FastQCFastQC Report
Wed 8 Jan 2025
SRR2125765.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125765.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8770644
Total Bases885.8 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC89019510.14971078520574RNA PCR Primer, Index 10 (100% over 50bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG3228133.680607718201765RNA PCR Primer, Index 10 (100% over 36bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC3029923.454615191313203RNA PCR Primer, Index 10 (100% over 37bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA1717411.9581344311774596RNA PCR Primer, Index 10 (100% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1641761.871880787773395RNA PCR Primer, Index 3 (100% over 34bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1012631.1545674411137883RNA PCR Primer, Index 1 (100% over 33bp)
TCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAG930521.0609483180482528No Hit
GACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAA909671.0371758333823606No Hit
AATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAA592500.6755490246782334RNA PCR Primer, Index 1 (100% over 23bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATG471850.5379878604125308RNA PCR Primer, Index 10 (100% over 49bp)
GTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGG470820.5368134882683643No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT468110.5337236353453635RNA PCR Primer, Index 10 (100% over 39bp)
ATCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCCTTGTCG433460.4942168442819022No Hit
TTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACT412560.470387351259497No Hit
CGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGG406180.463113084968447Illumina Small RNA Adapter 2 (100% over 21bp)
TCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCCTTGTCGG388460.4429093234202643No Hit
ACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAG311780.3554813078720331No Hit
TGCTGGCGACAATAGCAAGAGTGAACCACCTGATCCCTTCCCGAACTCAG275520.3141388477288555No Hit
GTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAA270880.3088484722444555No Hit
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGT270370.3082669870080236RNA PCR Primer, Index 10 (100% over 46bp)
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT249100.2840156321474227RNA PCR Primer, Index 10 (100% over 41bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA230930.26329879539062356RNA PCR Primer, Index 1 (100% over 32bp)
TAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTG224430.2558877090439425No Hit
CTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCT209200.23852296365010367No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT208870.23814670849711833RNA PCR Primer, Index 10 (100% over 38bp)
TGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCA207070.23609440766265283No Hit
AGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGG202450.230826835520858No Hit
ATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAAC201310.22952704499236315RNA PCR Primer, Index 1 (100% over 24bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACTAGCTTATCTCGTATGCC179900.20511606673352606RNA PCR Primer, Index 10 (97% over 35bp)
GAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCT178540.20356543943637434No Hit
TTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATTGCTC175490.20008792968908554No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG168090.19165069292517173RNA PCR Primer, Index 10 (100% over 45bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT145350.16572329238309066RNA PCR Primer, Index 10 (100% over 43bp)
TGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAAT142380.1623369960062226No Hit
TGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTG139000.15848323110594845No Hit
TTGTAATCAGTAGGTCCACAGTTCGAATCCGTGTGCCGGCACCATGGAAT133820.15257716537120877No Hit
CGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC133770.15252015701469585RNA PCR Primer, Index 10 (100% over 42bp)
GCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGC124720.14220164448585532No Hit
GATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAA124700.14217884114325013No Hit
ACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACC118400.13499578822262082No Hit
AACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTC118020.13456252471312255No Hit
AGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTG116230.13252162554995964No Hit
TTCCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTT113090.12894150076094754No Hit
CCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTAT113030.12887309073313202No Hit
CACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCAC111190.12677518321345616No Hit
GCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC110130.12556660605538203No Hit
TAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAG109810.12520175257369925No Hit
GTCCCTATCGTCTAGAGGCCTAGGACATCGCCCTTTCACGGCGGTAACCG107630.12271618822973546No Hit
CGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTC107070.12207769463679063No Hit
CGCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCT104530.11918167012593374No Hit
AAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGT103000.11743721441663806No Hit
GAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCG102370.11671890912457511No Hit
ATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAAG101180.11536211023956736No Hit
TTGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC100850.11498585508658202RNA PCR Primer, Index 10 (98% over 50bp)
TATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAA97200.11082424506113804No Hit
ACGTTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAAT94760.108042237263307No Hit
TGGAATTCACGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC91340.10414286567782252RNA PCR Primer, Index 10 (98% over 50bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT91040.10380081553874494RNA PCR Primer, Index 10 (100% over 41bp)
CAGTAATGCATTAAGCTAACCAGTACTAATTGCCCGTGCGGCTTGACTGG90700.103413158714457No Hit
GATAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTG90290.10294569019105096No Hit
TGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTA90170.10280887013541992No Hit
AGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGC89620.10218177821377768No Hit
ATAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGA87840.10015228072191734No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)