256050.1514370193220152No Hit
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGT254690.1506326672568797RNA PCR Primer, Index 3 (100% over 46bp)
TAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATT253000.14963314152888046No Hit
TTCCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTT251220.1485803866201002No Hit
TGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTA245310.14508500374881292No Hit
ACGACGTTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACC240390.1421751418661169No Hit
ATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAAT239710.14177296583354915No Hit
AGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTG239210.14147724816254348No Hit
CCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTATC230250.13617798749812143No Hit
ATAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTT230080.13607744348997952No Hit
AACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTC225490.13336275527014727No Hit
AACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTATCCAT224830.13297240794441975No Hit
AAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGT220670.1305120369216524No Hit
AGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGC219420.12977274274413816No Hit
AGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATTG217990.12892699020506188No Hit
CTTCCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGT215720.12758443197869604No Hit
GGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCC215260.12731237172137078No Hit
CGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTC210640.12457994044127818No Hit
CAGTAATGCATTAAGCTAACCAGTACTAATTGCCCGTGCGGCTTGACCTG207860.12293575019048655No Hit
CAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGA203060.1200968605488319RNA PCR Primer, Index 1 (100% over 22bp)
CTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAA197750.11695633888275142No Hit
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC196970.11649501931598255RNA PCR Primer, Index 3 (98% over 50bp)
CGCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCT195220.11546000746746263No Hit
TATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAA194260.1148922295391317No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG190920.11291683549681368RNA PCR Primer, Index 3 (100% over 38bp)
TTAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTT190200.11249100205056548No Hit
CCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGG189690.11218937002613967Illumina Small RNA Adapter 2 (100% over 21bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGTGGAATTCTCGGGTG180860.10696699595617915No Hit
AGACGTGTTGACTCAATTCATTCCGTGCGAAAGCCGAGCCTTGTTGCTCG178450.1055416367819317No Hit
CAGTAATGCATTAAGCTAACCAGTACTAATTGCCCGTGCGGCTTGACTGG174400.1031463236467856No Hit
TAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTA170440.10080423969242051No Hit
TCCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTA170180.10065046650349756No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)